Identification of a Bcl-XL binding region within the ATPase domain of Apaf-1.

abstract：:A single addition of ATP (20-1000 microM) to cultures of HL-60 cells resulted here in permanent, Ca(2+)-independent inhibition of cellular proliferation, evident 48 h following treatment. Extracellular ATP (ATPo) was maximally effective at 250 microM giving 90 +/- 1.5% growth inhibition. Up to a concentration of 250 m...

journal_title：Biochemical and biophysical research communications

authors： Seetulsingh-Goorah SP,Stewart BW

更新日期：1998-09-18 00:00:00

abstract：:Activation of reactive oxygen and nitrogen species (RONS) by redox-active metal ions has been proposed to contribute to oxidative damage in inflamed tissues. Here, we report a dual-function anti-oxidant conjugate comprising an anti-inflammatory agent (5-aminosalicylic acid) and a chelator with potential as a superoxid...

journal_title：Biochemical and biophysical research communications

authors： Bailey MA,Ingram MJ,Naughton DP

更新日期：2004-05-14 00:00:00

abstract：:We report that N-oleoylethanolamine (NOE), widely employed as a ceramidase inhibitor, also inhibits glucosylation of naturally occurring ceramides. When CHP-100 neuroepithelioma cells were exposed for 18h to non-toxic NOE concentrations (i.e. up to 70 microM), basal incorporation of labelled hexose into glucosylcerami...

journal_title：Biochemical and biophysical research communications

authors： Spinedi A,Di Bartolomeo S,Piacentini M

更新日期：1999-02-16 00:00:00

abstract：:Intravenous immunoglobulin (IVIG) is periodically administered to immunocompromised patients together with antimicrobial agents. The evidence that supports the effectiveness of IVIG is mostly based on data from randomized clinical trials; the underlying mechanisms are poorly understood. A recent study revealed that ki...

journal_title：Biochemical and biophysical research communications

authors： Matsuo H,Itoh H,Kitamura N,Kamikubo Y,Higuchi T,Shiga S,Ichiyama S,Kondo T,Takaori-Kondo A,Adachi S

更新日期：2015-08-14 00:00:00

abstract：:Human glutathione synthetase is responsible for catalyzing the final step in glutathione biosynthesis. It is a homodimer with a monomer subunit MW of 52 kDa. Kinetic analysis reveals a departure from linearity of the Lineweaver-Burk double reciprocal plot for the binding of gamma-glutamyl substrate, indicating coopera...

journal_title：Biochemical and biophysical research communications

authors： Njalsson R,Norgren S,Larsson A,Huang CS,Anderson ME,Luo JL

更新日期：2001-11-23 00:00:00

abstract：:GLAST and GLT-1 are the most abundant glutamate transporters in the CNS and protect neurons from glutamate neurotoxicity. Here, we investigated the role of GLAST in spinal nociceptive processing. GLAST protein expression was not altered after treatment of rats with either formalin or zymosan. Surprisingly, knock-down ...

journal_title：Biochemical and biophysical research communications

authors： Niederberger E,Schmidtko A,Coste O,Marian C,Ehnert C,Geisslinger G

更新日期：2006-07-28 00:00:00

abstract：:Protein phosphatase was partially purified from myofibrils of bovine heart by sequential column chromatographies. The purified protein phosphatase was immunologically identified as a delta isoform of PP1 (PP1delta). The myosin-binding subunit (MBS) of myosin-binding phosphatase (MBP) in smooth muscle was co-purified w...

journal_title：Biochemical and biophysical research communications

authors： Nishio H,Ichikawa K,Hartshorne DJ

更新日期：1997-07-30 00:00:00

abstract：:Within the first external loop of mouse and human TRESK subunits one or two N-glycosylation consensus sites were identified, respectively. Using site directed mutagenesis and Western immunoblotting a single residue of both orthologues was found to be glycosylated upon heterologous expression. Two-electrode voltage-cla...

journal_title：Biochemical and biophysical research communications

authors： Egenberger B,Polleichtner G,Wischmeyer E,Döring F

更新日期：2010-01-08 00:00:00

abstract：:A new protein observed in two-dimensional electrophoresis patterns of proteins from the human skin fibroblast line GM1386 has been identified as a charge and molecular-weight variant of the type of tropomyosin found in smooth muscle (Tm:3). This is the second variant of Tm:3 found in GM1386 and represents a second sit...

journal_title：Biochemical and biophysical research communications

authors： Giometti CS,Gemmell MA,Anderson NL

更新日期：1985-05-16 00:00:00

abstract：:Type 1 diabetes (T1D) is an autoimmune disease caused by destruction of insulin-producing β cells. The response of autoreactive T cells to β cell antigens plays a central role in the development of T1D. Recently, fusion peptides composed by insulin C-peptide fragments and other proteins were reported as β cell target ...

journal_title：Biochemical and biophysical research communications

authors： Matsumoto Y,Kishida K,Matsumoto M,Matsuoka S,Kohyama M,Suenaga T,Arase H

