Effect of botulinum D toxin on neutrophils.

abstract：:The Bombyx mori nucleopolyhedrovirus (BmNPV) ie-1 promoter exhibits strong transcriptional activity and is used in transient foreign gene expression systems in insect cells. In a reporter assay experiment using the BmNPV ie-1 promoter, we found that it exhibited activity even in non-host mammalian BHK cells, plant BY-...

journal_title：Biochemical and biophysical research communications

authors： Fujita R,Ono C,Ono I,Asano SI,Bando H

更新日期：2015-09-04 00:00:00

abstract：:Two adenylate cyclase inhibitors: 9-(tetrahydro-2-furyl)adenine and 2'5'-dideoxyadenosine decreased cAMP levels in LH-stimulated immature rat Leydig cells by 20-40%, independent of the concentration of LH. Steroid production was not correlated with this decrease in cAMP, but was increased (146%). The phorbol ester 4 b...

journal_title：Biochemical and biophysical research communications

authors： Themmen AP,Hoogerbrugge JW,Rommerts FF,van der Molen HJ

更新日期：1985-05-16 00:00:00

abstract：:Human immunodeficiency virus type 1 Vpr is a 96-amino-acid virion-associated protein that arrests cells in the G2 phase of the cell cycle in peripheral blood lymphocytes, HeLa, 293, 293T, A549, Jurkat, CEM, SupT1, CV-1 and COS1 cells. When we transfected Vpr expression vector into mouse NIH3T3 and then cultured it in ...

journal_title：Biochemical and biophysical research communications

authors： Nishino Y,Myojin T,Kamata M,Aida Y

更新日期：1997-03-17 00:00:00

abstract：:Transgenic mice were produced by the microinjection of the cDNA of human alpha-fetoprotein (AFP) under control of the enhancer/promoter of the human beta-actin gene. Among the 4 mouse lines where the transgene were stable transmitted to the progeny, 3 produced human AFP. The expression was not developmental stage-spec...

journal_title：Biochemical and biophysical research communications

authors： Yamashita T,Kasai N,Miyoshi I,Sasaki N,Maki K,Sakai M,Nishi S,Namioka S

更新日期：1993-03-15 00:00:00

abstract：:It has long been assumed that Ca2+ are translocated from the cytosol to the cell nucleus by a long distance to activate transcription machinery buried deep in the nucleoplasm. However, this model has been recently challenged. When HeLa cells were loaded with fluo-3, highly fluorescent spots of approximately 2 microns ...

journal_title：Biochemical and biophysical research communications

authors： Lui PP,Kong SK,Kwok TT,Lee CY

更新日期：1998-06-09 00:00:00

abstract：:It is demonstrated that carbon fixation in photosynthesis is regulated in two kinetically coupled pathways involving the specialized pair of non-equivalent, enzyme-bound glycerate-3-P (3-PGA) molecules obtained from ribulose 1,5-bisphosphate (RuBP) carboxylation in the light. A non-cyclic pathway is suggested (reactio...

journal_title：Biochemical and biophysical research communications

authors： Fong FK,Butcher KA

更新日期：1988-01-15 00:00:00

abstract：:The expansion of a CAG trinucleotide repeat (TNR) sequence has been linked to several neurological disorders, for example, Huntington's disease (HD). In HD, healthy individuals have 5-35 CAG repeats. Those with 36-39 repeats have the premutation allele, which is known to be prone to expansion. In the disease state, gr...

journal_title：Biochemical and biophysical research communications

authors： Jarem DA,Delaney S

更新日期：2011-12-09 00:00:00

abstract：:Recombinant human glia maturation factor beta (r-hGMF-beta) is a single-chain polypeptide (141 amino acid residues) containing three cysteines, at positions 7, 86 and 95. Nascent r-hGMF-beta exists in the reduced state and has no biological activity. The protein can be activated through oxidative refolding by incubati...

journal_title：Biochemical and biophysical research communications

authors： Zaheer A,Lim R

更新日期：1990-09-14 00:00:00

abstract：:Various gene delivery systems have been widely studied for the acute spinal cord injury (SCI) treatment. In the present study, a novel type of brain-derived neurotrophic factor (BDNF)-loaded cationic nanobubbles (CNBs) conjugated with MAP-2 antibody (mAbMAP-2/BDNF/CNBs) was prepared to provide low-intensity focused ul...

journal_title：Biochemical and biophysical research communications

authors： Song Z,Ye Y,Zhang Z,Shen J,Hu Z,Wang Z,Zheng J

更新日期：2018-02-12 00:00:00

abstract：:Interleukin-1 alpha induced an increase in both the cellular accumulation of cis-diamminedichloroplatinum (II) (cisplatin) and DNA platination and significantly reduced the removal of platinum from DNA of human ovarian (NIH: OVCAR-3) carcinoma cells in culture. The combinations of IL-1 alpha and cisplatin were highly ...

