Abstract:
:During the course of a systematic non-targeting screening of peptide signal molecules in Hydra, we identified a novel myoactive neuropeptide called Hym-176. The primary structure of Hym-176 was determined to be APFIFPGPKVamide. It specifically and reversibly induced contraction of the ectodermal muscle of the hydra body column in vivo. However, it had no effect on the ectodermal muscle of the tentacles. The structure-activity relationship analysis showed that the sequence of FIFPGPKVamide is a minimal requirement for the myoactivity. Removal of an amide group from the C-terminus completely abolished the activity. By using the antibody specific to Hym-176, the tissue localization of the peptide in hydra was determined immunohistochemically. The intense immunoreactivity was found in the peduncle nerve cells, indicating that Hym-176 is a neuropeptide.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Yum S,Takahashi T,Koizumi O,Ariura Y,Kobayakawa Y,Mohri S,Fujisawa Tdoi
10.1006/bbrc.1998.8831subject
Has Abstractpub_date
1998-07-30 00:00:00pages
584-90issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(98)98831-3journal_volume
248pub_type
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