Amino acid sequence surrounding the chondroitin sulfate attachment site of thrombomodulin regulates chondroitin polymerization.


:Thrombomodulin (TM) is a cell-surface glycoprotein and a critical mediator of endothelial anticoagulant function. TM exists as both a chondroitin sulfate (CS) proteoglycan (PG) form and a non-PG form lacking a CS chain (α-TM); therefore, TM can be described as a part-time PG. Previously, we reported that α-TM bears an immature, truncated linkage tetrasaccharide structure (GlcAβ1-3Galβ1-3Galβ1-4Xyl). However, the biosynthetic mechanism to generate part-time PGs remains unclear. In this study, we used several mutants to demonstrate that the amino acid sequence surrounding the CS attachment site influences the efficiency of chondroitin polymerization. In particular, the presence of acidic residues surrounding the CS attachment site was indispensable for the elongation of CS. In addition, mutants defective in CS elongation did not exhibit anti-coagulant activity, as in the case with α-TM. Together, these data support a model for CS chain assembly in which specific core protein determinants are recognized by a key biosynthetic enzyme involved in chondroitin polymerization.


Izumikawa T,Kitagawa H




Has Abstract


2015-05-01 00:00:00














  • 5-HPETE is a potent inhibitor of neuronal Na+, K(+)-ATPase activity.

    abstract::The effects of 1 microM concentrations of arachidonic acid hydroperoxide (HPETES) products of 5-, 12- and 15-lipoxygenase on Na+, K(+)-ATPase activity were investigated in synaptosomal membrane preparations from rat cerebral cortex. 5-HPETE inhibited Na+, K(+)-ATPase activity by up to 67 %. In contrast, 12-HPETE and 1...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Foley TD

    更新日期:1997-06-18 00:00:00

  • Simultaneous measurement of intracellular pH, calcium, and tension in rat mesenteric vessels: effects of extracellular pH.

    abstract::In rat mesenteric vessels changes in external pH (pHo) alter tension. Changes in intracellular pH (pHi) and calcium ([Ca2+]i) have both been suggested to underlie the tension changes. As no simultaneous measurements of pHi and [Ca2+]i had been made, however, the relative importance and temporal relationship between pH...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Austin C,Dilly K,Eisner D,Wray S

    更新日期:1996-05-15 00:00:00

  • Honokiol induces caspase-independent paraptosis via reactive oxygen species production that is accompanied by apoptosis in leukemia cells.

    abstract::Our previous report has shown that honokiol (HNK), a constituent of Magnolia officinalis, induces a novel form of non-apoptotic programmed cell death in human leukemia NB4 and K562 cells. In this study, we further explored the relationship between the cell death pathway and cytoplasmic vacuolization and studied the un...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang Y,Zhu X,Yang Z,Zhao X

    更新日期:2013-01-18 00:00:00

  • Tumor necrosis factor superfamily member LIGHT induces epithelial-mesenchymal transition in A549 human alveolar epithelial cells.

    abstract::Fibrosis is an abnormal response to organ injury, characterized by accumulation of activated fibroblasts at the sites of injury. Fibroblasts arise from several sources, including resident fibroblasts and circulating fibrocytes that infiltrate organ tissue. Recently, epithelial-mesenchymal transition (EMT) has been rec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mikami Y,Yamauchi Y,Horie M,Kase M,Jo T,Takizawa H,Kohyama T,Nagase T

    更新日期:2012-11-30 00:00:00

  • Inhibition of the formation of the neurotoxin 5-S-cysteinyl-dopamine by polyphenols.

    abstract::The death of nigral neurons in Parkinson's disease is thought to involve the formation of the endogenous neurotoxin, 5-S-cysteinyl-dopamine. In the present study, we show that the polyphenols, (+)-catechin and caffeic acid, which contain a catechol moiety, inhibit tyrosinase-induced formation of 5-S-cysteinyl-dopamine...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Vauzour D,Vafeiadou K,Spencer JP

    更新日期:2007-10-19 00:00:00

  • Direct evidence that the brain reward system is involved in the control of scratching behaviors induced by acute and chronic itch.

