Catabolism of pentachlorophenol by a Flavobacterium sp.

abstract：:The ability to simultaneously isolate intact DNA-free RNA, genomic DNA, and proteins from a biological specimen can be useful in cloning genes and analyzing gene expression. Equilibrium density gradient centrifugation with CsCl is a useful tool for fractionating, quantitatively separating, and characterizing RNA, DNA,...

journal_title：Biochemical and biophysical research communications

authors： Zhang H,Chen HT,Glisin V

更新日期：2003-12-05 00:00:00

abstract：:Thymosin beta15 (Tbeta15) is a pleiotropic factor which exerts multiple roles in the development of nervous system and brain diseases. In this study, we found that the expressions of Tbeta15 mRNA and protein were substantially increased in several brain regions including hippocampal formation and cerebral cortex, foll...

journal_title：Biochemical and biophysical research communications

authors： Kim YW,Kim Y,Kim EH,Koh D,Sun W,Kim H

更新日期：2008-07-11 00:00:00

abstract：:Ischemic stroke is a major cause of death and disability worldwide. Hyperneuroinflammation significantly contributes to ischemic stroke. Bromodomain-containing protein 4 (BRD4) is a member of the Bromo and Extra-Terminal (BET) family, and promotes inflammatory response in various types of tissue and cells. Thereby, we...

journal_title：Biochemical and biophysical research communications

authors： Zhou Y,Gu Y,Liu J

更新日期：2019-11-12 00:00:00

abstract：:A suitable antibody for immunoaffinity chromatography is considered to efficiently bind the antigen in the adsorption step and to dissociate the bound antigen with mild changes in the environmental conditions. To develop an effective method for preparing such antibodies, murine antisera elicited by the primary and sec...

journal_title：Biochemical and biophysical research communications

authors： Enomoto A,Kamata N,Nakamura K

更新日期：1994-06-15 00:00:00

abstract：:Gaf-1/Rip11 encoded by the clone KIAA0857 participates in endosomal recycling through the interaction with both gamma-SNAP, a member of the soluble NSF attachment protein family, and a small GTPase, Rab11. Gaf-1/Rip11 and other Rab11-interacting proteins constitute a novel protein family that is involved in the endocy...

journal_title：Biochemical and biophysical research communications

authors： Kawase K,Shibata M,Kawashima H,Hatsuzawa K,Nagahama M,Tagaya M,Tani K

更新日期：2003-04-18 00:00:00

abstract：:VHL tumor suppressor protein contains two domains, alpha and beta. The alpha-domain is involved in the formation of a large protein complex suggested to be involved in ubiquitin-mediated protein degradation. However, the role of the beta-domain, which may recognize the target proteins for protein degradation, remains ...

journal_title：Biochemical and biophysical research communications

authors： Okuda H,Hirai S,Takaki Y,Kamada M,Baba M,Sakai N,Kishida T,Kaneko S,Yao M,Ohno S,Shuin T

更新日期：1999-09-24 00:00:00

abstract：:The recently discovered natural heptadecapeptide nociceptin (orphanin FQ) shares some homology with the opioid peptides but it binds to a distinct receptor type, termed nociceptin receptor. This study demonstrates the presence of specific nociceptin recognition sites in brain membrane fractions of an amphibian, Rana e...

journal_title：Biochemical and biophysical research communications

authors： Benyhe S,Monory K,Farkas J,Tóth G,Guerrini R,Salvadori S,Orosz G,Wollemann M,Borsodi A

更新日期：1999-07-14 00:00:00

abstract：:Periplasmic proteins of Gram-negative bacteria like Escherichia coli are subjected to immediate affect of environmental fluctuation that may unfold proteins, due to the permeability of the outer membrane to small molecules. They are thus supposedly protected by certain molecular chaperones. Nevertheless, no homologues...

journal_title：Biochemical and biophysical research communications

authors： Liu Y,Fu X,Shen J,Zhang H,Hong W,Chang Z

更新日期：2004-04-09 00:00:00

abstract：:The transcription regulator SNAI1 triggers a transcriptional program leading to epithelial to mesenchymal transition (EMT), providing epithelial cells with mesenchymal features and invasive properties during embryonic development and tumor progression. To identify early transcriptional changes occurring during SNAI1-i...

journal_title：Biochemical and biophysical research communications

authors： Vetter G,Le Béchec A,Muller J,Muller A,Moes M,Yatskou M,Al Tanoury Z,Poch O,Vallar L,Friederich E

更新日期：2009-08-07 00:00:00

abstract：:In rat adrenal dispersed cells, both vanadyl and vanadate inhibited ACTH-stimulated steroidogenesis and the formation of cAMP, whereas these compounds did not inhibit the cAMP-dependent steroidogenesis. Then, the membrane fraction was prepared and activated by various secretagogues including ACTH, Gpp(NH)p, GTP gamma ...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Kimura T

