Identification of 8-hydroxyguanine glycosylase activity in mammalian tissues using 8-hydroxyguanine specific monoclonal antibody.

Abstract:

:Here we report the finding of glycosylase activity in mammalian tissues to remove 8-hydroxyguanine residues from DNA. To detect this activity, a synthetic duplex DNA containing an 8-hydroxyguanine residue was used as the substrate, and before the product was analyzed by HPLC-electrochemical detection (ECD), 8-hydroxyguanine released by the enzymatic action was specifically collected from reactions using immunoaffinity columns of 8-hydroxyguanine specific monoclonal antibody. With the application of immunoaffinity column chromatography, a single peak of 8-hydroxyguanine free from many interfering peaks of unknown origin was clearly demonstrated in HPLC-ECD even with crude tissue extracts used as assay materials. All the tissues of the rats examined showed the activity, suggesting its presence is ubiquitous. The present coupled technique of immunoaffinity column chromatography and HPLC-ECD will be very useful to many future studies of 8-hydroxyguanine glycosylase such as its activity detection, characterization and purification, etc.

authors

Lee YS,Lee HS,Park MK,Hwang ES,Park EM,Kasai H,Chung MH

doi

10.1006/bbrc.1993.2427

subject

Has Abstract

pub_date

1993-11-15 00:00:00

pages

1545-51

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(83)72427-7

journal_volume

196

pub_type

杂志文章
  • Human CAAF1 gene--molecular cloning, gene structure, and chromosome mapping.

    abstract::We have isolated and characterized a cDNA and the gene of the human homologue of CAAF1, a novel member of the S100 calcium-binding protein family. The 276-bp open reading frame encoded a 92-amino acid polypeptide with a predicted molecular mass of 10,575 Da. The deduced amino acid sequence of human CAAF1 showed 66% ho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0600

    authors: Yamamura T,Hitomi J,Nagasaki K,Suzuki M,Takahashi E,Saito S,Tsukada T,Yamaguchi K

    更新日期:1996-04-16 00:00:00

  • Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum.

    abstract::Lipoteichoic acid is a major cell wall virulence factor of gram-positive bacteria. LTAs from various bacteria have differential immunostimulatory potentials due to heterogeneity in their structures. Although recent studies have demonstrated that LTA isolated from Lactobacillus plantarum (pLTA) has anti-inflammatory pr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.03.107

    authors: Jang KS,Baik JE,Han SH,Chung DK,Kim BG

    更新日期:2011-04-22 00:00:00

  • The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling.

    abstract::Myocyte enhancer factor 2C (MEF2C) plays an important role in cardiovascular development and is a key transcription factor for cardiac hypertrophy. Here, we describe MEF2C regulation by insulin-like growth factor-1 (IGF-1) and its role in IGF-1-induced cardiac hypertrophy. We found that IGF-1 addition to cultured rat ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.07.147

    authors: Muñoz JP,Collao A,Chiong M,Maldonado C,Adasme T,Carrasco L,Ocaranza P,Bravo R,Gonzalez L,Díaz-Araya G,Hidalgo C,Lavandero S

    更新日期:2009-10-09 00:00:00

  • Norepinephrine induces rapid and long-lasting phosphorylation and redistribution of connexin 43 in cortical astrocytes.

    abstract::Norepinephrine (NE) modulates brain functions depending on both the internal and external environment. While the neuromodulatory actions of NE have been well characterized, the response and involvement of cortical astrocytes to physiological noradrenergic systems remain largely unknown, especially at the molecular lev...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.09.021

    authors: Nuriya M,Morita A,Shinotsuka T,Yamada T,Yasui M

    更新日期:2018-10-12 00:00:00

  • The impact of CREB and its phosphorylation at Ser142 on inflammatory nociception.

    abstract::Peripheral noxious stimulation leads to phosphorylation and thereby activation of the transcription factor CREB in the spinal cord. CREB phosphorylation occurs mainly at serine 133, but the phosphorylation site at serine 142 may also be important. We investigated the impact of spinal CREB protein levels and phosphoryl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.07.148

    authors: Niederberger E,Ehnert C,Gao W,Coste O,Schmidtko A,Popp L,Gall CV,Korf HW,Tegeder I,Geisslinger G

    更新日期:2007-10-12 00:00:00

  • Loss of alpha I type I collagen gene expression in rat clonal bone cell lines is accompanied by DNA methylation.

