Differential expression of two forms of annexin 3 in human neutrophils and monocytes and along their differentiation.

abstract：:Fractionation of a petroleum ether extract of Helianthus annuus L. led to the isolation of three diterpene acids: grandiflorolic, kaurenoic and trachylobanoic acids. These compounds were studied for potential anti-inflammatory activity on the generation of inflammatory mediators in lipopolysaccharide (LPS)-activated R...

journal_title：Biochemical and biophysical research communications

authors： Díaz-Viciedo R,Hortelano S,Girón N,Massó JM,Rodriguez B,Villar A,de Las Heras B

更新日期：2008-05-02 00:00:00

abstract：:We have compared the coupling of human alpha(1A)-, alpha(1B)-, and alpha(1D)-adrenoceptors (expressed at approximately 2000 fmol/mg protein in Chinese hamster ovary cells) to cellular growth promotion (as assessed by [(3)H]thymidine incorporation) and related signaling mechanisms. Maximum elevation of intracellular Ca...

journal_title：Biochemical and biophysical research communications

authors： Keffel S,Alexandrov A,Goepel M,Michel MC

更新日期：2000-06-16 00:00:00

abstract：:Jmjd2c is a candidate oncogene that encodes histone lysine demethylase. In this study, we discovered that over-expression of Jmjd2c increased the expression of Mdm2 oncogene dependent on its demethylase activity, which led to the reduction of p53 tumor suppressor gene product in the cells. A chromatin immunoprecipitat...

journal_title：Biochemical and biophysical research communications

authors： Ishimura A,Terashima M,Kimura H,Akagi K,Suzuki Y,Sugano S,Suzuki T

更新日期：2009-11-13 00:00:00

abstract：:Small GTP-binding proteins of the Ras and Rho family participate in various important signalling pathways. Large clostridial cytotoxins inactivate GTPases by UDP-glucosylation. Using Clostridium difficile toxin B-10463 (TcdB) for inactivation of Rho proteins (RhoA/Rac/Cdc42) and Clostridium sordellii lethal toxin-1522...

journal_title：Biochemical and biophysical research communications

authors： Hippenstiel S,Kratz T,Krüll M,Seybold J,von Eichel-Streiber C,Suttorp N

更新日期：1998-04-28 00:00:00

abstract：:Murine interleukin 6 (mIL-6) has been synthesized as a fusion protein using a lac operon inducible plasmid in Escherichia coli. The first 8 amino acids are from the N-terminus of bacterial beta-galactosidase and the last 175 amino acids are from residue number 12 to the end of native mIL-6. This fusion protein is equi...

journal_title：Biochemical and biophysical research communications

authors： Simpson RJ,Moritz RL,Van Roost,Van Snick J

更新日期：1988-11-30 00:00:00

abstract：:ASC2 structure has been well defined by 1141 NOE experimental restraints. The model consists of five alpha helices. alpha-Helices are connected by short random structure loops. The sole exception is the loop connecting helices 2 and 3, which has a 20-residue length. Folding generally agrees with the folding of recentl...

journal_title：Biochemical and biophysical research communications

authors： Espejo F,Patarroyo ME

更新日期：2006-02-17 00:00:00

abstract：:Having ascertained using JC-1 as a probe that, in distinction with the controls, during capacitation boar sperm maintains high mitochondrial membrane potential (ΔΨ), to gain some insight into the role of mitochondria in capacitation, we monitored ΔΨ generation due to externally added metabolites either in hypotonicall...

journal_title：Biochemical and biophysical research communications

authors： Paventi G,Lessard C,Bailey JL,Passarella S

更新日期：2015-07-03 00:00:00

abstract：:The transcription of a mouse genomic segment containing four tRNA genes, coding for a tRNA(Ala), a tRNA(Ile), a tRNA(Pro) and a tRNA(Lys), has been studied in a HeLa cell extract, demonstrating that differences among their transcriptional efficiencies are evident using as templates either the natural cluster or an equ...

journal_title：Biochemical and biophysical research communications

authors： Russo T,Oliva A,Duilio A,Ammendola R,Costanzo F,Zannini M,Cimino F

更新日期：1987-12-31 00:00:00

abstract：:Using an anti-serum against synthetic alpha-inhibin-31, we have studied its cellular locations in rodent testis and brain. Western blot analysis detected several molecular forms of the protein(s). A protein of 17 KDa in size was detected in testis of one month old mice. Two major bands of molecular weights 39 kDA and ...

journal_title：Biochemical and biophysical research communications

authors： Lau YF,Li CH

更新日期：1987-05-29 00:00:00

abstract：:In this study, we examined the endothelin (ET) receptor subtype involved in mitogenic signaling in human primary and metastatic melanoma cell lines. In a reverse transcriptase-polymerase chain reaction (RT-PCR) study, ET(B) mRNA expression in metastatic melanoma cells was decreased from that of primary melanoma. Only ...

