当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Evidence as a HIV-1 self-defense vaccine of cyclic chimeric dodecapeptide warped from undecapeptidyl arch of extracellular loop 2 in both CCR5 and CXCR4.

Evidence as a HIV-1 self-defense vaccine of cyclic chimeric dodecapeptide warped from undecapeptidyl arch of extracellular loop 2 in both CCR5 and CXCR4.

Abstract：

:Novel conformation-specific antibodies were raised against a cyclic chimeric dodecapeptidyl multiple antigen peptide (cCD-MAP) constructed with a spacer-armed Gly-Asp dipeptide and two pentapeptides (S(169)-Q(170)-K(171)-E(172)-G(173) of CCR5 and E(179)-A(180)-D(181)-D(182)-R(183) of CXCR4) which are components of the undecapeptidyl arch (UPA: from R(168) to C(178) in CCR5, from N(176) to C(186) in CXCR4) of extracellular loop 2 (ECL2) in chemokine receptors (CCR5 and CXCR4). Of the antibodies raised, one monoclonal antibody, CPMAb-I (IgMkappa), reacted with cCD-MAP, but not with the linear chimeric dodecapeptide-MAP. The antibody reacted with the cells separately expressing CCR5 or CXCR4, but not with those not expressing the coreceptors. Moreover, the antibody markedly suppressed infection by X4, R5, or R5X4 virus in a dose-dependent manner in a new phenotypic assay for drug susceptibility of HIV-1 using CCR5-expressing Hela/CD4(+) cell clone 1-10 (MAGIC-5). Moreover, CPMAb-I interfered with LAV-1(BRU) infection (m.o.i. = 0.01) of Molt4#8 cells cocultured with CPMAb-I-producing hybridoma in the transwell, and significantly interfered with neither chemotaxis nor calcium influx induced with stromal cell-derived factor 1 alpha (SDF-1alpha). Thus, the antibody raised against the cCD-MAP provides powerful protection or defense against HIV-1 infection. We therefore propose the cCD-MAP or its derivative immunogen as a novel candidate for an HIV-1 coreceptor-based self-defense vaccine.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Misumi S,Takamune N,Ido Y,Hayashi S,Endo M,Mukai R,Tachibana K,Umeda M,Shoji S

doi

10.1006/bbrc.2001.5267

subject

Has Abstract

pub_date

2001-08-03 00:00:00

pages

1309-16

issue

5

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(01)95267-2

journal_volume

285

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Effect of ionomycin on cell pH in isolated renal proximal tubules.

    abstract：:In isolated rabbit proximal tubules the addition of 2.0 microM but not 0.2 microM ionomycin induced a sustained increase in cell pH ([pH]i). This [pH]i response to 2.0 microM ionomycin was shown to be independent of several transporters such as Na+/H+ exchanger, Na(+)-HCO3- cotransporter, Cl-/HCO3- exchanger, or H(+)-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1156

    authors： Yamada H,Seki G,Taniguchi S,Uwatoko S,Suzuki K,Kurokawa K

    更新日期：1996-08-05 00:00:00

  • Complete amino acid sequence of biliverdin-IX beta reductase from human liver.

    abstract：:The amino acid sequence of biliverdin-IX beta reductase (EC1.3.1.24) from human liver was determined by automated Edman degradation of peptides generated by enzymatic and chemical cleavages. The enzyme was a single polypeptide chain of 204 amino acid residues, and its amino acid sequence had no significant homology to...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2649

    authors： Yamaguchi T,Komuro A,Nakano Y,Tomita M,Nakajima H

    更新日期：1993-12-30 00:00:00

  • Isolation and crystallization of functionally competent Escherichia coli peptide deformylase forms containing either iron or nickel in the active site.