    abstract::Four clonal cell lines subcloned from a clonal population of fetal rat calvaria cells show a loss of type I collagen synthesis. Northern blot analysis showed that the level of alpha 1(I) collagen mRNA expression in each of the clonal populations parallels the level of collagen protein expression in each of these cell ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)90836-x

    authors: Waye MM,Robinson R,Orfanides AG,Aubin JE

    更新日期:1989-08-15 00:00:00

  • Isolation of two biologically active peptides, erythrotropin I and erythrotropin II from fetal calf intestine.

    abstract::A bioassay based on the measurement of thymidine incorporation into trichloroacetic acid-insoluble materials in erythroid cell suspensions from fetal calf liver was used as the assay for purification of two small peptides (erythrotropins I and II) from fetal calf intestine. The peptides were purified using reversed-ph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(83)80169-7

    authors: Congote LF

    更新日期:1983-09-15 00:00:00

  • Binding and pharmacologic properties of peptides derived from human and rat angiotensin II (AII) mRNA.

    abstract::We investigated the binding and pharmacologic properties of peptides encoded by complementary mRNA derived from the human and rat angiotensinogen gene (human and rat IIA, respectively). Human IIA (identical with AII in 4 amino acids) inhibited binding of [125I]AII to rat adrenal glomerulosa particles (Ki = 0.62 +/- 0....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)92114-f

    authors: Wiest SA,Gesellchen PD,Zimmerman K,Steinberg MI

    更新日期:1990-07-31 00:00:00

  • Iron-molybdenum cofactor of nitrogenase: electrochemical determination of the electron stoichiometry of the oxidized/semi-reduced couple.

    abstract::The number of electrons involved in the more positive of the two redox couples of the iron-molybdenum cofactor of Azotobacter vinelandii nitrogenase has been investigated by controlled potential coulometry in both the oxidizing and reducing directions. A n value of 1 was determined for interconversion of the oxidized ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)80085-8

    authors: Schultz FA,Gheller SF,Newton WE

    更新日期:1988-04-29 00:00:00

  • A single nucleotide polymorphism of CYP2b6 found in Japanese enhances catalytic activity by autoactivation.

    abstract::A single nucleotide polymorphism (SNP) resulting in a substitution from Gln to His was found in exon 4 of the CYP2B6 gene in Japanese. The frequency of the variant allele was found to be 19.9%. The mutant- and the wild-type enzymes were expressed in Escherichia coli, and the effects of the single amino acid substituti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4524

    authors: Ariyoshi N,Miyazaki M,Toide K,Sawamura Yi,Kamataki T

    更新日期:2001-03-01 00:00:00

  • A CRE and the region occupied by a protein induced by growth factors contribute to up-regulation of cyclin D1 expression in hepatocytes.

    abstract::Induction of cyclin D1 expression is a critical feature of growth factor-induced cell proliferation in hepatocytes. To clarify the mechanisms regulating cyclin D1 gene expression, we isolated the rat cyclin D1 gene and analyzed the transcriptional regulatory elements in rat hepatoma cells and primary cultured rat hepa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)02867-x

    authors: Moriuchi A,Ido A,Nagata Y,Nagata K,Uto H,Hasuike S,Hori T,Hirono S,Hayashi K,Tsubouchi H

    更新日期:2003-01-10 00:00:00

  • The JNK1/JNK3 interactome--contributions by the JNK3 unique N-terminus and JNK common docking site residues.

    abstract::The c-Jun N-terminal Kinases (JNKs) play important roles in cell responses to stress or growth factor stimulation. The JNK1α1 isoform shares >90% identity with a predominantly neuronal JNK3α1 isoform, but JNK3α1 also includes a distinctive 38 amino acid N-terminal sequence. To address the outstanding question of the p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.09.122

    authors: Chen WK,Yeap YY,Bogoyevitch MA

    更新日期:2014-10-24 00:00:00

  • A novel method to visually determine the intracellular pH of xenografted tumor in vivo by utilizing fluorescent protein as an indicator.

    abstract::The alkalization of intracellular pH (pHin) advances together with enhancement of aerobic glycolysis within tumor cells (the Warburg effect), and that is responsible for the progression of tumor malignancy together with hypoxia and angiogenesis. But how they correlate each other during tumor growth is poorly understoo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.07.095

    authors: Tanaka S,Harada H,Hiraoka M

    更新日期:2015-09-04 00:00:00

  • Seleno compounds and glutathione peroxidase catalyzed decomposition of S-nitrosothiols.

    abstract::Seleno compounds such as selenocystamine and seleno-D, L-cystine were found to catalyze the decomposition of S-nitrosothiols (e.g. S-nitroso-glutathione and S-nitroso-N-acetyl-D, L-penicillamine) in the presence of different thiols (e.g. glutathione, N-acetyl-D-penicillamine and 2-mercaptoethanol), and liberate nitric...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1620

    authors: Hou Y,Guo Z,Li J,Wang PG

    更新日期:1996-11-01 00:00:00

  • Decreased contraction of glycated collagen lattices coincides with impaired matrix metalloproteinase production.

