Analysis of the Bombyx mori nucleopolyhedrovirus ie-1 promoter in insect, mammalian, plant, and bacterial cells.

abstract：:Fractionation of rat uterine cells incubated at 22 degrees C with 0.2 nM [3H]-estradiol-17 beta (E2 beta) was performed to analyze the subcellular distribution of internalized hormone. The postnuclear supernatant of homogenates was resolved in Percoll density gradients into six major fractions defined by enzyme marker...

journal_title：Biochemical and biophysical research communications

authors： Pietras RJ,Szego CM

更新日期：1984-08-30 00:00:00

abstract：:Osteoblasts/stromal cells support the formation of osteoclast-like cells (OCL) from osteoclast progenitor cells via expressing a membrane-associated protein, osteoclast differentiation factor (ODF), in the presence of osteotropic factors, whereas the cells secrete a substantial amount of osteoclastogenesis inhibitory ...

journal_title：Biochemical and biophysical research communications

authors： Nagai M,Sato N

更新日期：1999-04-21 00:00:00

abstract：:The Bcl-2 oncoprotein is a potent inhibitor of apoptosis and is overexpressed in a variety of different malignancies. Bcl-2 function is regulated through heterodimerization with other members of the Bcl-2 protein family. In addition, several proteins that are not members of the Bcl-2 family can bind to Bcl-2, includin...

journal_title：Biochemical and biophysical research communications

authors： Antoku K,Maser RS,Scully WJ Jr,Delach SM,Johnson DE

更新日期：2001-09-07 00:00:00

abstract：:To investigate the functions of key domains of the epidermal growth factor receptor (EGFR), various EGFR-derived peptide sequences were expressed in Escherichia coli as glutathione S-transferase (GST) fusion proteins. The purified fusion proteins (GST-TK0-8) were tested as substrates for the tyrosine kinase activities...

journal_title：Biochemical and biophysical research communications

authors： Koland JG,O'Brien KM,Cerione RA

更新日期：1990-01-15 00:00:00

abstract：:The hydrolysis of p-nitrophenylphosphorylcholine, p-nitrophenylphosphate, and phosphorylcholine was quantitated in homogenates prepared from the occipital and temporal regions of control and Alzheimer's Disease patient brain. There were no significant differences detected in activities of enzymes cleaving the first 2 ...

journal_title：Biochemical and biophysical research communications

authors： Kanfer JN,McCartney DG

更新日期：1986-08-29 00:00:00

abstract：:The sulfonation of estrogens by human estrogen sulfotransferase (humSULT1E1) plays a vital role in controlling the active levels of these hormones in the body. To understand more fully the structural and functional characteristics of humSULT1E1, we have carried out site-directed mutagenesis of critical amino acids fou...

journal_title：Biochemical and biophysical research communications

authors： Hempel N,Barnett AC,Bolton-Grob RM,Liyou NE,McManus ME

更新日期：2000-09-16 00:00:00

abstract：:Streptomyces toyocaensis NRRL 15009 produces A47934, a glycopeptide antibiotic. This compound is composed of several unusual amino acids, some of which have chlorinated aromatic rings. We have isolated two distinct halogenating enzymes, a chloroperoxidase (42,882 Da) and a catalase/bromoperoxidase (53,890 Da), from la...

journal_title：Biochemical and biophysical research communications

authors： Marshall GC,Wright GD

更新日期：1996-02-15 00:00:00

abstract：:hPepT1 is a proton-coupled peptide transporter that mediates the absorption of di- and tripeptides. Here we show that tyrosine 167 (Y167) in transmembrane domain 5 (TMD5) of this 12-transmembrane spanning protein contributes to its transport function. We identified this particular amino acid by a computer model of the...

journal_title：Biochemical and biophysical research communications

authors： Yeung AK,Basu SK,Wu SK,Chu C,Okamoto CT,Hamm-Alvarez SF,von Grafenstein H,Shen WC,Kim KJ,Bolger MB,Haworth IS,Ann DK,Lee VH

更新日期：1998-09-08 00:00:00

abstract：:Polyphosphoinositide hydrolysis was examined in membranes from thyrotropin-releasing hormone (TRH)-responsive GH3 pituitary cells. [3H]Inositol phosphates (IP2 and IP3) were generated upon incubation of membranes from [3H]inositol-labeled cells indicating the presence of a membrane-associated polyphosphoinositide phos...

journal_title：Biochemical and biophysical research communications

authors： Lucas DO,Bajjalieh SM,Kowalchyk JA,Martin TF

更新日期：1985-10-30 00:00:00

abstract：:Eicosapentaenoic acid (EPA), an n-3 polyunsaturated fatty acid (PUFA), has been shown to decrease the risk of atherosclerosis by attenuating endothelial activation. In this study, we used mass spectrometry-based label-free quantitative proteomics to study the protective mechanisms of EPA and to identify key proteins t...