    abstract：:Three metallo forms of peptide deformylase (PDF, EC 3.5.1.31) of Escherichia coli were prepared and crystallized (space group C2, diffraction limit 1.9 A) for initiating the X-ray structure determination of the metal center in correlation with the catalytic functionality of this enzyme. The native Fe2+ containing enzy...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8616

    authors： Groche D,Becker A,Schlichting I,Kabsch W,Schultz S,Wagner AF

    更新日期：1998-05-19 00:00:00

  • Involvement of a common 10-amino-acid segment in the cytoplasmic region of CD40 but different MAP kinases in different CD40-mediated responses.

    abstract：:CD40-mediated signals can induce cell aggregation, proliferation and rescue from apoptosis in WEHI231. To define which segment of cytoplasmic domain of CD40 and how signals are involved in those events, we generated mutant CD40 transfectants. We demonstrated the same 10 amino acid segment that could bind to tumor necr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6343

    authors： Hara T,Murakami M,Maeda H,Hibi M,Uede T

    更新日期：1997-04-07 00:00:00

  • CRY2 is suppressed by FOXM1 mediated promoter hypermethylation in breast cancer.

    abstract：:Loss of CRY2 confers aggressive phenotypes to breast cancer. However, the mechanism of its downregulation and its prognostic value in breast cancer are still not clear. Our data mining in TCGA breast cancer cohort (TCGA-BRCA) showed that the luminal A subtype of breast cancer had the highest CRY2 expression, while the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.003

    authors： Liu L,Shen H,Wang Y

    更新日期：2017-08-12 00:00:00

  • Absence of inactivation or phosphorylation of ornithine decarboxylase by nuclear protein kinase NII and of immunological cross-reactivity between RNA polymerase I and ornithine decarboxylase.

    abstract：:Incubation with protein kinase NII did not result in phosphorylation or inactivation of mouse kidney ornithine decarboxylase. Partially purified ornithine decarboxylase preparations contained a protein kinase activity and stimulated the activity of RNA polymerase I. However, these properties were due to contaminating ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91436-0

    authors： Seely JE,Stetler DA,Jacob ST,Pegg AE

    更新日期：1984-04-16 00:00:00

  • Phosphorylation pattern of a 25 Kdalton stress protein from rat myoblasts.

    abstract：:Phosphorylation of a 25 Kdalton nuclear stress protein from rat muscle was examined by two-dimensional gel electrophoresis and one-dimensional peptide mapping. These studies show that three 25 Kdalton stress proteins found in stressed rat myoblasts are actually the same protein with charge variation brought about by m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91651-0

    authors： Kim YJ,Shuman J,Sette M,Przybyla A

    更新日期：1983-12-28 00:00:00

  • Regulation of p56(lck) messenger turnover upon T cell activation: involvement of the 3' untranslated region in stability as determined in cell-free extracts.

    abstract：:Full activation of T lymphocytes transiently downregulates the steady state level of the tyrosine kinase p56(lck) mRNA. Here, we show that a decrease in messenger stability is involved in this downmodulation followed thereafter by a rapid and marked increase in mRNA half-life. In order to facilitate the study of p56(l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6477

    authors： Vanhée-Brossollet C,Vaquero C

    更新日期：1997-04-17 00:00:00

  • A single nucleotide polymorphism of CYP2b6 found in Japanese enhances catalytic activity by autoactivation.

    abstract：:A single nucleotide polymorphism (SNP) resulting in a substitution from Gln to His was found in exon 4 of the CYP2B6 gene in Japanese. The frequency of the variant allele was found to be 19.9%. The mutant- and the wild-type enzymes were expressed in Escherichia coli, and the effects of the single amino acid substituti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4524

    authors： Ariyoshi N,Miyazaki M,Toide K,Sawamura Yi,Kamataki T

    更新日期：2001-03-01 00:00:00

  • Effect of ATP binding cassette/multidrug resistance proteins on ATP efflux of Saccharomyces cerevisiae.

