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Tat-mediated protein delivery in living Caenorhabditis elegans.

Abstract：

:The Tat protein from HIV-1 fused with heterologous proteins traverses biological membranes in a transcellular process called: protein transduction. This has already been successfully exploited in various biological models, but never in the nematode worm Caenorhabditis elegans. TAT-eGFP or GST-eGFP proteins were fed to C. elegans worms, which resulted in the specific localization of Tat-eGFP to epithelial intestinal cells. This system represents an efficient tool for transcellular transduction in C. elegans intestinal cells. Indeed, this approach avoids the use of tedious purification steps to purify the TAT fusion proteins and allows for rapid analyses of the transduced proteins. In addition, it may represent an efficient tool to functionally analyze the mechanisms of protein transduction as well as to complement RNAi/KO in the epithelial intestinal system. To sum up, the advantage of this technology is to combine the potential of bacterial expression system and the Tat-mediated transduction technique in living worm.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Delom F,Fessart D,Caruso ME,Chevet E

doi

10.1016/j.bbrc.2006.11.046

subject

Has Abstract

pub_date

2007-01-19 00:00:00

pages

587-91

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)02506-X

journal_volume

352

pub_type

杂志文章

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