当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Rapid isolation of G alpha 13 from bovine brain membranes: supportive effect of ethylene glycol.

Rapid isolation of G alpha 13 from bovine brain membranes: supportive effect of ethylene glycol.

Abstract：

:G13 belongs to the G12-subfamily of heterotrimeric regulatory G-proteins. Employing specific antibodies, we isolated G alpha 13 from bovine brain by a four-step purification protocol combining conventional and affinity chromatography. The use of ethylene glycol as a protective agent influenced the elution properties of G alpha 13 markedly. Only in the presence of ethylene glycol (30% v/v) a clear separation of G alpha 13 from other G-proteins was achieved during the initial anion exchange chromatography. This allowed isolation of G alpha 13 by subunit exchange chromatography on beta gamma-agarose. G alpha 13 was only released from immobilized beta gamma-dimers via activation by AMF but not by GTP gamma S, pointing to a poor basal nucleotide exchange of this protein. In contrast, N-terminally truncated G alpha 13 did not bind to immobilized beta gamma-dimers.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Harhammer R,Nürnberg B,Spicher K,Schultz G

doi

10.1006/bbrc.1994.2535

subject

Has Abstract

pub_date

1994-10-28 00:00:00

pages

835-40

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72535-6

journal_volume

204

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Intradermal pregnenolone sulfate attenuates capsaicin-induced nociception in rats.

    abstract：:We have previously shown that the neurosteroid pregnenolone sulfate (PS) inhibits the capsaicin receptor-mediated current in rat dorsal root ganglion neurons. Here, we examined the effect of intradermal injection of PS into the rat hindpaw on capsaicin-induced nociception. Results revealed that PS co-injected with cap...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.08.076

    authors： Chen SC,Liu BC,Chen CW,Wu FS

    更新日期：2006-10-20 00:00:00

  • Phorbol 12-myristate 13-acetate activates rabbit neutrophils without an apparent rise in the level of intracellular free calcium.

    abstract：:The addition of low concentrations of phorbol 12-myristate 13-acetate to rabbit neutrophils induces cell aggregation, degranulation, increased oxygen consumption and an increase in the amount of actin associated with the cytoskeleton without a rise in the level of intracellular free calcium as measured using the fluor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90828-8

    authors： Sha'afi RI,White JR,Molski TF,Shefcyk J,Volpi M,Naccache PH,Feinstein MB

    更新日期：1983-07-29 00:00:00

  • Somatostatin inhibits interleukin 6 release from rat cortical type I astrocytes via the inhibition of adenylyl cyclase.

    abstract：:Interleukin 6 is a pleiotropic cytokine produced in the central nervous system (CNS) that has been involved in both direct neurotrophic activities and in the regulation of the production of acute phase proteins both at peripheral and central levels. In rat cortical type I astrocytes, interleukin 6 release is under the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6513

    authors： Grimaldi M,Florio T,Schettini G

    更新日期：1997-06-09 00:00:00

  • Phase separation in necrotic cells.

    abstract：:Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2. How can osmotic equilibriu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.08.123

    authors： Rana PS,Mudrak NJ,Lopez R,Lynn M,Kershner L,Model MA

    更新日期：2017-10-21 00:00:00

  • Hepatocellular metabolism of 4-hydroxy-2,3-nonenal is impaired in conditions of chronic cholestasis.

    abstract：:4-Hydroxy-2,3-nonenal is a major aldehydic end-product of lipid peroxidation known to exert several biological and cytotoxic effects and to be produced during conditions of chronic cholestasis. Here we report that viable hepatocytes isolated from cholestatic livers of bile duct-ligated rats (BDL hepatocytes) show a si...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2338

    authors： Leonarduzzi G,Parola M,Muzio G,Garramone A,Maggiora M,Robino G,Poli G,Dianzani MU,Canuto RA

    更新日期：1995-09-14 00:00:00

  • MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3'UTR.

    abstract：BACKGROUND AND AIM:Aberrant expression of miR-107 is involved in the development of several human cancers. However, the role of miR-107 in hepatocellular carcinoma (HCC) is not well documented. In the present study, we aim to explore the function of miR-107 in hepatocarcinogenesis. METHODS:Bioinformatics analysis was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.10.070

    authors： Wang Y,Chen F,Zhao M,Yang Z,Zhang S,Ye L,Gao H,Zhang X

    更新日期：2016-11-18 00:00:00

  • 5-Fluorocytosine combined with Fcy-hEGF fusion protein targets EGFR-expressing cancer cells.