journal_title：Biochemical and biophysical research communications

authors： Zhang L,Xiao K,Zhao X,Sun X,Zhang J,Wang X,Zhu Y,Zhang X

更新日期：2017-05-27 00:00:00

abstract：:Skinned muscle fibers prepared from fetal rabbit heart (28 days of gestation) showed a marked resistance to acidic pH in the Ca(2+) regulation of force generation, compared to the fibers prepared from adult heart. SDS-PAGE and immunoblot analysis showed that the slow skeletal troponin I was predominantly expressed in ...

journal_title：Biochemical and biophysical research communications

authors： Morimoto S,Goto T

更新日期：2000-01-27 00:00:00

abstract：:The EC(50) values for concentration-dependent stimulation of cAMP accumulation by CRF (1.3nM) and urocortin (1.0nM) were equivalent in human retinoblastoma Y79 cells. The time course and magnitude of CRF- and urocortin-induced CRF(1) receptor desensitization were similar. A significant 3-fold increase in GRK3, but not...

journal_title：Biochemical and biophysical research communications

authors： Dautzenberg FM,Wille S,Braun S,Hauger RL

更新日期：2002-11-01 00:00:00

abstract：:Overexpression of the ubiquitin-conjugating enzyme Cdc34 conferred strong cadmium resistance on budding yeast. Proteasome activity, which is involved in the degradation of ubiquitinated proteins, was not essential for the acquisition of resistance to cadmium. The overexpression of Cdc34 accelerated the ubiquitination ...

journal_title：Biochemical and biophysical research communications

authors： Hwang GW,Furuchi T,Naganuma A

更新日期：2007-11-23 00:00:00

abstract：:Perforin, a potent pore-forming protein, plays an important role in killer lymphocyte-mediated cytolysis. The studies on perforin, although already extensive, have been hampered by the limited amount of perforin naturally available from killer lymphocytes. In the present study, a full-length mouse perforin was express...

journal_title：Biochemical and biophysical research communications

authors： Liu CC,Persechini PM,Young JD

更新日期：1994-05-30 00:00:00

abstract：:Baicalin (BA) has been shown with anti-HIV-1 activity. Zinc is a nutrient element. The anti-HIV-1 activity of zinc complex of baicalin (BA-Zn) in vitro was studied and compared with the anti-HIV-1 activities between BA and BA-Zn in the present study. Our results suggested that BA-Zn has lower cytotoxicity and higher a...

journal_title：Biochemical and biophysical research communications

authors： Wang Q,Wang YT,Pu SP,Zheng YT

更新日期：2004-11-12 00:00:00

abstract：:Britannin (Bri), isolated from Inula aucheriana, is a sesquiterpene lactone (SL), a class of secondary metabolites. Previous studies have suggested the anti-cancer potential of Bri; however, the molecular mechanism remains elusive. The present study investigated the effects of Bri on liver cancer progression. Our find...

journal_title：Biochemical and biophysical research communications

authors： Cui YQ,Liu YJ,Zhang F

更新日期：2018-03-11 00:00:00

abstract：:Recent findings identify the role of proliferation of pulmonary artery smooth muscle cells (PASMCs) in pulmonary vascular remodeling. Phosphoinositide 3 kinase (PI3K) and serine/threonine kinase (Akt) proteins are expressed in vascular smooth muscle cells. In addition, phosphatase and tensin homolog deleted on chromos...

journal_title：Biochemical and biophysical research communications

authors： Luo C,Yi B,Bai L,Xia Y,Wang G,Qian G,Feng H

更新日期：2010-07-02 00:00:00

abstract：:beta-Amyloid (Abeta) is the primary protein component of senile plaques in Alzheimer's disease (AD) and has been implicated in neurotoxicity associated with the disease. Abeta aggregates readily in vitro and in vivo, and its toxicity has been linked to its aggregation state. Prevention of Abeta aggregation has been in...

journal_title：Biochemical and biophysical research communications

authors： Lee S,Carson K,Rice-Ficht A,Good T

更新日期：2006-08-25 00:00:00

abstract：:In this investigation, the activities of five enzymes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-dehydrogenase) involved in oxygen radical (OR)-detoxification and antioxidative defense were measured in conjunction with an index of lipid peroxidation in major prostatic lob...

journal_title：Biochemical and biophysical research communications

authors： Ghatak S,Ho SM

更新日期：1996-05-15 00:00:00

abstract：:Loss-of-function mutations in the DJ-1/PARK7 gene are responsible for early-onset autosomal-recessive Parkinson's disease. DJ-1 is implicated in the protection of neurons from oxidative stress by scavenging hydrogen peroxide and regulating the transcriptional activity of multiple pathways. Here, we attempted to identi...