更新日期：1985-08-15 00:00:00

abstract：:To study the role of the cytoplasmic domain and particularly the tyrosine residues of the erythropoietin receptor (EpoR) in erythroid differentiation of human primary stem cells, we infected cord blood-derived CD34+ cells with retroviruses encoding chimeric receptors containing the extracellular domain of the prolacti...

journal_title：Biochemical and biophysical research communications

authors： Fichelson S,Chrétien S,Rokicka-Piotrowicz M,Bouhanik S,Gisselbrecht S,Mayeux P,Lacombe C

更新日期：1999-03-24 00:00:00

abstract：:Cisplatin plays a key role in treating small cell lung cancer (SCLC); however, the rapid development of cisplatin resistance limits its treatment effect. The detailed mechanisms of cisplatin-resistance, particularly in SCLC, remain unclear. We analyzed the differentially expressed genes (DEGs) between cisplatin-resist...

journal_title：Biochemical and biophysical research communications

authors： Ma K,Li S,Huo X,Guo M,Du X,Li C,Liu X,Lv J,Chen Z

更新日期：2020-12-10 00:00:00

abstract：:Thioredoxin (TRX) is one of major components of thiol reducing systems. To investigate the molecular mechanism of TRX function in the lung tissue, we screened a human lung epithelial cell cDNA library for TRX-binding protein by yeast two-hybrid systems. We isolated a plasmid containing C-propeptide region of human pro...

journal_title：Biochemical and biophysical research communications

authors： Matsumoto K,Masutani H,Nishiyama A,Hashimoto S,Gon Y,Horie T,Yodoi J

更新日期：2002-07-19 00:00:00

abstract：:The prevalence of nonalcoholic fatty liver disease (NAFLD) is high and increasing throughout the world. The intense sterile inflammation stemming from steatosis plays a significant role in the development of NAFLD, but the mechanism is unclear. We found that the NLRC4 inflammasome was activated in an in vitro cell mod...

journal_title：Biochemical and biophysical research communications

authors： Chen Y,Ma K

更新日期：2019-04-09 00:00:00

abstract：:Hemangioma is a benign tumor derived from abnormal blood vessel growth. Unlike other vascular tumor counterparts, a hemangioma is known to proliferate during its early stage but it is followed by a stage of involution where regression of the tumor occurs. The critical onset leading to the involution of hemangioma is c...

journal_title：Biochemical and biophysical research communications

authors： Kim KJ,Yun JH,Heo JI,Lee EH,Min HS,Choi TH,Cho CH

更新日期：2014-11-14 00:00:00

abstract：:To investigate molecular mechanisms linking inflammation with neurodegeneration, we treated neuronal cultures with prostaglandins (PGs), which are mediators of inflammation. PGA1, D2, J2, and Delta12-PGJ2, but not PGE2, reduced the viability and raised the levels of ubiquitinated proteins in the neuronal cells. PGJ2 a...

journal_title：Biochemical and biophysical research communications

authors： Li Z,Melandri F,Berdo I,Jansen M,Hunter L,Wright S,Valbrun D,Figueiredo-Pereira ME

更新日期：2004-07-09 00:00:00

abstract：:The Ca(2+)-binding proteins regulate a number of cellular and extracellular activities and deregulations of S100 gene expression are associated with several human diseases. For example, S100A7 is upregulated in psoriatic skin, implicating a link with psoriasis, a chronic inflammatory dermatosis. We purified human S100...

journal_title：Biochemical and biophysical research communications

authors： Bürgisser DM,Siegenthaler G,Kuster T,Hellman U,Hunziker P,Birchler N,Heizmann CW

更新日期：1995-12-05 00:00:00

abstract：:The initiating factors in primary, idiopathic osteoarthritis are unknown, the characteristic bone and cartilage changes being late features of the disease. We have proposed that biochemical cruciate ligament alteration may be important in early osteoarthritis by mediating loading consequences on the bone and cartilage...

journal_title：Biochemical and biophysical research communications

authors： Anderson-MacKenzie JM,Billingham ME,Bailey AJ

更新日期：1999-05-19 00:00:00

abstract：:Kaiso was previously described as a methylated DNA-binding protein and a transcription repressor interacting with the corepressor protein complex NCoR. In the current study, we show that generation-3 Kaiso knockout mice show a phenotype of splenomegaly and large diffused germinal centers (GC). In the spleens of Kaiso ...

journal_title：Biochemical and biophysical research communications

authors： Koh DI,Yoon JH,Kim MK,An H,Kim MY,Hur MW

更新日期：2013-12-13 00:00:00

abstract：:The Cdc6 protein is an essential regulator for initiation of DNA replication. Following the G1/S transition, Cdc6 is degraded through a ubiquitin-mediated proteolysis pathway. In this study, we tagged Cdc6 with green fluorescent protein (GFP) and used site-specific mutations to study the regulation of Cdc6 localizatio...