    abstract：:Human epithelial cancers account for approximately 50% of all cancer deaths. This type of cancer is characterized by excessive activation and expression of the epidermal growth factor receptor (EGFR). The EGFR pathway is critical for cancer cell proliferation, survival, metastasis and angiogenesis. The EGF-EGFR signal...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.10.050

    authors： Lan KH,Shih YS,Chang CA,Yen SH,Lan KL

    更新日期：2012-11-16 00:00:00

  • Anti-ischemia/reperfusion of C1 inhibitor in myocardial cell injury via regulation of local myocardial C3 activity.

    abstract：:C3 is common to all pathways of complement activation augmenting ischemia/reperfusion (I/R)-induced myocardial injury and cardiac dysfunction. Complement inhibition with the complement regulatory protein, C1 inhibitor (C1INH), obviously exerts cardioprotective effects. Here, we examine whether C1INH regulates C3 activ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.09.023

    authors： Fu J,Lin G,Zeng B,Wu Z,Wu Y,Chu H,Qin G,Liang G,Li J,Gan X,Yu X,Li C,Liu D

    更新日期：2006-11-10 00:00:00

  • Molecular modeling of the human multidrug resistance protein 1 (MRP1/ABCC1).

    abstract：:Multidrug resistance protein 1 (MRP1/ABCC1) is a 190kDa member of the ATP-binding cassette (ABC) superfamily of transmembrane transporters that is clinically relevant for its ability to confer multidrug resistance by actively effluxing anticancer drugs. Knowledge of the atomic structure of MRP1 is needed to elucidate ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.141

    authors： DeGorter MK,Conseil G,Deeley RG,Campbell RL,Cole SP

    更新日期：2008-01-04 00:00:00

  • Human kallikrein 13 involvement in extracellular matrix degradation.

    abstract：:The human kallikrein family is a group of 15 serine protease genes clustered on chromosome 19q13.4 and shares a high degree of homology. These proteolytic enzymes have diverse physiological functions in many different tissues. Growing evidence suggests that many kallikreins are differentially expressed in cancer and m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.206

    authors： Kapadia C,Ghosh MC,Grass L,Diamandis EP

    更新日期：2004-10-22 00:00:00

  • Connexin 30.2 is expressed in mouse pancreatic beta cells.

    abstract：:Nowadays, connexin (Cx) 36 is considered the sole gap junction protein expressed in pancreatic beta cells. In the present research we investigated the expression of Cx30.2 mRNA and protein in mouse pancreatic islets. Cx30.2 mRNA and protein were identified in isolated islet preparations by qRT-PCR and Western blot, re...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.06.100

    authors： Coronel-Cruz C,Hernández-Tellez B,López-Vancell R,López-Vidal Y,Berumen J,Castell A,Pérez-Armendariz EM

    更新日期：2013-09-06 00:00:00

  • The enantioselective participation of (S)- and (R)-diaminovaleric acids in the formation of delta-aminolevulinic acid in cyanobacteria.

    abstract：:Although it is recognized that 4,5-diaminovaleric acid, formed from glutamate 1-semialdehyde, functions as the intermediate in the last step of delta-aminolevulinic acid formation from glutamate, the enantioselectivity of the participating glutamate 1-semialdehyde aminotransferase for 4,5-diaminovaleric acid has remai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80955-6

    authors： Friedmann HC,Duban ME,Valasinas A,Frydman B

    更新日期：1992-05-29 00:00:00

  • Short-hairpin RNA-mediated Heat shock protein 90 gene silencing inhibits human breast cancer cell growth in vitro and in vivo.

    abstract：:Hsp90 interacts with proteins that mediate signaling pathways involved in the regulation of essential processes such as proliferation, cell cycle control, angiogenesis and apoptosis. Hsp90 inhibition is therefore an attractive strategy for blocking abnormal pathways that are crucial for cancer cell growth. In the pres...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.04.032

    authors： Zuo K,Li D,Pulli B,Yu F,Cai H,Yuan X,Zhang X,Lv Z

    更新日期：2012-05-04 00:00:00

  • Silencing of Cav1.2 gene in neonatal cardiomyocytes by lentiviral delivered shRNA.

    abstract：:Cav1.2 (alpha1C) and Cav1.3 (alpha1D) L-type Ca channels are co-expressed in the heart. To date, there are no pharmacological or biophysical tools to separate alpha1D from alpha1C Ca currents (I(Ca-L)) in cardiomyocytes. Here, we established a physiological model to study alpha1D I(Ca-L) in native myocytes using RNA i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.150

    authors： Karnabi E,Qu Y,Mancarella S,Yue Y,Wadgaonkar R,Boutjdir M

    更新日期：2009-07-10 00:00:00

  • Many commonly used siRNAs risk off-target activity.

