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Double-stranded RNA induces specific developmental defects in zebrafish embryos.

Abstract：

:Treatment with double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various invertebrate species. However, it has not yet been reported that this technique can be applied to vertebrates as well. We have investigated whether dsRNA treatment will inhibit gene function in zebrafish embryos. By microinjecting dsRNA corresponding to three genetically characterised genes we produced embryonic defects that were similar to the known mutant phenotypes of these loci. The efficiency of inducing specific defects (20-30%) was about 10-fold higher than in experiments with antisense RNA. We also observed that the level of the endogenous mRNA in zebrafish embryos was substantially reduced throughout the embryo following dsRNA injection. However, the interference of gene function showed a strong dependence on the amount of dsRNA. These findings suggest that dsRNA-mediated interference will become an important tool for analysing the functional roles of genes in zebrafish and other vertebrates.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wargelius A,Ellingsen S,Fjose A

doi

10.1006/bbrc.1999.1343

subject

Has Abstract

pub_date

1999-09-16 00:00:00

pages

156-61

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(99)91343-8

journal_volume

263

pub_type

杂志文章

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