Regulation of the localization and stability of Cdc6 in living yeast cells.

abstract：:Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis (MTB), was originally identified as an immuno-dominant antigen and later found to be a major membrane protein. In vitro studies show that Hsp16.3 exists as nonamers and undergoes dynamic dissociation/re-association equilibrium in solutions. Neverthele...

journal_title：Biochemical and biophysical research communications

authors： Zhang H,Fu X,Jiao W,Zhang X,Liu C,Chang Z

更新日期：2005-05-20 00:00:00

abstract：:Long non-coding RNAs (LncRNAs) have played very important roles in the malignancy behaviors of hepatocellular carcinoma (HCC). Linc-cdh4-2 (TCONS_00027978) is a novel LncRNA that has been identified in HCC tissues from our previous study. Overexpression of linc-cdh4-2 in HCC cell lines (SK-Hep-1 and Huh7) significantl...

journal_title：Biochemical and biophysical research communications

authors： Gao Y,Wang G,Zhang C,Lin M,Liu X,Zeng Y,Liu J

更新日期：2016-11-18 00:00:00

abstract：:We investigated the expression of myocilin in the optic nerve head of porcine eyes by Western blotting and immunohistochemical staining. Myocilin was localized in the nucleus, centrosome, glial filament, mitochondria, and some parts of the cell membranes of the astrocytes. Myocilin was also detected at the edge-feet p...

journal_title：Biochemical and biophysical research communications

authors： Noda S,Mashima Y,Obazawa M,Kubota R,Oguchi Y,Kudoh J,Minoshima S,Shimizu N

更新日期：2000-10-05 00:00:00

abstract：:Xanthine oxidase (XO) was found to convert nitric oxide (NO* ) released from spermine-NONOate to nitroxyl (HNO), the one-electron reduction product of NO*, in the presence of its substrate hypoxanthine under anaerobic conditions. Under these conditions, XO lost its activity. Upon aerobic incubation of XO with its subs...

journal_title：Biochemical and biophysical research communications

authors： Saleem M,Ohshima H

更新日期：2004-03-05 00:00:00

abstract：:A GSH-dependent dehydroascorbate reductase (EC 1.8.5.1) was purified to homogeneity from human erythrocytes. The enzyme was a monomer of 32 kDa and was purified 133-fold from a crude DEAE-Sepharose fraction with a 25% yield. The reduced protein had a pI of 5.1 as judged by isoelectric focusing. Kinetic analysis gave a...

journal_title：Biochemical and biophysical research communications

authors： Xu DP,Washburn MP,Sun GP,Wells WW

更新日期：1996-04-05 00:00:00

abstract：:Mutations in the Treacher Collins syndrome gene, TCOF1, cause a disorder of craniofacial development. We manipulated the levels of Tcof1 and its protein treacle in a murine neuroblastoma cell line to identify downstream changes in gene expression using a microarray platform. We identified a set of genes that have simi...

journal_title：Biochemical and biophysical research communications

authors： Mogass M,York TP,Li L,Rujirabanjerd S,Shiang R

更新日期：2004-12-03 00:00:00

abstract：:A number of cyclohexenyl chalcone Diels-Alder natural products possess promising biological properties including strong cytotoxicity in various human cancer cells. Herein, we show that natural products in this class including panduratin A and nicolaioidesin C inhibit cysteine cathepsins as indicated by protease profil...

journal_title：Biochemical and biophysical research communications

authors： Deb Majumdar I,Devanabanda A,Fox B,Schwartzman J,Cong H,Porco JA Jr,Weber HC

更新日期：2011-12-16 00:00:00

abstract：:Topical application of hexadecane has been shown to induce hyperproliferation and hyperkeratosis in rodent skin. The application of hexadecane to epidermis from the backs of piglets less than 1 week old resulted in a rapid biphasic-rise in the level of ornithine decarboxylase (ODC) activity. The second phase of the el...

journal_title：Biochemical and biophysical research communications

authors： Owusu S,Cunliffe WJ,Wood EJ

更新日期：1989-06-30 00:00:00

abstract：:Defects in neurogenesis and neuronal differentiation in the fetal brain of Down syndrome (DS) patients lead to the apparent neuropathological abnormalities and contribute to the phenotypic characters of mental retardation, and premature development of Alzheimer's disease, those being the most common phenotype in DS. I...

journal_title：Biochemical and biophysical research communications

authors： Wang CC,Kadota M,Nishigaki R,Kazuki Y,Shirayoshi Y,Rogers MS,Gojobori T,Ikeo K,Oshimura M

更新日期：2004-02-06 00:00:00

abstract：:Activation of the extracellular calcium-sensing receptor (CaR) stimulates mitogen-activated protein kinases to upregulate the synthesis and secretion of parathyroid hormone related peptide (PTHrP) from cells expressing the CaR heterologously or endogenously. The current experiments demonstrate that this occurs because...

