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Molecular cloning of the human gene encoding lanosterol synthase from a liver cDNA library.

Abstract：

:Lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7] catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol in the reaction that forms the sterol nucleus. We report herein the cloning and characterization of the human gene (OSC) encoding lanosterol synthase, a predicted 83 kDa protein of 732 amino acids. The deduced amino acid sequence is 36-40% identical to known yeast and plant homologues and 83% identical to Rattus norvegicus lanosterol synthase. The new gene was shown to encode lanosterol synthase. The yeast lanosterol synthase deficient mutant SMY8 was complemented by the human gene, and a cell-free homogenate of SMY8 expressing the human gene was shown to convert 2,3-oxidosqualene to lanosterol.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Baker CH,Matsuda SP,Liu DR,Corey EJ

doi

10.1006/bbrc.1995.2110

subject

Has Abstract

pub_date

1995-08-04 00:00:00

pages

154-60

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(85)72110-9

journal_volume

213

pub_type

杂志文章

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