更新日期：2021-01-01 00:00:00

abstract：BACKGROUND:The activation of hepatic stellate cells plays a central role in the development of liver fibrosis during chronic liver trauma. The aim of the present study was to identify a compound that inhibits the activation process of stellate cells. METHODS:Rat primary cultured stellate cells and a human stellate cel...

journal_title：Biochemical and biophysical research communications

authors： Cui W,Wang M,Maegawa H,Teranishi Y,Kawada N

更新日期：2012-08-31 00:00:00

abstract：:The susceptibility to hydrolysis of LHRH and the decapeptide analogue Antide has been compared. The hydrolysis of LHRH by pig kidney brush border membranes is inhibited by phosphoramidon (I50 = 5.6 nM) implicating endopeptidase-24.11 in the initiation of hydrolysis. Under conditions in which LHRH is fully degraded by ...

journal_title：Biochemical and biophysical research communications

authors： Medeiros Mdos S,Turner AJ,Ljungqvist A,Folkers K

更新日期：1991-04-15 00:00:00

abstract：:VEGF family members play important roles in angiogenesis and vascular permeability. VEGF-A-transgenic mice showed an increased vascularization with edema due to hyper-vascular permeability and subcutaneous hemorrhage as side effects. VEGF-A binds and activates two receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). To...

journal_title：Biochemical and biophysical research communications

authors： Kiba A,Sagara H,Hara T,Shibuya M

更新日期：2003-02-07 00:00:00

abstract：:We demonstrated lanthionine introduction into hexa-histidine-tagged (His-tagged) nukacin ISK-1 prepeptide NukA by modification enzyme NukM in Escherichia coli. Co-expression of nukA and nukM, purification of the resulting His-tagged prepeptide by affinity chromatography, and subsequent mass spectrometry analysis showe...

journal_title：Biochemical and biophysical research communications

authors： Nagao J,Harada Y,Shioya K,Aso Y,Zendo T,Nakayama J,Sonomoto K

更新日期：2005-10-21 00:00:00

abstract：:To determine whether the cytokine interleukin (IL)-6 induces heat shock protein (HSP) 72 gene expression in skeletal muscle, 18 healthy, young men had either a high dose of IL-6 (HiIL-6; n=6), low dose IL-6 (LoIL-6; n=6), or saline (CON; n=6) infused into one femoral artery for 3h. Muscle biopsies were obtained from t...

journal_title：Biochemical and biophysical research communications

authors： Febbraio MA,Steensberg A,Fischer CP,Keller C,Hiscock N,Pedersen BK

更新日期：2002-09-06 00:00:00

abstract：:Human serum albumin (HSA) serves as a natural depot of amyloid β peptide (Aβ). Improvement of Aβ binding to HSA should impede Alzheimer's disease (AD). We developed a method for quantitation of the interaction between monomeric Aβ40/42 and HSA using surface plasmon resonance spectroscopy. The dissociation constant of ...

journal_title：Biochemical and biophysical research communications

authors： Litus EA,Kazakov AS,Sokolov AS,Nemashkalova EL,Galushko EI,Dzhus UF,Marchenkov VV,Galzitskaya OV,Permyakov EA,Permyakov SE

更新日期：2019-03-05 00:00:00

abstract：:Insulin signaling can be negatively regulated by phosphorylation of serine 307 of the insulin receptor substrate (IRS)-1. Rapamycin, an inhibitor of the kinase mTOR, can prevent serine 307 phosphorylation and the development of insulin resistance. We further investigated the role of mTOR in regulating serine 307 phosp...

journal_title：Biochemical and biophysical research communications

authors： Carlson CJ,White MF,Rondinone CM

更新日期：2004-04-02 00:00:00

abstract：:The nucleotide sequence for mouse calcitonin was determined from a cDNA obtained using a polymerase chain reaction (PCR) based method, the rapid amplification of cDNA ends (RACE)-PCR. Primers designed from highly conserved regions in the coding sequences of known rat and human calcitonin cDNAs were used to amplify cal...

journal_title：Biochemical and biophysical research communications

authors： Rehli M,Luger K,Beier W,Falk W

更新日期：1996-09-13 00:00:00

abstract：:miR-4458, a new tumor-suppressor, was reported to down-regulated in human hepatocellular carcinoma. The expression status, roles and inhibitory mechanisms of miR-4458 in other tumors still need to be clarified. The aim of this study is to investigate the effects of miR-4458 and to elucidate the potential mechanism in ...

journal_title：Biochemical and biophysical research communications

authors： Qin Y,Cheng C,Lu H,Wang Y

更新日期：2016-01-01 00:00:00

abstract：:The NC1 domains from the different α(IV) collagen chains were found to exert anti-tumorigenic and/or anti-angiogenic activities. A limitation to the therapeutic use of these matrikines is the large amount of purified recombinant proteins, in the milligram range in mice that should be administered daily throughout the ...

