Induction of platelet-derived growth factor chain A gene expression in human smooth muscle cells by oxidized low density lipoproteins.

abstract：:We have developed a novel vector system for the efficient assembly of polydactyl zinc fingers. Next to proteins that possess short canonical TGEKP linkers between all constituting zinc fingers we constructed proteins with longer, 12 amino acid linkers between two three-finger (3F) units and between three two-finger (2...

journal_title：Biochemical and biophysical research communications

authors： Neuteboom LW,Lindhout BI,Saman IL,Hooykaas PJ,van der Zaal BJ

更新日期：2006-01-06 00:00:00

abstract：:Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the cent...

journal_title：Biochemical and biophysical research communications

authors： Lee D,Hong J,Kim KJ

更新日期：2019-02-12 00:00:00

abstract：:Pathways formed of delocalized π-electron systems and polar groups of polypeptide chains bridged by hydrogen bonds are referred as π-H pathways. Suitable for electron transfer, these pathways in cytochrome c oxidase connect CuA, the source of electrons distributed in cytochrome c oxidase, with the metal centers, heme ...

journal_title：Biochemical and biophysical research communications

authors： Ramasarma T,Vaigundan D

更新日期：2019-03-05 00:00:00

abstract：:G13 belongs to the G12-subfamily of heterotrimeric regulatory G-proteins. Employing specific antibodies, we isolated G alpha 13 from bovine brain by a four-step purification protocol combining conventional and affinity chromatography. The use of ethylene glycol as a protective agent influenced the elution properties o...

journal_title：Biochemical and biophysical research communications

authors： Harhammer R,Nürnberg B,Spicher K,Schultz G

更新日期：1994-10-28 00:00:00

abstract：:Beta A3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the beta A3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that ...

journal_title：Biochemical and biophysical research communications

authors： McDermott JB,Cvekl A,Piatigorsky J

更新日期：1996-04-25 00:00:00

abstract：:Osteoblasts regulate bone formation and remodeling, and are main target cells of oxidative stress in the progression of osteonecrosis. The stem cell factor (SCF)-c-Kit pathway plays important roles in the proliferation, differentiation and survival in a range of cell types, but little is known about its functions in o...

journal_title：Biochemical and biophysical research communications

authors： Yang L,Wu Z,Yin G,Liu H,Guan X,Zhao X,Wang J,Zhu J

更新日期：2014-12-12 00:00:00

abstract：:Activation of muscarinic cholinergic receptors on 1321N1 human astrocytoma cells leads to Ca2+ mobilization as measured by quin 2 fluorescence. Acetylcholine and methacholine were full and potent agonists, while carbachol and muscarine, were fully efficacious but 6- and 10-fold less potent than acetylcholine. The carb...

journal_title：Biochemical and biophysical research communications

authors： Noronha-Blob L,Richard C,U'Prichard DC

更新日期：1987-08-31 00:00:00

abstract：:We have used HL-60 leukemia cells to investigate phosphatidylinositol 3-kinase (PI 3-K) during granulocytic differentiation at the nuclear level. Nuclei of HL-60 cells showed a constitutive presence of PI 3-K that increased when cells were treated with differentiating doses of ATRA. PI 3-K was also detected tightly bo...

journal_title：Biochemical and biophysical research communications

authors： Marchisio M,Bertagnolo V,Colamussi ML,Capitani S,Neri LM

更新日期：1998-12-18 00:00:00

abstract：:Endothelial cells participate in the inflammatory and immune reactions. Endothelial cell activation is a recurrent phenomenon linked to the pathogenesis of diverse human diseases, such as acute and chronic inflammation and cardiovascular disorders. Pro-inflammatory cytokines (e.g., IL-1, TNF) are well-known activators...

journal_title：Biochemical and biophysical research communications

authors： Takehana K,Konishi A,Oonuki A,Noguchi M,Fujita K,Iino Y,Kobayashi T

更新日期：2002-05-10 00:00:00

abstract：:The human promyelocytic leukemia cell line, HL-60, stimulated with PMA, produced human HGF-like immunoreactivity (HL-60 HGF), which was detected with human HGF-specific ELISA. The purified HL-60 HGF was indistinguishable from human HGF in the plasma of patients with fulminant hepatic failure by studies of subunit cons...

journal_title：Biochemical and biophysical research communications

authors： Nishino T,Kaise N,Sindo Y,Nishino N,Nishida T,Yasuda S,Masui Y

更新日期：1991-11-27 00:00:00

abstract：:The amino acid sequence of biliverdin-IX beta reductase (EC1.3.1.24) from human liver was determined by automated Edman degradation of peptides generated by enzymatic and chemical cleavages. The enzyme was a single polypeptide chain of 204 amino acid residues, and its amino acid sequence had no significant homology to...

