Abstract:
:A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library. This clone contains a 1521 bp open reading frame coding for a 506 amino acid residue protein which has a 30 amino acid extension peptide which is 6 residues longer than that reported by others. Seven nucleotide mutations in the coding region of the mature protein were found in comparison to the reported cDNAs. These mutations resulted in three amino acid changes in the mature protein which were identical to those present in the cytochrome P-450 11 beta-hydroxylase (CYP11B1). Two nucleotide substitutions and two nucleotide insertions were also identified in the 3'-noncoding region. The expression product of this cDNA transfected into COS7 cells converted DOC to aldosterone, corticosterone and 18-hydroxycorticosterone less efficiently than the plasmid containing the cDNA with normal 24 amino acid extension peptide.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Zhou M,Gomez-Sanchez CEdoi
10.1006/bbrc.1993.1792subject
Has Abstractpub_date
1993-07-15 00:00:00pages
112-7issue
1eissn
0006-291Xissn
1090-2104pii
S0006291X83717924journal_volume
194pub_type
杂志文章abstract::The effect of platelet release products on cytosolic calcium [( Ca++]i) was examined by monitoring the fluorescence of chick embryonic heart cells loaded with the fluorescent calcium indicator indo-1 AM. Cell free filtrate of platelet release products was obtained from rabbit platelets activated with thrombin or colla...
journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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