Direct stimulation by thyrotropin-releasing hormone (TRH) of polyphosphoinositide hydrolysis in GH3 cell membranes by a guanine nucleotide-modulated mechanism.

abstract：:Perillaldehyde (PAH), one of the major oil components in Perilla frutescens, has anti-inflammatory effects. Few studies have examined the neuroprotective effect of PAH on stroke. So the aim of our study is to investigate the effect of PAH on ischemia-reperfusion-induced injury in the rat brain cortex. Middle cerebral ...

journal_title：Biochemical and biophysical research communications

authors： Xu L,Li Y,Fu Q,Ma S

更新日期：2014-11-07 00:00:00

abstract：:The present study shows for the first time that in proopiomelanocortin cells of the rat intermediate pituitary gland ANF binds to two receptor forms, with apparent molecular weights of 150K and 70K. Scatchard plots revealed specific and high affinity non-interacting sites, with a KD value of about 3 nM and a density o...

journal_title：Biochemical and biophysical research communications

authors： Lutz-Bucher B,Boudjada T,Heisler S,Pelletier G,Koch B

更新日期：1988-08-30 00:00:00

abstract：:Transthyretin (TTR) is a human disease-associated amyloidogenic protein that has been implicated in senile systemic amyloidosis (SSA) and familial amyloidotic polyneuropathy (FAP). FAP typically results in severe and early-onset disease, and the only therapy established so far is liver transplantation; thus, developin...

journal_title：Biochemical and biophysical research communications

authors： Lee KW,Lee DH,Son H,Kim YS,Park JY,Roh GS,Kim HJ,Kang SS,Cho GJ,Choi WS

更新日期：2009-10-16 00:00:00

abstract：:To study its biological functions, tumor necrosis factor precursor (proTNF) with a molecular size of 26-KDa was obtained as a recombinant protein from Escherichia coli. The recombinant proTNF was successfully accumulated in the insoluble form, corresponding to about 10-15% of total E. coli proteins. Solubilization, ge...

journal_title：Biochemical and biophysical research communications

authors： Tanabe Y,Noguchi K,Morikawa A,Mizuno D,Soma G

更新日期：1991-08-30 00:00:00

abstract：:Microinjection of inositol 1,3,4,5-tetrakisphosphate or inositol 1,4,5-trisphosphate induced distinct chloride membrane currents in defolliculated Xenopus laevis oocytes. To decide whether these Cl(-)-currents were due to the injected compounds or their metabolic products, [3H]Ins(1,3,4,5)P4 or [3H]Ins(1,4,5)P3 were i...

journal_title：Biochemical and biophysical research communications

authors： Guse AH,Gercken G,Boysen H,Schwarz JR,Meyerhof W

更新日期：1991-08-30 00:00:00

abstract：:The whole-cell patch-clamp technique has been applied to elucidate the effects of H2O2 and the SH-group reagent 2,2'-dithio-bis(5-nitropyridine) (DTBNP) on Ca2+ currents of mouse pancreatic B-cells. We also tested the effect of DTBNP on the whole-cell K+ ATP current. DTBNP (20 and 100 mumol/l) blocked both the current...

journal_title：Biochemical and biophysical research communications

authors： Krippeit-Drews P,Britsch S,Lang F,Drews G

更新日期：1994-04-29 00:00:00

abstract：AIMS:To investigate dynamic changes in the expression of HCN2, HCN4, as well as KCNE1, and KCNE2 mRNA and protein levels in ventricular cells from acute myocardial infarction (AMI) rat hearts. MAIN METHODS:An AMI model was induced by ligating the left anterior descending coronary artery (LAD) of Sprague-Dawley rats. T...

journal_title：Biochemical and biophysical research communications

authors： Xia S,Wang Y,Zhang Y,Deng SB,Du JL,Wang XC,She Q

更新日期：2010-05-07 00:00:00

abstract：:The tyrosine kinase Fms, the cell surface receptor for M-CSF and IL-34, is critical for microglial proliferation and differentiation in the brain. Recently, a number of mutations have been identified in Fms as a putative genetic cause of hereditary diffuse leukoencephalopathy with spheroids (HDLS), implying an importa...

journal_title：Biochemical and biophysical research communications

authors： Hiyoshi M,Hashimoto M,Yukihara M,Bhuyan F,Suzu S

更新日期：2013-11-01 00:00:00

abstract：:In the present study, we tested the anti-pancreatic cancer activity by AT406, a small-molecule antagonist of IAP (inhibitor of apoptosis proteins). In established (Panc-1 and Mia-PaCa-2 lines) and primary human pancreatic cancer cells, treatment of AT406 significantly inhibited cell survival and proliferation. Yet, sa...

journal_title：Biochemical and biophysical research communications

authors： Jiang Y,Meng Q,Chen B,Shen H,Yan B,Sun B

更新日期：2016-09-09 00:00:00

abstract：:To understand the regulation by thyroid hormone, 3,3',5-triiodo-L-thyronine (T3), of the synthesis of a cytosolic thyroid hormone binding protein (p58-M2) during liver regeneration, the synthesis of p58-M2 was evaluated. The synthesis of p58-M2 was measured by metabolic labeling of primary cultures derived from the re...

