Catenated dimers and knotted DNA structures: putative intermediates in the replication of T. cruzi kinetoplast minicircle DNA.

Abstract:

:Upon centrifugation of gently lysed T. cruzi cells through a sucrose gradient, a free DNA fraction was shown to contain catenated dimers and knotted DNA structures. Southern hybridization and electron microscopic studies indicated that both of these structures derived from minicircle DNA, the major component of T. cruzi kinetoplast DNA. Partial denaturation analysis of a random population of catenated dimers suggests that these structures may have arisen from a late stage in the replication of minicircle DNA. On the other hand, the T. cruzi knotted minicircles we have isolated appear to be very similar to trefoil structures recently reported and implicated as replicative intermediates in two other trypanosoma species.

authors

Valenzuela MS,Bardhan S,Krishnamani MR,Siddiqui KA

doi

10.1016/0006-291x(91)91512-b

subject

Has Abstract

pub_date

1991-01-31 00:00:00

pages

958-68

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91512-B

journal_volume

174

pub_type

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