Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress.

abstract：:Most formal methods for analyzing the divergent evolution of protein sequences assume a Markov model where position i in a polypeptide chain undergoes amino acid substitution independently from position i + 1. The large number of aligned homologous sequence pairs available from the exhaustive matching of the protein s...

journal_title：Biochemical and biophysical research communications

authors： Gonnet GH,Cohen MA,Benner SA

更新日期：1994-03-15 00:00:00

abstract：:Clostridium difficile toxin A (TcdA) is one of the main pathogenic factors released by C. difficile. Due to its potent cytotoxic and proinflammatory activities, we investigated the anti-tumor activity of TcdA. CT26 colorectal cancer cells were challenged with recombinant TcdA, and it was found that TcdA could induce a...

journal_title：Biochemical and biophysical research communications

authors： Tian Y,Huang T,Li G,Liu J,Wang X,Feng H,Wang J

更新日期：2012-05-25 00:00:00

abstract：:Phosphofructokinase (PFK) from human polymorphonuclear leukocytes (PMN) was characterized by immunological titration with subunit specific antibodies and column chromatography on QAE-Sephadex in three different groups: control, type II diabetic, and obese individuals. It was found that PMN phosphofructokinase in the t...

journal_title：Biochemical and biophysical research communications

authors： Durante P,Raleigh X,Gómez ME,Campos G,Ryder E

更新日期：1996-08-23 00:00:00

abstract：:The characterization of a 20 kilodalton (20 kD) variant of rat growth hormone is reported. The 20 kD variant from rat pituitary gland extracts was identified on Western immunoblots of polyacrylamide gels. It was also shown that pituitary tissue maintained in culture secretes the 20 kD form. A rat growth hormone cDNA f...

journal_title：Biochemical and biophysical research communications

authors： Howland DS,Farrington MA,Taylor WD,Hymer WC

更新日期：1987-09-15 00:00:00

abstract：:Saposins (SAPs) are small glycoproteins required for activation of sphingolipid hydrolysis by lysosomal enzymes. Four SAPs, SAP-A, -B, -C, and -D, are proteolytically cleaved from a single gene product termed prosaposin. The mature coding sequence of human SAP-A tagged with 6-histidine was expressed in Pichia pastoris...

journal_title：Biochemical and biophysical research communications

authors： Yamada M,Inui K,Hamada D,Nakahira K,Yanagihara K,Sakai N,Nishigaki T,Ozono K,Yanagihara I

更新日期：2004-05-28 00:00:00

abstract：:Glutaredoxin 3 (GLRX3) is a member of monothiol glutaredoxins with a CGFS active site that has been demonstrated to function in cellular iron sensing and trafficking via its bound iron-sulfur cluster. Human GLRX3 has been shown to form a dimer that binds two bridging [2Fe-2S] clusters with glutathione (GSH) as a ligan...

journal_title：Biochemical and biophysical research communications

authors： Xia H,Li B,Zhang Z,Wang Q,Qiao T,Li K

更新日期：2015-09-25 00:00:00

abstract：:Gene expression is the typical biological end point of interest following transfection. However, transcription may not accurately assess DNA uptake, or the ability of transfected DNA to be acted on by other enzymatic pathways. We have compared DNA uptake to gene expression and the unrelated enzymatic process of DNA do...

journal_title：Biochemical and biophysical research communications

authors： Pastwa E,Lubner EM,Mezhevaya K,Neumann RD,Winters TA

更新日期：2003-06-27 00:00:00

abstract：:Aggregation of the high-affinity IgE receptor (FcepsilonRI) activates a tyrosine phosphorylation cascade needed for enhanced adhesion and degranulation events on mast cells. We previously identified the adaptor ADAP (otherwise known as FYB/SLAP) as a modulator of integrin-mediated adhesion of T-cells and mast cells. H...

journal_title：Biochemical and biophysical research communications

authors： Geng L,Rudd C

更新日期：2001-12-21 00:00:00

abstract：:Plasmodium vivax malaria is one of the most prevalent parasitic diseases in Asia and Latin-America. The difficulty of maintaining this parasite culture in vitro has hampered identifying and characterising proteins implied in merozoite invasion of red blood cells. We have been able to identify an open reading frame in ...

journal_title：Biochemical and biophysical research communications

authors： Perez-Leal O,Sierra AY,Barrero CA,Moncada C,Martinez P,Cortes J,Lopez Y,Salazar LM,Hoebeke J,Patarroyo MA

更新日期：2005-06-17 00:00:00

abstract：:The crypt-villi axis of intestinal mucosa maintains homeostasis by renewal of epithelia, and also exhibits different properties from undifferentiated to terminally differentiated cells. We investigated differential susceptibility to genotoxin-induced cell death, based on the degree of differentiation of epithelial cel...

