Efficient screening of ligand-receptor complex formation using fluorescence labeling and size-exclusion chromatography.

abstract：:Vascular endothelial growth factor (VEGF) is a mitogen that is critically involved in vasculogenesis, angiogenesis, and hematopoiesis. However, what and how transcription factors participate in the regulation of vegf gene expression are not fully understood. Here we report the cloning and sequencing of the zebrafish v...

journal_title：Biochemical and biophysical research communications

authors： He C,Chen X

更新日期：2005-04-01 00:00:00

abstract：:We examined the effects of recombinant human C-reactive protein (rhCRP) on atherosclerosis-related factors in cultured human coronary artery endothelial and smooth muscle cells (HCAECs and HCASMCs). After removing endotoxin from commercial rhCRP preparations using the appropriate column, the purified (P)-rhCRP retaine...

journal_title：Biochemical and biophysical research communications

authors： Nagoshi Y,Kuwasako K,Cao YN,Kitamura K,Eto T

更新日期：2004-02-20 00:00:00

abstract：:Vomeronasal organs of female Wistar rats were exposed with sprayed urine preparations of male Wistar rats prior to sacrifice. Exposure to crude urine and ultrafiltrated urine preparation (<5000 Da) induced significant Fos expression, which is correlated with cellular activity, in the mitral/tufted cell layer of the ac...

journal_title：Biochemical and biophysical research communications

authors： Tsujikawa K,Kashiwayanagi M

更新日期：1999-06-24 00:00:00

abstract：:Inflammatory bowel disease (IBD) comprises two major subtypes, ulcerative colitis (UC) and Crohn's disease, which are multifactorial diseases that may develop due to genetic susceptibility, dysbiosis, or environmental factors. Environmental triggers of IBD include food-borne factors, and a previous nationwide survey i...

journal_title：Biochemical and biophysical research communications

authors： Tsuchiya M,Ito G,Hama M,Nagata S,Kawamoto A,Suzuki K,Shimizu H,Anzai S,Takahashi J,Kuno R,Takeoka S,Hiraguri Y,Sugihara HY,Mizutani T,Yui S,Oshima S,Tsuchiya K,Watanabe M,Okamoto R

更新日期：2021-01-20 00:00:00

abstract：:The large ribosomal subunit of the thermophilic fungus Thermomyces lanuginosus was reconstructed in three dimensions from electron micrographs. This is the first reported reconstruction of the eukaryotic complex and demonstrates specific structural features such as an intersubunit canyon and a potential channel for th...

journal_title：Biochemical and biophysical research communications

authors： Harauz G,Zuzan H,Kim PT,Holbrook JA

更新日期：1995-02-15 00:00:00

abstract：:Inhibitors of phenoloxidase were identified in pupae of the housefly, Musca domestica L. The phenoloxidase inhibitors were purified from final instar pupae of the housefly by a combination of ammonium sulfate fractionation, ion-exchange chromatography, gel filtration and reverse-phase high performance liquid chromatog...

journal_title：Biochemical and biophysical research communications

authors： Tsukamoto T,Ichimaru Y,Kanegae N,Watanabe K,Yamaura I,Katsura Y,Funatsu M

更新日期：1992-04-15 00:00:00

abstract：:Hypoxia inducible factor-1 (HIF-1) is a transcription factor composed of two subunits, HIF-1alpha and ARNT, which is activated under hypoxia. HIF-1alpha mRNA is expressed constitutively in a wide variety of cell types, whereas in some others HIF1A gene expression is upregulated by hypoxia. In this report, we show that...

journal_title：Biochemical and biophysical research communications

authors： Minet E,Ernest I,Michel G,Roland I,Remacle J,Raes M,Michiels C

更新日期：1999-08-02 00:00:00

abstract：:To identify novel psoriasis-associated genes, we focused on several ESTs (expressed sequence tags) whose expression was predominantly increased in the affected skin in patients with psoriasis vulgaris, as assessed by microarray assay. In this paper, a full-length cDNA corresponding to one of those ESTs (AI440266) was ...

journal_title：Biochemical and biophysical research communications

authors： Matsuzaka Y,Okamoto K,Tsuji H,Mabuchi T,Ozawa A,Tamiya G,Inoko H

更新日期：2002-10-11 00:00:00

abstract：:The alternatively spliced isoform of the nonmuscle myosin II heavy chain B (MHC-B) with an insert of 21 amino acids at the 50- to 20-kDa junction of the globular region of myosin has been demonstrated to be expressed specifically in the central nervous system (CNS) in chicken. To explore the role of this B2 inserted i...

journal_title：Biochemical and biophysical research communications

authors： Takahashi M,Hirano T,Uchida K,Yamagishi A

更新日期：1999-05-27 00:00:00

abstract：:Large tonsillar lymphocytes labeled with [3H]thymidine reverted to small lymphocytes with concomitant loss of [3H]DNA upon culturing. The decrease of labeled DNA content and size of large lymphocytes was demonstrated by flow cytometry and cell sorting. These observations suggest that stimulated lymphocytes may revert ...