journal_title：Biochemical and biophysical research communications

authors： Obata T,Cheng SY

更新日期：1992-11-30 00:00:00

abstract：:In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. The ROCK-specific inhibitor, Y-27632, did not alter basal amylase secretion, whereas it potentiated CCK-...

journal_title：Biochemical and biophysical research communications

authors： Kusama K,Nozu F,Awai T,Tanaka S,Honma I,Tsunoda Y,Mitamura K

更新日期：2003-05-30 00:00:00

abstract：:While chemotherapy is an important and widely used therapeutic for cancer, it may facilitate cancer metastasis. Herein, we report that human lung cancer cells exert higher invasion and metastasis after chemotherapy. In a human lung cancer xenograft model, chemotherapy promotes the cancer invasion and metastasis in HMG...

journal_title：Biochemical and biophysical research communications

authors： Wang K,Shan S,Wang S,Gu X,Zhou X,Ren T

更新日期：2018-06-07 00:00:00

abstract：:Hepatitis B virus X protein (HBx protein), which seems to be involved in hepatocarcinogenesis, was studied for its effect on cell growth regulation. We examined the response to growth inhibition of transforming growth factor beta 1 (TGF-beta 1) in HBx gene-introduced cells. HBx gene in pRc/CMV was transfected to mink ...

journal_title：Biochemical and biophysical research communications

authors： Oshikawa O,Tamura S,Kawata S,Ito N,Tsushima H,Kiso S,Matsuda Y,Yamada A,Tamai S,Matsuzawa Y

更新日期：1996-05-24 00:00:00

abstract：:Hexokinase II protein is augmented in denervated skeletal muscle; therefore, we determined if hexokinase II gene transcription rates and mRNA levels are increased with denervation. The right hindlimb skeletal muscles of male rats were denervated while the left hindlimbs were sham operated. Seventy-two h following surg...

journal_title：Biochemical and biophysical research communications

authors： Jones JP,Roberts BR,Tapscott EB,Dohm GL

更新日期：1997-09-08 00:00:00

abstract：:Retroviral gene transfer of dominant selectable markers into hematopoietic cells can be used to select genetically modified cells in vivo or to attenuate the toxic effects of chemotherapeutic agents. We show that retroviral gene transfer of thymidylate synthase (TS) confers resistance to TS directed anticancer agents ...

journal_title：Biochemical and biophysical research communications

authors： Fantz CR,Shaw D,Moore JG,Spencer HT

更新日期：1998-02-04 00:00:00

abstract：:New antibodies reactive with a 20 KDal ribosomal protein of the large subunit were found in sera from two of eighty patients with systemic lupus erythematosus. This antigenic protein was identified as L12 by the mobility in two-dimensional gel electrophoresis. Both sera also contained anti-P activity against three aci...

journal_title：Biochemical and biophysical research communications

authors： Sato T,Uchiumi T,Kominami R,Arakawa M

更新日期：1990-10-30 00:00:00

abstract：:A number of viruses, when they bind to cells, activate intracellular signals that facilitate post-binding steps of infection. To determine if retroviruses activate intracellular signaling, we transduced HeLa cells with amphotropic retroviruses produced by TelCeB6 cells and examined cell lysates for activated Rac1. We ...

journal_title：Biochemical and biophysical research communications

authors： Krishna D,Le Doux JM

更新日期：2006-07-07 00:00:00

abstract：:In cancer treatment, specifically targeting cancer cells is important for optimal therapeutic efficacy. One strategy is to utilize a cancer specific promoter to express a cytotoxic gene or a viral gene required for replication. In this approach, the therapeutic window is dependent on the relative promoter activity in ...

journal_title：Biochemical and biophysical research communications

authors： Kurayoshi K,Shiromoto A,Ozono E,Iwanaga R,Bradford AP,Araki K,Ohtani K

更新日期：2017-01-29 00:00:00

abstract：:PRPK (p53-related protein kinase) has been reported as a novel protein kinase which binds to the tumor suppressor protein p53 and induces phosphorylation of p53 at Ser 15. To identify novel binding partners of PRPK, we performed a yeast two-hybrid screening and isolated an expressed sequence tag CGI-121 by which a 20-...

journal_title：Biochemical and biophysical research communications

authors： Miyoshi A,Kito K,Aramoto T,Abe Y,Kobayashi N,Ueda N

更新日期：2003-04-04 00:00:00

abstract：:The secretory proteins from adrenal chromaffin granules, chromogranins A, A1 and A2, were found to be excellent in vitro methyl acceptor proteins. The purified protein-carboxyl methylase (PCM) from bovine erythrocytes incorporated 660, 2540 and 7890 pmol of methyl group/10 min/mg protein in chromogranins A, A1 and A2,...