    abstract：:Multidrug resistance (MDR) in mammalian tumors or tissues is often associated with the overexpression of the putative drug efflux pump P-glycoprotein (Pgp). One theory concerning the mechanism of Pgp activity is that efflux of ATP is coupled to drug efflux. Evidence in support of this theory has been observed in mamma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.5913

    authors： Boyum R,Guidotti G

    更新日期：1997-01-03 00:00:00

  • Gamma-glutamyltranspeptidase-dependent glutathione catabolism results in activation of NF-kB.

    abstract：:gamma-glutamyltranspeptidase (GGT) is a key enzyme implicated in the homeostasis of intracellular reduced glutathione (GSH) and hence in the regulation of the cellular redox state. Besides, the extracellular cleavage of GSH by GGT leads to reactive oxygen species (ROS) production, depending on the generation and enhan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3585

    authors： Accaoui MJ,Enoiu M,Mergny M,Masson C,Dominici S,Wellman M,Visvikis A

    更新日期：2000-10-05 00:00:00

  • Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells.

    abstract：:The acidic leucine-rich nuclear phosphoprotein 32B (ANP32B) is a member of a conserved superfamily of nuclear proteins whose functions are largely unknown. In our previous work, ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. In this work, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.06.025

    authors： Yu Y,Shen SM,Zhang FF,Wu ZX,Han B,Wang LS

    更新日期：2012-07-13 00:00:00

  • Expression of 5-oxoETE receptor in prostate cancer cells: critical role in survival.

    abstract：:Previously, we reported that metabolism of arachidonic acid through the 5-lipoxygenase (5-LOX) pathway plays an important role in the survival and growth of human prostate cancer cells. Inhibition of 5-LOX by pharmacological inhibitors triggers apoptosis in prostate cancer cells within hours of treatment, which is pre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.189

    authors： Sundaram S,Ghosh J

    更新日期：2006-01-06 00:00:00

  • Characterization of TRIM62 as a RING finger E3 ubiquitin ligase and its subcellular localization.

    abstract：:TRIM62, also named DEAR1, is a member of the TRIM/RBCC family, which includes proteins with conserved RING finger, B-box and coiled-coil domains. Several reports have identified a role for this family in cancer, retroviral infection and innate immunity. In this study, the E3 ubiquitin ligase activity and subcellular l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.02.012

    authors： Huang F,Xiao H,Sun BL,Yang RG

    更新日期：2013-03-08 00:00:00

  • Identification of stress-tolerance-related transcription-factor genes via mini-scale Full-length cDNA Over-eXpressor (FOX) gene hunting system.

    abstract：:Recently, we developed a novel system known as Full-length cDNA Over-eXpressor (FOX) gene hunting [T. Ichikawa, M. Nakazawa, M. Kawashima, H. Iizumi, H. Kuroda, Y. Kondou, Y. Tsuhara, K. Suzuki, A. Ishikawa, M. Seki, M. Fujita, R. Motohashi, N. Nagata, T. Takagi, K. Shinozaki, M. Matsui, The FOX hunting system: an alt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.09.124

    authors： Fujita M,Mizukado S,Fujita Y,Ichikawa T,Nakazawa M,Seki M,Matsui M,Yamaguchi-Shinozaki K,Shinozaki K

    更新日期：2007-12-14 00:00:00

  • Cardioprotective effects of recombinant human erythropoietin in rats with experimental autoimmune myocarditis.

    abstract：:Erythropoietin (EPO) has been known to have cytoprotective effects on several types of tissues, presumably through modulation of apoptosis and inflammation. The effect of EPO on myocardial inflammation, however, has not yet been clarified. We investigated the cardioprotective effects of EPO in rats with experimental a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.03.230

    authors： Mitsuma W,Ito M,Kodama M,Fuse K,Okamura K,Minagawa S,Kato K,Hanawa H,Toba K,Nakazawa M,Aizawa Y

    更新日期：2006-06-09 00:00:00

  • Marked rapid alterations in nocturnal pineal serotonin metabolism in mice and rats exposed to weak intermittent magnetic fields.

