Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.

abstract：:Methanol dehydrogenase is a heterotetrameric enzyme containing the prosthetic group pyrroloquinoline quinone (PQQ), which catalyzes the oxidation of methanol to formaldehyde. The crystal structure of methanol dehydrogenase from Methylophilus W3A1, previously determined at high resolution, exhibits a non-planar configu...

journal_title：Biochemical and biophysical research communications

authors： Li J,Gan JH,Mathews FS,Xia ZX

更新日期：2011-03-25 00:00:00

abstract：:Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-l...

journal_title：Biochemical and biophysical research communications

authors： Gao C,Qi S,Liu K,Li D,Jin C,Duan S,Zhang M,Chen M

更新日期：2017-04-22 00:00:00

abstract：:Insect midgut peritrophic membrane (PM) is a functional structure that protects insects against chemical damage and microorganism infection. The essential component in PM is chitin and its synthesis is catalyzed by Class B chitin synthase (CHSB), which plays a unique role in chitin-containing organisms and thus repres...

journal_title：Biochemical and biophysical research communications

authors： Qu M,Liu T,Yang J,Yang Q

更新日期：2011-01-07 00:00:00

abstract：:We previously reported synergistic induction of apoptosis by IFN-gamma plus either cyclosporin A (CsA) or tacrolimus (FK506) in gastric carcinoma cells. In this study, we aimed to elucidate the mechanism for this synergistic induction of apoptosis. IFN-gamma plus CsA synergistically induced caspase-3 mediated apoptosi...

journal_title：Biochemical and biophysical research communications

authors： Beppu K,Morisaki T,Matsunaga H,Uchiyama A,Ihara E,Hirano K,Kanaide H,Tanaka M,Katano M

更新日期：2003-06-13 00:00:00

abstract：:We have used RT-PCR with degenerate transmembrane primers to clone members of the G-coupled protein receptor family from rat hypothalamic suprachiasmatic nuclei. We report here a novel clone, UHR-1, which encodes a candidate receptor that is most similar to the neuropeptide receptor family, including the tachykinins, ...

journal_title：Biochemical and biophysical research communications

authors： Welch SK,O'Hara BF,Kilduff TS,Heller HC

更新日期：1995-04-17 00:00:00

abstract：:Spiroplasma are wall-less bacteria which belong to the phylum Tenericutes that evolved from Firmicutes including Bacillus subtilis. Spiroplasma swim by a mechanism unrelated to widespread bacterial motilities, such as flagellar motility, and caused by helicity switching with kinks traveling along the helical cell body...

journal_title：Biochemical and biophysical research communications

authors： Takahashi D,Fujiwara I,Miyata M

更新日期：2020-12-17 00:00:00

abstract：:The purpose of this study was to identify mammalian cell line(s) which possess intrinsic enzymatic activity of beta-carotene 15, 15'-dioxygenase. This enzyme (EC1.13.11.21) converts beta-carotene to retinal (precursor of retinol and retinoic acid). To assess activity, cellular enzyme preparations were incubated with b...

journal_title：Biochemical and biophysical research communications

authors： During A,Albaugh G,Smith JC Jr

更新日期：1998-08-19 00:00:00

abstract：:The apicomplexan Toxoplasma gondii, an obligate intracellular parasite, can infect humans and a wide range of vertebrates. Following oral infection, the parasite invades tissues by crossing non-permissive biological barriers such as the placenta or the blood-brain barrier. But the molecular mechanisms underlying migra...

journal_title：Biochemical and biophysical research communications

authors： Buache E,Garnotel R,Aubert D,Gillery P,Villena I

更新日期：2007-07-27 00:00:00

abstract：:The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state lev...

journal_title：Biochemical and biophysical research communications

authors： Muto Y,Tohmatsu T,Yoshioka S,Nozawa Y

更新日期：1986-02-26 00:00:00

abstract：:Human prostasin was recently identified as a potential regulator of epithelial sodium channel (ENaC) function. Through the use of positional scanning combinatorial substrate libraries, prostasin was shown to have a preference for poly-basic substrates: in position P4 preference was for arginine or lysine; in P3 prefer...

journal_title：Biochemical and biophysical research communications

authors： Shipway A,Danahay H,Williams JA,Tully DC,Backes BJ,Harris JL

更新日期：2004-11-12 00:00:00

abstract：:Small-molecule inhibitors of tau fibrillization are under investigation as tools for interrogating the tau aggregation pathway and as potential therapeutic agents for Alzheimer's disease. Established inhibitors include thiacarbocyanine dyes, which can inhibit recombinant tau fibrillization in the presence of anionic s...

