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3T3-L1 adipocytes express two isoforms of phospholipase D in distinct subcellular compartments.

Abstract：

:Phospholipase D has been implicated as an important enzyme in a range of cellular responses, including regulated secretion and the formation of secretory vesicles, cell proliferation and control of cell morphology. As insulin treatment of adipocytes has been shown to stimulate a phosphatidylcholine-specific phospholipase D and also modulates membrane trafficking, we wished to determine which isoform(s) of phospholipase D were present within adipocytes, to identify their subcellular distribution, and examine how this distribution may change in response to insulin. Using RT-PCR, 3T3-L1 adipocytes were found to express two isoforms of phospholipase D, specifically PLD1b and PLD2a. Using isoform-specific antibodies, PLD1 and PLD2 were found to be present predominantly in intracellular membranes, unlike the situation reported in other cells. Detailed analysis of the intracellular localisation of PLD1 and PLD2 revealed that these isoforms are differentially localised within adipocytes, implying functionally distinct roles for PLD activity in distinct subcellular compartments.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Millar CA,Jess TJ,Saqib KM,Wakelam MJ,Gould GW

doi

10.1006/bbrc.1998.0110

subject

Has Abstract

pub_date

1999-01-27 00:00:00

pages

734-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)90110-3

journal_volume

254

pub_type

杂志文章

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