The mechanism of action of DuP 721, a new antibacterial agent: effects on macromolecular synthesis.

abstract：:We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by a single G----T substitution, affecting a 5'-TGTTCT-3' element that clos...

journal_title：Biochemical and biophysical research communications

authors： Claessens F,Celis L,Peeters B,Heyns W,Verhoeven G,Rombauts W

更新日期：1989-10-31 00:00:00

abstract：:It is commonly assumed that plants do not possess consciousness. Since the criterion for this assumption is usually human consciousness this assumption represents a top down attitude. It is obvious that plants are not animals and using animal criteria of consciousness will lead to its rejection in plants. However usin...

journal_title：Biochemical and biophysical research communications

authors： Calvo P,Baluška F,Trewavas A

更新日期：2020-10-17 00:00:00

abstract：:The membrane-bound acid alpha-glucosidase was purified partially (400-fold) from human placenta by solubilization with trypsin, concanavalin A-Sepharose chromatography, Ultrogel AcA-34 gel filtration, and Sephadex G-100 affinity chromatography. Two molecular forms of the enzyme were found in the final preparation of t...

journal_title：Biochemical and biophysical research communications

authors： Tsuji A,Suzuki Y

更新日期：1988-03-30 00:00:00

abstract：:Spontaneous Arg- mutants arose at high frequencies in Streptomyces lividans. Exposure to ethidium bromide increased the frequency of arg instability. In Pro+ strains the induced arg mutants were mainly argG, but in the proB mutants, a new mutation, argJ, prevailed which lacked ornithine acetyltransferase activity and ...

journal_title：Biochemical and biophysical research communications

authors： Chou CF,Chen CW

更新日期：1992-12-15 00:00:00

abstract：:Ganoderma lucidum (G. lucidum) is a popular medicinal mushroom that has been used as a home remedy for the general promotion of health and longevity in East Asia. The dried powder of G. lucidum, which was recommended as a cancer chemotherapy agent in traditional Chinese medicine, is currently popularly used worldwide ...

journal_title：Biochemical and biophysical research communications

authors： Stanley G,Harvey K,Slivova V,Jiang J,Sliva D

更新日期：2005-04-29 00:00:00

abstract：:An organic extract of Nitzschia alba cells possesses ionophoretic activity towards silicate, as it induces silicate transport across an organic phase or across synthetic lipid membranes. The activity is dependent upon Na+ and prefers silicon to germanium, a congener. The activity can be resolved into two apparently pu...

journal_title：Biochemical and biophysical research communications

authors： Bhattacharyya P,Volcani BE

更新日期：1983-07-18 00:00:00

abstract：:The need to develop anti-influenza drugs with novel antiviral mechanisms is urgent because of the rapid rate of antigenic mutation and the emergence of drug-resistant viruses. We identified a novel anti-influenza molecule by screening 861 plant-derived natural components using a high-throughput image-based assay that ...

journal_title：Biochemical and biophysical research communications

authors： Chang SY,Park JH,Kim YH,Kang JS,Min JY

更新日期：2016-03-04 00:00:00

abstract：:Macroautophagy is a conserved degradative pathway and its deterioration is linked to disturbances in cellular proteostasis and multiple diseases. Here, we show that the RAB GTPase RAB18 modulates autophagy in primary human fibroblasts. The knockdown of RAB18 results in a decreased autophagic activity, while its overex...

journal_title：Biochemical and biophysical research communications

authors： Feldmann A,Bekbulat F,Huesmann H,Ulbrich S,Tatzelt J,Behl C,Kern A

更新日期：2017-05-06 00:00:00

abstract：:An early signaling event during the adhesion and spreading of cells is integrin-mediated tyrosine phosphorylation of the cytoskeletal adaptor protein paxillin and the non-receptor tyrosine kinase pp125(FAK) at focal contacts. To determine the influence of surface-charge and -adsorbed adhesion proteins on this signalin...

journal_title：Biochemical and biophysical research communications

authors： Sommerfeldt DW,McLeod KJ,Rubin CT,Hadjiargyrou M

更新日期：2001-07-13 00:00:00

abstract：:Two species of scarab beetles, the Osaka beetle (Anomala osakana) and the Japanese beetle (Popillia japonica), utilize the opposite enantiomers of japonilure, (Z)-5-(1-decenyl)oxacyclopentan-2-one, as their sex pheromones. Each species produces only one of the enantiomers that functions as its own sex pheromone and as...

journal_title：Biochemical and biophysical research communications

authors： Wojtasek H,Hansson BS,Leal WS

更新日期：1998-09-18 00:00:00

abstract：:G13 belongs to the G12-subfamily of heterotrimeric regulatory G-proteins. Employing specific antibodies, we isolated G alpha 13 from bovine brain by a four-step purification protocol combining conventional and affinity chromatography. The use of ethylene glycol as a protective agent influenced the elution properties o...