    abstract：:Adult AMES mice and male Sprague Dawley rats were exposed to an artificial magnetic field, generated by Helmholtz coils. 3.5 hours after the onset of darkness the coils were activated for one hour resulting in an inversion of the horizontal component of the earth's magnetic field. The coils were activated and deactiva...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91439-y

    authors： Lerchl A,Nonaka KO,Stokkan KA,Reiter RJ

    更新日期：1990-05-31 00:00:00

  • In vitro synthesis of a plant phospholipid transfer protein: a study by high performance liquid chromatography.

    abstract：:In order to study the biosynthesis of a plant phospholipid transfer protein (PLTP), poly (A)+RNAs have been prepared from maize seedlings and translated in vitro with a rabbit reticulocyte lysate. The newly synthesized proteins were then separated by fast protein liquid chromatography (FPLC) followed by SDS-PAGE or by...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91843-1

    authors： Tchang F,Laroche-Raynal M,Vergnolle C,Demandre C,Douady D,Grosbois M,Guerbette F,Delseny M,Kader JC

    更新日期：1985-11-27 00:00:00

  • Molecular basis of bird respiration: primary hemoglobin structure component from Tufted duck (Aythya fuligula, Anseriformes)--role of alpha99Arg in formation of a complex salt bridge network.

    abstract：:The primary structure of the major hemoglobin component, HbA (alpha(A)- and beta-chain), from Tufted duck (Aythya fuligula) is presented. The separation of the globin subunits was achieved by ion exchange chromatography on CM-cellulose in 8 M urea. The amino acid sequence was determined by automatic Edman degradation ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6399

    authors： Abbasi A,Lutfullah G

    更新日期：2002-02-15 00:00:00

  • Small molecule inhibition of arylamine N-acetyltransferase Type I inhibits proliferation and invasiveness of MDA-MB-231 breast cancer cells.

    abstract：:Arylamine N-acetyltransferase 1 is a phase II metabolizing enzyme that has been associated with certain breast cancer subtypes. While it has been linked to breast cancer risk because of its role in the metabolic activation and detoxification of carcinogens, recent studies have suggested it may be important in cell gro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.087

    authors： Tiang JM,Butcher NJ,Minchin RF

    更新日期：2010-02-26 00:00:00

  • Acute stress enhances learning and memory by activating acid-sensing ion channels in rats.

    abstract：:Acute stress has been shown to enhance learning and memory ability, predominantly through the action of corticosteroid stress hormones. However, the valuable targets for promoting learning and memory induced by acute stress and the underlying molecular mechanisms remain unclear. Acid-sensing ion channels (ASICs) play ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.122

    authors： Ye S,Yang R,Xiong Q,Yang Y,Zhou L,Gong Y,Li C,Ding Z,Ye G,Xiong Z

    更新日期：2018-04-15 00:00:00

  • Lycopene suppresses ovalbumin-induced airway inflammation in a murine model of asthma.

    abstract：:In this study, we attempt to determine whether lycopene regulates inflammatory mediators in the ovalbumin (OVA)-induced murine asthma model. To address this, mice were sensitized and challenged with OVA, and then treated with lycopene before the last OVA challenge. Administration of lycopene significantly alleviated t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.07.032

    authors： Lee CM,Chang JH,Moon DO,Choi YH,Choi IW,Park YM,Kim GY

    更新日期：2008-09-19 00:00:00

  • A large-molecular-weight regulating factor for chromatin-dependent RNA polymerase II reactions in rat liver cytoplasm.

    abstract：:The author found a sugar-containing 70 KDa factor in rat liver nuclei as described in the previous report (1), which repressed RNA chain initiation by all classes of rat liver RNA polymerases and specifically stimulated chromatin-dependent RNA chain elongation catalyzed by RNA polymerase II. In this communication, the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80366-6

    authors： Okai Y

    更新日期：1984-11-30 00:00:00

  • Inhibition of the catalytic subunit of ram sperm adenylate cyclase by adenosine.

