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Expression of the human follicle-stimulating hormone receptor in the baculovirus system.

Abstract：

:Human follitropin receptor (hFSHR) cDNA was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter. Spodoptera frugiperda (Sf9) insect cells expressed the hFSHR at the membrane level, 2-3 days after infection. Ligand binding analysis demonstrated saturable, high-affinity binding of 125I-labeled recombinant hFSH (Kd = 0.17 nM) as well as binding specificity for both human and equine FSH. Interestingly, a highly purified preparation of equine choriogonadotropin was found to compete with the binding of 125I-rhFSH. Finally, Western-blot analysis, using a monoclonal antipeptide antibody directed to the 178-206 region of the hFSHR, revealed two bands migrating at M(r) 77 and 130 kDa.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Christophe S,Robert P,Maugain S,Bellet D,Koman A,Bidart JM

doi

10.1006/bbrc.1993.2263

subject

Has Abstract

pub_date

1993-10-15 00:00:00

pages

402-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(83)72263-1

journal_volume

196

pub_type

杂志文章

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