Analysis of human terminal deoxynucleotidyl transferase cDNA expressible in mammalian cells.

abstract：:We present a comprehensive comparison of the anatomical distributions of the cloned mu, delta and kappa opioid receptor mRNA in rat brain. Northern blot analysis revealed that mRNA species encoding the three receptors differed in size and were differentially localized in brain regions. In peripheral tissues analyzed, ...

journal_title：Biochemical and biophysical research communications

authors： George SR,Zastawny RL,Briones-Urbina R,Cheng R,Nguyen T,Heiber M,Kouvelas A,Chan AS,O'Dowd BF

更新日期：1994-12-15 00:00:00

abstract：:CREB3 (Luman) is a family member of ER resident transcription factors, which are cleaved upon the induction of ER stress. Their N-terminal fragments shuttle into the nucleus where they regulate the transcription of target genes. Here, we found that human CREB3 is phosphorylated within its transcription activation doma...

journal_title：Biochemical and biophysical research communications

authors： Schmitt BM,Ampofo E,Stumpf H,Montenarh M,Götz C

更新日期：2020-03-12 00:00:00

abstract：:Streptococcus mutans secretes a sucrose-independent branalphang enzyme that utilizes isomaltosaccharides as donors for branalphang formation on dextran. Although the branching enzyme is necessary for the formation of extracellular polysaccharide complexes, the source of the donor for the enzyme is unknown. In this stu...

journal_title：Biochemical and biophysical research communications

authors： Yamashita Y,Hanada N,Takehara T

更新日期：1988-01-29 00:00:00

abstract：:Fibrosis is an abnormal response to organ injury, characterized by accumulation of activated fibroblasts at the sites of injury. Fibroblasts arise from several sources, including resident fibroblasts and circulating fibrocytes that infiltrate organ tissue. Recently, epithelial-mesenchymal transition (EMT) has been rec...

journal_title：Biochemical and biophysical research communications

authors： Mikami Y,Yamauchi Y,Horie M,Kase M,Jo T,Takizawa H,Kohyama T,Nagase T

更新日期：2012-11-30 00:00:00

abstract：:Corticotropin-releasing factor (CRF) increased intracellular Ca2+ concentration in single astrocytes. The effect in increasing intracellular Ca2+ was not observed in Ca2(+)-free solution. Furthermore, CRF at concentrations more than 10 nM stimulated 45Ca2+ uptake in cultured rat astrocytes. The action was blocked by a...

journal_title：Biochemical and biophysical research communications

authors： Takuma K,Matsuda T,Yoshikawa T,Kitanaka J,Gotoh M,Hayata K,Baba A

更新日期：1994-03-30 00:00:00

abstract：:Estrogen-Estrogen Receptor alpha (ERα) belongs to one of the most important signaling pathways controlling breast tissue development and progression of breast cancer. ERRF was recently identified as a candidate breast cancer associated protein and showed positive association with ERα status in clinical samples and cel...

journal_title：Biochemical and biophysical research communications

authors： Luo A,Zhang X

更新日期：2016-05-27 00:00:00

abstract：:The previously reported substrate-heme complex approach is used to study the binding of type II inhibitors of the enzyme cholesterol side chain cleavage (CSCC), a cytochrome P-450 dependent enzyme involved in the oxidative cleaveage of the C(20)-C(22) bond of cholesterol. Using the derived model, we have rationalised ...

journal_title：Biochemical and biophysical research communications

authors： Ahmed S

更新日期：2000-08-11 00:00:00

abstract：:Upon centrifugation of gently lysed T. cruzi cells through a sucrose gradient, a free DNA fraction was shown to contain catenated dimers and knotted DNA structures. Southern hybridization and electron microscopic studies indicated that both of these structures derived from minicircle DNA, the major component of T. cru...

journal_title：Biochemical and biophysical research communications

authors： Valenzuela MS,Bardhan S,Krishnamani MR,Siddiqui KA

更新日期：1991-01-31 00:00:00

abstract：:Glutamate receptor delta2 (GluD2) is selectively expressed on the postsynaptic spines at parallel-fiber (PF)-Purkinje neuron (PN) synapses. GluD2 knockout mice show a reduced number of PF-PN synapses, suggesting that GluD2 is involved in synapse formation. Recent studies revealed that GluD2 induces presynaptic differe...

journal_title：Biochemical and biophysical research communications

authors： Kuroyanagi T,Hirano T

更新日期：2010-07-30 00:00:00

abstract：:The kallikrein inhibitor found in Bauhinia bauhinioides seeds (BbKI) differs from classical Kunitz plant inhibitors in the lack of disulfide bridges in its structure [Biochim. Biophys. Acta 1477 (2000) 64-74]. In this study, we examined whether structural properties may be involved in inhibitory specificity and, if so...