journal_title：Biochemical and biophysical research communications

authors： Kikuchi K,Nakagawa H,Kadono T,Etoh T,Byers HR,Mihm MC,Tamaki K

更新日期：1996-02-27 00:00:00

abstract：:The yeast Saccharomyces cerevisiae mRNA capping enzyme is composed of two subunits of alpha (52 kDa, mRNA guanylyltransferase) and beta (80 kDa, RNA 5'-triphosphatase). We have isolated the alpha subunit gene (CEG1) by immunological screening. In this report, with the aid of partial amino acid sequences of purified ye...

journal_title：Biochemical and biophysical research communications

authors： Tsukamoto T,Shibagaki Y,Imajoh-Ohmi S,Murakoshi T,Suzuki M,Nakamura A,Gotoh H,Mizumoto K

更新日期：1997-10-09 00:00:00

abstract：:Secretion of growth hormone (GH) exhibits marked sexual dimorphism in the rat. To examine the underlying mechanism that involves hypothalamic GH-releasing factor (GRF), we determined pituitary GRF receptor mRNA levels in male and female rats and compared their in vitro abilities to release GRF, an endogenous ligand fo...

journal_title：Biochemical and biophysical research communications

authors： Ono M,Miki N,Murata Y,Osaki E,Tamitsu K,Ri T,Yamada M,Demura H

更新日期：1995-11-22 00:00:00

abstract：:To investigate the role of the carboxyl group in deltorphin molecules, we have synthesized three new analogues in which the acidic amino acid residues in position 4 of the deltorphins were replaced by non-acidic but hydrophilic amino acids residues. The three analogues, [Ser4]-, [Gln4]-, and [Cys4]-deltorphin, all are...

journal_title：Biochemical and biophysical research communications

authors： Misicka A,Lipkowski AW,Fang L,Knapp RJ,Davis P,Kramer T,Burks TF,Yamamura HI,Carr DB,Hruby VJ

更新日期：1991-11-14 00:00:00

abstract：:Total kininogen in plasma of Freund's adjuvant treated rats increased 20-fold 7 days following the injection. Analysis of the kininogens demonstrated that increases in T-kininogen was the major reason for the rise in kininogen. High molecular weight and low molecular weight kininogens showed little or no change. The i...

journal_title：Biochemical and biophysical research communications

authors： Barlas A,Okamoto H,Greenbaum LM

更新日期：1985-05-31 00:00:00

abstract：:The newly identified drug transporter MRP is functionally linked to a multiple drug resistance independent from P-glycoprotein. Resistance modifiers for this type of MDR are rare at present. We analyzed the modulating effect of the highly selective bisindolylmaleimide PKC inhibitor GF 109203X on the MRP overexpressing...

journal_title：Biochemical and biophysical research communications

authors： Gekeler V,Boer R,Ise W,Sanders KH,Schächtele C,Beck J

更新日期：1995-01-05 00:00:00

abstract：:The mechanism of tumour necrosis factor-mediated cytotoxicity was investigated by using various inhibitors of arachidonic acid metabolism. Phospholipase A2 inhibitors with different modes of action interfered with the cytotoxic action of TNF, whereas phospholipase C inhibitors did not. Neither cyclooxygenase nor lipox...

journal_title：Biochemical and biophysical research communications

authors： Suffys P,Beyaert R,Van Roy F,Fiers W

更新日期：1987-12-16 00:00:00

abstract：:The expression of sucrase-isomaltase mRNA was investigated along the crypt-villus axis of rat small intestine using differentially isolated cells and in situ hybridization. A partial rat sucrase-isomaltase cDNA was cloned which coded for a protein that was predicted to be 88% homologous to those encoded by the rabbit ...

journal_title：Biochemical and biophysical research communications

authors： Traber PG

更新日期：1990-12-31 00:00:00

abstract：:Very-long-chain acyl-CoA synthetases (VLCS) activate very-long-chain fatty acids (VLCFA) containing 22 or more carbons to their CoA derivatives. We cloned the human ortholog (hVLCS) of the gene encoding the rat liver enzyme (rVLCS). Both hVLCS and rVLCS contain 620 amino acids, are expressed primarily in liver and kid...

journal_title：Biochemical and biophysical research communications

authors： Steinberg SJ,Wang SJ,Kim DG,Mihalik SJ,Watkins PA

更新日期：1999-04-13 00:00:00

abstract：:Papiliocin is a 37-residue peptide isolated from the swallowtail butterfly Papilio xuthus. In this study, we found that Papiliocin induced the accumulation of reactive oxygen species (ROS) and hydroxyl radicals known to be important regulators of apoptosis in Candida albicans. To examine the relationship between the a...

journal_title：Biochemical and biophysical research communications

authors： Hwang B,Hwang JS,Lee J,Kim JK,Kim SR,Kim Y,Lee DG

更新日期：2011-04-29 00:00:00

abstract：:The development of central nervous system (CNS) neuronal networks involves processes including neuroblast migration, axonal pathfinding, and synaptogenesis. To evaluate the role of the axonal guidance molecule DSCAM in CNS connectivity, we generated a lacZ reporter construct, Pr1.8-betagal, containing a 1.8kb fragment...