    abstract::In the present study, we demonstrated that there is a direct relationship between scratching behaviors induced by itch and functional changes in the brain reward system. Using a conditional place preference test, the rewarding effect was clearly evoked by scratching under both acute and chronic itch stimuli. The induc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Setsu T,Hamada Y,Oikawa D,Mori T,Ishiuji Y,Sato D,Narita M,Miyazaki S,Furuta E,Suda Y,Sakai H,Ochiya T,Tezuka H,Iseki M,Inada E,Yamanaka A,Kuzumaki N,Narita M

    更新日期:2021-01-01 00:00:00

  • Localization of osteogenic protein-1 (bone morphogenetic protein-7) during human embryonic development: high affinity binding to basement membranes.

    abstract::Osteogenic protein-1 (OP-1) is a member of the bone morphogenetic protein subfamily of the transforming growth factor-beta (TGF-beta) superfamily. Since members of the TGF-beta superfamily have a role in tissue development the distribution of OP-1 in developing human embryos (gestational age 5-14 weeks) was examined b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Vukicevic S,Latin V,Chen P,Batorsky R,Reddi AH,Sampath TK

    更新日期:1994-01-28 00:00:00

  • Carnosine protects proteins against methylglyoxal-mediated modifications.

    abstract::Methylglyoxal (MG) (pyruvaldehyde) is an endogenous metabolite which is present in increased concentrations in diabetics and implicated in formation of advanced glycosylation end-products (AGEs) and secondary diabetic complications. Carnosine (beta-alanyl-L-histidine) is normally present in long-lived tissues at conce...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hipkiss AR,Chana H

    更新日期:1998-07-09 00:00:00

  • Differences in the active site environment of Aspergillus ficuum phytases.

    abstract::While Aspergillus ficuum phytaseA (phyA) was rapidly inactivated by 1,2-cyclohexanedione and phenylglyoxal, both specific modifiers of arginine, phytaseB (phyB) showed a markedly different behavior. First, phyB was totally insensitive to 1,2-cyclohexanedione even in the presence of 0.2 M guanidinium hydrochloride; sec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ullah AH,Sethumadhavan K

    更新日期:1998-02-13 00:00:00

  • Parathyroid hormone inhibition of Na(+)/H(+) exchanger 3 transcription: Intracellular signaling pathways and transcription factor expression.

    abstract::The main transport mechanism of reabsorption of sodium bicarbonate and fluid in the renal proximal tubules involves Na(+)/H(+) exchanger 3 (NHE3), which is acutely and chronically downregulated by parathyroid hormone (PTH). Although PTH is known to exert an inhibitory effect on NHE3 expression and transcription, the m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Neri EA,Bezerra CN,Queiroz-Leite GD,Polidoro JZ,Rebouças NA

    更新日期:2015-06-12 00:00:00

  • COBRA1 inhibits AP-1 transcriptional activity in transfected cells.

    abstract::Mutations in the breast cancer susceptibility gene (BRCA1) account for a significant proportion of hereditary breast and ovarian cancers. Cofactor of BRCA1 (COBRA1) was isolated as a BRCA1-interacting protein and exhibited a similar chromatin reorganizing activity to that of BRCA1. However, the biological role of COBR...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhong H,Zhu J,Zhang H,Ding L,Sun Y,Huang C,Ye Q

    更新日期:2004-12-10 00:00:00

  • Identification of parathyroid hormone-regulated proteins in mouse bone marrow cells by proteomics.

    abstract::The ability of parathyroid hormone (PTH) to enhance bone formation has recently been exploited in the treatment of osteoporosis. Several studies have suggested that the activation of bone marrow stromal cells could be preceded to show the anabolic effect of PTH on bone formation, but little is known of PTH-regulated p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim SH,Jun S,Jang HS,Lim SK

    更新日期:2005-05-06 00:00:00

  • Dispersity of des-L X Val7-D X Val8-Gramicidin A single channel conductances argues for different side chain orientations as basis.

    abstract::Planar bilayer studies are reported on the channel activity of des-L X Val7-D X Val8-Gramicidin A. This analog is designed to provide more long-lived side chain distributions involving the Trp residues than occur with Gramicidin A. The carbonyls of these residues coordinate the permeant cation and the energetics of th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Urry DW,Alonso-Romanowski S,Venkatachalam CM,Harris RD,Prasad KU

    更新日期:1984-02-14 00:00:00

  • Blood brain barrier breakdown in brain edema following cold injury is mediated by microvascular polyamines.

