A novel sgRNA selection system for CRISPR-Cas9 in mammalian cells.

abstract：:Treatment of CCl 39 cells with the impermeable iron II chelator bathophenanthroline disulfonate (BPS) inhibits both DNA synthesis and transplasma membrane electron transport. The inhibition persists when the BPS is removed, and the extract from 10(6) cells contains up to 1.28 nmoles iron II chelated to BPS. The BPS ir...

journal_title：Biochemical and biophysical research communications

authors： Alcain FJ,Löw H,Crane FL

更新日期：1994-08-30 00:00:00

abstract：:The rat hemopexin (Hx) gene was isolated and studies of its transcriptional regulation initiated. For analysis by a transient expression assay, the sequence between -2400 and +21 and sequential 5' truncates were linked to the chloramphenicol acetyltransferase (CAT) gene. HepG2 cells transfected with these CAT construc...

journal_title：Biochemical and biophysical research communications

authors： Nagae Y,Muller-Eberhard U

更新日期：1992-05-29 00:00:00

abstract：:Hypoxia inducible factor-1 (HIF-1) is a transcription factor composed of two subunits, HIF-1alpha and ARNT, which is activated under hypoxia. HIF-1alpha mRNA is expressed constitutively in a wide variety of cell types, whereas in some others HIF1A gene expression is upregulated by hypoxia. In this report, we show that...

journal_title：Biochemical and biophysical research communications

authors： Minet E,Ernest I,Michel G,Roland I,Remacle J,Raes M,Michiels C

更新日期：1999-08-02 00:00:00

abstract：:Oxysterol-binding protein-related proteins are implicated in the sensing and transporting lipids at the membrane contact sites. One of the members of the mammalian ORP family, ORP8, is thought to transport lipids through directly tethering both ER and PM membranes. Targeting to PM is thought to be mediated by N-termin...

journal_title：Biochemical and biophysical research communications

authors： Lee M,Fairn GD

更新日期：2018-02-19 00:00:00

abstract：:Cytochrome P450 3A4, the most abundant P450 form in human liver, exhibits a very broad substrate specificity and is of great importance for drug metabolism. The interindividual difference in the hepatic expression of CYP3A4 is considerable. In order to investigate possible genetic factor(s) causing this variation, the...

journal_title：Biochemical and biophysical research communications

authors： Westlind A,Löfberg L,Tindberg N,Andersson TB,Ingelman-Sundberg M

更新日期：1999-05-27 00:00:00

abstract：:Almost all bacteria require iron for growth and virulence expression. However, Listeria spp. do not produce any siderophore for iron acquisition. Representative strains of each of the six species of Listeria were examined for their ability to use various compounds as iron suppliers in iron-restricted medium. Here we s...

journal_title：Biochemical and biophysical research communications

authors： Coulanges V,Andre P,Vidon DJ

更新日期：1998-08-19 00:00:00

abstract：:A Golgi enriched fraction from rat brain was prepared. The preparation has no carboxypeptidase activity and is not contaminated with cytosol, mitochondria and lysosomes as judged by marker enzyme activities for these constituents. Associated with the Golgi membranes a putative opioid peptide processing activity was de...

journal_title：Biochemical and biophysical research communications

authors： Demmer W,Brand K

更新日期：1986-07-16 00:00:00

abstract：:Deltakephalin, Tyr-D-Thr-Gly-Phe-Leu-Thr (DTLET) was rationally designed as pure delta-probe from proposed models of mu and delta opiate receptors. On peripheral organs, deltakephalin displays a 3000 times higher inhibitory potency on the electrically stimulated mouse vas deferens (IC50 = 0.15 nM) as on the guinea pig...

journal_title：Biochemical and biophysical research communications

authors： Zajac JM,Gacel G,Petit F,Dodey P,Rossignol P,Roques BP

更新日期：1983-03-16 00:00:00

abstract：:Transplantation of normal myoblasts into dystrophic muscles is a potential treatment for Duchenne muscular dystrophy (DMD). However, the success of such grafts is limited by the immune system responses. To avoid rejection problems, autologous transplantation of the patient's corrected myoblasts has been proposed. Regr...

journal_title：Biochemical and biophysical research communications

authors： Huard C,Moisset PA,Dicaire A,Merly F,Tardif F,Asselin I,Tremblay JP

更新日期：1998-07-30 00:00:00

abstract：:Melatonin produced by the pineal gland suppresses inflammatory responses in innate immune cells. However, the mechanism of how melatonin affects inflammatory gene regulation remains unclear. Here we performed comprehensive microarray analysis combined with transcription factor binding site (TFBS) analysis using LPS-in...

