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Engineering of Kex2 variants exhibiting altered substrate specificity.

Abstract：

:Engineering of secreted protease variants exhibiting altered substrate specificity is a challenging task because effective screening methods for the desired property are not available yet. In this study, we sought to obtain variants of Kex2, a yeast Golgi protease, which exhibit altered P2 specificity. We first randomly mutated three Asp residues (D176, D210, and D211) that constitute the S2 pocket of Kex2 and then isolated from the resulting library Kex2 variants that preferred substrates with Met (poorly preferred by wild type Kex2) at the P2 position using a yeast-based screening method. The Kex2 variants isolated from this initial screening were further tested against various substrate sequences. Four out of the 16 isolated Kex2 variants showed greater preference for Met than for Lys (preferred by the wild-type Kex2) at the P2 position. We therefore suggest that our method might serve as an efficient tool for engineering and directing the evolution of secreted proteases.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Han HE,Rho SH,Lee YJ,Park WJ

doi

10.1016/j.bbrc.2005.09.158

subject

Has Abstract

pub_date

2005-12-02 00:00:00

pages

1102-6

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)02170-4

journal_volume

337

pub_type

杂志文章

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