journal_title：Biochemical and biophysical research communications

authors： Barlow GM,Lyons GE,Richardson JA,Sarnat HB,Korenberg JR

更新日期：2002-11-22 00:00:00

abstract：:An exposure of a human myeloma cell line to 2-difluoromethylornithine the mechanism-based inhibitor of ornithine decarboxylase (EC 4.1.1.17), resulted in a selection of tumor cells readily growing in the presence of 4 mM difluoromethylornithine, a concentration that swiftly halted the growth of the parental cells. Det...

journal_title：Biochemical and biophysical research communications

authors： Alhonen-Hongisto L,Leinonen P,Laine R,Jänne J

更新日期：1987-04-14 00:00:00

abstract：:Transformation of fluorene by washed cells of fluorene-grown Pseudomonas sp. F274 yielded 1,la-dihydroxy-1-hydrofluoren-9-one (up to 100 mg/l) as the stable product of angular dioxygenation of 9-fluorenone. Structural identity of the angular keto-diol was established by 13C- and 1H-NMR, gas chromatography- and direct ...

journal_title：Biochemical and biophysical research communications

authors： Selifonov SA,Grifoll M,Gurst JE,Chapman PJ

更新日期：1993-05-28 00:00:00

abstract：:Prostate cancer (CaP) bone metastasis is an early event that remains inactive until later-stage progression. Reduced levels of circulating androgens, due to andropause or androgen deprivation therapies, alter androgen receptor (AR) coactivator expression. Coactivators shift the balance towards enhanced AR-mediated gen...

journal_title：Biochemical and biophysical research communications

authors： Villagran MA,Gutierrez-Castro FA,Pantoja DF,Alarcon JC,Fariña MA,Amigo RF,Muñoz-Godoy NA,Pinilla MG,Peña EA,Gonzalez-Chavarria I,Toledo JR,Rivas CI,Vera JC,McNerney EM,Onate SA

更新日期：2015-11-27 00:00:00

abstract：:Sphingolipids are essential components of eukaryotic cell membranes. We recently showed that the function of the serotonin(1A) receptor is impaired upon metabolic depletion of sphingolipids using fumonisin B(1) (FB(1)), a specific inhibitor of ceramide synthase. Serotonin(1A) receptors belong to the family of G-protei...

journal_title：Biochemical and biophysical research communications

authors： Ganguly S,Paila YD,Chattopadhyay A

更新日期：2011-07-22 00:00:00

abstract：:Parkinson's disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Prevailing evidence suggests that abnormal autophagy and mitochondrial dysfunction participate in the process of PD. However, many damages of neuronal functions are regulated b...

journal_title：Biochemical and biophysical research communications

authors： Zhao M,Chen J,Mao K,She H,Ren Y,Gui C,Wu X,Zou F,Li W

更新日期：2019-02-05 00:00:00

abstract：:Inducible nitric oxide (NO) synthase (iNOS), heme oxygenase (HO)-1, and indoleamine 2,3-dioxygenase (IDO) are simultaneously expressed in murine macrophages stimulated with interferon (IFN)-gamma and lipopolysaccharide (LPS). NO produced by iNOS suppresses IDO expression and also induces HO-1 expression. The antioxida...

journal_title：Biochemical and biophysical research communications

authors： Oh GS,Pae HO,Choi BM,Chae SC,Lee HS,Ryu DG,Chung HT

更新日期：2004-08-06 00:00:00

abstract：:The importance of Trp 800 in the calmodulin-binding site of myosin light chain kinase was investigated. Truncation mutants from Leu 447 to the C-terminus were expressed in E. coli and these were modified by point mutations of Trp 800 to Gly, Cys, Leu and Tyr. Trp at this position was more effective than any of the oth...

journal_title：Biochemical and biophysical research communications

authors： Matsushima S,Huang YP,Dudas CV,Guerriero V Jr,Hartshorne DJ

更新日期：1994-08-15 00:00:00

abstract：:Previous research showed that protein kinase C alpha (PKC alpha) translocated to the perinuclear region and activated phospholipase D1, but the mechanism involved was not clear. Here, we provide evidence that Phe 663 (the 10th amino acid from C-terminus) of PKC alpha is essential for its translocation. A point mutatio...

journal_title：Biochemical and biophysical research communications

authors： Hu T,Exton JH

更新日期：2005-08-05 00:00:00

abstract：:Using isolated rat hepatocytes we have shown that glutathione (GSH) depletion by glutathione-S-transferase (GST)-catalyzed conjugation with 1-bromoheptane or phorone was accompanied by a significant elevation in ascorbate synthesis. Glycogenolysis was also stimulated without a significant rise in glucose synthesis. Fu...

journal_title：Biochemical and biophysical research communications

authors： Chan TS,Wilson JX,O'Brien PJ

更新日期：2004-04-23 00:00:00

abstract：:The self-assembly of amyloid-beta (Aβ) proteins in aqueous extracellular environments is implicated in Alzheimer's disease. Among several alloforms of Aβ proteins differing in sequence length, the 42- and 40-residue forms (Aβ42 and Aβ40) are the most abundant ones in the human body. Although the only difference is the...

journal_title：Biochemical and biophysical research communications

authors： Lin Y,Im H,Diem LT,Ham S

更新日期：2019-03-12 00:00:00