    abstract::A focal freeze injury to rat cerebral cortex induces an early (less than 5 min) increase in brain ornithine decarboxylase activity and an accumulation of polyamines involving cerebral microvessels. This polyamine synthesis correlates with the abnormal increase in microvascular permeability, monitored by uptake of Evan...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Koenig H,Goldstone AD,Lu CY

    更新日期:1983-11-15 00:00:00

  • Targeting Hsc70-based autophagy to eliminate amyloid β oligomers.

    abstract::Amyloid β (Aβ) oligomers may be a real culprit in the pathogenesis of Alzheimer's disease (AD); therefore, the elimination of these toxic oligomers may be of great significance for AD therapy. Autophagy is the catabolic process by which lysosomes degrade cytosolic components, and heat shock cognate 70 kDa protein (Hsc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dou J,Su P,Xu C,Wen Z,Mao Z,Li W

    更新日期:2020-04-16 00:00:00

  • Differential expression of the transcription factor NF-kappaB during human mononuclear phagocyte differentiation to macrophages and dendritic cells.

    abstract::An important role for the Rel/NF-kappaB family of transcription factors in the differentiation process of dendritic cells (DC) and macrophages (MAC) was recently suggested by a number of mouse knockout studies but only little information is available for defined populations of human cells. To investigate the role of i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ammon C,Mondal K,Andreesen R,Krause SW

    更新日期:2000-02-05 00:00:00

  • miR-199b-5p modulates BMSC osteogenesis via suppressing GSK-3β/β-catenin signaling pathway.

    abstract::miR-199b-5p is up-regulated significantly during the osteogenesis process in human bone marrow stromal cells (BMSCs). Inhibiting miR-199b-5p notably reduces while over-expressing miR-199b-5p promotes the BMSCs osteoblast differentiation, suggested by the alternations of osteogenic genes expression, ALP activity and th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhao R,Li Y,Lin Z,Wan J,Xu C,Zeng Y,Zhu Y

    更新日期:2016-09-02 00:00:00

  • Adenosine induces apoptosis through TNFR1/RIPK1/P38 axis in colon cancer cells.

    abstract::Adenosine, a metabolite of ATP, ubiquitously exists in a wide range of organs and tissues. We previously reported that adenosine was implicated in apoptosis in many cancer cells by extrinsic and/or intrinsic pathways. Here, we found that adenosine suppresses the cell growth by induction of apoptosis of human colonic c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yu S,Hou D,Chen P,Zhang Q,Lv B,Ma Y,Liu F,Liu H,Song EJ,Yang D,Liu J

    更新日期:2015-05-08 00:00:00

  • miR-22 regulates starvation-induced autophagy and apoptosis in cardiomyocytes by targeting p38α.

    abstract::microRNAs (miRNAs) are short noncoding RNAs that function in RNA silencing and post-transcriptional regulation of gene expression. They play critical regulatory roles in many cardiovascular diseases, including ischemia-induced cardiac injury. Here, we report microRNA-22, highly expressed in the heart, can protect card...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li G,Wang G,Ma L,Guo J,Song J,Ma L,Zhao X

    更新日期:2016-09-23 00:00:00

  • Transcriptional activation of SHP by PPAR-gamma in liver.

    abstract::The mechanism of how PPARgamma decrease gluconeogenic gene expressions in liver is still unclear. Since PPARgamma is a transcriptional activator, it requires a mediator to decrease the transcription of gluconeogenic genes. Recently, SHP has been shown to mediate the bile acid-dependent down regulation of gluconeogenic...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim HI,Koh YK,Kim TH,Kwon SK,Im SS,Choi HS,Kim KS,Ahn YH

    更新日期:2007-08-24 00:00:00

  • The subcellular location of the yeast Saccharomyces cerevisiae homologue of the protein defective in the juvenile form of Batten disease.

    abstract::The mutation responsible for the juvenile form of Batten disease was mapped to a single gene, Cln3 (T. J. Lerner et al. (1995) Cell 82:949-957). Yeast Saccharomyces cerevisiae has an open reading frame, BTN1 (YHC3), that encodes the putative homologue of Cln3p. Primary structure comparison indicates that the human Cln...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Croopnick JB,Choi HC,Mueller DM

    更新日期:1998-09-18 00:00:00

  • Frequency and Markov chain analysis of amino-acid sequences of human glutathione reductase.

