当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Degradation of microvascular brain endothelial cell beta-catenin after co-culture with activated neutrophils from patients undergoing cardiac surgery with prolonged cardiopulmonary bypass.

Degradation of microvascular brain endothelial cell beta-catenin after co-culture with activated neutrophils from patients undergoing cardiac surgery with prolonged cardiopulmonary bypass.

Abstract：

:The adhesion of highly activated neutrophils to cerebral microvascular endothelial cells (MVECs) may contribute to disruption and hyperpermeability of the blood-brain barrier (BBB) after cardiac surgery with prolonged cardiopulmonary bypass (CPB). A correlation between CPB duration and neutrophil-mediated BBB damage has not been investigated on the cellular level yet. Therefore, we studied the effects of neutrophils from cardiac surgery patients with CPB time <80 min (group I; n=8) and >80 min (group II; n=8) on the integrity of cultured porcine MVEC. Ex vivo, neutrophils of group II but not of group I significantly degraded the zonula adherens molecule beta-catenin whereas VE-cadherin and occludin were not modified. The transendothelial electric resistance as a measure for the integrity of the endothelial monolayers was reduced over time in both groups. In conclusion, prolonged CPB time entails neutrophil-mediated decrease in MVEC beta-catenin expression, and thus may be an important trigger for BBB disruption.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Schuller AM,Windolf J,Blaheta R,Cinatl J,Kreuter J,Wimmer-Greinecker G,Moritz A,Scholz M

doi

10.1016/j.bbrc.2005.02.019

subject

Has Abstract

pub_date

2005-04-08 00:00:00

pages

616-23

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)00250-0

journal_volume

329

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Titration of variant DNA sequences differing by a single point-mutation by selective dot-blot hybridization with synthetic oligonucleotides.

    abstract：:A dot-blot hybridization procedure with synthetic oligonucleotide probes is reported, which allows the quantitative titration in genomic DNA of variant forms of repeated genes differing by a single nucleotide change. It involves the utilization of a pair of 22-base long oligonucleotides matching the two variant sequen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92242-0

    authors： Nicoloso M,Liang HQ,Bachellerie JP

    更新日期：1989-03-31 00:00:00

  • Mutants of smooth muscle myosin light chain kinase at tryptophan 800.

    abstract：:The importance of Trp 800 in the calmodulin-binding site of myosin light chain kinase was investigated. Truncation mutants from Leu 447 to the C-terminus were expressed in E. coli and these were modified by point mutations of Trp 800 to Gly, Cys, Leu and Tyr. Trp at this position was more effective than any of the oth...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2076

    authors： Matsushima S,Huang YP,Dudas CV,Guerriero V Jr,Hartshorne DJ

    更新日期：1994-08-15 00:00:00

  • Efficient screening of ligand-receptor complex formation using fluorescence labeling and size-exclusion chromatography.

    abstract：:Evidence of a complex formation is a crucial step in the structural studies of ligand-receptor interactions. Here we presented a simple and fast approach for qualitative screening of the complex formation between the chimeric extracellular domain of the nicotinic acetylcholine receptor (α7-ECD) and three-finger protei...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.08.021

    authors： Kulbatskii DS,Shulepko MA,Sluchanko NN,Yablokov EO,Kamyshinsky RA,Chesnokov YM,Kirpichnikov MP,Lyukmanova EN

    更新日期：2020-10-29 00:00:00

  • Inhibition of Moloney murine leukemia virus reverse transcriptase by alpha-anomeric oligonucleotides.

    abstract：:After parallel hybridization to complementary template RNA, alpha-anomeric oligonucleotides are not primers for Moloney murine leukemia virus reverse transcriptase. As can be expected they are competitors of classical primer oligonucleotides (beta-anomeric). They therefore inhibit the RNA dependent DNA polymerase acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91367-3

    authors： Lavignon M,Bertrand JR,Rayner B,Imbach JL,Malvy C,Paoletti C

    更新日期：1989-06-30 00:00:00

  • Influence of cyclic nucleotides on the processing of the carbohydrate part of the alpha-subunit of human choriogonadotropin by first trimester human placenta tissue.

    abstract：:In pulse-chase experiments ([35S]Met as radioactive label) 4 intracellular forms of the alpha-subunit (apparent molecular weights of 11, 16.5, 19.5, and 23.4 kDa) were observed whereas almost no label was incorporated into the beta-subunit. The 23.4 kDa form was secreted as free alpha-subunit, the others were precurso...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92399-1

    authors： Hilf G,Merz WE

    更新日期：1989-02-28 00:00:00

  • Intracellular third loop-C-terminal tail interaction in prostaglandin EP3beta receptor.

