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Preparation of an antibody recognizing both human and rodent MRP1.

Abstract：

:Based on the high level of identity among human, mouse, and rat MRP1 protein sequence, we produced a specific polyclonal antibody (MRP1-A23) against a synthetic polypeptide covering the C-terminus of the human protein. Western blot analysis showed a reactivity against human MRP1 similar to that obtained with the monoclonal QCRL1 antibody. Differently from other available antibodies against human MPR1, MRP1-A23 also detected both rat and mouse MRP1. No cross-reactivity was observed with either human or mouse MRP2 while MRP1-A23 weakly cross-reacted with rat MRP2 in the protein region ranging from 1512 to 1533 amino acids. These data indicate that MRP1-A23 allows specific MRP1 detection in both human and rodent tissues and may provide an important tool in the study of MRP1 expression and function in both experimental and clinical materials.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Fernetti C,Pascolo L,Podda E,Gennaro R,Stebel M,Tiribelli C

doi

10.1006/bbrc.2001.5885

subject

Has Abstract

pub_date

2001-11-09 00:00:00

pages

1064-8

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(01)95885-1

journal_volume

288

pub_type

杂志文章

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