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Generalized rate equation for single-substrate enzyme catalyzed reactions.

Abstract：

:The most widely used rate expression for single-substrate enzyme catalyzed reactions, namely the Michaelis-Menten kinetics is based upon the assumption that enzyme concentration is in excess of the substrate in the medium and the rate is mainly limited by the substrate concentration according to saturation kinetics. However, this is only a special case and the actual rate expression varies depending on the initial enzyme/substrate ratio (E(0)/S(0)). When the substrate concentration exceeds the enzyme concentration the limitation is due to low enzyme concentration and the rate increases with the enzyme concentration according to saturation kinetics. The maximum rate is obtained when the initial concentrations of the enzyme and the substrate are equal. A generalized rate equation was developed in this study and special cases were discussed for enzyme catalyzed reactions.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kargi F

doi

10.1016/j.bbrc.2009.02.155

subject

Has Abstract

pub_date

2009-04-24 00:00:00

pages

157-9

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)00444-6

journal_volume

382

pub_type

杂志文章

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