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Nucleotides maintain the activity of Cav1.2 channels in guinea-pig ventricular myocytes.

Abstract：

:The activity of Cav1.2 Ca(2+) channels is maintained in the presence of calmodulin and ATP, even in cell-free patches, and thus a channel ATP-binding site has been suggested. In this study, we examined whether other nucleotides, such as GTP, UTP, CTP, ADP and AMP, could be substituted for ATP in guinea-pig ventricular myocytes. We found that all the nucleotides tested could re-prime the Ca(2+) channels in the presence of 1 μM calmodulin in the inside-out mode. The order of efficacy was ATP > GTP > UTP > ADP > CTP ≈ AMP. Thus, the presumed nucleotide-binding site in the channel seemed to favor a purine rather than pyrimidine base and a triphosphate rather than a di- or mono-phosphate group. Furthermore, a high concentration (10 mM) of GTP, UTP, CTP, ADP and AMP had inhibitory effects on the channel activity. These results provide information on the putative nucleotide-binding site(s) in Cav1.2 Ca(2+) channels.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Liu SY,Xu JJ,Minobe E,Gao QH,Feng R,Zhao MM,Guo F,Yang L,Hao LY,Kameyama M

doi

10.1016/j.bbrc.2015.03.111

subject

Has Abstract

pub_date

2015-05-08 00:00:00

pages

813-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)00577-X

journal_volume

460

pub_type

杂志文章

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