journal_title：Biochemical and biophysical research communications

authors： Yamaguchi T,Komuro A,Nakano Y,Tomita M,Nakajima H

更新日期：1993-12-30 00:00:00

abstract：:ABCG2 (also called MXR (3), BCRP (4), or ABCP (5) is a recently-identified ABC half-transporter, which causes multidrug resistance in cancer. Here we report that the expression of the ABCG2 protein in Sf9 insect cells resulted in a high-capacity, vanadate-sensitive ATPase activity in isolated membrane preparations. AB...

journal_title：Biochemical and biophysical research communications

authors： Ozvegy C,Litman T,Szakács G,Nagy Z,Bates S,Váradi A,Sarkadi B

更新日期：2001-07-06 00:00:00

abstract：:The oxidative stress triggered by photodynamic therapy (PDT) involves generation of cytotoxic reactive oxygen species, including superoxide radical, accumulation of de novo-generated ceramide, and induction of apoptosis. Since PDT with the photosensitizer phthalocyanine Pc 4 induces mitochondrial damage and the supero...

journal_title：Biochemical and biophysical research communications

authors： Dolgachev V,Oberley LW,Huang TT,Kraniak JM,Tainsky MA,Hanada K,Separovic D

更新日期：2005-07-01 00:00:00

abstract：:K63-linked polyubiquitination of proteins regulates their trafficking into specific cellular pathways such as endocytosis and autophagy. CYLD, a deubiquitinase specific for K63-linked polyubiquitins, is present in high quantities at the postsynaptic density (PSD). It was previously shown that, under excitatory conditi...

journal_title：Biochemical and biophysical research communications

authors： Thein S,Pham A,Bayer KU,Tao-Cheng JH,Dosemeci A

更新日期：2014-07-18 00:00:00

abstract：:Major blood stage antimalarial drugs like chloroquine and artemisinin target the heme detoxification process of the malaria parasite. Hemozoin formation reactions in vitro using the Plasmodium falciparum histidine-rich protein-2 (Pfhrp-2), lipids, and auto-catalysis are slow and could not explain the speed of detoxifi...

journal_title：Biochemical and biophysical research communications

authors： Pandey AV,Babbarwal VK,Okoyeh JN,Joshi RM,Puri SK,Singh RL,Chauhan VS

更新日期：2003-09-05 00:00:00

abstract：:Oxygen evolution from Triton X-100 prepared photosystem II particles from spinach was inhibited 70-80% after treatment of the particles with high concentrations of salt (0.5 or 1 M NaCl). The salt-washed preparations were depleted of 16 and 24 kilodalton polypeptides. Nearly complete restoration of herbicide-sensitive...

journal_title：Biochemical and biophysical research communications

authors： Nakatani HY

更新日期：1984-04-16 00:00:00

abstract：:Recently, we reported that in various cell lines under conditions of deenergization of the mitochondrial membrane, the release of Ca(2+) from the endoplasmic reticulum (ER) does not produce the expected activation of store-operated calcium channels (SOCs) in the plasma membrane. In the present work, we examined the ac...

journal_title：Biochemical and biophysical research communications

authors： Wasniewska M,Karczmarewicz E,Pronicki M,Piekutowska-Abramczuk D,Zablocki K,Popowska E,Pronicka E,Duszyński J

更新日期：2001-05-11 00:00:00

abstract：:An insect antiviral compound was purified from Heliothis virescens larval hemolymph by gel-filtration high pressure liquid chromatography (HPLC) and C-18 reverse-phase HPLC and its structure was determined by mass spectrometry. The antiviral compound is an N-myristoylated-peptide containing six amino acids with calcul...

journal_title：Biochemical and biophysical research communications

authors： Ourth DD

更新日期：2004-07-16 00:00:00

abstract：:NOS activity has been recently described in airway epithelial cells. Because these cells are often ciliated we hypothesized that NO modulates airway ciliary beating. CBF was measured in cultured BBECs using video microscopy. L-NMMA, a NOS inhibitor, caused a 40% decrease in CBF following pre-stimulation with isoproter...

journal_title：Biochemical and biophysical research communications

authors： Jain B,Rubinstein I,Robbins RA,Leise KL,Sisson JH

更新日期：1993-02-26 00:00:00

abstract：:In RNA, function follows form. Mapping the surface of RNA molecules with chemical and enzymatic probes has revealed invaluable information about structure and folding. Hydroxyl radicals ((·)OH) map the surface of nucleic acids by cutting the backbone where it is accessible to solvent. Recent studies showed that a micr...

journal_title：Biochemical and biophysical research communications

authors： Schlatterer JC,Wieder MS,Jones CD,Pollack L,Brenowitz M

更新日期：2012-08-24 00:00:00

abstract：:SEZ-12 is one of the seizure-related cDNAs which was isolated by differential hybridization from primary cultured neurons from the mouse cerebral cortex with or without pentylenetetrazol (PTZ). SEZ-12 expression is transiently down-regulated in the mouse brain by injection of PTZ. To characterize SEZ-12, isolation of ...

