Sodium-dependent, concentrative nucleoside transport in cultured intestinal epithelial cells.

Abstract:

:In a simple salts medium, monolayers of IEC-6 intestinal cells achieved concentrations of unmetabolized formycin B (an analog of inosine) about 6-fold higher than in the medium. Rates of formycin B influx were a saturable function of Na+ concentrations in the medium. Although IEC-6 cells possess sites with high affinity for nitrobenzylthioinosine, a potent inhibitor of equilibrative (facilitated diffusion) nucleoside transport systems in certain cell types, the inhibitor had only minor effects on formycin B uptake in IEC-6 cells, but reduced efflux of the analog from these cells. These findings indicate the joint presence in IEC-6 cells of nucleoside transporters of two types, one that is concentrative and Na+-dependent, and another that is sensitive to nitrobenzylthioinosine and apparently equilibrative.

authors

Jakobs ES,Paterson AR

doi

10.1016/0006-291x(86)90738-2

subject

Has Abstract

pub_date

1986-11-14 00:00:00

pages

1028-35

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(86)90738-2

journal_volume

140

pub_type

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