The number of accessible SH-groups in Escherichia coli membrane vesicles is increased by ATP or by formate.

abstract：:We initiated the present work as part of an effort to identify and characterize genes from the EST2-HMG14 region from human chromosome 21 potentially responsible for some of the Down syndrome (DS) features. Genomic sample sequencing with cosmid clone A1047 located in the ETS2-HMG14 region of chromosome 21 has led to t...

journal_title：Biochemical and biophysical research communications

authors： Vidal-Taboada JM,Sanz S,Egeo A,Scartezzini P,Oliva R

更新日期：1998-09-29 00:00:00

abstract：:SPE-16 is a new 16kDa protein that has been purified from the seeds of Pachyrrhizus erosus. It's N-terminal amino acid sequence shows significant sequence homology to pathogenesis-related class 10 proteins. cDNA encoding 150 amino acids was cloned by RT-PCR and the gene sequence proved SPE-16 to be a new member of PR-...

journal_title：Biochemical and biophysical research communications

authors： Wu F,Yan M,Li Y,Chang S,Song X,Zhou Z,Gong W

更新日期：2003-12-19 00:00:00

abstract：:Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerp...

journal_title：Biochemical and biophysical research communications

authors： Hozumi A,Satouh Y,Ishibe D,Kaizu M,Konno A,Ushimaru Y,Toda T,Inaba K

更新日期：2004-07-09 00:00:00

abstract：:Polymerization of actin induced by activation of platelets was investigated using deoxyribonuclease I inhibition assay. When platelets were activated with ADP or 5-hydroxytryptamine, actin was polymerized quickly followed by rapid depolymerization to the initial level. Reactivation with the same agonist, however, did ...

journal_title：Biochemical and biophysical research communications

authors： Imada T,Kikuchi T,Shimada H,Inada Y,Saito Y

更新日期：1984-05-16 00:00:00

abstract：:To investigate the biological function of CKII, we have identified proteins that interact with the subunits of CKII using the yeast two-hybrid system. Here we report that SAG, an antioxidant protein containing Ring-H2 finger motif, is a cellular partner associating with the beta subunit of CKII. SAG does not interact ...

journal_title：Biochemical and biophysical research communications

authors： Son MY,Park JW,Kim YS,Kang SW,Marshak DR,Park W,Bae YS

更新日期：1999-10-05 00:00:00

abstract：:We have investigated the effect of chloroform on the phosphorylation of myelin basic proteins because tumor-promoting agents such as phorbol esters and chloroform are known to enhance the activity of protein kinase C. We report that the presence of chloroform, at a concentration known to enhance protein kinase C activ...

journal_title：Biochemical and biophysical research communications

authors： Ulmer JB,Braun PE

更新日期：1987-08-14 00:00:00

abstract：:To repress gene transcription, the unliganded nuclear receptor (NR) recruits the N-CoR and SMRT corepressors via its direct association with the conserved motif within bipartite NR-interaction domains (IDs) of corepressors. We recently reported that SMRT is directly involved in the VDR-mediated repression via an ID1-s...

journal_title：Biochemical and biophysical research communications

authors： Kim JY,Son YL,Lee YC

更新日期：2009-02-13 00:00:00

abstract：:Partly purified 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoyl-CoA oxidase from rabbit liver peroxisomes was found to convert the 25S- but not the 25R diastereoisomer of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-27-oyl-CoA into (24E)-3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholest-24-en-27...

journal_title：Biochemical and biophysical research communications

authors： Pedersen JI,Veggan T,Björkhem I

更新日期：1996-07-05 00:00:00

abstract：:Transfer of the gene for L-gulono-gamma-lactone oxidase, the missing enzyme in L-ascorbic acid biosynthesis in scurvy-prone animals, into medaka (Oryzias latipes) was successfully done. The expression plasmid pSVL-GLO, carrying rat liver L-gulono-gamma-lactone oxidase cDNA, was microinjected into the cytoplasm of fert...

journal_title：Biochemical and biophysical research communications

authors： Toyohara H,Nakata T,Touhata K,Hashimoto H,Kinoshita M,Sakaguchi M,Nishikimi M,Yagi K,Wakamatsu Y,Ozato K

更新日期：1996-06-25 00:00:00

abstract：:Steatosis is an important clinical manifestation of hepatitis C virus (HCV) infection. The molecular mechanisms of HCV-associated steatosis are not well understood. Sterol regulatory element-binding protein-1c (SREBP-1c) is a key transcription factor which activates the transcription of lipogenic genes. Here we showed...

journal_title：Biochemical and biophysical research communications

authors： Xiang Z,Qiao L,Zhou Y,Babiuk LA,Liu Q

更新日期：2010-11-19 00:00:00

abstract：:The role of mycothiol in mycobacteria was examined by comparative analysis of mutants disrupted in the four known genes encoding the protein machinery needed for mycothiol biosynthesis. These mutants were sensitive to acid stress, antibiotic stress, alkylating stress, and oxidative stress indicating that mycothiol and...

