DNA cleavage characteristics of non-protein enediyne antibiotic N1999A2.

abstract：:Helodermin is a peptide from the venom of the lizard Heloderma suspectum (Gila Monster) showing a high degree of sequence similarity with VIP, PHI and secretin in its N-terminal moiety. The present data support the presence of peptide(s) closely related to helodermin in the brain, gut and salivary glands of rat. In ou...

journal_title：Biochemical and biophysical research communications

authors： Robberecht P,De Graef J,Woussen MC,Vandermeers-Piret MC,Vandermeers A,De Neef P,Cauvin A,Yanaihara C,Yanaihara N,Christophe J

更新日期：1985-07-16 00:00:00

abstract：OBJECTIVE:Inflammatory bowel diseases (IBD) including ulcerative colitis and Crohn's disease are devastating diseases of the gut. At present, all the treatments are mainly targeting symptoms like inflammation. The disease remains regarded as incurable, largely due to lacking of knowledge on its etiology. Our previous s...

journal_title：Biochemical and biophysical research communications

authors： He Y,Yu H,Ge Y,Li X,Jiang M,Liu Y,Li X,Wang Y,Guo M,Qin X,Wang X

更新日期：2019-05-28 00:00:00

abstract：:Three corticostatic peptides (GP-CS1, GP-CS2 and GP-CS3) were purified from extracts of guinea pig bone marrow. Each was identified on the basis of their ability to inhibit the secretion of corticosterone by isolated rat adrenal cells stimulated by ACTH. GP-CS1 and GP-CS2 were found to be 31 residues in length, rich i...

journal_title：Biochemical and biophysical research communications

authors： Hu J,Bennett HP,Lazure C,Solomon S

更新日期：1991-10-31 00:00:00

abstract：:1-4 interaction scaling factors are used in AMBER forcefields to reduce the exaggeration of short-range repulsion caused by the 6-12 Lennard-Jones potential and a nonpolarizable charge model and to obtain better agreements of small-molecule conformational energies with experimental data. However, the effects of these ...

journal_title：Biochemical and biophysical research communications

authors： Pang YP

更新日期：2015-02-06 00:00:00

abstract：:Wound healing in mammals can take several weeks to months and the process is always accompanied by scar formation. Wound healing mechanisms that mimic regeneration are not found in most mature mammalian tissues. However, the MRL/MPJ (MRL) mouse has the unique capacity to regenerate ear hole wound completely in less th...

journal_title：Biochemical and biophysical research communications

authors： Masinde G,Li X,Baylink DJ,Nguyen B,Mohan S

更新日期：2005-04-29 00:00:00

abstract：:To further define the molecular interaction between decorin and type I collagen we generated a 20 kD fragment containing the N-terminal half of the core protein by Endoproteinase Arg C digestion and a 40 kD fragment including all leucine-rich repeats in the central part of decorin core by cleavage with 2-nitro-5-thioc...

journal_title：Biochemical and biophysical research communications

authors： Pogány G,Vogel KG

更新日期：1992-11-30 00:00:00

abstract：:TESK1 (testis-specific protein kinase 1) is a serine/threonine kinase, with a unique structure composed of an N-terminal protein kinase domain and a C-terminal proline-rich domain. Northern blot analysis revealed that TESK1 mRNA is predominantly expressed in testicular germ cells. We present here evidence that express...

journal_title：Biochemical and biophysical research communications

authors： Toshima J,Koji T,Mizuno K

更新日期：1998-08-10 00:00:00

abstract：:p202, an interferon (IFN) inducible protein, is a phosphonuclear protein involved in the regulation of cell cycle, apoptosis, and differentiation. E2F1 belongs to the E2F family of proteins that are important cell cycle regulators in promoting cell growth. On the other hand, the deregulated expression of E2F1 also tri...

journal_title：Biochemical and biophysical research communications

authors： Yan DH,Abramian A,Li Z,Ding Y,Wen Y,Liu TJ,Hunt K

更新日期：2003-03-28 00:00:00

abstract：:Lateral diffusion coefficients (D) of two surface differentiation antigens (sIgM and Bp35) were determined on interferon-sensitive (-IFs) or resistant (-IFr) Daudi cells by fluorescence photobleaching, using monospecific FITC-anti-IgM or PE-anti-Leu 16 probes. For untreated Daudi -IFs, mean (D) were 5.8 and 5.3 (x10(-...

journal_title：Biochemical and biophysical research communications

authors： Balint E,Aszalos A,Grimley PM

更新日期：1988-12-15 00:00:00

abstract：:The CD133 antigen, identified as a hematopoietic stem cell marker, appears in various human embryonic epithelia including the neural tube, gut, and kidney. We herein investigated whether CD133(+) cells isolated from human hepatocellular carcinoma cell lines possess cancer stem/progenitor cell-like properties. Among th...