journal_title：Biochemical and biophysical research communications

authors： Benchekroun MN,Parker R,Reed E,Sinha BK

更新日期：1993-08-31 00:00:00

abstract：:In the previous study (Sato K.-I. et al. (1997) FEBS Lett. 410, 136-140), we showed that the phosphorylation of Shc protein by c-Src is dependent on the binding of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to the PTB domain of Shc. In this study, we demonstrate that, in contrast to c-Src, v-Src and epiderm...

journal_title：Biochemical and biophysical research communications

authors： Sato K,Gotoh N,Otsuki T,Kakumoto M,Aoto M,Tokmakov AA,Shibuya M,Fukami Y

更新日期：1997-11-17 00:00:00

abstract：:Rat mast cell protease of type 1 (RMCP1) is a specific marker of connective tissue mast cells selectively occurring in some tissues, e.g., the tongue. Its amino acid sequence is known (Le Trong et al., Biochem. 1987, 26, 6988-6994) but not the corresponding nucleotide sequence. Amplification of mRNAs from rat tongue w...

journal_title：Biochemical and biophysical research communications

authors： Rouleau A,Garbarg M,Schwartz JC,Ruat M

更新日期：1994-03-15 00:00:00

abstract：:Previous studies have shown that functionalized gold nanoparticles (AuNPs) can be used as a general platform for loading and delivering DNA oligonucleotides and short hairpin RNA to living systems. Here, we report the ability of functionalized AuNP to deliver RNA aptamers into the nuclei of human cells. An in vitro-sy...

journal_title：Biochemical and biophysical research communications

authors： Ryou SM,Kim JM,Yeom JH,Hyun S,Kim S,Han MS,Kim SW,Bae J,Rhee S,Lee K

更新日期：2011-12-09 00:00:00

abstract：:Bovine thyroid 100,000 X g supernatant contained calcium-activated, calmodulin-dependent protein kinase (PK-CaM) activity. The PK-CaM was partially purified using ion-exchange chromatography and characterized. PK-CaM, using casein as exogenous substrate, was not stimulated by Ca2+(0-500 microM) or calmodulin (1-10 mic...

journal_title：Biochemical and biophysical research communications

authors： Friedman Y,Henricks L,Poleck T,Levasseur S,Burke G

更新日期：1986-10-15 00:00:00

abstract：:Angiotensin II (Ang-II) has been shown to possess several atherogenic properties including its ability to induce macrophage-mediated oxidation of LDL and to form Ang-II-modified LDL which is taken up by macrophages at enhanced rate. Oxidized-LDL (Ox-LDL) is also taken up by macrophages at enhanced rate via several sca...

journal_title：Biochemical and biophysical research communications

authors： Keidar S,Attias J

更新日期：1997-10-09 00:00:00

abstract：:We have characterized a novel surface protein from urea extract of whole cells of group A Streptococcus pyogenes (GAS). A major protein band (35kD) was found to hybridize with human IgG by Western blotting. A search of the N-terminal amino acid sequence of this protein by using the GAS genome sequence database reveale...

journal_title：Biochemical and biophysical research communications

authors： Kawabata S,Tamura Y,Murakami J,Terao Y,Nakagawa I,Hamada S

更新日期：2002-09-06 00:00:00

abstract：:Despite decades of intensive studies, the failure to identify plasmodesmata (PD) localization sequences has constrained our understanding of Tobacco mosaic virus (TMV) movement. Recently, we identified the first PD localization signal (major PLS) in the TMV movement protein (MP), which encompasses the first 50 amino a...

journal_title：Biochemical and biophysical research communications

authors： Liu Y,Huang C,Zeng J,Yu H,Li Y,Yuan C

更新日期：2020-01-01 00:00:00

abstract：:Dishevelled (Dvl) is the essential signal transduction component of both canonical and non-canonical Wnt signaling pathways. The cysteine-rich protein Idax acts as a negative regulator of Wnt signaling in mammals by interaction with Dvl in the region of the PDZ domain. In an effort to clarify the structural basis of t...

journal_title：Biochemical and biophysical research communications

authors： London TB,Lee HJ,Shao Y,Zheng J

更新日期：2004-09-10 00:00:00

abstract：:Hypoxia-inducible factors are crucial in the regulatory process of oxygen homeostasis of vertebrate cells. Inhibition of prolyl hydroxylation of HIF-alpha subunits by prolyl-hydroxylases (PHD1, PHD2 and PHD3) leads to transcription of a greater number of hypoxia responsive genes. We have investigated the subcellular d...

journal_title：Biochemical and biophysical research communications

authors： Steinhoff A,Pientka FK,Möckel S,Kettelhake A,Hartmann E,Köhler M,Depping R

更新日期：2009-10-02 00:00:00

abstract：:We investigated the influence of pregnancy on the expression of insulin-regulated glucose transporter (GLUT4) mRNA and protein in skeletal muscle and adipose tissue. GLUT4 mRNA expression in quadriceps muscle from control and pregnant rats was similar in the fasted and fed states. When the level of expression was dete...