journal_title：Biochemical and biophysical research communications

authors： MacLeod RJ,Yano S,Chattopadhyay N,Brown EM

更新日期：2004-07-23 00:00:00

abstract：:Gastric epithelial cells (GES-1) stimulated by Helicobacter pylori (H. pylori) would affect the expression of related genes and induce the immune response of the cells. Abnormal methylation of DNA was one of the main causes. The aim of this study was to investigate phosphoinositol-3-kinase adaptor protein 1(PIK3AP1), ...

journal_title：Biochemical and biophysical research communications

authors： Zhang A,Yan S,Cao M,Wu D,Zhou J,Yu Z,Wu M,Liu Y,Lu S,Hu G,Zhao J

更新日期：2020-03-26 00:00:00

abstract：:To investigate the possible correlation between genotype and phenotype of epilepsy, we analyzed the voltage-gated sodium channel alpha1-subunit (SCN1A) gene, beta1-subunit (SCN1B) gene, and gamma-aminobutyric acid(A) receptor gamma2-subunit (GABRG2) gene in DNAs from peripheral blood cells of 29 patients with severe m...

journal_title：Biochemical and biophysical research communications

authors： Ohmori I,Ouchida M,Ohtsuka Y,Oka E,Shimizu K

更新日期：2002-07-05 00:00:00

abstract：:Myeloid differentiation protein 2 (MD-2) is a co-receptor of toll-like receptor 4 (TLR4) for innate immunity. Here, we delineated a new mechanism of 1-dehydro-10-gingerdione (1D10G), one of pungent isolates from ginger (Zingiber officinale), in the suppression of lipopolysaccharide (LPS)-induced gene expression of inf...

journal_title：Biochemical and biophysical research communications

authors： Park SH,Kyeong MS,Hwang Y,Ryu SY,Han SB,Kim Y

更新日期：2012-03-23 00:00:00

abstract：:Little is known as to the structural requirements of the acyl side chain for interaction of acylglycerols with monoacylglycerol lipase (MAGL), the enzyme chiefly responsible for the metabolism of the endocannabinoid 2-arachidonoylglycerol (2-AG) in the brain. In the present study, a series of twelve analogues of 1-AG ...

journal_title：Biochemical and biophysical research communications

authors： Vandevoorde S,Saha B,Mahadevan A,Razdan RK,Pertwee RG,Martin BR,Fowler CJ

更新日期：2005-11-11 00:00:00

abstract：:Metallodaunomycin has been shown to cleave DNA only in the presence of oxygen, a reducing agent and a metal ion under reaction conditions similar to those used for the cuprous-phenanthroline complex. The intermediacy of 02-. and H2O2 has been substantiated by experiments with superoxide dismutase and catalase, respect...

journal_title：Biochemical and biophysical research communications

authors： Mariam YH,Glover GP

更新日期：1986-04-14 00:00:00

abstract：:Purified Human Immunodeficiency Virus (HIV) was solubilized in octylglucopyranoside. After centrifugation, the supernatant was added to lipid-detergent mixed micelles. Formation of virosomes occurred during overnight dialysis. Centrifugation on a continuous glycerol gradient showed that envelope glycoproteins (gp120 a...

journal_title：Biochemical and biophysical research communications

authors： Cornet B,Vandenbranden M,Cogniaux J,Giurgea L,Dekegel D,Ruysschaert JM

更新日期：1990-02-28 00:00:00

abstract：:SUMOylation, a post-translational modification of lysine residues by small ubiquitin-like modifier (SUMO) proteins, has been implicated in the pathogenesis of neurodegenerative disorders including Alzheimer's disease (AD), and in neuron- and astrocyte-specific physiological functions. Global SUMOylation is increased i...

journal_title：Biochemical and biophysical research communications

authors： Maruyama T,Wada H,Abe Y,Niikura T

更新日期：2018-06-02 00:00:00

abstract：:Although TRPV1 and TRPM8 agonists (vanilloid capsaicin and menthol, respectively) at high concentrations inhibit action potential conduction, it remains to be unknown whether TRPA1 agonists have a similar action. The present study examined the actions of TRPA1 agonists, cinnamaldehyde (CA) and allyl isothiocyanate (AI...

journal_title：Biochemical and biophysical research communications

authors： Matsushita A,Ohtsubo S,Fujita T,Kumamoto E

更新日期：2013-04-26 00:00:00

abstract：:The effect of guanine nucleotides on activation of the O2-. generating oxidase in a cell free system consisting of bovine neutrophils membranes, cytosol and arachidonic acid has been studied. In a complete system, GTP-gamma-S was stimulatory and GDP-beta-S inhibitory. When cytosol was omitted, both nucleotides acted a...

journal_title：Biochemical and biophysical research communications

authors： Doussiere J,Pilloud MC,Vignais PV

更新日期：1988-05-16 00:00:00

abstract：:The NS3 serine protase of Hepatitis C virus (HCV) requires NS4A protein as a cofactor for efficient cleavage at four sites in the nonstructural region. The cofactor activity has been mapped to the central hydrophobic region (aa 22-34) of this 54-amino-acid NS4A protein, and site-directed mutagenesis has identified alt...