    abstract：:The secondary structures of and the interactions between the intracellular domains (the three loops and the C-terminal tail) of the mouse-derived prostaglandin E2 receptor EP3beta subtype were investigated using peptides mimicking the domains. The N-termini of the peptides were palmitoylated to anchor on unilamellar v...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.180

    authors： Yano Y,Shimbo T,Sugimoto Y,Matsuzaki K

    更新日期：2008-07-11 00:00:00

  • Expression of epidermal growth factor receptor sequences as E. coli fusion proteins: applications in the study of tyrosine kinase function.

    abstract：:To investigate the functions of key domains of the epidermal growth factor receptor (EGFR), various EGFR-derived peptide sequences were expressed in Escherichia coli as glutathione S-transferase (GST) fusion proteins. The purified fusion proteins (GST-TK0-8) were tested as substrates for the tyrosine kinase activities...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91915-f

    authors： Koland JG,O'Brien KM,Cerione RA

    更新日期：1990-01-15 00:00:00

  • Binding site of the rat liver specific monoclonal antibody.

    abstract：:A rat liver-specific antigen (RLSA) lost its binding ability to the corresponding monoclonal antibody after treatment with N-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid. The specific antigen reacted with wheat germ agglutinin, l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80403-0

    authors： Sato K,Ikeda T,Osada Y

    更新日期：1988-05-16 00:00:00

  • Disulfide isoforms of recombinant glia maturation factor beta.

    abstract：:Recombinant human glia maturation factor beta (r-hGMF-beta) is a single-chain polypeptide (141 amino acid residues) containing three cysteines, at positions 7, 86 and 95. Nascent r-hGMF-beta exists in the reduced state and has no biological activity. The protein can be activated through oxidative refolding by incubati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91209-b

    authors： Zaheer A,Lim R

    更新日期：1990-09-14 00:00:00

  • The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum.

    abstract：:Ovarian progesterone secretion during the diestrus stage of the estrous cycle is produced by luteal cells derived from granulosa and thecal cells after the differentiation process that follows ovulation. Our results show that blockade of the preovulatory rise of ovarian ornithine decarboxylase (ODC), a key enzyme in p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00191-2

    authors： Bastida CM,Tejada F,Cremades A,Peñafiel R

    更新日期：2002-04-26 00:00:00

  • Protein-binding DNA regions inside and in the vicinity of the cytochrome P450bm-3 gene from Bacillus megaterium.

    abstract：:Protein-binding DNA-regions inside and in the vicinity of the phenobarbital-inducible P450bm-3 gene from Bacillus megaterium were characterized by gel-retardation technique and foot-printing analysis. Regions with induction-dependent protein binding capacity were shown to be situated in -279bp/-215bp, -215bp/+83bp and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1123

    authors： Gaidamakova EK,Karginov VA,Kovalenko SP

    更新日期：1994-02-15 00:00:00

  • Identification of 8-hydroxyguanine glycosylase activity in mammalian tissues using 8-hydroxyguanine specific monoclonal antibody.

    abstract：:Here we report the finding of glycosylase activity in mammalian tissues to remove 8-hydroxyguanine residues from DNA. To detect this activity, a synthetic duplex DNA containing an 8-hydroxyguanine residue was used as the substrate, and before the product was analyzed by HPLC-electrochemical detection (ECD), 8-hydroxyg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2427

    authors： Lee YS,Lee HS,Park MK,Hwang ES,Park EM,Kasai H,Chung MH

    更新日期：1993-11-15 00:00:00

  • Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development.

    abstract：:Cancer cell molecular mimicry of stem cells (SC) follows with enhanced proliferative and renewal capacities. In support, numerous mediators of SC self-renewal have been evinced to exhibit oncogenic potential. More and more researches showed that the embryonic stem cell self-renewal genes express in various cancer cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.016

    authors： Deng L,Xiang X,Yang F,Xiao D,Liu K,Chen Z,Zhang R,Feng G

    更新日期：2017-08-19 00:00:00

  • Haloperidol, a sigma receptor 1 antagonist, promotes ferroptosis in hepatocellular carcinoma cells.

