Abstract:
:In order to study the biosynthesis of a plant phospholipid transfer protein (PLTP), poly (A)+RNAs have been prepared from maize seedlings and translated in vitro with a rabbit reticulocyte lysate. The newly synthesized proteins were then separated by fast protein liquid chromatography (FPLC) followed by SDS-PAGE or by high performance liquid chromatography (HPLC) coupled to a radioactivity detector monitor. It has been showed that a radioactive band comigrating with a 14C methylated pure PLTP was detected by SDS-PAGE. This result, confirmed by direct radioactive monitoring, indicates that PLTP is actively synthesized in vitro.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Tchang F,Laroche-Raynal M,Vergnolle C,Demandre C,Douady D,Grosbois M,Guerbette F,Delseny M,Kader JCdoi
10.1016/0006-291x(85)91843-1subject
Has Abstractpub_date
1985-11-27 00:00:00pages
75-81issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(85)91843-1journal_volume
133pub_type
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