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Phase separation in necrotic cells.

Abstract：

:Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2. How can osmotic equilibrium be maintained between the bleb and CB? Our photobleaching and ultracentrifugation experiments indicate extensive protein aggregation. We hypothesize that protein aggregation within the CB shifts the chemical equilibrium and draws proteins out of the bleb; at the same time, aggregation reduces the effective molar concentration of protein in the CB, so that osmotic equilibrium between high-protein CB and low-protein necrotic blebs becomes possible.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Rana PS,Mudrak NJ,Lopez R,Lynn M,Kershner L,Model MA

doi

10.1016/j.bbrc.2017.08.123

subject

Has Abstract

pub_date

2017-10-21 00:00:00

pages

300-303

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)31706-0

journal_volume

492

pub_type

杂志文章

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