    abstract::Nonenzymatic glycation of extracellular matrix (ECM) proteins is increased in diabetes mellitus and aging and triggers cellular events leading to an imbalance in ECM homeostasis. We studied the influence of collagen glycation on matrix metalloproteinase production by dermal fibroblasts using the model of lattice cultu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1519

    authors: Rittié L,Berton A,Monboisse JC,Hornebeck W,Gillery P

    更新日期:1999-10-22 00:00:00

  • Functional characterization of lysophosphatidic acid receptor 1 mutants identified in rat cancer tissues.

    abstract::Lysophosphatidic acid (LPA), an extracellular lipid mediator, exerts various cellular effects through activation of LPA receptors, LPA1-LPA6, in many types of cells including cancer cells. We recently found several missense mutations of Lpar1 in rat cancer tissues. One of these mutations is located at the extracellula...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.03.118

    authors: Ishii S,Tsujiuchi T,Fukushima N

    更新日期:2017-05-06 00:00:00

  • Inhibition of ADP-ribosyltransferase activity of cholera toxin by MDL 12330A and chlorpromazine.

    abstract::ADP-ribosylation by cholera toxin of the guanine nucleotide binding regulatory protein (Gs) of rat liver membrane adenylate cyclase was inhibited by 0.1-1 mM MDL 12330A or 0.1-1 mM chlorpromazine. Basal as well as cholera toxin activated adenylate cyclase activity in liver membranes was also inhibited by the two drugs...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(84)91312-3

    authors: Bitonti AJ

    更新日期:1984-04-30 00:00:00

  • Stimulation of free radical generation in human leukocytes by various agents including tumor necrosis factor is a calmodulin dependent process.

    abstract::The mechanism(s) involved in the generation of free radicals in human leukocytes by phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMP), lipopolysaccharide (LPS), arachidonic acid (AA), and recombinant-tumor necrosis factor-1-alpha (r-TNF-1 alpha) was investigated. Calmodulin antagonists, chlo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)90626-x

    authors: Das UN,Padma M,Sagar PS,Ramesh G,Koratkar R

    更新日期:1990-03-30 00:00:00

  • Detection of antibody to hepatitis C E2/NS1 protein in patients with type C hepatitis.

    abstract::Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectabl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91595-h

    authors: Yokosuka O,Ito Y,Imazeki F,Ohto M,Omata M

    更新日期:1992-11-30 00:00:00

  • Granulins, a novel class of peptide from leukocytes.

    abstract::We report the isolation and characterization of a novel class of leukocyte peptides with possible cytokine-like activities which we call granulins. They are cystine-rich with molecular weights of approximately 6 Kda, except for granulin D, which appears to be a dimer. We present the sequence of one member of this fami...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)80908-8

    authors: Bateman A,Belcourt D,Bennett H,Lazure C,Solomon S

    更新日期:1990-12-31 00:00:00

  • The EGCg-induced redox-sensitive activation of endothelial nitric oxide synthase and relaxation are critically dependent on hydroxyl moieties.

    abstract::Several rich sources of polyphenols stimulate the endothelial formation of nitric oxide (NO), a potent vasoprotecting factor, via the redox-sensitive activation of the PI3-kinase/Akt pathway leading to the phosphorylation of endothelial NO synthase (eNOS). The present study examined the molecular mechanism underlying ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.01.112

    authors: Auger C,Kim JH,Chabert P,Chaabi M,Anselm E,Lanciaux X,Lobstein A,Schini-Kerth VB

    更新日期:2010-02-26 00:00:00

  • Mechanistic insights into human pre-mRNA splicing of human ultra-short introns: potential unusual mechanism identifies G-rich introns.

    abstract::It is unknown how very short introns (<65 nt; termed 'ultra-short' introns) could be spliced in a massive spliceosome (>2.7 MDa) without steric hindrance. By screening an annotated human transcriptome database (H-InvDB), we identified three model ultra-short introns: the 56-nt intron in the HNRNPH1 (hnRNP H1) gene, th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.05.112

    authors: Sasaki-Haraguchi N,Shimada MK,Taniguchi I,Ohno M,Mayeda A

    更新日期:2012-06-29 00:00:00

  • SNHG11 promotes cell proliferation in colorectal cancer by forming a positive regulatory loop with c-Myc.