    abstract：:Using small interfering RNA (siRNA) to induce sequence specific gene silencing is fast becoming a standard tool in functional genomics. As siRNAs in some cases tolerate mismatches with the mRNA target, knockdown of genes other than the intended target could make results difficult to interpret. In an investigation of 3...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.175

    authors： Snøve O Jr,Holen T

    更新日期：2004-06-18 00:00:00

  • Growth and differentiation of rat hepatocytes: changes in transcription factors HNF-3, HNF-4, STAT-3, and STAT-5.

    abstract：:The liver enriched transcription factors HNF-3 and HNF-4 are known to play major roles in development and differentiation of hepatocytes. STAT-3 and STAT-5 are signaling peptides activated by a variety of cytokines and growth factors including HGF and EGF. Their role in hepatocyte growth and differentiation is yet to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9384

    authors： Runge D,Runge DM,Drenning SD,Bowen WC Jr,Grandis JR,Michalopoulos GK

    更新日期：1998-09-29 00:00:00

  • Acetylsalicylic acid antagonizes the bleeding-induced changes in hemoglobin proportions in normal adult rats.

    abstract：:Normal adult Sprague-Dawley rats were made anemic by repeated phlebotomy. Ion-exchange chromatography of anemic blood showed newborn like hemoglobin proportions, involving the same six hemoglobin components as is found when newborn and adult blood are compared. However, acetylsalicylic acid intake during anemia failed...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91176-9

    authors： Datta MC,Josephs J,Tolbert ME,Anderson J,Dowla H

    更新日期：1986-05-29 00:00:00

  • Elevated levels of beta defensin-1 mRNA in diabetic kidneys of GK rats.

    abstract：:We previously described the isolation of CDK4, representing the 3(')end of an unknown mRNA, using differential display [Biophys. Res. Commun. 232 (1997) 49]. To determine the identity of the CDK4 gene, full-length CDK4 cDNA clones were isolated from a diabetic kidney cDNA library. DNA sequencing revealed that CDK4 rep...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.034

    authors： Page RA,Malik AN

    更新日期：2003-10-17 00:00:00

  • Human and rat amylin have no effects on insulin secretion in isolated rat pancreatic islets.

    abstract：:Amylin, an islet amyloid peptide secreted by the pancreatic beta cell, has been proposed as a humoral regulator of islet insulin secretion. Four separate preparations of amylin were tested for effects on hormone secretion in both freshly isolated and cultured rat islets and in HIT-T15, hamster insulinoma cells. With a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90628-k

    authors： Broderick CL,Brooke GS,DiMarchi RD,Gold G

    更新日期：1991-06-28 00:00:00

  • TT virus is distributed in various leukocyte subpopulations at distinct levels, with the highest viral load in granulocytes.

    abstract：:When TT virus (TTV) DNA was quantitated in whole blood and plasma aliquots from 27 viremic individuals by real-time detection PCR that can detect essentially all TTV genotypes, the TTV load was 6.9 +/- 3.5 (mean +/- standard deviation)-fold higher in the whole blood than in the plasma samples [P < 0.002 (paired t test...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6183

    authors： Takahashi M,Asabe S,Gotanda Y,Kishimoto J,Tsuda F,Okamoto H

    更新日期：2002-01-11 00:00:00

  • Phosphorylation of voltage-dependent anion channel by c-Jun N-terminal Kinase-3 leads to closure of the channel.

    abstract：:Stress activated c-Jun N-terminal Kinase-3 (JNK3) has been reported to act on mitochondrion to promote neuronal cell death. Phosphorylation of mitochondrial Voltage-Dependent Anion Channel (VDAC) plays an important role in mitochondria-mediated cell death. Keeping these in view phosphorylation of rat brain VDAC by JNK...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.02.077

    authors： Gupta R,Ghosh S

    更新日期：2015-03-27 00:00:00

  • The establishment of a novel high-throughput screening system using RNA-guided genome editing to identify chemicals that suppress aldosterone synthase expression.

    abstract：:Aldosterone is synthesized in the adrenal by the aldosterone synthase CYP11B2. Although the control of CYP11B2 expression is important to maintain the mineral homeostasis, its overexpression induced by the depolarization-induced calcium (Ca2+) signaling activation has been reported to increase the synthesis of aldoste...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.11.020

    authors： Ito R,Morita M,Nakano T,Sato I,Yokoyama A,Sugawara A

    更新日期：2021-01-01 00:00:00

  • Structural characterization of the novel aminoglycoside phosphotransferase AphVIII from Streptomyces rimosus with enzymatic activity modulated by phosphorylation.