journal_title：Biochemical and biophysical research communications

authors： Okuno S,Maeda Y,Yamaguchi Y,Takao Y,Trocino RA,Takino H,Kawasaki E,Yokota A,Uotani S,Akazawa S

更新日期：1993-03-15 00:00:00

abstract：:Viral vectors displaying specific ligand binding moieties have raised an increasing interest in the area of targeted gene therapy. In this report, we describe baculovirus vectors displaying either a functional single chain antibody fragment (scFv) specific for the carcinoembryonic antigen (CEA) or the synthetic IgG bi...

journal_title：Biochemical and biophysical research communications

authors： Ojala K,Mottershead DG,Suokko A,Oker-Blom C

更新日期：2001-06-15 00:00:00

abstract：:Alcohol dehydrogenase (ADH; EC 1.1.1.1) and aldehyde dehydrogenase (ALDH; EC 1.2.1.3.) are important enzymes involved in the biotransformation of both alcohols and aldehydes. Today, six classes of ADH and twelve classes of ALDH have been defined in mammals. Here we report the detection and localisation of three classe...

journal_title：Biochemical and biophysical research communications

authors： Cheung C,Smith CK,Hoog JO,Hotchkiss SA

更新日期：1999-07-22 00:00:00

abstract：:Different groups including ours have shown that curcumin induces melanoma cell apoptosis, here we focused the role of mammalian Sterile 20-like kinase 1 (MST1) in it. We observed that curcumin activated MST1-dependent apoptosis in cultured melanoma cells. MST1 silencing by RNA interference (RNAi) suppressed curcumin-i...

journal_title：Biochemical and biophysical research communications

authors： Yu T,Ji J,Guo YL

更新日期：2013-11-08 00:00:00

abstract：:Currently, a broad diversity of fluorescent proteins among marine organisms range from cyano-red emissions. Fluorescent proteins differ in their DNA sequences from green fluorescent protein (GFP). We identified cDNA encoding the gene of a new protein from the reef coral Hydnophora rigida of the Merulinidae family. Bot...

journal_title：Biochemical and biophysical research communications

authors： Bokhari H,Smith C,Veerendra K,Sivaraman J,Sikaroodi M,Gillevet P

更新日期：2010-06-04 00:00:00

abstract：:Pulmonary fibrosis (PF) is a fatal respiratory disease with no effective medical treatments available. TGF-β/Smads signaling has been implicated to play an essential in the pathogenesis of PF, in which Smad3 act as the integrator of pro-fibrosis signals. In this study, we determined the effect of SIS3, a specific inhi...

journal_title：Biochemical and biophysical research communications

authors： Shou J,Cao J,Zhang S,Sun R,Zhao M,Chen K,Su SB,Yang J,Yang T

更新日期：2018-09-05 00:00:00

abstract：:Heme oxygenase-1 (HO-1) is an inducible form of heme oxygenase that catabolizes heme to carbon monoxide, biliverdin, and ferrous iron. We have investigated whether HO-1 can induce angiogenic effects in vivo. Rats were subjected to a bolus injection of either wild type adenovirus (ad-wt) or adenovirus encoding HO-1 (ad...

journal_title：Biochemical and biophysical research communications

authors： Suzuki M,Iso-o N,Takeshita S,Tsukamoto K,Mori I,Sato T,Ohno M,Nagai R,Ishizaka N

更新日期：2003-02-28 00:00:00

abstract：:The Rh blood group antigens are carried by two distinct but homologous membrane proteins encoded by two closely related genes, RCHE and RHD. Rh50 glyco-protein is the membrane protein tightly associated with Rh polypeptides and is critical for expression of Rh antigens. The amino acid sequence and predicted membrane t...

journal_title：Biochemical and biophysical research communications

authors： Iwamoto S,Omi T,Yamasaki M,Okuda H,Kawano M,Kajii E

更新日期：1998-02-04 00:00:00

abstract：:The influence of isoproterenol (10(-6) M) on junctional conductance of cell pairs isolated from adult rat ventricle was investigated using two separated voltage-clamp circuits. The drug increased the junctional conductance by 40% (S.E. +/- 11%) (n = 10) within 20 sec. In experiments in which an inhibitor of cAMP depen...

journal_title：Biochemical and biophysical research communications

authors： De Mello WC

更新日期：1988-07-29 00:00:00

abstract：:It has been generally viewed that salicylates ameliorate type 2 diabetes through interfering with the NF-kappaB signaling. Earlier studies indicated that IKKbeta was the key for the development of insulin resistance. However, it was unknown whether IKKbeta itself, or its downstream target, NF-kappaB, plays major roles...

journal_title：Biochemical and biophysical research communications

authors： Chen F

更新日期：2005-06-24 00:00:00

abstract：:Lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7] catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol in the reaction that forms the sterol nucleus. We report herein the cloning and characterization of the human gene (OSC) encoding lanosterol synthase, a predi...

journal_title：Biochemical and biophysical research communications

authors： Baker CH,Matsuda SP,Liu DR,Corey EJ

更新日期：1995-08-04 00:00:00