pH dependent conformational changes modulate functional activity of the mitochondrial ATPase inhibitor protein.

abstract：:Vitamin C, or ascorbic acid, decreases paracellular endothelial permeability in a process that requires rearrangement of the actin cytoskeleton. To define the proximal mechanism of this effect, we tested whether it might involve enhanced generation and/or sparing of nitric oxide (NO) by the vitamin. EA.hy926 endotheli...

journal_title：Biochemical and biophysical research communications

authors： May JM,Qu ZC

更新日期：2011-01-14 00:00:00

abstract：:The most widely used rate expression for single-substrate enzyme catalyzed reactions, namely the Michaelis-Menten kinetics is based upon the assumption that enzyme concentration is in excess of the substrate in the medium and the rate is mainly limited by the substrate concentration according to saturation kinetics. H...

journal_title：Biochemical and biophysical research communications

authors： Kargi F

更新日期：2009-04-24 00:00:00

abstract：:Gout patients receiving a combination of allopurinol and furosemide require higher allopurinol doses to achieve the target serum urate (SU) of <6 mg/dl (Stamp et al., 2012) [1]. Our study aimed to identify the molecular basis for this observation. We used a fluorimetric assay to determine the impact of furosemide and ...

journal_title：Biochemical and biophysical research communications

authors： Knake C,Stamp L,Bahn A

更新日期：2014-09-12 00:00:00

abstract：:We prepared a specific antibody against GIRK1, the major subunit of the G protein-gated K+ (KG) channel. Immunohistochemical study revealed that GIRK1 immunoreactivity was detected in the presynaptic, but not in the postsynaptic, region in the paraventricular nucleus of the rat hypothalamus (PVN). Therefore, activatio...

journal_title：Biochemical and biophysical research communications

authors： Morishige KI,Inanobe A,Takahashi N,Yoshimoto Y,Kurachi H,Miyake A,Tokunaga Y,Maeda T,Kurachi Y

更新日期：1996-03-18 00:00:00

abstract：:CHFR has been implicated as a tumor suppressor in a multitude of cancers. It was originally identified as a major component of the antephase checkpoint. Recently, CHFR was reported to interact with MAD2, an important component of the spindle assembly checkpoint, where CHFR knockdown resulted in mislocalization of MAD2...

journal_title：Biochemical and biophysical research communications

authors： Keller JA,Petty EM

更新日期：2011-06-10 00:00:00

abstract：:Abnormal perpetual inflammatory response and sequential cytokine-induced prostaglandin E2 (PGE2) play important roles in the pathogenesis of rheumatoid arthritis (RA). The underlying regulatory mechanism, however, remain largely unknown. Here, we discovered that expression level of Metastasis associated protein 1 (MTA...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Dong BW,Zheng ZH,Wu ZB,Li W,Ding J

更新日期：2016-04-29 00:00:00

abstract：:The oxidative stress triggered by photodynamic therapy (PDT) involves generation of cytotoxic reactive oxygen species, including superoxide radical, accumulation of de novo-generated ceramide, and induction of apoptosis. Since PDT with the photosensitizer phthalocyanine Pc 4 induces mitochondrial damage and the supero...

journal_title：Biochemical and biophysical research communications

authors： Dolgachev V,Oberley LW,Huang TT,Kraniak JM,Tainsky MA,Hanada K,Separovic D

更新日期：2005-07-01 00:00:00

abstract：:The oral mucosa is an active though poorly understood immunological site. Using an experimental animal system involving antigen priming into the oral mucosa of transgenic mice expressing T cell receptor (TCR) for a peptide antigen of hen-egg lysozyme (HEL), the expression of six chemokine receptors and seven chemokine...

journal_title：Biochemical and biophysical research communications

authors： Otten K,Dragoo J,Wang HC,Klein JR

更新日期：2003-04-25 00:00:00

abstract：:7,8-Dihydrobiopterin is not an intermediate in the de novo biosynthesis of tetrahydrobiopterin, the cofactor required for aromatic amino acid hydroxylations. However, N-acetyl-serotonin inhibition of sepiapterin reductase, an enzyme whose previously only known function was the reduction of sepiapterin to 7,8-dihydrobi...

journal_title：Biochemical and biophysical research communications

authors： Milstien S,Kaufman S

更新日期：1983-09-30 00:00:00

abstract：:Three immunoreactive prolactin proteins (24 Kd, 16 Kd, and 12 Kd) were identified in the rat brain using sodium dodecyl sulphate polyacrylamide gel electrophoresis, and western blot analyses. In male and female brains, the primary prolactin protein has a molecular weight of 24 Kd which is similar to that of pituitary ...

