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Conserved GC-boxes, E-box and GATA motif are essential for GATA-4 gene expression in P19CL6 cells.

Abstract：

:The promoter of the GATA-4 gene was analyzed in P19CL6 cells. A 124bp segment containing conserved two GC-boxes and E-box was essential for the basal promoter activity, as determined with a transient luciferase reporter gene assay. However, an extended 1312 bp reporter construct but not the 124 bp segment, when ligated to the GFP gene and stably inserted into the chromosome, showed regulated promoter activity since GFP was expressed upon DMSO addition. Mutations of the two GC-boxes and/or E-box significantly impaired the GFP expression. Furthermore, mutation of the distal conserved GATA motif in the 1312 bp sequence decreased the expression of GFP. Chromatin immuno-precipitation assay showed that GATA-6 binds to this conserved GATA motif. These results suggest that the distal GATA motif recognized by GATA-6 together with the GC- and E-boxes may be important for transcriptional activation of the GATA-4 gene in the chromosome.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ishibashi T,Yokura Y,Ohashi K,Yamamoto H,Maeda M

doi

10.1016/j.bbrc.2011.08.036

subject

Has Abstract

pub_date

2011-09-23 00:00:00

pages

171-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)01421-5

journal_volume

413

pub_type

杂志文章

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