    abstract：:Ferroptosis is a novel form of cell death, which is characterized by accumulation of reactive oxygen species (ROS). Sigma 1 receptor (S1R) has been suggested to function in oxidative stress metabolism. Both erastin and sorafenib significantly induced S1R protein expression. Haloperidol strongly promoted erastin- and s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.07.136

    authors： Bai T,Wang S,Zhao Y,Zhu R,Wang W,Sun Y

    更新日期：2017-09-30 00:00:00

  • Prevalence of polymorphic 21-hydroxylase gene (CA21HB) mutations in salt-losing congenital adrenal hyperplasia.

    abstract：:Using genomic restriction analysis of 14 unrelated patients with salt-losing congenital adrenal hyperplasia, we identified three different CA21HB mutation patterns: no detectable restriction fragment abnormalities (16/28 haplotypes), deletion of the active CA21HB gene (9/28), and apparent conversion of the active CA21...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91484-7

    authors： Jospe N,Donohoue PA,Van Dop C,McLean RH,Bias WB,Migeon CJ

    更新日期：1987-02-13 00:00:00

  • A novel method for viral display of ER membrane proteins on budded baculovirus.

    abstract：:The baculovirus expression system has been used to express large quantities of various proteins, including membrane receptors. Here, we reveal a novel property of this expression system to be that certain membrane proteins can be displayed on the budded virus itself. We introduced the genes encoding sterol regulatory ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01355-x

    authors： Urano Y,Yamaguchi M,Fukuda R,Masuda K,Takahashi K,Uchiyama Y,Iwanari H,Jiang SY,Naito M,Kodama T,Hamakubo T

    更新日期：2003-08-15 00:00:00

  • Nitration and chlorination of folic acid by peroxynitrite and hypochlorous acid, and the selective binding of 10-nitro-folate to folate receptor beta.

    abstract：:The aim of this work was to characterize folates modified by ONOO(-) and HOCl and to evaluate the binding capacity of folates modified by ONOO(-) to folate receptor alpha and beta. For the modification of folate by ONOO(-), folic acid was reacted with the combination of PMA activated PMN and PAPA NONOate or chemically...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02359-8

    authors： Nakamura M,Nagayoshi R,Ijiri K,Nakashima-Matsushita N,Takeuchi T,Matsuyama T

    更新日期：2002-10-11 00:00:00

  • Non-invasive imaging of the levels and effects of glutathione on the redox status of mouse brain using electron paramagnetic resonance imaging.

    abstract：:Glutathione (GSH) is the most abundant non-protein thiol that buffers reactive oxygen species in the brain. GSH does not reduce nitroxides directly, but in the presence of ascorbates, addition of GSH increases ascorbate-induced reduction of nitroxides. In this study, we used electron paramagnetic resonance (EPR) imagi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.02.134

    authors： Emoto MC,Matsuoka Y,Yamada KI,Sato-Akaba H,Fujii HG

    更新日期：2017-04-15 00:00:00

  • The membrane integration of a naturally occurring alpha-helical hairpin.

    abstract：:Helical hairpins, two closely spaced helical membrane spanning segments separated by a short surface turn, are thought to be common in integral membrane proteins. Here, we study the membrane integration of a naturally occurring helical hairpin from the secretory Na(+)-K(+)-2Cl(-) cotransporter NKCC1. This sequence is ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.02.149

    authors： Nagy A,Turner RJ

    更新日期：2007-05-04 00:00:00

  • Genomic structure and expression of the human fau gene: encoding the ribosomal protein S30 fused to a ubiquitin-like protein.

    abstract：:The fau gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV). This virus acquired the fau sequence in its reversed transcriptional orientation. Human and mouse fau cDNA's were identified and both encode a new protein of 133 AA. We show that fau (for FBR-MuSV asso...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91286-y

    authors： Kas K,Michiels L,Merregaert J

    更新日期：1992-09-16 00:00:00

  • Molecular cloning, gene localization, and structure of human cyclin B3.

    abstract：:Here we describe the molecular cloning of human cyclin B3, its localization, and its structure. It is localized in the subcentromeric region of the X chromosome, still not completely sequenced by the Human Genome Project. This cyclin B3 is unusually large for a mitotic cyclin. Its mRNAs were found in all tissues and w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6458

    authors： Lozano JC,Perret E,Schatt P,Arnould C,Peaucellier G,Picard A

    更新日期：2002-02-22 00:00:00

  • High glucose induced VEGF expression via PKC and ERK in glomerular podocytes.

    abstract：:Podocytes are the major site of vascular endothelial growth factor (VEGF) production in the kidney, and up-regulation of VEGF plays a critical role in the progression of diabetic nephropathy. Using a differentiated mouse podocyte cell line, we investigated the roles of protein kinase C (PKC) and extracellular signal-r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6138

    authors： Hoshi S,Nomoto K,Kuromitsu J,Tomari S,Nagata M

    更新日期：2002-01-11 00:00:00

  • COMP-Ang1 ameliorates leukocyte adhesion and reinforces endothelial tight junctions during endotoxemia.