journal_title：Biochemical and biophysical research communications

authors： Thevenard J,Ramont L,Mir LM,Dupont-Deshorgue A,Maquart FX,Monboisse JC,Brassart-Pasco S

更新日期：2013-03-22 00:00:00

abstract：:We have shown previously that aspirin (ASA) ingestion by normal human volunteers inhibits peripheral blood monocyte phospholipase C (PLC) activities ex vivo. In order to explore further the mechanism of action of ASA, normal human monocytes and differentiated human U937 cells were treated with ASA and other salicylate...

journal_title：Biochemical and biophysical research communications

authors： Bomalaski JS,Hirata F,Clark MA

更新日期：1986-08-29 00:00:00

abstract：:Crude membrane fractions prepared from the superficial cortical fiber cells of bovine lenses were incubated with [gamma-32P]-ATP. Membrane-associated 4.1-like proteins were then immunoprecipitated and analyzed for 32P-incorporation. Band 4.1-like proteins of 150 kd, 80 kd, and 78 kd were found to be labeled in a time-...

journal_title：Biochemical and biophysical research communications

authors： Aster JC,Brinn K,Maisel H,Brewer GJ

更新日期：1985-02-15 00:00:00

abstract：:Treatment with double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various invertebrate species. However, it has not yet been reported that this technique can be applied to vertebrates as well. We have investigated whether dsRNA treatment will inhibit gene function in zebrafi...

journal_title：Biochemical and biophysical research communications

authors： Wargelius A,Ellingsen S,Fjose A

更新日期：1999-09-16 00:00:00

abstract：:The antiherpetic agent 9-beta-D-arabinofuranosyladenine (araA) needs to be phosphorylated to its 5'-triphosphate to be effective as an inhibitor of herpes simplex virus replication. Adenosine kinase and deoxycytidine kinase are assumed to convert araA to its 5'-monophosphate. We now found that araAMP is converted to i...

journal_title：Biochemical and biophysical research communications

authors： Balzarini J,De Clercq E

更新日期：1990-12-31 00:00:00

abstract：:Calreticulin isolated from spinach leaves has been specifically phosphorylated in vitro by protein kinase CK2 while animal calreticulin from rabbit liver is not a substrate of this kinase under the same conditions. Phosphoserine is the only phosphoamino acid detected. High affinity binding (Km = 4.4 microM) and a near...

journal_title：Biochemical and biophysical research communications

authors： Baldan B,Navazio L,Friso A,Mariani P,Meggio F

更新日期：1996-04-25 00:00:00

abstract：:Leukaemia inhibitory factor (LIF) was the first soluble factor identified as having potential to maintain the pluripotency of mouse embryonic stem (ES) cells. Recently, a second factor, Wnt, with similar activity was found. However, the relationship between these completely different signals mediating the overlapping ...

journal_title：Biochemical and biophysical research communications

authors： Ogawa K,Nishinakamura R,Iwamatsu Y,Shimosato D,Niwa H

更新日期：2006-04-28 00:00:00

abstract：:Starch branching enzyme (SBE) catalyzes the cleavage of α-1,4-linkages and the subsequent transfer of α-1,4 glucan to form an α-1,6 branch point in amylopectin. We determined the crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose at a resolution of 2.2Å. Maltopentaose bound to a hydro...

journal_title：Biochemical and biophysical research communications

authors： Chaen K,Noguchi J,Omori T,Kakuta Y,Kimura M

更新日期：2012-08-03 00:00:00

abstract：:An infection of any part of female reproductive tract can severely interfere with fertility and reproduction. The fluids and epithelium from the lumen of the female reproductive tract (uterus, oviduct and ovarian follicle) are a known source of antimicrobial action in several species. In this study, we compared the an...

journal_title：Biochemical and biophysical research communications

authors： Dráb T,Kračmerová J,Hanzlíková E,Černá T,Litváková R,Pohlová A,Tichá M,Přikryl P,Liberda J

更新日期：2014-01-17 00:00:00

abstract：:In the rat, pancreatic HCO(-)(3) secretion is believed to be mediated by duct cells with an apical Cl(-)/HCO(-)(3) exchanger acting in parallel with a cAMP-activated Cl(-) channel and protons being extruded through a basolateral Na(+)/H(+) exchanger. However, this may not be the only mechanism for HCO(-)(3) secretion ...

journal_title：Biochemical and biophysical research communications

authors： Thévenod F,Roussa E,Schmitt BM,Romero MF

更新日期：1999-10-14 00:00:00

abstract：:Earlier, we showed that antibodies specific for human serum albumin are able to bind to erythrocyte-associated albumin and inhibit complement mediated hemolysis. In the present study we determine if inhibition is occurring at the membrane attack phase of complement activation or at an earlier step. We show that althou...

journal_title：Biochemical and biophysical research communications

authors： Houle JJ,Hoffmann EM

更新日期：1994-04-15 00:00:00