journal_title：Biochemical and biophysical research communications

authors： Cagliari CI,De Caroli FP,Nakahata AM,Araújo MS,Nakaie CR,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2003-11-07 00:00:00

abstract：:NADPH oxidases (NOX) - derived reactive oxygen species (ROS) contribute to oxidative injury in hypoxia-induced pulmonary arterial hypertension. This study aims to evaluate the status of NOX in endothelial progenitor cells (EPCs) under hypoxic condition and to determine whether NOX inhibitors could attenuate hypoxia-in...

journal_title：Biochemical and biophysical research communications

authors： Liu B,Ren KD,Peng JJ,Li T,Luo XJ,Fan C,Yang JF,Peng J

更新日期：2017-01-22 00:00:00

abstract：:The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) was investigated at pH 6.2 and 21.0 degrees C. The results are consistent with the minimum three-step catalytic mechanism. The acylation step is rate limiting for human...

journal_title：Biochemical and biophysical research communications

authors： Balliano G,Milla P,Giordano C,Gallina C,Coletta M,Menegatti E,Rizzi M,Bolognesi M,Ascenzi P

更新日期：1996-08-14 00:00:00

abstract：:Preincubation of cultured rat pituitary cells with 10 micrograms/ml of either wheat germ agglutinin (WGA) or concanavalin A inhibited LH release stimulated with GnRH (0.5 nM) by 55% and 40%, respectively. WGA-inhibition of LH release stimulated by GnRH was dose-dependent, reaching a plateau of 75% inhibition at 50 mic...

journal_title：Biochemical and biophysical research communications

authors： Schvartz I,Hazum E

更新日期：1986-10-30 00:00:00

abstract：:Activating transcription factor 6α (ATF6α) as a transducer in unfolded protein response (UPR), plays an important role in liver glucose metabolism and insulin resistance. Thus, targeting ATF6α activation has been proposed to be a potential strategy for anti-T2DM drug discovery. Here, we determined that small molecule ...

journal_title：Biochemical and biophysical research communications

authors： Zhou T,Cheng Y,Yan W,Shi X,Xu X,Zhou J,Li J,Chen J,Shen X

更新日期：2018-05-23 00:00:00

abstract：:The mRNA coding for thyroglobulin in cattle homozygous for an autosomal recessive defect of thyroglobulin synthesis was investigated using a recombinant plasmid containing bovine mRNA coding sequence. Total RNA preparations from goiter contained one third of the thyroglobulin mRNA sequences found in normal thyroid tis...

journal_title：Biochemical and biophysical research communications

authors： Ricketts MH,Vandenplas S,van der Walt M,van Jaarsveld PP,Bester AJ,Boyd CD

更新日期：1985-01-16 00:00:00

abstract：:Phage displayed peptide library was used to select tumor necrosis factor alpha (TNFalpha) binding peptides. After three sequential rounds of biopanning, some linear TNFalpha-binding peptides were identified from a 12-mer peptide library. A consensus sequence (L/M)HEL(Y/F)(L/M)X(W/Y/F), where X might be variable residu...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Zheng L,Zhao A,Gao B,Liu NL,Wang F,Dong J,Xin ZT,Shao NS,Wang HX,Xue YN

更新日期：2003-10-31 00:00:00

abstract：:Fanconi anemia (FA) is a genetic disorder characterized by congenital malfunction, bone marrow failure and hypersensitivity to DNA damage. FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fanc...

journal_title：Biochemical and biophysical research communications

authors： Yang Q,Xie H,Zhong Y,Li D,Ke X,Ying H,Yu B,Zhang T

更新日期：2019-06-30 00:00:00

abstract：:A Y-box binding protein ChYB-1 was discovered as a ChHsc70 promoter-associated protein in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. The overexpression of ChYB-1 in heterologous HEK293T cells led to clear enhancement of ChHsc70 promoter expression, while ChYB-1 depletion cor...

journal_title：Biochemical and biophysical research communications

authors： Liu H,Xu D,Cui M,Hou T,Zhang Q

更新日期：2018-01-22 00:00:00

abstract：:Palmar fibromatosis is a benign fibroproliferative tumor of unknown etiology, with a high rate of recurrence after excision. The offending cells of palmar fibromatosis are myofibroblasts and the cellular origin of other myofibroblasts has previously been reported to be the bone marrow. However, further clarification o...

journal_title：Biochemical and biophysical research communications

authors： Wang JP,Hui YJ,Wang ST,Huang YC,Chiang ER,Liu CL,Chen TH,Hung SC

更新日期：2011-05-06 00:00:00

abstract：:Interleukin-4 (IL-4) is a pleiotropic immunomodulatory cytokine produced mainly by T lymphocyte. It is known that many kinds of non-haematopoietic cells, as well as immune cells and haematopoietic cells, express IL-4 receptors. Recently, we found that IL-4 increased hydroxyproline and osteocalcin accumulation and caus...