    abstract::The amino-acid sequence of human glutathione reductase was measured according to two- and three-amino-acid sequences. The measured frequency and probability were compared with predicted frequency and probability. Of 477 two-amino-acid sequences in human glutathione reductase, 176 (36.897%) and 90 (18.868%) sequences c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wu G

    更新日期:2000-02-24 00:00:00

  • Sulfonate salts of amino acids: novel inhibitors of the serine proteinases.

    abstract::A series of amino acid-derived sulfonate salts have been synthesized. They were found to inactivate efficiently and selectively human leukocyte elastase. The sulfonate salts of the methyl esters of L-norleucine, L-norvaline and L-valine were the most potent. The enzyme is inactivated irreversibly with concomitant rele...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Groutas WC,Brubaker MJ,Zandler ME,Stanga MA,Huang TL,Castrisos JC,Crowley JP

    更新日期:1985-04-16 00:00:00

  • WISP-2 is a secreted protein and can be a marker of estrogen exposure in MCF-7 cells.

    abstract::As many structurally diverse chemicals have been reported to function as estrogens, evaluations for estrogenicity of compounds are of widespread concern. Recently, we identified WISP-2 (Wnt-1 inducible signaling pathway protein 2) as a novel estrogen-inducible gene in human breast cancer cells. In this study, we exami...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Inadera H,Dong HY,Matsushima K

    更新日期:2002-06-14 00:00:00

  • Insulin-like growth factor-binding protein-3 inhibits IGF-1-induced proliferation of human hepatocellular carcinoma cells by controlling bFGF and PDGF autocrine/paracrine loops.

    abstract::Basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) produced by hepatocellular carcinoma (HCC) cells are responsible for the growth of HCC cells. Accumulating evidence shows that insulin-like growth factor-binding protein-3 (IGFBP-3) suppresses HCC cell proliferation in both IGF-dependent a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ma Y,Han CC,Li Y,Wang Y,Wei W

    更新日期:2016-09-16 00:00:00

  • Ciliary smoothened-mediated noncanonical hedgehog signaling promotes tubulin acetylation.

    abstract::Hedgehog (Hh) signaling plays key roles in animal development and tissue homeostasis. Binding of the secreted ligand to its Ptch1 receptor triggers Hh signaling through distinct canonical or noncanonical signaling pathways. Canonical Hh signaling leads to the activation of Gli transcription factors to induce Hh target...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee H,Ko HW

    更新日期:2016-11-25 00:00:00

  • Rat insulinoma-derived pancreatic beta-cells express a functional leptin receptor that mediates a proliferative response.

    abstract::In addition to its interaction at hypothalamic sites to affect feeding and energy expenditure, leptin has been shown to exhibit a proliferative response in erythropoietic cells. The functional leptin receptor is also present in pancreatic islets and we now demonstrate that a commonly used clonal insulin secreting beta...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Islam MS,Morton NM,Hansson A,Emilsson V

    更新日期:1997-09-29 00:00:00

  • The mode of chaperoning of dithiothreitol-denatured alpha-lactalbumin by alpha-crystallin.

    abstract::Molecular chaperones prevent the aggregation of partially folded or misfolded forms of protein. alpha-crystallin performs such a function in the ocular lens. To gain insight into the mechanism of the anti-aggregation activity of alpha-crystallin, we performed dynamic light scattering (DLS) measurements investigating i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bettelheim FA,Ansari R,Cheng QF,Zigler JS Jr

    更新日期:1999-08-02 00:00:00

  • miR-503 suppresses metastasis of hepatocellular carcinoma cell by targeting PRMT1.

    abstract::Accumulating evidence indicates that microRNAs function as oncogenes or tumor suppressor genes in human cancer. MiR-503 is deregulated in various human cancers and has been associated with hepatocellular carcinoma (HCC) progression. However, the underlying mechanisms of miR-503 involvement in the development and progr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li B,Liu L,Li X,Wu L

    更新日期:2015-09-04 00:00:00

  • Overexpression of human WNK1 increases paracellular chloride permeability and phosphorylation of claudin-4 in MDCKII cells.

    abstract::Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, hyperkalemia, and hyperchloremic acidosis, is reportedly due to mutations in WNK1 and WNK4 kinase genes. However, the pathogenesis of the disease remains unknown. Mutations in the WNK1 gene are the deletions in the f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ohta A,Yang SS,Rai T,Chiga M,Sasaki S,Uchida S

    更新日期:2006-10-20 00:00:00