Abstract：
:Bacteriorhodopsin (BR) was reconstituted into artificial lipid membrane containing various charged lipid compositions. The proton pumping activity of BR under flash and continuous illumination, proton permeability across membrane, as well as the decay kinetics of the photocycle intermediate M(412) were studied. The results showed that lipid charges would significantly affect the orientation of BR inserted into lipid membranes. In liposomes containing anionic lipids, BRs were more likely to take natural orientation as in living cells. In neutral or positively charged liposomes, most BRs were reversely assembled, assuming an inside out orientation. Moreover, the lipids charges also affect BR's M intermediate kinetics, especially the slow component in M intermediate decay. The half-life M(412s) increased significantly in BRs in liposomes containing cationic lipids, while decreased in those in anionic liposomes.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Wang Z,Bai J,Xu Ydoi
10.1016/j.bbrc.2008.04.141subject
Has Abstractpub_date
2008-07-11 00:00:00pages
814-7issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(08)00855-3journal_volume
371pub_type
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journal_title：Biochemical and biophysical research communications
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