    abstract：:Aminoglycoside phosphotransferases represent a broad class of enzymes that promote bacterial resistance to aminoglycoside antibiotics via the phosphorylation of hydroxyl groups in the latter. Here we report the spatial structure of the 3'-aminoglycoside phosphotransferase of novel VIII class (AphVIII) solved by X-ray ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.06.097

    authors： Boyko KM,Gorbacheva MA,Korzhenevskiy DA,Alekseeva MG,Mavletova DA,Zakharevich NV,Elizarov SM,Rudakova NN,Danilenko VN,Popov VO

    更新日期：2016-09-02 00:00:00

  • In vivo assay of human NK-dependent ADCC using NOD/SCID/gammac(null) (NOG) mice.

    abstract：:Monoclonal antibodies are essential to the success of molecularly targeted therapies. Recently, numerous therapeutic antibodies have been developed for various diseases, including cancer and autoimmune diseases. Experimental systems to effectively evaluate these candidate antibodies are urgently needed. One of the mec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.07.145

    authors： Shiokawa M,Takahashi T,Murakami A,Kita S,Ito M,Sugamura K,Ishii N

    更新日期：2010-09-03 00:00:00

  • Tsukushi is required for anterior commissure formation in mouse brain.

    abstract：:The anterior commissure (AC) is one of the important commissure projections in the brain that conveys information from one side of the nervous system to the other. During development, the axons from the anterior AC (aAC) and the posterior AC (pAC) course in the same dorsoventral plane and converge into a common fascic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.127

    authors： Ito A,Shinmyo Y,Abe T,Oshima N,Tanaka H,Ohta K

    更新日期：2010-11-26 00:00:00

  • Insulin-degrading enzyme is capable of degrading receptor-bound insulin.

    abstract：:In the investigation of the intracellular sites of insulin degradation, it might be important whether receptor-bound insulin could be a substrate for insulin-degrading enzyme (IDE). Insulin receptor and IDE were purified from rat liver using a wheat germ agglutinin column and monoclonal anti-IDE antibody affinity colu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90436-6

    authors： Yonezawa K,Yokono K,Shii K,Hari J,Yaso S,Amano K,Sakamoto T,Kawase Y,Akiyama H,Nagata M

    更新日期：1988-01-29 00:00:00

  • The use of the novel substrate-heme complex approach in the derivation of a representation of the active site of the enzyme cholesterol side chain cleavage.

    abstract：:The previously reported substrate-heme complex approach is used to study the binding of type II inhibitors of the enzyme cholesterol side chain cleavage (CSCC), a cytochrome P-450 dependent enzyme involved in the oxidative cleaveage of the C(20)-C(22) bond of cholesterol. Using the derived model, we have rationalised ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3209

    authors： Ahmed S

    更新日期：2000-08-11 00:00:00

  • The role of apolipoprotein E in the elimination of liposomes from blood by hepatocytes in the mouse.

    abstract：:We evaluated the role of apolipoprotein E (apoE) in the clearance of neutral and negatively charged liposomes by hepatocytes in apoE-deficient mice. Negatively charged liposomes were cleared at identical rates in apoE-deficient and wild-type mice; neutral liposomes were cleared at a 3.6-fold slower rate in apoE-defici...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.137

    authors： Yan X,Kuipers F,Havekes LM,Havinga R,Dontje B,Poelstra K,Scherphof GL,Kamps JA

    更新日期：2005-03-04 00:00:00

  • Stabilization of C-terminal binding protein 2 by cellular inhibitor of apoptosis protein 1 via BIR domains without E3 ligase activity.

    abstract：:C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor that regulates many genes involved in normal cellular events. Because CtBP2 overexpression has been implicated in various human cancers, its protein levels must be precisely regulated. Previously, we reported that CtBP1 and CtBP1-mediated transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.098

    authors： Seo TW,Lee YT,Lee JS,Yoo SJ

    更新日期：2020-09-17 00:00:00

  • Pharmacological and signaling analysis of human chemokine receptor CCR-7 stably expressed in HEK-293 cells: high-affinity binding of recombinant ligands MIP-3beta and SLC stimulates multiple signaling cascades.

    abstract：:The chemokine receptor CCR-7 is expressed in T, NK, and dendritic cells in a time-ordered and stimulus-dependent manner. Thorough analyses of the pharmacological profiles of the recombinant ligands for CCR-7, MIP-3beta/ELC/CK-beta 11, and SLC/Exodus-2/TCA4/6C-kine, using CCR-7-expressing HEK-293E transfectants determi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1442

    authors： Sullivan SK,McGrath DA,Grigoriadis D,Bacon KB

    更新日期：1999-10-05 00:00:00