journal_title：Biochemical and biophysical research communications

authors： Chae B,Yang KM,Kim TI,Kim WH

更新日期：2009-01-23 00:00:00

abstract：:The prion diseases are neurodegenerative disorders characterized by the conversion of the PrPc (normal cellular prion) to the PrPsc (misfolded isoform). The accumulation of PrPsc within the central nervous system (CNS) leads to neurocytotoxicity by increasing oxidative stress. In addition, many neurodegenerative disor...

journal_title：Biochemical and biophysical research communications

authors： Jeong JK,Seol JW,Moon MH,Seo JS,Lee YJ,Kim JS,Park SY

更新日期：2010-10-29 00:00:00

abstract：:1,25(OH)2D3 receptors were studied in whole testes, Sertoli cells, seminiferous tubules, Leydig cells and spermatogonia of adult NMRI mice and SD rats. Specific reversible high affinity binding (KD 1.4 x 10(-10)M; Nmax 72 fmol/mg protein) by a 3.5 S macromolecule was demonstrated in whole testes, Sertoli cells and sem...

journal_title：Biochemical and biophysical research communications

authors： Merke J,Hügel U,Ritz E

更新日期：1985-02-28 00:00:00

abstract：:Neuromedin K and neuromedin L are novel mammalian tachykinins isolated from porcine spinal cord. We have developed a highly sensitive radioimmunoassay for neuromedin K. Since the radioimmunoassay for neuromedin K has significant crossreactivity with neuromedin L and substance P, we can simultaneously determine the tis...

journal_title：Biochemical and biophysical research communications

authors： Minamino N,Masuda H,Kangawa K,Matsuo H

更新日期：1984-11-14 00:00:00

abstract：:Cancer cells usually utilize glucose as a carbon source for aerobic glycolysis, a phenomenon known as the Warburg effect. Increasing studies have shown that PVT1 (a long non-coding RNA, lncRNA) functions as critical regulator of multiple cancers. However, it remains totally unknown whether and how PVT1 regulates gluco...

journal_title：Biochemical and biophysical research communications

authors： Song J,Wu X,Liu F,Li M,Sun Y,Wang Y,Wang C,Zhu K,Jia X,Wang B,Ma X

更新日期：2017-08-19 00:00:00

abstract：:Using the whole-cell and single channel recording techniques, the influence of actin cytoskeletons on L-type Ca2+ current was investigated in human gastric smooth muscle cells. In isotonic condition, an actin depolymerizer cytochalasin D (Cyt-D) markedly decreased the whole-cell current (I(Ba)) without changing steady...

journal_title：Biochemical and biophysical research communications

authors： Kim JH,Rhee PL,Kang TM

更新日期：2007-01-12 00:00:00

abstract：:The most widely used rate expression for single-substrate enzyme catalyzed reactions, namely the Michaelis-Menten kinetics is based upon the assumption that enzyme concentration is in excess of the substrate in the medium and the rate is mainly limited by the substrate concentration according to saturation kinetics. H...

journal_title：Biochemical and biophysical research communications

authors： Kargi F

更新日期：2009-04-24 00:00:00

abstract：:We cloned two novel types of TEA domain-containing transcription factor (ETFR-1 and -2) cDNAs. Amino acid sequences deduced for ETFR-1 and -2 as well as those of other known proteins of the same family, TEF-1 and ETF, exhibited significant identity (63-75% overall) to each other not only in the TEA DNA binding domain ...

journal_title：Biochemical and biophysical research communications

authors： Yasunami M,Suzuki K,Ohkubo H

更新日期：1996-11-12 00:00:00

abstract：:To evaluate the immunomodulatory effects of histamine in vivo, we analyzed an experimental syngenic tumor model using a colon adenocarcinoma cell line, CT-26, in Balb/c mice. In this model, distinct tumor growth was observed around 6 days after inoculation. Daily administration of cimetidine (0.12 mg/kg/day) significa...

journal_title：Biochemical and biophysical research communications

authors： Takahashi K,Tanaka S,Ichikawa A

更新日期：2001-03-01 00:00:00

abstract：:During IFN alpha stimulation, p59(fyn) associates with the Type I IFNR-associated Tyk-2 kinase in several human hematopoietic cell lines in vivo. This interaction is direct, and is mediated by the SH2 domain in p59(fyn), as shown by binding studies using glutathione-S-transferase fusion proteins and far western blots....

journal_title：Biochemical and biophysical research communications

authors： Uddin S,Sher DA,Alsayed Y,Pons S,Colamonici OR,Fish EN,White MF,Platanias LC

更新日期：1997-06-09 00:00:00

abstract：:The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right...

journal_title：Biochemical and biophysical research communications

authors： D'Urso A,Choi JK,Shabbir-Hussain M,Ngwa FN,Lambousis MI,Purrello R,Balaz M

更新日期：2010-06-25 00:00:00

abstract：:In this study, we devised a simple and rapid method to analyze fidelity of reverse transcriptase (RT) using next-generation sequencing (NGS). The method comprises a cDNA synthesis reaction from standard RNA with a primer containing a tag of 14 randomized bases and the RT to be tested, PCR using high-fidelity DNA polym...