journal_title：Biochemical and biophysical research communications

authors： DeVito WJ

更新日期：1988-01-29 00:00:00

abstract：:Mutations in the human TCOF1 gene have been identified in patients with Treacher Collins Syndrome (Mandibulofacial Dysostosis), an autosomal dominant condition affecting the craniofacial region. We report the isolation of the entire mouse Tcof1 coding sequence (3960 bp) by performing a computer-based search for mouse ...

journal_title：Biochemical and biophysical research communications

authors： Paznekas WA,Zhang N,Gridley T,Jabs EW

更新日期：1997-09-08 00:00:00

abstract：:Major histocompatibility complex class II (MHC II) molecules are mainly expressed on antigen presentation cells and play an important role in immune response. It has been reported that MHC II molecules are also detected in serum as a soluble form (sMHC II molecules), and they are considered to be involved in the maint...

journal_title：Biochemical and biophysical research communications

authors： Shishido T,Kohyama M,Nakai W,Matsumoto M,Miyata H,Suenaga T,Arase H

更新日期：2019-01-29 00:00:00

abstract：:Gastrin-releasing peptide (GRP) is a neuropeptide that plays roles in various pathophysiological conditions including inflammatory diseases in peripheral tissues; however, little is known about whether GRP can directly regulate endothelial inflammatory processes. In this study, we showed that GRP promotes the adhesion...

journal_title：Biochemical and biophysical research communications

authors： Kim MK,Park HJ,Kim Y,Kim HJ,Bae SK,Bae MK

更新日期：2017-04-01 00:00:00

abstract：:Methotrexate (MTX) is a dihydrofolate reductase (DHFR) inhibitor widely used for treating human cancers, and overexpression of histone deacetylase (HDAC) is usually found in tumors. HDAC inhibitors (HDACi) can reactivate tumor suppressor genes and serve as potential anti-cancer drugs. In this study, we found that MTX ...

journal_title：Biochemical and biophysical research communications

authors： Yang PM,Lin JH,Huang WY,Lin YC,Yeh SH,Chen CC

更新日期：2010-01-15 00:00:00

abstract：:6-amino-4-(4-phenoxyphenylethylamino)quinazoline (QNZ) is an excellent quinazoline-containing NF-κB inhibitor also acting as a novel anticancer agent. Considering both the medicinal significance of quinazoline scaffold and the tunable functionality of Michael acceptor-centric pharmacophores in the electrophilicity-bas...

journal_title：Biochemical and biophysical research communications

authors： Shi H,Li Y,Ren X,Zhang Y,Yang Z,Qi C

更新日期：2017-04-29 00:00:00

abstract：:The physiologically active form of vitamin D, 1,25-dihydroxyvitamin D(3), plays an important role not only in the establishment and maintenance of calcium metabolism, but also in regulating cell growth and differentiation. Because the clinical usefulness of 1,25-dihydroxyvitamin D(3) is limited by its tendency to caus...

journal_title：Biochemical and biophysical research communications

authors： Gaschott T,Steinmeyer A,Steinhilber D,Stein J

更新日期：2002-01-11 00:00:00

abstract：:Several chemokines, belonging to both the CXC and CC classes, act as positive or negative regulators of angiogenesis. We sought to investigate the role of CXCL13, B cell-attracting chemokine 1 (BCA-1), also known as B-lymphocyte chemoattractant (BLC), on endothelial cell functions. We tested the effect of CXCL13 on HU...

journal_title：Biochemical and biophysical research communications

authors： Spinetti G,Camarda G,Bernardini G,Romano Di Peppe S,Capogrossi MC,Napolitano M

更新日期：2001-11-23 00:00:00

abstract：:Bryostatin-1 is a natural activator of protein kinase C and currently examined in phase I trials as anticancer agent. We found that Bryostatin-1 induced tumor necrosis factor alpha (TNF alpha) expression in the human cell line MONO-MAC-6. Using Northern blot analysis and a bioassay for the detection of the cytokine we...

journal_title：Biochemical and biophysical research communications

authors： Steube KG,Drexler HG

更新日期：1995-09-25 00:00:00

abstract：:To overcome the limitations of existing models, we developed a novel experimental in vivo platform for replacing mouse liver with functioning human liver tissue. To do this, a herpes simplex virus type 1 thymidine kinase (HSVtk) transgene was expressed within the liver of highly immunodeficient NOG mice (TK-NOG). Mous...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa M,Kawai K,Mitsui T,Taniguchi K,Monnai M,Wakui M,Ito M,Suematsu M,Peltz G,Nakamura M,Suemizu H

更新日期：2011-02-18 00:00:00

abstract：:[2-14C]-trans-2-hexadecenoyl CoA (16:1) and [2-14C]-trans-2-cis-8,11,14-eicosatetraenoyl CoA (20:4) were chemically synthesized and employed as competitive substrates for the liver microsomal trans-2-enoyl CoA reductase component of the fatty acid chain elongation system. Both 7.5 microM and 15 microM 20:4 competitive...