journal_title：Biochemical and biophysical research communications

authors： Rawat M,Johnson C,Cadiz V,Av-Gay Y

更新日期：2007-11-09 00:00:00

abstract：:Fatty acid biosynthesis is crucial for all living cells. In contrast to higher organisms, bacteria use a type II fatty acid synthase (FAS II) composed of a series of individual proteins, making FAS II enzymes excellent targets for antibiotics discovery. The beta-hydroxyacyl-ACP dehydratase (FabZ) catalyzes an essentia...

journal_title：Biochemical and biophysical research communications

authors： Kirkpatrick AS,Yokoyama T,Choi KJ,Yeo HJ

更新日期：2009-03-06 00:00:00

abstract：:NADH-rubredoxin oxidoreductase (NROR), a flavoprotein from the obligately anaerobe Clostridium acetobutylicum is encoded by an ORF (nror) of 1140 nucleotides. Whereas primary structure analysis reveals that NROR has amino acid sequence patterns homologous with those involved in FAD and NAD-binding, the enzyme is dista...

journal_title：Biochemical and biophysical research communications

authors： Guedon E,Petitdemange H

更新日期：2001-07-13 00:00:00

abstract：:Mammary gland-distributed and ER-bound UDP-glucuronosyltransferase (UGT)-2B7 metabolizes genotoxic catechol-estrogens (CE) associated with breast cancer initiation. Although UGT2B7 has 3 PKC- and 2 tyrosine kinase (TK)-sites, its inhibition by genistein, herbimycin-A and PP2 with parallel losses in phospho-tyrosine an...

journal_title：Biochemical and biophysical research communications

authors： Mitra PS,Basu NK,Owens IS

更新日期：2009-05-15 00:00:00

abstract：:Tumor-induced lymphangiogenesis, a major conduit for cancer cell dissemination from the primary tumor site to lymph nodes and beyond, eventually leads to metastasis in cancer patients. Given the recent evidence revealing that the suppression of ELK3 inhibits the metastasis of triple-negative breast cancer cells, we ai...

journal_title：Biochemical and biophysical research communications

authors： Oh N,Park JI,Park JH,Kim KS,Lee DR,Park KS

更新日期：2017-03-18 00:00:00

abstract：:Various stresses including ionizing radiation give normal human fibroblasts a phenotype of senescence-like growth arrest (SLGA), manifested by p53-dependent irreversible G1 arrest. To determine the mechanism of persistent activation of p53, we examined phosphorylated Ataxia telangiectasia mutated (ATM) and phosphoryla...

journal_title：Biochemical and biophysical research communications

authors： Suzuki M,Suzuki K,Kodama S,Watanabe M

更新日期：2006-02-03 00:00:00

abstract：:Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectabl...

journal_title：Biochemical and biophysical research communications

authors： Yokosuka O,Ito Y,Imazeki F,Ohto M,Omata M

更新日期：1992-11-30 00:00:00

abstract：:Mouse hepatocytes were found to adhere much stronger to intact native type I collagen than to native, telopeptide-devoid collagen. Removal of telopeptides (by pepsin treatment) caused disintegration of supramolecular collagen aggregates. Similar differences in hepatocyte adhesion were observed between oligomeric and m...

journal_title：Biochemical and biophysical research communications

authors： Berman A,Morozevich G,Karmansky I,Gleiberman A,Bychkova V

更新日期：1993-07-15 00:00:00

abstract：:Reactive oxygen species (ROS) are a continuous hazard in eukaroytic cells by their ability to cause damage to biomolecules, in particular to DNA. Previous data indicated that the cytosolic serine peptidase tripeptidyl-peptidase II (TPPII) translocates into the nucleus of most tumor cell lines in response to gamma-irra...

journal_title：Biochemical and biophysical research communications

authors： Preta G,de Klark R,Chakraborti S,Glas R

更新日期：2010-08-27 00:00:00

abstract：:Triacsin C, a product of Streptmyces sp. SK-1894, was previously reported as an inhibitor of long chain acyl-CoA synthetase. Pretreatment with triacsin C (500 nM) for 1h enhanced production of platelet-activating factor in rat neutrophils, followed by stimulation with A23187 or fMLP. Amount of lyso-PAF was also augume...

journal_title：Biochemical and biophysical research communications

authors： Hayashi M,Imai Y,Naraba H,Tomoda H,Omura S,Oh-ishi S

更新日期：1992-11-16 00:00:00

abstract：:Glycoprotein IIIb (GPIV, CD36) has been proposed as the platelet receptor for thrombospondin (TSP). We found two healthy blood donors, whose platelets were shown to be GPIIIb deficient. These platelets expressed endogeneous TSP as control platelets and their binding capacity for exogeneous TSP was the same. These resu...

