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Solubilization of bacterial cells in organic solvents via reverse micelles.

Abstract：

:A reverse micellar system containing Tween 85 and water in isopropylpalmitate was developed which permitted the solubilization of bacteria in the form of homogenous organic solutions. The presence of the bacteria in solution was demonstrated by light microscopy. Immediately after solubilization, isolated bacterial cells were observed, which by aging tend to form larger aggregates. Cells of Escherichia coli remained viable in this system for at least one day and retained beta-galactosidase activity for an even longer period as indicated by the hydrolysis of x-gal. Cells of an alkane-degrading strain of Acinetobacter calcoaceticus remained viable in the system for several days.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Häring G,Luisi PL,Meussdoerffer F

doi

10.1016/s0006-291x(85)80030-9

subject

Has Abstract

pub_date

1985-03-29 00:00:00

pages

911-5

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(85)80030-9

journal_volume

127

pub_type

杂志文章

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