journal_title：Biochemical and biophysical research communications

authors： Yasukawa K,Iida K,Okano H,Hidese R,Baba M,Yanagihara I,Kojima K,Takita T,Fujiwara S

更新日期：2017-10-14 00:00:00

abstract：:3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.3.4.) activity and cell membrane fluidity measured by fluorescence polarization using 1,6 diphenyl, 1,3,5-hexatriene as fluorescent probe have been concomitantly examined in HTC hepatoma cells, both in relation to growth rate and in response to treatment with h...

journal_title：Biochemical and biophysical research communications

authors： Richert L,Castagna M,Beck JP,Rong S,Luu B,Ourisson G

更新日期：1984-04-16 00:00:00

abstract：:Muscarinic receptor extracted from porcine atria in digitonin-cholate copurified with Galpha(o), Galpha(i1-3), and caveolins. The presence of complexes was confirmed by coimmunoprecipitation of the receptor, alpha-subunits, and caveolins in various combinations. Homooligomers of alpha(i2) were detected on Western blot...

journal_title：Biochemical and biophysical research communications

authors： Ma AW,Pawagi AB,Wells JW

更新日期：2008-09-12 00:00:00

abstract：:The majority of studies on possible roles for collagen hydrolysates in human health have focused on their effects on bone and skin. Hydroxyprolyl-glycine (Hyp-Gly) was recently identified as a novel collagen hydrolysate-derived dipeptide in human blood. However, any possible health benefits of Hyp-Gly remain unclear. ...

journal_title：Biochemical and biophysical research communications

authors： Kitakaze T,Sakamoto T,Kitano T,Inoue N,Sugihara F,Harada N,Yamaji R

更新日期：2016-09-23 00:00:00

abstract：:The ability of phospholipase C inhibitors to inhibit Ca2+ channel stimulation and Ca2+ release from intracellular stores evoked by norepinephrine in single rat portal vein myocytes was investigated in the aim of identifying the type of phospholipase C involved in the transduction pathways activated by alpha 1A- and al...

journal_title：Biochemical and biophysical research communications

authors： Macrez-Leprêtre N,Morel JL,Mironneau J

更新日期：1996-01-05 00:00:00

abstract：:We have isolated a rabbit metallothionein-I gene from a lambda gt10 library. The coding sequence of this gene is interrupted by two introns occurring at amino acid positions 9 1/3 and 30 1/3. Comparison of the promoter sequence of this gene with the promoters of other metallothionein genes identified a number of oligo...

journal_title：Biochemical and biophysical research communications

authors： Tam YC,Chopra A,Hassan M,Thirion JP

更新日期：1988-05-31 00:00:00

abstract：:In order to investigate the utility of intranasal administration of peptides for systemic medication, the nasal absorption of the model peptide, leucine enkephalin (Tyr-Gly-Gly-Phe-Leu), was studied in the rat. At a concentration of 60 micrograms/ml in Ringer's buffer the pentapeptide was found to undergo, extensive h...

journal_title：Biochemical and biophysical research communications

authors： Hussain A,Faraj J,Aramaki Y,Truelove JE

更新日期：1985-12-31 00:00:00

abstract：:CCG-1423, a chemical inhibitor of Rho signaling, blocks serum response factor (SRF)/megakaryoblastic leukemia 1 (MKL1)-mediated gene expression by inhibiting the nuclear accumulation of MKL1. Several studies have suggested that CCG-1423 interacts not only with MKL1, which has a critical role in the regulation of neuro...

journal_title：Biochemical and biophysical research communications

authors： Kikuchi K,Shiota J,Yamada T,Ishikawa M,Ihara D,Fukuchi M,Tsuda M,Tabuchi A

更新日期：2017-10-21 00:00:00

abstract：:Vaspin, an adipocytokine recently identified in a rat model of type 2 diabetes, has been suggested to have an insulin-sensitizing effect. However, the exact mechanism underlying this action has not been fully elucidated. Furthermore, the specific function of vaspin is largely unknown, especially in vascular cells. We ...

journal_title：Biochemical and biophysical research communications

authors： Jung CH,Lee WJ,Hwang JY,Seol SM,Kim YM,Lee YL,Park JY

更新日期：2011-09-23 00:00:00

abstract：:Male Djungarian hamsters (Phodopus sungorus; 45 animals per group) were either sham-exposed or exposed to a sinusoidal magnetic field for 56 days (Experiment 1: 50 Hz, 450 microTesla peak; max. dB/dt = 140 mTesla s(-1); 24 hrs day(-1)). Except for day 7, no effects were observed with respect to body weights during exp...

journal_title：Biochemical and biophysical research communications

authors： Niehaus M,Brüggemeyer H,Behre HM,Lerchl A

更新日期：1997-05-29 00:00:00