journal_title：Biochemical and biophysical research communications

authors： Kajiwara K,Nagasawa H,Shimizu-Nishikawa K,Ookura T,Kimura M,Sugaya E

更新日期：1996-05-06 00:00:00

abstract：:Since otoacoustic emissions were discovered, the old idea of a second (active) filter in the cochlea has been pursued with considerable dedication. In this contribution a concept explaining the action of the second filter is presented, which is derived from high-frequency resonance: The outer hair cells are excited wh...

journal_title：Biochemical and biophysical research communications

authors： Fritze W

更新日期：1996-06-14 00:00:00

abstract：:Fas-associated protein with death domain (FADD) has been implicated in T lymphocytes, but the nature of FADD-dependent mechanism in early T cell development has not been completely elucidated. In this study, using T-cell specific deletion mice, we observed that FADD deficiency in thymocytes led to increased apoptosis ...

journal_title：Biochemical and biophysical research communications

authors： Zhang XY,Yang BY,Wang JY,Mo X,Zhang J,Hua ZC

更新日期：2014-08-22 00:00:00

abstract：:Tumor necrosis factor alpha (TNFalpha) is a pleiotropic cytokine involved in apoptotic cell death, cellular proliferation, differentiation, inflammation, and tumorigenesis. In tumors it is secreted by tumor associated macrophages and can have both pro- and anti-tumorigenic effects. To identify genes regulated by TNFal...

journal_title：Biochemical and biophysical research communications

authors： Guardiola-Serrano F,Haendeler J,Lukosz M,Sturm K,Melchner Hv,Altschmied J

更新日期：2008-11-28 00:00:00

abstract：:The peptidyl-prolyl isomerase Pin1 is prevalently overexpressed in human cancers and is regarded as a new diagnostic and therapeutic target. Pin1 interacts with several proteins involved in cell cycle events in a phosphorylation-dependent manner. Among them, NIMA (never in mitosis, gene A) was first identified to inte...

journal_title：Biochemical and biophysical research communications

authors： Chen J,Li L,Zhang Y,Yang H,Wei Y,Zhang L,Liu X,Yu L

更新日期：2006-03-24 00:00:00

abstract：:The effect of vitamin A supplementation on stearoyl-CoA desaturase gene 1 expression in mouse liver was characterized. Normal BALB/c mice were fed 0.01% and 0.1% retinol palmitate as components of nonpurified diets. This treatment resulted in a 3-fold and a 7-fold induction of SCD1 mRNA levels, respectively, as determ...

journal_title：Biochemical and biophysical research communications

authors： Miller CW,Waters KM,Ntambi JM

更新日期：1997-02-03 00:00:00

abstract：:Inflammation has been implicated in a variety of psychiatric disorders. We aimed to determine whether levels of complement C5 protein in the cerebrospinal fluid (CSF), which may reflect activation of the complement system in the brain, are altered in patients with major psychiatric disorders. Additionally, we examined...

journal_title：Biochemical and biophysical research communications

authors： Ishii T,Hattori K,Miyakawa T,Watanabe K,Hidese S,Sasayama D,Ota M,Teraishi T,Hori H,Yoshida S,Nunomura A,Nakagome K,Kunugi H

更新日期：2018-03-04 00:00:00

abstract：:The effect of phospholipids on IFN-gamma production in mouse T cells was investigated. Phosphatidylserine (PS), which has a negatively charged head group, completely inhibited IFN-gamma production in splenic naïve T cells and antigen-dependent IFN-gamma production in Th1 clone 42-6A cells, whereas other phospholipids,...

journal_title：Biochemical and biophysical research communications

authors： Yotsumoto S,Kakiuchi T,Aramaki Y

更新日期：2005-12-30 00:00:00

abstract：:Influenza A viruses are a major threat to human health and inflict a significant public health challenge worldwide. Hemagglutinin (HA) is the main contributor to the infectivity of the virus and is a potential target for the development of antiviral therapeutic agents. The stem region of HA, which has been shown to be...

journal_title：Biochemical and biophysical research communications

authors： Yan L,Wang H,Sun L,Liu Y,Sun J,Zhao X,Li Y,Xie X,Hu J

更新日期：2019-10-15 00:00:00

abstract：:Trypsin-like serine proteases are involved in diverse biological processes such as complement activation, tissue remodeling, cellular migration, tumor invasion, and metastasis. Here we report a novel human C1r-like serine protease analog, CLSPa, derived from dendritic cells (DC). The 487-residue CLSPa protein contains...

journal_title：Biochemical and biophysical research communications

authors： Lin N,Liu S,Li N,Wu P,An H,Yu Y,Wan T,Cao X

更新日期：2004-08-20 00:00:00