journal_title：Biochemical and biophysical research communications

authors： Butkiewicz NJ,Yao N,Wright-Minogue J,Zhang R,Ramanathan L,Lau JY,Hong Z,Dasmahapatra B

更新日期：2000-01-07 00:00:00

abstract：:A system for the expression and purification of soluble VP8*, part of the human rotavirus (HRV) spike protein, was established by expressing VP8* as a fusion protein with glutathione S-transferase (GST). VP8 cDNA, from the Wa strain of HRV, was prepared by RT-PCR, cloned into a pUC18 plasmid, and inserted into a pGEX-...

journal_title：Biochemical and biophysical research communications

authors： Kovacs-Nolan J,Sasaki E,Yoo D,Mine Y

更新日期：2001-04-20 00:00:00

abstract：:In epithelial cells progression through the G1 phase of the cell cycle and preparing the cell for the S phase is regulated by cyclin D1-cdk4. Cells that express the retinoblastoma protein (pRb) are dependent on cyclin D1-cdk4 activity for their proliferation while cells that do not express pRb are not. Overexpression ...

journal_title：Biochemical and biophysical research communications

authors： Kent LL,Hull-Campbell NE,Lau T,Wu JC,Thompson SA,Nori M

更新日期：1999-07-14 00:00:00

abstract：:Based on the high level of identity among human, mouse, and rat MRP1 protein sequence, we produced a specific polyclonal antibody (MRP1-A23) against a synthetic polypeptide covering the C-terminus of the human protein. Western blot analysis showed a reactivity against human MRP1 similar to that obtained with the monoc...

journal_title：Biochemical and biophysical research communications

authors： Fernetti C,Pascolo L,Podda E,Gennaro R,Stebel M,Tiribelli C

更新日期：2001-11-09 00:00:00

abstract：:IL-13 mediates its effects through a complex receptor system including IL-4Ralpha and a functional IL-13Ralpha1. IL-13 has been reported to have no effects on mouse B cells due to a lack of receptor expression. However, on human B cells a functional IL-13Ralpha1 has been described. Here, we identified the rat IL-13Ral...

journal_title：Biochemical and biophysical research communications

authors： Pierrot C,Beniguel L,Bègue A,Khalife J

更新日期：2001-10-05 00:00:00

abstract：:The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca(2+) concentration ([Ca(2+)](i)) using CaR-transfected human embryonic kidney (HEK)-293...

journal_title：Biochemical and biophysical research communications

authors： McLarnon S,Holden D,Ward D,Jones M,Elliott A,Riccardi D

更新日期：2002-09-13 00:00:00

abstract：:Lipoproteins are important biological components. However, they have few convenient fluorescent labelling probes currently reported, and their physiological reliability can be questioned. We compared the association of two fluorescent cholesterol derivatives, 22-nitrobenzoxadiazole-cholesterol (NBD-Chol) and 21-methyl...

journal_title：Biochemical and biophysical research communications

authors： Gaibelet G,Tercé F,Bertrand-Michel J,Allart S,Azalbert V,Lecompte MF,Collet X,Orlowski S

更新日期：2013-11-01 00:00:00

abstract：:The post-transcriptional regulation of gene expression plays an important role in many essential biological processes. The RNA decapping enzyme Dcp2 is a crucial enzyme involved in RNA degradation. Dcp2 proteins are highly expressed in the testis and brain in adult mice. This study aimed to investigate the in vivo fun...

journal_title：Biochemical and biophysical research communications

authors： Li RM,Zhang MN,Tang QY,Song MG

更新日期：2020-05-28 00:00:00

abstract：:Tissue nicotinamide adenine dinucleotide (NAD+) decline has been implicated in aging. We have recently identified CD38 as a central regulator involved in tissue NAD+ decline during the aging process. CD38 is an ecto-enzyme highly expressed in endothelial and inflammatory cells. To date, the mechanisms that regulate CD...

journal_title：Biochemical and biophysical research communications

authors： Chini C,Hogan KA,Warner GM,Tarragó MG,Peclat TR,Tchkonia T,Kirkland JL,Chini E

更新日期：2019-05-28 00:00:00

abstract：:Intestinal epithelial cells (IECs) must be tolerant of the large number of commensal bacteria inhabiting the intestinal tract to avoid excessive inflammatory reactions. Toll-interacting protein (Tollip), a negative regulator of Toll-like receptor signaling, is known to be expressed at high levels in IECs, and to there...

journal_title：Biochemical and biophysical research communications

authors： Sugi Y,Takahashi K,Nakano K,Hosono A,Kaminogawa S

更新日期：2011-09-09 00:00:00

abstract：:Ligand-blotting and dot-blotting procedures were used to investigate the binding of [125I]-heparin to apolipoprotein E, its thrombin fragments E22 (residues 1-191) and E12 (residues 192-299), and to nine apolipoprotein E synthetic fragments. E22 and E12 bound [125I] heparin indicating multiple heparin-binding domains....

journal_title：Biochemical and biophysical research communications

authors： Cardin AD,Hirose N,Blankenship DT,Jackson RL,Harmony JA,Sparrow DA,Sparrow JT

更新日期：1986-01-29 00:00:00