journal_title：Biochemical and biophysical research communications

authors： Kim SJ,Park YJ,Oh YJ

更新日期：2012-05-25 00:00:00

abstract：:Induction of cyclin D1 expression is a critical feature of growth factor-induced cell proliferation in hepatocytes. To clarify the mechanisms regulating cyclin D1 gene expression, we isolated the rat cyclin D1 gene and analyzed the transcriptional regulatory elements in rat hepatoma cells and primary cultured rat hepa...

journal_title：Biochemical and biophysical research communications

authors： Moriuchi A,Ido A,Nagata Y,Nagata K,Uto H,Hasuike S,Hori T,Hirono S,Hayashi K,Tsubouchi H

更新日期：2003-01-10 00:00:00

abstract：:In order to examine the ability of an O-glycosylated serine residue preceding proline to stabilize a cis amide conformation in a fashion similar to that observed with aromatic amino acid residues, we prepared a series of glycosylated analogs of a small linear peptide which we have previous reported to contain a cis co...

journal_title：Biochemical and biophysical research communications

authors： Pao YL,Wormarld MR,Dwek RA,Lellouch AC

更新日期：1996-02-06 00:00:00

abstract：:We investigated the regulation of Hsp27 phosphorylation by protein kinase C δ (PKCδ) during etoposide-induced apoptosis. The phosphorylation of Hsp27 at Ser78 was temporally correlated with the proteolytic activation of PKCδ during apoptosis. Hsp27 phosphorylation was dependent on the activity of PKCδ since treatment ...

journal_title：Biochemical and biophysical research communications

authors： Choi JS,Oh JI,Na M,Lee SK,Joo SH

更新日期：2012-10-05 00:00:00

abstract：:A great challenge for gene therapy is to develop a high efficient gene delivery system with low toxicity. Nonviral vectors are still attractive although the current agents displayed some disadvantages (i.e., low transfection efficiency, high toxicity). To overcome the high toxicity of poly(ethylene imine) (PEI) and lo...

journal_title：Biochemical and biophysical research communications

authors： Liang B,He ML,Xiao ZP,Li Y,Chan CY,Kung HF,Shuai XT,Peng Y

更新日期：2008-03-21 00:00:00

abstract：:In this study, we calculate the unpredictable portion of amino-acid pairs, which has been developed by us over the last several years, of 1201 hemagglutinins from influenza A viruses dated from 1918 to 2004 in order to compare them with respect to subtypes, species, and years. After noticing the fluctuations of unpred...

journal_title：Biochemical and biophysical research communications

authors： Wu G,Yan S

更新日期：2005-07-22 00:00:00

abstract：:Regulation of membrane protein functions due to hydrophobic coupling with a lipid bilayer has been investigated. An energy formula describing interactions between lipid bilayer and integral ion channels with different structures, which is based on the screened Coulomb interaction approximation, has been developed. Her...

journal_title：Biochemical and biophysical research communications

authors： Ashrafuzzaman M,Tseng CY,Tuszynski JA

更新日期：2014-03-07 00:00:00

abstract：:Polyclonal antibodies (pAbs) to riboflavin carrier protein (RCP) purified from human amniotic fluid were raised in two rabbits. These pAbs apart from recognizing the parent antigen, reacted significantly with chicken RCP as well. When all the 212 octapeptides overlapping by one amino acid residue and covering the enti...

journal_title：Biochemical and biophysical research communications

authors： Subramanian S,Adiga PR

更新日期：1999-08-27 00:00:00

abstract：:We have screened a lambda gt11 cDNA library from chicken gizzard muscle with polyclonal antiserum to avian synemin. Immunopositive clones were characterized and sequenced. Computer searches identified primarily intermediate filament (IF) proteins as being homologous to the synemin clones. Synemin's sequence contained ...

journal_title：Biochemical and biophysical research communications

authors： Becker B,Bellin RM,Sernett SW,Huiatt TW,Robson RM

更新日期：1995-08-24 00:00:00

abstract：:Three mRNA species for human muscle phophofructokinase containing heterogeneous 5' untranslated sequences were identified through cDNA cloning. Type A mRNA was essentially the same as that reported previously (Nakajima, H., et al. (1987) FEBS Lett. 223, 113). Type B mRNA was considered to be the major gene product, wh...

journal_title：Biochemical and biophysical research communications

authors： Nakajima H,Yamasaki T,Noguchi T,Tanaka T,Kono N,Tarui S

更新日期：1990-01-30 00:00:00

abstract：:We investigated the expression of myocilin in the optic nerve head of porcine eyes by Western blotting and immunohistochemical staining. Myocilin was localized in the nucleus, centrosome, glial filament, mitochondria, and some parts of the cell membranes of the astrocytes. Myocilin was also detected at the edge-feet p...

journal_title：Biochemical and biophysical research communications

authors： Noda S,Mashima Y,Obazawa M,Kubota R,Oguchi Y,Kudoh J,Minoshima S,Shimizu N

更新日期：2000-10-05 00:00:00