    abstract：:Endotoxemia is characterized by multiple dysfunctions of the micro-vascular endothelium. Of these, vascular leakage is an initial event that aggravates vascular dysfunction can lead to systemic vascular collapse and organ failure. Thus, prevention of vascular leakage may ameliorate endotoxin-induced dysfunctions of bl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.02.096

    authors： Hwang JA,Lee EH,Lee SD,Park JB,Jeon BH,Cho CH

    更新日期：2009-04-17 00:00:00

  • Upstream stimulatory factors regulate the C/EBP alpha gene during differentiation of 3T3-L1 preadipocytes.

    abstract：:During adipocyte differentiation, CCAAT/enhancer-binding protein alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPalpha gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggestin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.01.008

    authors： Kim JW,Monila H,Pandey A,Lane MD

    更新日期：2007-03-09 00:00:00

  • Cleavage of recombinant enkephalin precursor by endoproteolytic activity in bovine chromaffin granules.

    abstract：:To identify endoproteolytic activity that processes the enkephalin precursor, a novel approach was undertaken for the production of model substrate in the form of recombinant 35S-(Met)-preproenkephalin (35S-(Met)-PPE), generated by in vitro transcription and translation of the rat PPE cDNA. Endoproteolytic activity in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92085-e

    authors： Hook VY,Hegerle D,Affolter HU

    更新日期：1990-03-16 00:00:00

  • Polygonatum cyrtonema lectin, a potential antineoplastic drug targeting programmed cell death pathways.

    abstract：:Polygonatum cyrtonema lectin (PCL), a mannose/sialic acid-binding plant lectin, has recently drawn a rising attention for cancer biologists because PCL bears remarkable anti-tumor activities and thus inducing programmed cell death (PCD) including apoptosis and autophagy in cancer cells. In this review, we focus on exp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2011.02.049

    authors： Wang SY,Yu QJ,Bao JK,Liu B

    更新日期：2011-03-25 00:00:00

  • Identification of two types of homologous DNA pairing activity in mouse cells.

    abstract：:We have identified two types of homologous DNA pairing activity in mouse cell extracts by a strand-transfer assay. Both activities are separated from each other by anion-exchange chromatography; neither of them needs ATP. One requires magnesium ion and is stimulated by Escherichia coli single-stranded DNA binding prot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91724-5

    authors： Mishina Y,Shimokawa T,Kawasaki K,Arai N,Shibata T,Koyama H

    更新日期：1992-06-30 00:00:00

  • Proprotein convertases regulate insulin-like growth factor 1-induced membrane-type 1 matrix metalloproteinase in VSMCs via endoproteolytic activation of the insulin-like growth factor-1 receptor.

    abstract：:The IGF-1 receptor (IGF-1R) and MT1-MMP are synthesized as larger precursor proproteins, which require endoproteolytic activation by the proprotein convertases (PCs) furin/PC5 to gain full biological activity. The aim of this study was to investigate the contribution of PCs to IGF-1R and/or MT1-MMP activation in vascu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.001

    authors： Stawowy P,Kallisch H,Kilimnik A,Margeta C,Seidah NG,Chrétien M,Fleck E,Graf K

    更新日期：2004-08-27 00:00:00

  • Superoxide-mediated release of iron from ferritin by some flavoenzymes.

    abstract：:NADH-lipoamide dehydrogenase mobilized iron from ferritin under aerobic conditions. Superoxide dismutase strongly inhibited this mobilization, indicating that the superoxide radical is generated by the enzymatic reaction and release iron from ferritin. Addition of lipoamide as an electron acceptor to NADH-lipoamide de...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92333-u

    authors： Bando Y,Aki K

    更新日期：1990-04-30 00:00:00

  • Photoaffinity labeling of gonadotropin releasing hormone receptors in control and desensitized pituitary cells.

    abstract：:Gonadotropin releasing hormone (GnRH), preincubated with cultured rat pituitary cells, induced down regulation of GnRH receptors in a time- and dose-dependent manner. The specific binding was inhibited by 50% after 30 min and maximal inhibition (70%) was obtained after 75 min preincubation with 1 microM GnRH. Preincub...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90572-2

    authors： Hazum E,Schvartz I

    更新日期：1984-12-14 00:00:00