journal_title：Biochemical and biophysical research communications

authors： Honson NS,Jensen JR,Darby MV,Kuret J

更新日期：2007-11-09 00:00:00

abstract：:Treatment of the rat C6 glioma cell line with interleukin-1 beta (IL-1 beta) resulted in an accumulation of cytosolic phospholipase A2 (cPLA2) in mRNA level. The increase of cPLA2 in mRNA level was observed at 6 hours after treatment of IL-1 beta and reached to the maximal level at 12 hours. The accumulation also rema...

journal_title：Biochemical and biophysical research communications

authors： Ozaki M,Morii H,Qvist R,Watanabe Y

更新日期：1994-11-30 00:00:00

abstract：:Organoids mimicking the formation of the brain cortex have been demonstrated to be powerful tools for developmental studies as well as pathological investigations of brain malformations. Here, we report an integrated approach for the quantification of temporal neural production (neurogenic rate) in organoids derived f...

journal_title：Biochemical and biophysical research communications

authors： Kosodo Y,Suetsugu T,Kobayashi TJ,Matsuzaki F

更新日期：2017-01-29 00:00:00

abstract：:NADH-rubredoxin oxidoreductase (NROR), a flavoprotein from the obligately anaerobe Clostridium acetobutylicum is encoded by an ORF (nror) of 1140 nucleotides. Whereas primary structure analysis reveals that NROR has amino acid sequence patterns homologous with those involved in FAD and NAD-binding, the enzyme is dista...

journal_title：Biochemical and biophysical research communications

authors： Guedon E,Petitdemange H

更新日期：2001-07-13 00:00:00

abstract：:We previously reported transplantation of brain microvascular endothelial cells (MVECs) into cerebral white matter infarction model improved the animal's behavioral outcome by increasing the number of oligodendrocyte precursor cells (OPCs). We also revealed extracellular vesicles (EVs) derived from MVECs promoted surv...

journal_title：Biochemical and biophysical research communications

authors： Osawa S,Kurachi M,Yamamoto H,Yoshimoto Y,Ishizaki Y

更新日期：2017-06-17 00:00:00

abstract：:One point mutation which converts glycine-317 to aspartate of tissue-nonspecific alkaline phosphatase (TNSALP) was reported to be associated with lethal hypophosphatasia (Greenberg, C. R., et al. Genomics 17, 215-217, 1993). In order to define the molecular defect of TNSALP underlying the pathogenesis of hypophosphata...

journal_title：Biochemical and biophysical research communications

authors： Fukushi M,Amizuka N,Hoshi K,Ozawa H,Kumagai H,Omura S,Misumi Y,Ikehara Y,Oda K

更新日期：1998-05-29 00:00:00

abstract：:Normal human serum transferrin is present in several isoforms, due to differences in glycosylation. Transferrin has two potential glycosylation sites, both normally occupied by oligosaccharide chains. Two of the transferrin isoforms, called carbohydrate deficient transferrin, are specifically increased in patients wit...

journal_title：Biochemical and biophysical research communications

authors： Landberg E,Påhlsson P,Lundblad A,Arnetorp A,Jeppsson JO

更新日期：1995-05-16 00:00:00

abstract：:We investigated the genomic organization of pancreatic zymogen granule membrane-associated protein GP2, a GPI-anchored protein exhibiting self-aggregation at acidic pH, in order to construct a gene-knockout mouse. Cloning and analysis of lambda clones encoding GP2 from 129 Svj mouse genomic DNA libraries showed that t...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi K,Yanagihara K,Ishiguro K,Fukuoka S

更新日期：2004-09-17 00:00:00

abstract：:The Triton X-100 extract of the particulate fraction of porcine spleen contains a protein kinase which can be activated by phospholipid and the phorbol ester TPA but does not respond to phospholipid and calcium. The partially purified kinase has a molecular weight of 76 kDa (p76-kinase) and hence is somewhat smaller t...

journal_title：Biochemical and biophysical research communications

authors： Gschwendt M,Leibersperger H,Marks F

更新日期：1989-11-15 00:00:00

abstract：:Rat neuromedin U was isolated from the small intestine using mainly immunoaffinity chromatography and radioimmunoassay for pig neuromedin U-8. The amino acid sequence of rat neuromedin U was determined by microsequence analysis to be Tyr-Lys-Val-Asn-Glu-Tyr-Gln-Gly-Pro-Val-Ala-Pro-Ser-Gly-Gly- Phe-Phe-Leu-Phe-Arg-Pro-...

journal_title：Biochemical and biophysical research communications

authors： Minamino N,Kangawa K,Honzawa M,Matsuo H

更新日期：1988-10-14 00:00:00

abstract：:Prion diseases are fatal neurodegenerative diseases associated with structural conversion of α-helical prion protein (PrP) into its β-sheet rich isoform (PrPSc). Previous genetic analyses have indicated that several amino acid residues involved in the hydrophobic core of PrP (such as V180, F198, and V210) play a criti...