journal_title：Biochemical and biophysical research communications

authors： Harhammer R,Nürnberg B,Spicher K,Schultz G

更新日期：1994-10-28 00:00:00

abstract：:The oxygen reaction of Nitrosomonas europaea cytochrome c oxidase containing either 2Cu or 1Cu per two heme a molecules was investigated by the flow-flash technique at 20 degrees C. The reaction profiles of the bacterial enzyme were essentially the same as those of bovine heart cytochrome c oxidase, although the rate ...

journal_title：Biochemical and biophysical research communications

authors： Orii Y,Sakamoto T,Fukumori Y,Yamanaka T

更新日期：1992-02-14 00:00:00

abstract：:We have compared the coupling of human alpha(1A)-, alpha(1B)-, and alpha(1D)-adrenoceptors (expressed at approximately 2000 fmol/mg protein in Chinese hamster ovary cells) to cellular growth promotion (as assessed by [(3)H]thymidine incorporation) and related signaling mechanisms. Maximum elevation of intracellular Ca...

journal_title：Biochemical and biophysical research communications

authors： Keffel S,Alexandrov A,Goepel M,Michel MC

更新日期：2000-06-16 00:00:00

abstract：:Myxothiazol is a respiratory chain complex III (CIII) inhibitor that binds to the ubiquinol oxidation site Qo of CIII. It blocks electron transfer from ubiquinol to cytochrome b and thus inhibits CIII activity. It has been utilized as a tool in studies of respiratory chain function in in vitro and cell culture models....

journal_title：Biochemical and biophysical research communications

authors： Davoudi M,Kallijärvi J,Marjavaara S,Kotarsky H,Hansson E,Levéen P,Fellman V

更新日期：2014-04-18 00:00:00

abstract：:Mammalian metallothioneins (MTs) possess eight highly conserved lysine residues, including three in each of two metal binding domains. We used site-directed mutagenesis to replace these intradomain lysines in Chinese hamster ovary MT2 with glutamic acid and/or glutamine. These mutant MTs were expressed in a metal sens...

journal_title：Biochemical and biophysical research communications

authors： Cody CW,Huang PC

更新日期：1994-07-29 00:00:00

abstract：:Porphyromonas gingivalis acquires heme through an outer-membrane heme transporter HmuR and heme-binding hemophore-like lipoprotein HmuY. Here, we compare binding of iron(III) mesoporphyrin IX (mesoheme) and iron(III) deuteroporphyrin IX (deuteroheme) to HmuY with that of iron(III) protoporphyrin IX (protoheme) and pro...

journal_title：Biochemical and biophysical research communications

authors： Wojaczyński J,Wójtowicz H,Bielecki M,Olczak M,Smalley JW,Latos-Grażyński L,Olczak T

更新日期：2011-07-29 00:00:00

abstract：:Standardized RT-PCR (StaRT-PCR) enables numerical quantification as well as intra- and inter-laboratory comparison of gene expression. Multiplex StaRT-PCR, using two rounds of amplification, was conducted on Stratagene Universal Reference RNA. In the first round, cDNA, competitive template (CT) mix, and primers for up...

journal_title：Biochemical and biophysical research communications

authors： Crawford EL,Warner KA,Khuder SA,Zahorchak RJ,Willey JC

更新日期：2002-04-26 00:00:00

abstract：:Stellate cell profibrotic gene induction and transdifferentiation are central events in liver fibrosis. Oxidative stress has been implicated as an activator of the transcription factors Nrf2 and AP-1 through shared kinase signaling pathways that also purportedly contribute to stellate cell activation. The present stud...

journal_title：Biochemical and biophysical research communications

authors： Reichard JF,Petersen DR

更新日期：2004-09-24 00:00:00

abstract：:Amantadine and oseltamivir are used to treat influenza A virus infections; however, resistance to these drugs has been widely reported throughout the world. In this study, the frequency and genetic characteristics of the drug-resistant influenza A viruses that circulated in Thailand from 2006 to 2008 were investigated...

journal_title：Biochemical and biophysical research communications

authors： Bai GR,Chittaganpitch M,Kanai Y,Li YG,Auwanit W,Ikuta K,Sawanpanyalert P

更新日期：2009-12-18 00:00:00

abstract：:Adhesive ascidians have caused serious biofouling problems and huge economic losses in marine ecosystems. However, adhesion mechanisms, particularly on functional proteins involved in ascidian adhesion, remain largely unexplored. Here, we identified 26 representative stolon proteins from the highly invasive fouling as...