journal_title：Biochemical and biophysical research communications

authors： Nagi MN,Cook L,Prasad MR,Cinti DL

更新日期：1986-10-15 00:00:00

abstract：:Extracellular matrix facilitates anchorage-dependent cell survival via interaction of its arginine-glycine-aspartate (RGD) motif with integrins. In this report, we describe an unexpected, apoptosis-promoting the effect of immobilized RGD (iRGD) on tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis. Mesangial ce...

journal_title：Biochemical and biophysical research communications

authors： Moreno-Manzano V,Lucio-Cazana J,Konta T,Nakayama K,Kitamura M

更新日期：2000-10-22 00:00:00

abstract：:A body of indirect evidence has suggested the involvement of reactive oxygen species (ROS) in tumor promotion. However, direct evidence for the involvement of in situ generated ROS in tumor promotion is lacking at present. This study provides the first in situ evidence for the involvement of ROS in stage I tumor promo...

journal_title：Biochemical and biophysical research communications

authors： Giri U,Sharma SD,Abdulla M,Athar M

更新日期：1995-04-17 00:00:00

abstract：:In recent years a variety of recombinant methods have been developed for efficient production of bispecific antibodies (BsAb) in various formats. Bispecific diabody (bDAb), a 55-60 kDa molecule comprising two non-covalently associated cross-over single chain Fv (scFv) polypeptides, represents one of the most promising...

journal_title：Biochemical and biophysical research communications

authors： Lu D,Jimenez X,Witte L,Zhu Z

更新日期：2004-05-28 00:00:00

abstract：:The nucleotide sequence of human Go alpha was determined from a partial human brain cDNA clone and the sequence of the first two 5' coding exons of a human genomic Go alpha clone. Comparison of this sequence with bovine and rat Go alpha shows greater than 90% homology at the nucleotide and deduced amino acid level. Th...

journal_title：Biochemical and biophysical research communications

authors： Lavu S,Clark J,Swarup R,Matsushima K,Paturu K,Moss J,Kung HF

更新日期：1988-01-29 00:00:00

abstract：:Tripartite motif-containing protein (TRIM) family proteins are involved in a broad range of biological processes and, consistently, their alterations result in diverse pathological conditions such as genetic diseases, viral infection and cancer development. In this study, we found that one of the TRIM family proteins,...

journal_title：Biochemical and biophysical research communications

authors： Watanabe M,Tsukiyama T,Hatakeyama S

更新日期：2009-10-16 00:00:00

abstract：:The antiherpetic agent 9-beta-D-arabinofuranosyladenine (araA) needs to be phosphorylated to its 5'-triphosphate to be effective as an inhibitor of herpes simplex virus replication. Adenosine kinase and deoxycytidine kinase are assumed to convert araA to its 5'-monophosphate. We now found that araAMP is converted to i...

journal_title：Biochemical and biophysical research communications

authors： Balzarini J,De Clercq E

更新日期：1990-12-31 00:00:00

abstract：:In rat mesenteric vessels changes in external pH (pHo) alter tension. Changes in intracellular pH (pHi) and calcium ([Ca2+]i) have both been suggested to underlie the tension changes. As no simultaneous measurements of pHi and [Ca2+]i had been made, however, the relative importance and temporal relationship between pH...

journal_title：Biochemical and biophysical research communications

authors： Austin C,Dilly K,Eisner D,Wray S

更新日期：1996-05-15 00:00:00

abstract：:One of the major roles of brain-derived neurotrophic factor (BDNF) is to promote the differentiation and support the survival of neurons in the central nervous system. The objective of the present study was to evaluate the effect of BDNF on the fate of adult rat hippocampus-derived neural stem cells (AHPCs) transplant...

journal_title：Biochemical and biophysical research communications

authors： Suzuki T,Ooto S,Akagi T,Amemiya K,Igarashi R,Mizushima Y,Takahashi M

更新日期：2003-10-03 00:00:00

abstract：:We infected a mixed culture of primary rat astrocytes and microglia with a replication-defective adenovirus carrying the rat glia maturation factor (GMF) cDNA. Affymetrix microarray analysis showed a big increase in the expression of several major histocompatibility complex (MHC) class II proteins along with interleuk...

journal_title：Biochemical and biophysical research communications

authors： Zaheer A,Mathur SN,Lim R

更新日期：2002-06-07 00:00:00

abstract：:The human leukemia cell line KU812 had been described as an immature prebasophilic cell line and exhibits a potential to differentiate into mature basophils. We studied the effect of interleukin-4 (IL-4) on the basophilic differentiation of KU812 cells. When KU812 cells were cultured with 1 ng/ml IL-4, cellular histam...

journal_title：Biochemical and biophysical research communications

authors： Hara T,Yamada K,Tachibana H

更新日期：1998-06-29 00:00:00