journal_title：Biochemical and biophysical research communications

authors： Kakuda K,Yamaguchi KI,Kuwata K,Honda R

更新日期：2018-11-17 00:00:00

abstract：:Sequence-specific hybridization of antisense and antigene agent to the target nucleic acid is an important therapeutic strategy to modulate gene expression. However, efficiency of such agents falls due to inherent intramolecular-secondary-structures present in the target that pose competition to intermolecular hybridi...

journal_title：Biochemical and biophysical research communications

authors： Kaur H,Wengel J,Maiti S

更新日期：2007-01-05 00:00:00

abstract：:Recombinant baculoviruses expressing full length and 3' truncated forms of c-DNA encoding the Drosophila melanogaster acetylcholinesterase (AChE) were constructed. Biochemical analyses showed that full length recombinant protein was enzymatically active and anchored to the cell membrane via a glycolipidic residue. DTT...

journal_title：Biochemical and biophysical research communications

authors： Chaabihi H,Fournier D,Fedon Y,Bossy JP,Ravallec M,Devauchelle G,Cérutti M

更新日期：1994-08-30 00:00:00

abstract：:Serum transferrin (tf), encoding an iron-binding glycoprotein, has been revealed to play important roles in iron transportation and immune response, and it also has been demonstrated to be valuable for phylogenetic analysis in vertebrates. However, the evolutionary conservation, expression profiles and positive select...

journal_title：Biochemical and biophysical research communications

authors： Liu XL,Wang YK,Ouyang S,Zhu YY,Li W,Hong XY,Xu HY,Zhu XP

更新日期：2018-12-02 00:00:00

abstract：:Traditional reconstitution of membrane cytochromes P450 monooxygenase system requires efficient solubilization of both P450 heme enzymes and redox partner NADPH dependent reductase, CPR, either in mixed micellar solution or by incorporation in liposomes. Here we describe a simple alternative approach to assembly of so...

journal_title：Biochemical and biophysical research communications

authors： Grinkova YV,Denisov IG,Sligar SG

更新日期：2010-07-23 00:00:00

abstract：:The multidrug resistance proteins P-glycoprotein (Pgp) and MRP1 are drug-efflux pumps. In this study, we compared the nucleotide triphosphatase activities of the isolated N-terminal nucleotide binding domains (NBD1) of Pgp and MRP1, and explored the potential role of the phosphorylation target domain of Pgp on the reg...

journal_title：Biochemical and biophysical research communications

authors： Wilkes DM,Wang C,Aristimuño PC,Castro AF,Altenberg GA

更新日期：2002-08-16 00:00:00

abstract：:Fecal supernatant or serum containing TT virus (TTV) of genotype 1a (10(5) copies/ml) from patients with acute TTV infection was inoculated intravenously into two naive chimpanzees. Serum samples were obtained weekly and tested for TTV DNA by genotype 1-specific polymerase chain reaction. TTV DNA was detected in chimp...

journal_title：Biochemical and biophysical research communications

authors： Tawara A,Akahane Y,Takahashi M,Nishizawa T,Ishikawa T,Okamoto H

更新日期：2000-11-19 00:00:00

abstract：:The concept of a glycolytic cancer cell was introduced by Warburg over 70 years ago. This perception has since become the rationale that drives a considerable proportion of basic research on cancer, and it influences the current strategies for the diagnosis, monitoring, and treatment of cancer. Here we review the data...

journal_title：Biochemical and biophysical research communications

authors： Zu XL,Guppy M

更新日期：2004-01-16 00:00:00

abstract：:Glial precursor cells (GPCs) are present in the adult human central nervous system (CNS) and they can be isolated and maintained in culture for in vitro studies. This study analysed expression of mGluR3 and mGluR5 metabotropic glutamate receptor (mGluR) mRNAs in GPCs. A2B5 surface antigen positive GPCs were isolated u...

journal_title：Biochemical and biophysical research communications

authors： Luyt K,Varadi A,Halfpenny CA,Scolding NJ,Molnar E

更新日期：2004-06-18 00:00:00

abstract：:S-nitrosothiols (SNOs) are endogenous signaling molecules with a broad spectrum of beneficial airway effects. SNOs are normally present in the airway, but levels tend to be low in cystic fibrosis (CF) patients. We and others have demonstrated that S-nitrosoglutathione (GSNO) increases the expression, maturation, and f...

journal_title：Biochemical and biophysical research communications

authors： Zaman K,Bennett D,Fraser-Butler M,Greenberg Z,Getsy P,Sattar A,Smith L,Corey D,Sun F,Hunt J,Lewis SJ,Gaston B

更新日期：2014-01-24 00:00:00