journal_title：Biochemical and biophysical research communications

authors： Suetsugu A,Nagaki M,Aoki H,Motohashi T,Kunisada T,Moriwaki H

更新日期：2006-12-29 00:00:00

abstract：:microRNA-222 (miR-222) has been shown to initiate the activation of hepatic stellate cells, which plays an important role in the pathogenesis of liver fibrosis. The aim of our study was to evaluate the role of miR-22 in a mouse model of biliary atresia (BA) induced by Rhesus Rotavirus (RRV) infection. New-born Balb/c ...

journal_title：Biochemical and biophysical research communications

authors： Shen WJ,Dong R,Chen G,Zheng S

更新日期：2014-03-28 00:00:00

abstract：:TGFbeta is of crucial importance during transdifferentiation of resting retinoid-storing hepatic stellate cells (HSC) to extracellular matrix producing myofibroblasts (MFB) and consequently, inhibition of TGFbeta signal transduction is an effective means for preventing experimental fibrosis. We have shown that isolate...

journal_title：Biochemical and biophysical research communications

authors： Dooley S,Streckert M,Delvoux B,Gressner AM

更新日期：2001-05-11 00:00:00

abstract：:C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor involved in cell migration, apoptosis, and tumorization. Up-regulation of CtBP2 is implicated in various human cancers including breast cancers. In our present study, we found that the C-terminus of Hsc70-interacting protein (CHIP) regulated the st...

journal_title：Biochemical and biophysical research communications

authors： Lee JS,Yoo SJ

更新日期：2013-03-15 00:00:00

abstract：:Calumenin is a multiple EF-hand Ca2+-binding protein located in endo/sarcoplasmic reticulum of mammalian tissues. In the present study, we cloned two rabbit calumenin isoforms (rabbit calumenin-1 and -2, GenBank Accession Nos. SY225335 and AY225336, respectively) by RT-PCR. Both isoforms contain a 19 aa N-terminal sig...

journal_title：Biochemical and biophysical research communications

authors： Jung DH,Mo SH,Kim DH

更新日期：2006-04-28 00:00:00

abstract：:A method for the extraction and purification of PrP(C), in its native monomeric form, from outdated human platelet concentrates is described. Both calcium ionophore platelet activation and lysis in Triton X-100 were evaluated as methods for the extraction of soluble platelet PrP(C) in its monomeric form. Following pla...

journal_title：Biochemical and biophysical research communications

authors： Jones M,Head MW,Connolly JG,Farquhar CF,Hornsey VS,Pepper DS,MacGregor IR

更新日期：2005-09-16 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the transforming growth factor-beta superfamily. It has been demonstrated that BMPs had been involved in the regulation of cell proliferation, survival, differentiation and apoptosis. However, their hallmark ability is that play a pivo...

journal_title：Biochemical and biophysical research communications

authors： Xiao YT,Xiang LX,Shao JZ

更新日期：2007-10-26 00:00:00

abstract：:A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library. This clone contains a 1521 bp open reading frame coding for a 506 amino acid residue protein which has a 30 amino acid extension peptide which is 6 resi...

journal_title：Biochemical and biophysical research communications

authors： Zhou M,Gomez-Sanchez CE

更新日期：1993-07-15 00:00:00

abstract：:Stromal interaction molecule 1 (STIM1) is an endoplasmic reticulum (ER)-membrane associated Ca(2+) sensor which activates store-operated Ca(2+) entry (SOCE). The homologue, STIM2 possesses a high sequence identity to STIM1 ( approximately 61%), while its role in SOCE seems to be distinct from that of STIM1. In order t...

journal_title：Biochemical and biophysical research communications

authors： Zheng L,Stathopulos PB,Li GY,Ikura M

更新日期：2008-04-25 00:00:00

abstract：:To simultaneously assess the effects of exonic mutations on RNA splicing and protein functions, we report here an intron-inclusive cDNA (Intinc) expression system. As a test model, twenty-four mutations in exon 9 of the phenylalanine hydroxylase (PAH) gene were examined in an Intinc expression plasmid composed of the ...

journal_title：Biochemical and biophysical research communications

authors： Ho PY,Huang MZ,Fwu VT,Lin SC,Hsiao KJ,Su TS

更新日期：2008-09-05 00:00:00

abstract：:In a simple salts medium, monolayers of IEC-6 intestinal cells achieved concentrations of unmetabolized formycin B (an analog of inosine) about 6-fold higher than in the medium. Rates of formycin B influx were a saturable function of Na+ concentrations in the medium. Although IEC-6 cells possess sites with high affini...

