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Cloning, expression, and in vitro activity of human endostatin.

Abstract：

:Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. It specifically inhibited the proliferation and migration of endothelial cells. In addition, we report for the first time that endostatin caused G1 arrest of endothelial cells. Also, we show that endostatin treatment resulted in apoptosis of HUVE and HMVE cells and that all of these effects do not occur in nonendothelial cells. Collectively, these findings demonstrate the expression of a biologically active form of human endostatin in yeast and provide important mechanistic insight into endostatin action on endothelial cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Dhanabal M,Volk R,Ramchandran R,Simons M,Sukhatme VP

doi

10.1006/bbrc.1999.0595

subject

Has Abstract

pub_date

1999-05-10 00:00:00

pages

345-52

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(99)90595-8

journal_volume

258

pub_type

杂志文章

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