journal_title：Biochemical and biophysical research communications

authors： Kadena M,Kumagai Y,Vandenbon A,Matsushima H,Fukamachi H,Maruta N,Kataoka H,Arimoto T,Morisaki H,Funatsu T,Kuwata H

更新日期：2017-04-01 00:00:00

abstract：:The correlation between the increase of [Ca2+]i and the activation of hydrolysis of phosphoinositide-4,5-bisphosphate and formation of inositol(1,4,5)trisphosphate in neutrophils treated with Fc receptor-binding agonists is still under discussion. In this communication evidence is presented supporting the conclusion t...

journal_title：Biochemical and biophysical research communications

authors： Della Bianca V,Grzeskowiak M,Dusi S,Rossi F

更新日期：1993-11-15 00:00:00

abstract：:A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line ...

journal_title：Biochemical and biophysical research communications

authors： Lahajnar G,Rupprecht A

更新日期：1986-11-26 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) plays a key role in exocrine secretion, including salivary glands. However, its functional expression in salivary glands has not been rigorously studied. In this study, we investigated the expression pattern and regulatory mechanism of CFTR in salivary glands ...

journal_title：Biochemical and biophysical research communications

authors： Shin YH,Lee SW,Kim M,Choi SY,Cong X,Yu GY,Park K

更新日期：2016-12-02 00:00:00

abstract：:AHNAK is a giant protein of approximately 700 kDa identified in human neuroblastomas and skin epithelial cells. Recently, we found that AHNAK knock-out (AHNAK(-/-)) mice have a strong resistance to high-fat diet-induced obesity. In this study, we applied (1)H NMR-based metabolomics with multivariate statistical analys...

journal_title：Biochemical and biophysical research communications

authors： Kim IY,Jung J,Jang M,Ahn YG,Shin JH,Choi JW,Sohn MR,Shin SM,Kang DG,Lee HS,Bae YS,Ryu DH,Seong JK,Hwang GS

更新日期：2010-12-17 00:00:00

abstract：:Although insulin-like growth factors (IGF) I and II bind with high affinity to structurally discrete receptors, they bind with a lesser affinity to each other's receptor. We have evaluated the affinity of five different IGF-I preparations (three natural IGF-I preparations, one synthetic preparation, and one recombinan...

journal_title：Biochemical and biophysical research communications

authors： Rosenfeld RG,Conover CA,Hodges D,Lee PD,Misra P,Hintz RL,Li CH

更新日期：1987-02-27 00:00:00

abstract：:Cav1.2 (alpha1C) and Cav1.3 (alpha1D) L-type Ca channels are co-expressed in the heart. To date, there are no pharmacological or biophysical tools to separate alpha1D from alpha1C Ca currents (I(Ca-L)) in cardiomyocytes. Here, we established a physiological model to study alpha1D I(Ca-L) in native myocytes using RNA i...

journal_title：Biochemical and biophysical research communications

authors： Karnabi E,Qu Y,Mancarella S,Yue Y,Wadgaonkar R,Boutjdir M

更新日期：2009-07-10 00:00:00

abstract：:MDP, a synthetic muramyl dipeptide, is capable of increasing the primary in vitro antibody response, to sheep erythrocytes, of purified B cells restored with a monokine and helper T cell factors, including Interleukin 2 and the late-acting T cell replacing factor (TRF). First, the possibility that the adjuvanticity of...

journal_title：Biochemical and biophysical research communications

authors： Souvannavong V,Adam A

更新日期：1984-11-30 00:00:00

abstract：:Different groups including ours have shown that curcumin induces melanoma cell apoptosis, here we focused the role of mammalian Sterile 20-like kinase 1 (MST1) in it. We observed that curcumin activated MST1-dependent apoptosis in cultured melanoma cells. MST1 silencing by RNA interference (RNAi) suppressed curcumin-i...

journal_title：Biochemical and biophysical research communications

authors： Yu T,Ji J,Guo YL

更新日期：2013-11-08 00:00:00

abstract：:Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as...

journal_title：Biochemical and biophysical research communications

authors： Van De Parre TJ,Martinet W,Schrijvers DM,Herman AG,De Meyer GR

更新日期：2005-02-04 00:00:00

abstract：:To tailor tissue-type plasminogen activator (tPA) to possess an affinity for the integrins, several mutants were constructed by introducing the Arg-Gly-Asp (RGD) sequene into the tPA molecule. These mutants were expressed in COS-1 cells and partially purified by lysine-Sepharose chromatography. The RGD-dependent bindi...