journal_title：Biochemical and biophysical research communications

authors： Sasvari-Szekely M,Staub M,Taljanidisz J,Szabo G Jr,Takacs J,Antoni F

更新日期：1984-12-28 00:00:00

abstract：:The Ca(2+)-binding proteins regulate a number of cellular and extracellular activities and deregulations of S100 gene expression are associated with several human diseases. For example, S100A7 is upregulated in psoriatic skin, implicating a link with psoriasis, a chronic inflammatory dermatosis. We purified human S100...

journal_title：Biochemical and biophysical research communications

authors： Bürgisser DM,Siegenthaler G,Kuster T,Hellman U,Hunziker P,Birchler N,Heizmann CW

更新日期：1995-12-05 00:00:00

abstract：:Using affinity chromatography on immobilized transcortin of 125I-labeled, cholate-solubilized plasma membranes of human syncytiotrophoblast, a transcortin-binding protein with a minimal Mr of about 20 kDa has been isolated. It was found to be a sialoglycoprotein with an isoelectric point at pH 4.4 (about 5.0 after the...

journal_title：Biochemical and biophysical research communications

authors： Krupenko SA,Avvakumov GV,Strel'chyonok OA

更新日期：1991-06-14 00:00:00

abstract：:In this study, we describe the identification and cloning of a novel member of the nuclear receptor superfamily. This orphan receptor, referred to as ROR gamma, belongs to the ROR/RZR subfamily. The open reading frame of ROR gamma encodes a protein of 560 amino acid residues with a predicted molecular mass of 63 kD. T...

journal_title：Biochemical and biophysical research communications

authors： Hirose T,Smith RJ,Jetten AM

更新日期：1994-12-30 00:00:00

abstract：:Syntaxins are a family of membrane proteins believed to participate in docking/fusing of arriving vesicles during membrane sorting and secretion. Of the six mammalian syntaxins known, only brain syntaxin 1A/1B has been biochemically characterized in its endogenous form. Syntaxin 4 mRNA is expressed in selective tissue...

journal_title：Biochemical and biophysical research communications

authors： Sumitani S,Ramlal T,Liu Z,Klip A

更新日期：1995-08-15 00:00:00

abstract：:Rat type 1 angiotensin II receptor has two subtypes, namely type 1a and type 1b. The regulation of the expressions of these two subtype receptor mRNAs was studied by using a competitive polymerase chain reaction method. The expression of the type 1a mRNA in the liver was negatively and that of the type 1b mRNA in the ...

journal_title：Biochemical and biophysical research communications

authors： Iwai N,Inagami T,Ohmichi N,Nakamura Y,Saeki Y,Kinoshita M

更新日期：1992-10-15 00:00:00

abstract：:Lipopolysaccharide (LPS)-induced multiple organ injury was mediated in part by a transcription factor, nuclear factor-kappaB (NF-kappaB). Mice were pretreated with dexamethasone (DEX), an inhibitor of NF-kappaB activation, to elucidate its effects on LPS-induced early responses in vivo. Early responses measured 1 h af...

journal_title：Biochemical and biophysical research communications

authors： Aoki K,Ishida Y,Kikuta N,Kawai H,Kuroiwa M,Sato H

更新日期：2002-06-28 00:00:00

abstract：:A reverse micellar system containing Tween 85 and water in isopropylpalmitate was developed which permitted the solubilization of bacteria in the form of homogenous organic solutions. The presence of the bacteria in solution was demonstrated by light microscopy. Immediately after solubilization, isolated bacterial cel...

journal_title：Biochemical and biophysical research communications

authors： Häring G,Luisi PL,Meussdoerffer F

更新日期：1985-03-29 00:00:00

abstract：:We have established that differing effects of alpha1-adrenergic receptor (AR) subtypes on cell proliferation are due to differential coupling to the Gs/cAMP pathway; thus, both alpha1A- and alpha1B-ARs couple to Gs, while alpha1D-AR does not. To identify the region responsible for this difference in subtype-specific G...

journal_title：Biochemical and biophysical research communications

authors： Shinoura H,Shibata K,Hirasawa A,Tanoue A,Hashimoto K,Tsujimoto G

更新日期：2002-11-22 00:00:00

abstract：:We previously reported that novel targeted "hybrid peptide" in which epidermal growth factor receptor (EGFR) binding peptide was conjugated with lytic-type peptide had selective cytotoxic activity to EGFR expressing cancer cell lines, and in vivo analysis revealed that this EGFR-lytic peptide displayed significant ant...

journal_title：Biochemical and biophysical research communications

authors： Tada N,Horibe T,Haramoto M,Ohara K,Kohno M,Kawakami K

更新日期：2011-04-08 00:00:00

abstract：:Sugars will eventually be exported transporters (SWEETs) are a group of recently identified sugar transporters in plants that play important roles in diverse physiological processes. However, currently, limited information about this gene family is available in pineapple (Ananas comosus). The availability of the recen...