journal_title：Biochemical and biophysical research communications

authors： Jakobs ES,Paterson AR

更新日期：1986-11-14 00:00:00

abstract：:Genomic DNA of the phytopathogenic Erwinia carotovora subsp. carotovora LY34 was partially digested with Sau3AI, ligated into the BamHI site of pBlue-script II SK+, and introduced into E. coli. Two clones that were able to hydrolyse carboxymethylcellulose were selected. 1.5 kb and 1.2 kb fragments containing the celA ...

journal_title：Biochemical and biophysical research communications

authors： Park YW,Lim ST,Cho SJ,Yun HD

更新日期：1997-12-29 00:00:00

abstract：:Human follitropin receptor (hFSHR) cDNA was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter. Spodoptera frugiperda (Sf9) insect cells expressed the hFSHR at the membrane level, 2-3 days after infection. Ligand binding analysis demonstrated satu...

journal_title：Biochemical and biophysical research communications

authors： Christophe S,Robert P,Maugain S,Bellet D,Koman A,Bidart JM

更新日期：1993-10-15 00:00:00

abstract：:During the search for ANP receptor ligands of microbial origin, we isolated a novel polysaccharide, HS-142-1, from culture broth of Aureobasidium sp. HS-142-1 inhibited [125I]-rANP binding to ANP receptor in rabbit kidney cortex membranes with an IC50 of 0.3 mu g/ml, but gave no effects on specific binding of [125I]-E...

journal_title：Biochemical and biophysical research communications

authors： Morishita Y,Sano T,Ando K,Saitoh Y,Kase H,Yamada K,Matsuda Y

更新日期：1991-05-15 00:00:00

abstract：:Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on ce...

journal_title：Biochemical and biophysical research communications

authors： Sakamoto H,Zhang XQ,Suenobu S,Ohbo K,Ogawa M,Suda T

更新日期：2004-08-27 00:00:00

abstract：:The number of accessible SH-groups was determined in membrane vesicles prepared from Escherichia coli growing in fermentation conditions at slightly alkaline pH on glucose with or without added formate. Addition of ATP or formate to the vesicles caused a approximately 1.4-fold increase in the number of accessible SH-g...

journal_title：Biochemical and biophysical research communications

authors： Mnatsakanyan N,Poladian A,Bagramyan K,Trchounian A

更新日期：2003-08-29 00:00:00

abstract：:The production of nitric oxide (NO.) and the induction of glucose-6-phosphate dehydrogenase by lipopolysaccharides (LPS) from different sources was studied in bone marrow derived macrophages (BMM phi). NO. production was found to be linked to the induction of glucose-6-phosphate dehydrogenase, suggesting the possible ...

journal_title：Biochemical and biophysical research communications

authors： Corraliza IM,Campo ML,Fuentes JM,Campos-Portuguez S,Soler G

更新日期：1993-10-15 00:00:00

abstract：:Glutamate-gated chloride (GluCl) channels are pentameric receptors for the inhibitory neurotransmitter glutamate in invertebrates and are a major target for macrolide anthelmintics. Three amino acids in GluCl channels are reported to render macrolide resistance in nematodes and insects. To examine whether these three ...

journal_title：Biochemical and biophysical research communications

authors： Yamaguchi M,Sawa Y,Matsuda K,Ozoe F,Ozoe Y

更新日期：2012-03-16 00:00:00

abstract：:We demonstrate for the first time that major structural changes are imposed on various phospholipid bilayers by diacylglycerol, a product of phosphatidylinositol metabolism. By 5 mole percent in phosphatidylethanolamine a lamellar to hexagonal transition starts that is complete at 10 mole percent. At 30 mole percent i...

journal_title：Biochemical and biophysical research communications

authors： Das S,Rand RP

更新日期：1984-10-30 00:00:00

abstract：:Antisense activity in living cells has been thought to occur via a mechanism involving both DNA-mediated hybridization arrest of target mRNA and RNase H-mediated mRNA digestion. Therefore an ideal antisense agent should be permeable to the cell and possess capacities (1) to form a thermally stable duplex in vivo with ...

journal_title：Biochemical and biophysical research communications

authors： Matsukura M,Okamoto T,Miike T,Sawai H,Shinozuka K

更新日期：2002-05-24 00:00:00

abstract：:We have reported that the principal neutralizing domain of V3 of the HIV-1 gp120 induces an antigen-specific activation apoptosis of responding effector CD4+ T lymphocytes, a phenomenon inhibited by RANTES, an agonist of CCR5. Here, addressing the question of how a hypervariable region could induce such a selective re...

journal_title：Biochemical and biophysical research communications

authors： Baritaki S,Zafiropoulos A,Sioumpara M,Politis M,Spandidos DA,Krambovitis E

更新日期：2002-11-08 00:00:00