    abstract::Dysregulation of long non-coding RNAs (lncRNA) have long been linked to the onset and development of colorectal cancer (CRC), yet the underlying mechanisms remain elusive. Small nucleolar RNA host gene 11 (SNHG11) is a novel lncRNA with few information about its role in development and progression of CRC. Here, we fou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.05.002

    authors: Huang W,Dong S,Cha Y,Yuan X

    更新日期:2020-07-05 00:00:00

  • Inhibition of interferon-gamma-activated nuclear factor-kappa B by cyclosporin A: A possible mechanism for synergistic induction of apoptosis by interferon-gamma and cyclosporin A in gastric carcinoma cells.

    abstract::We previously reported synergistic induction of apoptosis by IFN-gamma plus either cyclosporin A (CsA) or tacrolimus (FK506) in gastric carcinoma cells. In this study, we aimed to elucidate the mechanism for this synergistic induction of apoptosis. IFN-gamma plus CsA synergistically induced caspase-3 mediated apoptosi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)00853-2

    authors: Beppu K,Morisaki T,Matsunaga H,Uchiyama A,Ihara E,Hirano K,Kanaide H,Tanaka M,Katano M

    更新日期:2003-06-13 00:00:00

  • RhoC is essential for TGF-beta1-induced invasive capacity of rat ascites hepatoma cells.

    abstract::Transforming growth factor-beta1 (TGF-beta1) is a multifunctional growth factor that plays a role in cell proliferation, differentiation, extracellular matrix production, apoptosis, and cell motility. We show here that TGF-beta1 increased the invasiveness of MM1 cells, which are a highly invasive clone of rat ascites ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.05.068

    authors: Mukai M,Endo H,Iwasaki T,Tatsuta M,Togawa A,Nakamura H,Inoue M

    更新日期:2006-07-21 00:00:00

  • Dynamic expression pattern of Ca(2+)/calmodulin-dependent protein kinase II gene in the central nervous system of Drosophila throughout development.

    abstract::Calcium/calmodulin-dependent protein kinase II (CaM KII) is thought to be involved in the majority of the neuronal functions mediated by intracellular free Ca(2+), and has been implicated in long-term potentiation, learning, and memory. In this work, we have examined in detail the RNA expression pattern for the Drosop...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0868

    authors: Rachidi M,Lopes C,Takamatsu Y,Ohsako S,Benichou JC,Delabar JM

    更新日期:1999-07-14 00:00:00

  • Down-regulation of Homer1b/c attenuates group I metabotropic glutamate receptors dependent Ca²⁺ signaling through regulating endoplasmic reticulum Ca²⁺ release in PC12 cells.

    abstract::The molecular basis for group I metabotropic glutamate receptors (mGluR1 and 5) coupling to membrane ion channels and intracellular calcium pools is not fully understood. Homer is a family of post synaptic density proteins functionally and physically attached to target proteins at proline-rich sequences. In the presen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.07.044

    authors: Lv MM,Cheng YC,Xiao ZB,Sun MY,Ren PC,Sun XD

    更新日期:2014-08-08 00:00:00

  • Human VAPA and the yeast VAP Scs2p with an altered proline distribution can phenocopy amyotrophic lateral sclerosis-associated VAPB(P56S).

    abstract::A human isoform of the vesicle-associated membrane protein-associated proteins (VAPs), VAPB, causes amyotrophic lateral sclerosis eight due to the missense mutation of Pro-56, whereas human VAPA and the yeast VAP Scs2p proteins are not significantly affected by similar mutations. We have found that VAPA and Scs2p have...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.12.011

    authors: Nakamichi S,Yamanaka K,Suzuki M,Watanabe T,Kagiwada S

    更新日期:2011-01-14 00:00:00

  • Reduction of PTP1B by RNAi upregulates the activity of insulin controlled fatty acid synthase promoter.

    abstract::Metabolic deregulation accompanying type II diabetes is characterized by insulin resistance in peripheral tissues (liver, muscle, and adipose), mediated by impairments in insulin receptor (IR) signaling. Protein tyrosine phosphatase 1B (PTP1B) has been shown to be a negative regulator of IR autophosphorylation and thu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.02.016

    authors: Xu J,Li L,Qian Z,Hong J,Shen S,Huang W

    更新日期:2005-04-08 00:00:00

  • Identification and characterization of the zinc finger protein SjZF in Schistosoma japonicum.

    abstract::Schistosomiasis represents one of the most prevalent parasitic infections affecting over 249 million people worldwide. The pathological damage is mainly caused by the eggs laid by female schistosomes. Zinc finger proteins (ZFPs) usually play critical roles in many biological functions. In this study, we cloned, identi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.05.083

    authors: Zhang R,Li J,Xiang M,Hong Q,Xu B,Hu W

    更新日期:2018-07-02 00:00:00