journal_title：Biochemical and biophysical research communications

authors： Li S,Huang X,Chen Y,Li X,Zhan A

更新日期：2019-02-26 00:00:00

abstract：:The pathway employed for pentachlorophenol (PCP) degradation by an aerobic, chlorophenol-utilizing Flavobacterium sp. was initiated by conversion of PCP to tetrachloro-p-hydroquinone (TCH). 18O labelling experiments demonstrated that the first dechlorination, where a hydroxyl replaced the chlorine at PCP ring position...

journal_title：Biochemical and biophysical research communications

authors： Steiert JG,Crawford RL

更新日期：1986-12-15 00:00:00

abstract：:Elafin, a human skin derived inhibitor of human leukocyte elastase, was tested for inhibitory activity against proteinase 3, an elastin degrading proteinase of neutrophils. The inhibitory activity of elafin was compared with antileukoprotease and eglin C. Elafin proved to be a potent inhibitor of elastin-FITC degradat...

journal_title：Biochemical and biophysical research communications

authors： Wiedow O,Lüademann J,Utecht B

更新日期：1991-01-15 00:00:00

abstract：:A procedure was developed for real-time measurement of the interaction between an archaeal TATA-binding protein (TBP) with stress-gene promoters from the archaeon Methanosarcina mazeii using surface plasmon resonance (SPR), the BIACORE 3000 equipment, and the SA (streptavidin) Sensor Chip. Measurements were based on t...

journal_title：Biochemical and biophysical research communications

authors： Conway de Macario E,Rudofsky UH,Macario AJ

更新日期：2002-11-15 00:00:00

abstract：:Familial exudative vitreoretinopathy (FEVR) is a congenital hereditary, bilateral eye disorder which affects both retina and the vitreous body. As a first step toward the identification of the gene responsible for the X-linked disorder, we report here the results of DNA analyses from the patients and their parents of ...

journal_title：Biochemical and biophysical research communications

authors： Shastry BS,Trese MT

更新日期：1993-06-15 00:00:00

abstract：:During the course of a systematic non-targeting screening of peptide signal molecules in Hydra, we identified a novel myoactive neuropeptide called Hym-176. The primary structure of Hym-176 was determined to be APFIFPGPKVamide. It specifically and reversibly induced contraction of the ectodermal muscle of the hydra bo...

journal_title：Biochemical and biophysical research communications

authors： Yum S,Takahashi T,Koizumi O,Ariura Y,Kobayakawa Y,Mohri S,Fujisawa T

更新日期：1998-07-30 00:00:00

abstract：:Comparison between gel electrophoresis migrations of oligo-alpha-thymidylates and oligo-beta-thymidylates indicates that the migration of alpha-oligonucleotides under native conditions is different from the migration of beta-oligonucleotides when the number of thymines, part of the sequence, is higher than 5. Such dif...

journal_title：Biochemical and biophysical research communications

authors： Bertrand JR,Bazile D,Malvy C,Paoletti J

更新日期：1990-01-15 00:00:00

abstract：:The glutathione S-transferases of human platelets have been compared with those of erythrocytes. Although wide variations in activity were found, in individual subjects, the activity in these cell types was significantly correlated. The enzymes demonstrated similar isoelectric points and electrophoretic mobilities and...

journal_title：Biochemical and biophysical research communications

authors： Rogerson KS,Mitchell D,Lawton A,Ibbotson R,Cotton W,Strange RC

更新日期：1984-07-18 00:00:00

abstract：:The c-Jun N-terminal Kinases (JNKs) play important roles in cell responses to stress or growth factor stimulation. The JNK1α1 isoform shares >90% identity with a predominantly neuronal JNK3α1 isoform, but JNK3α1 also includes a distinctive 38 amino acid N-terminal sequence. To address the outstanding question of the p...

journal_title：Biochemical and biophysical research communications

authors： Chen WK,Yeap YY,Bogoyevitch MA

更新日期：2014-10-24 00:00:00

abstract：:A variety of different forms of cellular stress can cause protein misfolding and aggregation and proteotoxicity. The cytoprotective response to proteotoxicity is termed the integrated stress response and involves 4 distinct serine/threonine protein kinases that converge on the translation initiation factor eIF2α, resu...

journal_title：Biochemical and biophysical research communications

authors： McConkey DJ

更新日期：2017-01-15 00:00:00

abstract：:In a previous genome-wide association study on milk production traits in Chinese Holstein population, we revealed VPS28 gene was highly expressed in mammary gland tissue and a -58C > T mutation in 5'-UTR of it was significantly associated with milk fat content traits. In this study, we explored the effect of this -58C...

journal_title：Biochemical and biophysical research communications

authors： Liu L,Zhang Q

更新日期：2019-03-19 00:00:00