journal_title：Biochemical and biophysical research communications

authors： Ishibashi H,Karube S,Yamakawa A,Koshihara Y

更新日期：1995-06-26 00:00:00

abstract：:Activities of three kinases, phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and diacylglycerol (DG) kinases, and phospholipase C were measured in erythrocyte ghosts from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). PI kinase activity was significantly higher in SH...

journal_title：Biochemical and biophysical research communications

authors： Kato H,Takenawa T

更新日期：1987-08-14 00:00:00

abstract：:Previous studies showed that biotin enhanced glucose-induced insulin secretion. Changes in the cytosolic ATP/ADP ratio in the pancreatic islets participate in the regulation of insulin secretion by glucose. In the present study we investigated whether biotin regulates the cytosolic ATP/ADP ratio in glucose-stimulated ...

journal_title：Biochemical and biophysical research communications

authors： Sone H,Sasaki Y,Komai M,Toyomizu M,Kagawa Y,Furukawa Y

更新日期：2004-02-13 00:00:00

abstract：:RANTES is a cytokine produced by activated T-lymphocytes that has been shown to exert chemotactic activity for memory-type CD4 T-lymphocytes and eosinophils. In this study, RANTES caused directional migration of human mast cells. When compared to other potential chemoattractants of the same cells, RANTES was found to ...

journal_title：Biochemical and biophysical research communications

authors： Mattoli S,Ackerman V,Vittori E,Marini M

更新日期：1995-04-06 00:00:00

abstract：:An in organello footprinting approach has been used to probe a protein-DNA interaction of a nuclear coded 25 kDa protein, previously isolated in our laboratory, that binds "in vitro" a region within the ND2 gene, located upstream of the Ori-L. Footprinting studies with the purine-modifying reagent dimethyl sulfate and...

journal_title：Biochemical and biophysical research communications

authors： Cingolani G,Capaccio L,D'Elia D,Gadaleta G

更新日期：1997-02-24 00:00:00

abstract：:Cell cultures of neonatal rat cerebral cortex in a serum-free medium were used to investigate a lack of neuronotrophic factors in Alzheimer's disease (AD) brain. A few neurons survived in the absence of brain extract. The addition of normal brain extract resulted in a 2.5-fold increase in neuronal survival. AD brain e...

journal_title：Biochemical and biophysical research communications

authors： Uchida Y,Ihara Y,Tomonaga M

更新日期：1988-02-15 00:00:00

abstract：:A DNA endonuclease complex which recognizes predominantly pyrimidine dimers in UVC irradiated DNA has been isolated from the chromatin of normal human and xeroderma pigmentosum, complementation group D (XPD) lymphoblastoid cells. The activity of the normal complex on UVC irradiated DNA was increased approximately 2.5 ...

journal_title：Biochemical and biophysical research communications

authors： Parrish DD,Feng X,Lambert MW

更新日期：1992-12-15 00:00:00

abstract：:Incubation of rabbit aortic rings with interleukin-1 (100 U/ml) in vitro led to a depressed contractile response to norepinephrine, whether the endothelium was present or not. In both cases norepinephrine-induced contraction was restored in the presence of NG-methyl-L-arginine (300 microM), an inhibitor of nitric oxid...

journal_title：Biochemical and biophysical research communications

authors： Robert R,Chapelain B,Jean T,Néliat G

更新日期：1992-01-31 00:00:00

abstract：:S-modulin is a 26 kDa protein that regulates light sensitivity of cGMP phosphodiesterase in a Ca(2+)-dependent manner in frog rod outer segments (ROSs). In the present study, we purified S-modulin by taking advantage of a hydrophobic interaction between Phenyl Sepharose and S-modulin at high Ca2+ concentrations. The y...

journal_title：Biochemical and biophysical research communications

authors： Kawamura S,Takamatsu K,Kitamura K

更新日期：1992-07-15 00:00:00

abstract：:The anthrax lethal factor (LF) is a Zn(2+)-endopeptidase specific for mitogen-activated protein kinase kinases (MAPKKs), which are cleaved within their N-terminal region. Much line of effort was carried out to elucidate the catalytic activity of LF for designing the inhibitor and to understand the cellular mechanism o...

journal_title：Biochemical and biophysical research communications

authors： Kim J,Chai YG,Yoon MY

更新日期：2004-01-02 00:00:00

abstract：:Vibrio cholerae cytolysin (VCC), a β-barrel pore-forming toxin (β-PFT), induces killing of the target eukaryotic cells by forming heptameric transmembrane β-barrel pores. Consistent with the β-PFT mode of action, binding of the VCC toxin monomers with the target cell membrane triggers formation of pre-pore oligomeric ...

journal_title：Biochemical and biophysical research communications

authors： Paul K,Chattopadhyay K

更新日期：2014-01-03 00:00:00