journal_title：Biochemical and biophysical research communications

authors： Veeraragavan K,Coulombe R,Gagnon C

更新日期：1988-04-29 00:00:00

abstract：:We have developed a solid matrix immunoassay to determine the binding of interleukin-2 (IL-2) to specific gangliosides. The assay establishes that recombinant human IL-2 binds to ganglioside GD(1b) but not to any other gangliosides (GM(1), GM(2), GM(3), GD(1a), GD(2), GD(3), and GT(1b)). The binding varies with the ra...

journal_title：Biochemical and biophysical research communications

authors： Ravindranath MH,Gonzales A,Soh D,Nishimoto K,Tam WY,Bilchik A,Morton DL,O'Day S

更新日期：2001-05-04 00:00:00

abstract：:We previously reported not only that chicken HIRA, a homolog of Saccharomyces cerevisiae transcriptional corepressors Hir1p and Hir2p, possesses seven WD dipeptide motifs and a LXXLL motif in its N-terminal half and C-terminal half, respectively, but also that the N-terminal and C-terminal halves, respectively, bind t...

journal_title：Biochemical and biophysical research communications

authors： Ahmad A,Takami Y,Nakayama T

更新日期：2003-12-26 00:00:00

abstract：:Cilostazol is a drug licensed for the treatment of intermittent claudication. Its main action is to elevate intracellular levels of cyclic monophosphate (cAMP) by inhibiting the activity of type III phosphodiesterase, a cAMP-degrading enzyme. The effects of cilostazol on fatty acid oxidation (FAO) are as yet unknown. ...

journal_title：Biochemical and biophysical research communications

authors： Wang B,Zhu L,Sui S,Sun C,Jiang H,Ren D

更新日期：2014-05-09 00:00:00

abstract：:The effects of cyclosporin A (CSP) on tumor necrosis factor-alpha (TNF-alpha) RNA levels and protein production in human B cells and a B cell line were studied. The ability of CSP to block induction of RNA was compared to its inhibition of protein production in response to anti-IgM, phorbol ester (PMA) and platelet-ac...

journal_title：Biochemical and biophysical research communications

authors： Smith CS,Ortega G,Parker L,Shearer WT

更新日期：1994-10-14 00:00:00

abstract：:Sodium dodecyl sulfate (SDS), a representative anionic surfactant, is a commonly used reagent in studies of the cell membrane and cell wall. However, the mechanisms through which SDS affects cellular functions have not yet been fully examined. Thus, to gain further insights into the cellular functions and responses to...

journal_title：Biochemical and biophysical research communications

authors： Zhao F,Yang J,Li J,Li Z,Lin Y,Zheng S,Liang S,Han S

更新日期：2020-06-18 00:00:00

abstract：:Disruption of the androgen receptor (AR) in male mice reduces cortical bone expansion and muscle mass during puberty and results in high bone turnover-related cancellous osteopenia. We hypothesized that voluntary wheel running during growth is able to rescue the effects of AR disruption on bone. To this end, 5-week-ol...

journal_title：Biochemical and biophysical research communications

authors： Ophoff J,Callewaert F,Venken K,De Gendt K,Ohlsson C,Gayan-Ramirez G,Decramer M,Boonen S,Bouillon R,Verhoeven G,Vanderschueren D

更新日期：2009-01-02 00:00:00

abstract：:Intracellular cholesterol transfer to mitochondria, a bottleneck of adrenal and gonadal steroidogenesis, relies on the functioning of the steroidogenic acute regulatory protein (StAR, STARD1), for which many disease-associated mutations have been described. Despite significant progress in the field, the exact mechanis...

journal_title：Biochemical and biophysical research communications

authors： Sluchanko NN,Tugaeva KV,Maksimov EG

更新日期：2017-08-05 00:00:00

abstract：:To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A)(+) RNA transport] 1 to 11, which accumulate poly(A)(+) RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5-1 mutant shows dots- or a ring-like accumul...

journal_title：Biochemical and biophysical research communications

authors： Watanabe N,Ikeda T,Mizuki F,Tani T

更新日期：2012-02-03 00:00:00

abstract：:C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor that regulates many genes involved in normal cellular events. Because CtBP2 overexpression has been implicated in various human cancers, its protein levels must be precisely regulated. Previously, we reported that CtBP1 and CtBP1-mediated transcrip...

journal_title：Biochemical and biophysical research communications

authors： Seo TW,Lee YT,Lee JS,Yoo SJ

更新日期：2020-09-17 00:00:00

abstract：AIM/BACKGROUND:CD99 participate in neutrophil infiltration after inflammatory events; however, despite the important role of inflammation in ischemic stroke, the role of CD99 in ischemic stroke remains unclear. METHOD:In the present study, we detected the protein expression of CD99, ICAM-1, and CD31 (PECAM-1) in oxyge...

journal_title：Biochemical and biophysical research communications

authors： Hu T,Sun R,Huang F,Liu X,Duan Z,Ye R,Li Y,Xiao L,Guo Z,Liu Q,Zhu W

更新日期：2020-06-04 00:00:00