journal_title：Biochemical and biophysical research communications

authors： Yamada T,Shimada Y,Kikuchi M

更新日期：1996-11-12 00:00:00

abstract：:FRET has emerged as an important tool for studying intracellular processes and interactions between biomolecules. Intracellular donor and acceptor molecules are distributed in individual organelles that usually have complex non-continuous shape. Consequently, background pixels arising from fluorophore-free regions of ...

journal_title：Biochemical and biophysical research communications

authors： Stepensky D

更新日期：2007-08-03 00:00:00

abstract：:Extracellular vesicles (EVs) play an important role in biological functions and may feature innate therapeutic potential for diseases. In the present study, EVs released by osteoblasts at different stages of the mineralization process were investigated for their potential ability to promote bone formation. Results sho...

journal_title：Biochemical and biophysical research communications

authors： Wei Y,Tang C,Zhang J,Li Z,Zhang X,Miron RJ,Zhang Y

更新日期：2019-06-18 00:00:00

abstract：:Despite its utility, dipeptides have not been widely used due to the absence of an efficient manufacturing method. Recently, a novel method for effective production of dipeptides using l-amino acid alpha-ligase (Lal) is presented. Lal, which is only identified in Bacillus subtilis, catalyzes dipeptide synthesis from u...

journal_title：Biochemical and biophysical research communications

authors： Kino K,Nakazawa Y,Yagasaki M

更新日期：2008-07-04 00:00:00

abstract：:Brush border membrane vesicles (BBMVs) prepared from midguts of Choristoneura fumiferana larvae were pretreated separately with the three CryIA delta-endotoxins from Bacillus thuringiensis subsp. kurstaki HD-1. Crossed affinity immunoelectrophoresis of the solubilized toxin-BBMV complexes, using antibodies raised agai...

journal_title：Biochemical and biophysical research communications

authors： Pang AS

更新日期：1994-03-30 00:00:00

abstract：:The Sir proteins, namely Sir2, 3 and 4, have roles related to heterochromatin, but genome-wide studies have revealed their presence at many euchromatic loci, although the functional meaning of this is still not clear. Nhp6a is an abundant HMG-like protein in yeast, which has a role in transcription by modulating chrom...

journal_title：Biochemical and biophysical research communications

authors： Ciuffetta A,Salerno D,Camilloni G,Venditti S

更新日期：2015-05-22 00:00:00

abstract：:A 12kDa, heat stable protein (IF1) inhibiting hydrolytic activity of submitochondrial particles was purified to electrophoretic homogeneity from buffalo heart mitochondria. Specific activity of the purified fraction was > 5000 units/mg. Maximal inhibition was observed at pH 6.0 and was Mg++ and ATP dependent. Circular...

journal_title：Biochemical and biophysical research communications

authors： Sah JF,Kumar C,Mohanty P

更新日期：1993-08-16 00:00:00

abstract：:Cultured bovine pulmonary artery endothelial cells contain a second peptidyl dipeptidase, distinct from angiotensin-converting enzyme, present in an inactive form associated with a non-dialyzable inhibitor. Partial purification by glycine affinity chromatography separates enzyme from inhibitor to yield a preparation w...

journal_title：Biochemical and biophysical research communications

authors： Lanzillo JJ,Dasarathy Y,Stevens J,Fanburg BL

更新日期：1986-01-29 00:00:00

abstract：:A major component of amyloid deposits found in Alzheimer disease and Down syndrome is the beta/A4 peptide, which is derived from the Alzheimer amyloid protein precursor (APP). Recent evidence indicates that increases in APP expression and/or beta/A4 peptide accumulation may underlie the amyloidosis characteristic of t...

journal_title：Biochemical and biophysical research communications

authors： Buxbaum JD,Christensen JL,Ruefli AA,Greengard P,Loring JF

更新日期：1993-12-15 00:00:00

abstract：:SMCX/JARID1C was recently shown to be a histone H3 lysine 4 (H3K4) demethylase. Here, we have identified an SMCX isoform that predominantly resides in the cytoplasm, but still efficiently demethylates trimethylated H3K4. SMCX requires several functional domains for its demethylase activity and is also capable of formi...

journal_title：Biochemical and biophysical research communications

authors： Kim TD,Shin S,Janknecht R

更新日期：2008-02-08 00:00:00

abstract：:A procedure is described which employs pepstatin-agarose for the affinity purification of either HIV-1 or HIV-2 protease from two similar recombinant E. coli constructs that were developed for the expression of these enzymes. HIV-2 protease was routinely expressed at much higher levels than the HIV-1 enzyme and pepsta...

journal_title：Biochemical and biophysical research communications

authors： Rittenhouse J,Turon MC,Helfrich RJ,Albrecht KS,Weigl D,Simmer RL,Mordini F,Erickson J,Kohlbrenner WE

更新日期：1990-08-31 00:00:00