journal_title：Biochemical and biophysical research communications

authors： Luo KQ,Elsasser S,Chang DC,Campbell JL

更新日期：2003-07-11 00:00:00

abstract：:Extracellular DNA (eDNA) is a structural component of the polymeric matrix of biofilms from different species. Different mechanisms for DNA release have been proposed including lysis of cells, lysis of DNA-containing vesicles, and DNA secretion. Here, a genome-wide screen of 3985 non-lethal mutations was performed to ...

journal_title：Biochemical and biophysical research communications

authors： Sanchez-Torres V,Maeda T,Wood TK

更新日期：2010-10-15 00:00:00

abstract：:Stimulation of DNA synthesis by EGF was compared in cultured periportal and perivenous hepatocyte populations. Periportal hepatocytes responded to EGF more sensitive (IC50-values 20 vs 75 ng/ml) and with a higher maximal stimulation (420 vs 290%) than perivenous hepatocytes with respect to both [3H]thymidine incorpora...

journal_title：Biochemical and biophysical research communications

authors： Gebhardt R,Jonitza D

更新日期：1991-12-31 00:00:00

abstract：:Huntington's disease (HD) is caused by mutations that expand a polyglutamine region in the amino-terminal domain of Huntingtin (Htt), leading to the accumulation of intracellular inclusions and progressive neurodegeneration. Recent reports indicate the engagement of endoplasmic reticulum (ER) stress responses in human...

journal_title：Biochemical and biophysical research communications

authors： Zuleta A,Vidal RL,Armentano D,Parsons G,Hetz C

更新日期：2012-04-13 00:00:00

abstract：:GTP gamma S-dependent phospholipase D (PLD) activity time-dependently increased during differentiation of rat C6 glioma cells to astrocytic phenotypes induced by dibutyryl cyclic AMP (dbcAMP)/theophylline. The changes in PLD mRNA level were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method us...

journal_title：Biochemical and biophysical research communications

authors： Yoshimura S,Nakashima S,Ohguchi K,Sakai H,Shinoda J,Sakai N,Nozawa Y

更新日期：1996-08-14 00:00:00

abstract：:Hypoxic preconditioning is a well-known strategy to improve the survival and therapeutic potential of stem cells against various challenges including hemodynamic and neurohormonal modulations. However, the mechanism involved in hypoxia-induced benefits on stem cells is still ambiguous. In pathological hypertension, th...

journal_title：Biochemical and biophysical research communications

authors： Lai CH,Barik P,Hsieh DJ,Day CH,Ho TJ,Chen RJ,Kuo WW,Padma VV,Shibu MA,Huang CY

更新日期：2020-11-12 00:00:00

abstract：:DNA methylation plays a central role in the epigenetic regulation of gene expression during development and progression of cancer diseases. The inheritance of specific DNA methylation patterns are acquired in the early embryo and are specifically maintained after cellular replication via the DNA methyltransferase 1 (D...

journal_title：Biochemical and biophysical research communications

authors： Lavoie G,St-Pierre Y

更新日期：2011-06-03 00:00:00

abstract：:Despite numerous studies on detergent-induced solubilization of membranes and on the underlying mechanisms associated with this process, very little is known regarding the selectivity of detergents for lipids during their extraction from membranes. To get insights about this phenomenon, solubilization of model bilayer...

journal_title：Biochemical and biophysical research communications

authors： Azouz M,Therrien A,Buré C,Tokarski C,Lecomte S,Lafleur M

更新日期：2020-10-15 00:00:00

abstract：:Protein markers induced by hormones are the necessary probes to study hormone regulation of gene expression. We have previously shown that ACTH was able to induce one of these markers in the cytosol of the rat adrenal: protein E (Dazord et al, Biochem. J., 176, 233-239, 1978). In this paper by using adrenal mRNA from ...

journal_title：Biochemical and biophysical research communications

authors： Langlois-Gallet D,Dazord A

更新日期：1984-03-30 00:00:00

abstract：:The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) was investigated at pH 6.2 and 21.0 degrees C. The results are consistent with the minimum three-step catalytic mechanism. The acylation step is rate limiting for human...

journal_title：Biochemical and biophysical research communications

authors： Balliano G,Milla P,Giordano C,Gallina C,Coletta M,Menegatti E,Rizzi M,Bolognesi M,Ascenzi P

更新日期：1996-08-14 00:00:00

abstract：:L-amino acids represent the most common amino acid form, most notably as protein residues, whereas D-amino acids, despite their rare occurrence, play significant roles in many biological processes. Amino acid racemases are enzymes that catalyze the interconversion of L- and/or D-amino acids. McyF is a pyridoxal 5'-pho...

journal_title：Biochemical and biophysical research communications

authors： Cao DD,Zhang CP,Zhou K,Jiang YL,Tan XF,Xie J,Ren YM,Chen Y,Zhou CZ,Hou WT

更新日期：2019-07-05 00:00:00