journal_title：Biochemical and biophysical research communications

authors： Kehrel B,Kronenberg A,Schwippert B,Niesing-Bresch D,Niehues U,Tschöpe D,van de Loo J,Clemetson KJ

更新日期：1991-09-16 00:00:00

abstract：:Extracellular calcium addition transiently stimulated two S6 peptide kinase activities in isolated rat hepatocytes. Mono Q chromatography revealed that the activities eluting at 0.15 M NaCl and 0.18 M NaCl were stimulated 4-fold and 2-fold, respectively. The kinase stimulated by calcium was a 40000-Mr S6 peptide kinas...

journal_title：Biochemical and biophysical research communications

authors： Harlin JC,Tredano M,Rider MH,Bollen M,Chédeville A,Lavoinne A

更新日期：1997-10-20 00:00:00

abstract：:Interleukin-1 alpha (IL-1 alpha) and interleukin-6 (IL-6), both known to be able to open the blood-brain barrier (BBB), downregulated plasma membrane-associated tyrosine phosphatase activity in primary porcine brain endothelial cells (PBEC). In contrast, transforming growth factor beta (TGF-beta) upregulated PTP activ...

journal_title：Biochemical and biophysical research communications

authors： Gloor SM,Weber A,Adachi N,Frei K

更新日期：1997-10-29 00:00:00

abstract：:In Chinese hamster ovary cells, [3H]formycin B is metabolized into formycin B-5'-monophosphate, formycin A-5'-monophosphate and higher phosphorylated derivatives of formycin A which are incorporated into RNA. Mutants of CHO cells independently selected for resistance to various adenosine analogs viz. toyocamycin, tube...

journal_title：Biochemical and biophysical research communications

authors： Mehta KD,Gupta RS

更新日期：1985-07-31 00:00:00

abstract：:The Rep proteins of some plasmid replicons have two functions. Dimers bind to the operator sequences acting as auto-repressors, whereas monomers bind to the iterons to initiate replication of DNA. The ColE2 Rep proteins are present mostly in a dimeric form with some multimers larger than dimers in solution, while the ...

journal_title：Biochemical and biophysical research communications

authors： Han M,Aoki K,Yagura M,Itoh T

更新日期：2007-02-09 00:00:00

abstract：:We have isolated and characterized the 5'-flanking region of the mouse RECK gene aiming to understand the mechanism of oncogene-mediated suppression of RECK gene expression. The upstream 52-base region was found to contain a promoter activity which is, to some extent, suppressed by the ras oncogene. This region contai...

journal_title：Biochemical and biophysical research communications

authors： Sasahara RM,Takahashi C,Noda M

更新日期：1999-11-02 00:00:00

abstract：:Treatment of newborn murine epidermal cells with phospholipase C results in the generation of a chemiluminescence response similar to that previously described for phorbol ester tumor promoters. Based on inhibitor studies, the oxidant, believed to be superoxide anion, is most likely generated from lipoxygenase metabol...

journal_title：Biochemical and biophysical research communications

authors： Fischer SM,Baldwin JK,Adams LM

更新日期：1985-09-30 00:00:00

abstract：:To investigate whether residues in the extracellular domains of melanocortin 1 receptor (MC1R) are required for ligand binding, a number of mutants were constructed where charged residues were converted to alanine. The residues targeted for mutagenesis were Ser6, Glu102, Arg109, Asp184, Glu269, and Thr272. The mutant ...

journal_title：Biochemical and biophysical research communications

authors： Chhajlani V,Xu X,Blauw J,Sudarshi S

更新日期：1996-02-15 00:00:00

abstract：:ADAM17 is known to contribute to the immune system through its shedding of tumor necrosis factor alpha (TNFα). However, the role of ADAM17 in B cell biology is not well characterized. To determine whether B cell ADAM17 contributes to T cell-independent humoral immune responses, we crossed CD19 Cre transgenic mice with...

journal_title：Biochemical and biophysical research communications

authors： Lownik JC,Wimberly JL,Takahashi-Ruiz L,Martin RK

更新日期：2020-02-05 00:00:00

abstract：:Erythropoietin has been thought to be secreted to plasma soon after the production because of the difficulty of Western blot analysis and immunohistochemistry. We established the new methods of Western blot analysis and immunohistochemistry. Using the new methods, we investigated the effects of aldosterone and fludroc...

journal_title：Biochemical and biophysical research communications

authors： Yasuoka Y,Izumi Y,Nagai T,Fukuyama T,Nakayama Y,Inoue H,Horikawa K,Kimura M,Nanami M,Yanagita K,Oshima T,Yamazaki T,Uematsu T,Yamamura R,Kobayashi N,Shimada Y,Nagaba Y,Nakanishi T,Yamashita T,Mukoyama M,Sato Y,K

更新日期：2018-09-18 00:00:00