journal_title：Biochemical and biophysical research communications

authors： Guo C,Li H,Xia X,Liu X,Yang L

更新日期：2018-02-05 00:00:00

abstract：:It is commonly assumed that plants do not possess consciousness. Since the criterion for this assumption is usually human consciousness this assumption represents a top down attitude. It is obvious that plants are not animals and using animal criteria of consciousness will lead to its rejection in plants. However usin...

journal_title：Biochemical and biophysical research communications

authors： Calvo P,Baluška F,Trewavas A

更新日期：2020-10-17 00:00:00

abstract：:Adult stem cells provide a promising alternative for the treatment of decompensated liver cirrhosis. Our previous study showed that peripheral blood monocytes (PBMCs) from decompensated liver cirrhosis could differentiate into hepatocyte-like cells in vitro. We here aimed to investigate the differential potential of t...

journal_title：Biochemical and biophysical research communications

authors： Yan L,Han Y,Wang J,Liu J,He Y,Wang H,Fan D

更新日期：2008-07-11 00:00:00

abstract：:Stimulation of prostaglandin production in C-9 rat liver cells by transforming growth factor (TGF)-alpha was synergistic with that of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). TGF-alpha and TPA synergized the release of radiolabelled compounds from [3H]arachidonic acid prelabelled C-9 cells. 1-Ol...

journal_title：Biochemical and biophysical research communications

authors： Levine L,Xiao DM,Worth N,Lilley WE

更新日期：1985-07-16 00:00:00

abstract：:We have isolated calpromotin, a protein reported abundant in human red cells and shown to be of significance for KCl transport. We show that calpromotin is identical to a radical scavenger protein, thiol-specific antioxidant protein (TSA). Calpromotin is known to exist partially as a large complex of identical subunit...

journal_title：Biochemical and biophysical research communications

authors： Kristensen P,Rasmussen DE,Kristensen BI

更新日期：1999-08-19 00:00:00

abstract：:Akt/PKB plays a pivotal role in cell survival and proliferation. Previously, we reported that UV-irradiation induces extensive cell death in Akt2(-/-) mouse embryonic fibroblasts (MEFs) while Akt1(-/-) MEFs show cell cycle arrest. Here, we find that Akt1(-/-) MEFs exhibit phenotypic changes characteristics of senescen...

journal_title：Biochemical and biophysical research communications

authors： Jee HJ,Kim HJ,Kim AJ,Bae YS,Bae SS,Yun J

更新日期：2009-06-05 00:00:00

abstract：:Asialofetuin-tacked liposomes (AF-liposomes) encapsulating interferon (IFN)-gamma were bound and internalized into a human hepatoma cell line, HepG2 cells, selectively through asialoglycoprotein receptor, but not non-tacked liposomes (N-liposomes). AF-liposomal IFN-gamma was more effective for inhibition of viral DNA ...

journal_title：Biochemical and biophysical research communications

authors： Ishihara H,Hayashi Y,Hara T,Aramaki Y,Tsuchiya S,Koike K

更新日期：1991-01-31 00:00:00

abstract：:Acyl-coenzyme A:cholesterol acyltransferase (ACAT) catalyzes the synthesis of cholesteryl esters from cholesterol and long-chain fatty acids. The two ACAT enzymes, ACAT1 and ACAT2, lack sterol regulatory elements in their promoters and have not been thought to be transcriptionally regulated by cellular cholesterol. Ho...

journal_title：Biochemical and biophysical research communications

authors： Pramfalk C,Angelin B,Eriksson M,Parini P

更新日期：2007-12-14 00:00:00

abstract：:A mitosis inhibiting peptide pyroGlu-Glu-Asp-Ser-GlyOH has been isolated from mouse skin extracts. Both the biological and a synthetic peptide with the same structure reversibly inhibit epidermal mitoses in a curvilinear fashion after intraperitoneal injection. The two compounds are chromatographically identical. ...

journal_title：Biochemical and biophysical research communications

authors： Reichelt KL,Elgjo K,Edminson PD

更新日期：1987-08-14 00:00:00

abstract：:Inflammation has been established in recent years as a hallmark of cancer. Cancer Associated Fibroblasts (CAFs) support tumorigenesis by stimulating angiogenesis, cancer cell proliferation and invasion. We previously demonstrated that CAFs also mediate tumor-enhancing inflammation in a mouse model of skin carcinoma. B...

journal_title：Biochemical and biophysical research communications

authors： Erez N,Glanz S,Raz Y,Avivi C,Barshack I

更新日期：2013-08-02 00:00:00

abstract：:Mechanical loading of cultured mesangial cells has been shown to induce both increased production of matrix proteins and cell proliferation. This in vitro phenomenon has been implicated in the progression of glomerular sclerosis observed in a variety of glomerular diseases. However, it is not yet known how cells sense...

journal_title：Biochemical and biophysical research communications

authors： Hamasaki K,Mimura T,Furuya H,Morino N,Yamazaki T,Komuro I,Yazaki Y,Nojima Y

更新日期：1995-07-17 00:00:00