    abstract：:Adenosine inhibits ram sperm adenylate cyclase activity which is membrane-bound and comprises only the catalytic subunit. The inhibition parameters of adenylate cyclase by adenosine were not modified when the enzyme was purified 3 to 5,000 fold. Optimal inhibition by adenosine was found to require a high concentration...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90320-7

    authors： Henry D,Ferino F,Tomova S,Ferry N,Stengel D,Hanoune J

    更新日期：1986-06-30 00:00:00

  • RBM14 is indispensable for pluripotency maintenance and mesoderm development of mouse embryonic stem cells.

    abstract：:The pluripotency of embryonic stem cells (ESCs) is maintained by core pluripotency transcription factors, cofactors and several signaling pathways. RBM14 is a component of the para-speckle complex, which has been implicated in multiple important biological processes. The role of RBM14 in ESCs and lineage differentiati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.231

    authors： Chen G,Zhang D,Zhang L,Feng G,Zhang B,Wu Y,Li W,Zhang Y,Hu B

    更新日期：2018-06-18 00:00:00

  • Transcriptional inhibitors affecting topoisomerase II induce changes in histone methylation patterns similar to those induced by heat shock.

    abstract：:The relationship between gene expression and the patterns of histone methylation in Drosophila cells has been investigated using inhibitors of transcription acting at various levels. Inhibition of ribosomal RNA synthesis and processing by 5-fluorouridine or of general RNA synthesis by camptothecin, an inhibitor of top...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90778-x

    authors： Desrosiers R,Tanguay RM

    更新日期：1989-08-15 00:00:00

  • Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon.

    abstract：:Cultivated rice (Oryza sativa) possesses various labdane-related diterpene synthase genes, homologs of ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) that are responsible for the biosynthesis of phytohormone gibberellins. The CPS homologs and KS like (KSL) homologs successively converted geranylg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.10.062

    authors： Toyomasu T,Miyamoto K,Shenton MR,Sakai A,Sugawara C,Horie K,Kawaide H,Hasegawa M,Chuba M,Mitsuhashi W,Yamane H,Kurata N,Okada K

    更新日期：2016-11-18 00:00:00

  • Activation of calcium-sensing receptor-mediated autophagy in angiotensinII-induced cardiac fibrosis in vitro.

    abstract：:Cardiac fibrosis is one of the primary mechanisms of ventricular remodeling, and there is no effective method for reversal. Activation of calcium sensing receptor (CaSR) has been reported to be involved in the development of myocardial fibrosis, but the molecular mechanism for CaSR activation has not yet been clarifie...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.098

    authors： Chi J,Wang L,Zhang X,Fu Y,Liu Y,Chen W,Liu W,Shi Z,Yin X

    更新日期：2018-03-04 00:00:00

  • Phosphorylation of VASP by AMPK alters actin binding and occurs at a novel site.

    abstract：:Vasodilator-stimulated phosphoprotein (VASP) is an actin regulatory protein that functions in adhesion and migration. In epithelial cells, VASP participates in cell-cell adhesion. At the molecular level, VASP drives actin bundling and polymerization. VASP activity is primarily regulated by phosphorylation. Three physi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.09.059

    authors： Thomson DM,Ascione MP,Grange J,Nelson C,Hansen MD

    更新日期：2011-10-14 00:00:00

  • Involvement of TOR signaling motif in the regulation of plant autophagy.

    abstract：:In our previous studies, we have demonstrated that a stretch of amino-acid sequences identified from Arabidopsis ribosomal S6 kinase 1 (AtS6K1) provided a plant version of the TOS (TOR-signaling) motif, mediating the interaction with the Raptor protein in the TOR (Target of Rapamycin) kinase complex. Here we report th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.027

    authors： Son O,Kim S,Kim D,Hur YS,Kim J,Cheon CI

    更新日期：2018-06-27 00:00:00