Purification and characterization of a glutathione dependent dehydroascorbate reductase from human erythrocytes.

abstract：:The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome coronavirus (SARS-CoV) plays key roles in viral replication and is an attractive target for anti-SARS drug discovery. In this report, a fluorescence resonance energy transfer (FRET)-based method was developed to assess the proteolytic activity of SAR...

journal_title：Biochemical and biophysical research communications

authors： Kuang WF,Chow LP,Wu MH,Hwang LH

更新日期：2005-06-17 00:00:00

abstract：:The polyketide synthases found in a variety of plants and fungi provide a varied source of biologically active compounds of pharmacological and medicinal interest. Stilbene synthase and chalcone synthase catalyze the formation of a common tetraketide intermediate, but use different cyclization mechanisms to produce di...

journal_title：Biochemical and biophysical research communications

authors： Healy EF,Cervantes L,Nabona B,Williams J

更新日期：2018-03-18 00:00:00

abstract：:Aging is associated with extensive cognitive impairments, although the biochemical and physiological basis of these deficits are unknown. As the hippocampus plays a vital role in cognitive functions, we have selected this tissue to analyze changes in gene expression at two different ages. Array technology is utilized ...

journal_title：Biochemical and biophysical research communications

authors： Kumar VB,Franko MW,Farr SA,Armbrecht HJ,Morley JE

更新日期：2000-06-16 00:00:00

abstract：:Stress activated c-Jun N-terminal Kinase-3 (JNK3) has been reported to act on mitochondrion to promote neuronal cell death. Phosphorylation of mitochondrial Voltage-Dependent Anion Channel (VDAC) plays an important role in mitochondria-mediated cell death. Keeping these in view phosphorylation of rat brain VDAC by JNK...

journal_title：Biochemical and biophysical research communications

authors： Gupta R,Ghosh S

更新日期：2015-03-27 00:00:00

abstract：:Adenovirus vector is one of the most widely used vectors in gene therapy applications for the treatment of diverse human diseases including cancer. In this study, we showed that infection with E1E3-deleted recombinant human adenovirus serotype 5 reduced human telomerase reverse transcriptase (hTERT) mRNA levels in hep...

journal_title：Biochemical and biophysical research communications

authors： Lee CH,Kim HW,Kim T,Lee SW

更新日期：2015-03-20 00:00:00

abstract：:Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-stre...

journal_title：Biochemical and biophysical research communications

authors： Kim JH,Lee CS,Kim BG

更新日期：2005-05-27 00:00:00

abstract：:Synthesis of melanin starts from the conversion of L-tyrosine to 3,4-dihydroxyphenylalanine (L-dopa) and then the oxidation of L-dopa yields dopaquinone by tyrosinase. Therefore, tyrosinase inhibitors have been established as important constituents of depigmentation agents. Recently, polyhydroxystilbene compounds, whi...

journal_title：Biochemical and biophysical research communications

authors： Ohguchi K,Tanaka T,Kido T,Baba K,Iinuma M,Matsumoto K,Akao Y,Nozawa Y

更新日期：2003-08-08 00:00:00

abstract：:Virosomes as membranous vesicles with viral fusion protein in their membrane are versatile vehicles for cargo delivery. The vesicular stomatitis virus glycoprotein (VSV-G) is a common fusogenic protein used in virosome preparation. This glycoprotein has been used in liposomal systems so far, but in this study, we have...

journal_title：Biochemical and biophysical research communications

authors： Asadikaram G,Poustforoosh A,Pardakhty A,Torkzadeh-Mahani M,Nematollahi MH

更新日期：2021-01-01 00:00:00

abstract：:4-Nonylphenol (NP) is reported to have estrogenic activity; however, its influence on cytokine production or immune system function remains unclear. In this study we investigated the effects of NP on the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), and on the level of inducible nitric o...

journal_title：Biochemical and biophysical research communications

authors： You HJ,Choi CY,Jeon YJ,Chung YC,Kang SK,Hahm KS,Jeong HG

更新日期：2002-06-21 00:00:00

abstract：:Lung cancer accounts for the highest death rate among cancers worldwide, with most patients being diagnosed with non-small cell lung cancer (NSCLC), urging more effective therapies. We report that JK273, a pyrrolo[2,3-d]pyrimidine analog, which inhibits α4 integrin signaling, showed a selective cytotoxic effect agains...

journal_title：Biochemical and biophysical research communications

authors： Lu TN,Ganganna B,Pham TT,Vo AV,Lu TP,Nguyen HT,Nguyen MT,Huynh PN,Truong NT,Lee J

更新日期：2017-09-16 00:00:00

abstract：:Rett syndrome is a neurodevelopmental disorder caused by Mecp2 gene mutations. In RTT patients and Mecp2-null (Mecp2(-/Y)) mice, norepinephrine (NE) content drops significantly, which may play a role in breathing arrhythmia, sleep disorders and sudden death. However, the underlying mechanisms for the NE defect are not...

journal_title：Biochemical and biophysical research communications

authors： Zhang X,Su J,Rojas A,Jiang C

更新日期：2010-04-02 00:00:00

abstract：:Eosinophil cationic protein (ECP) is a toxin secreted by activated human eosinophils. The properties of mature ECP have been well studied but those of the signal peptide of ECP (ECPsp) are not clear. In this study, several chimeric proteins containing N-terminal fusion of ECPsp were generated, and introduced into Esch...

journal_title：Biochemical and biophysical research communications

authors： Wu CM,Chang MD

更新日期：2004-09-17 00:00:00

abstract：:Maternally inherited diabetes and deafness (MIDD) is a mitochondrial syndrome characterized by the onset of sensorineural hearing loss and diabetes in adults. Some patients may have other additional clinical features common in mitochondrial disorders such as pigmentary retinopathy, ptosis, cardiomyopathy, myopathy and...

journal_title：Biochemical and biophysical research communications

authors： Mezghani N,Mnif M,Mkaouar-Rebai E,Kallel N,Charfi N,Abid M,Fakhfakh F

更新日期：2013-02-22 00:00:00

abstract：:Platelet activation (cytosolic [Ca2+] increase, aggregation and ATP secretion) was induced with A1F-4. This agent presumably interacts with a G protein which appears to mediate the coupling of the receptors for Ca mobilizing hormones and phospholipase C. All the A1F-4 evoked responses were inhibited by treatment with ...

journal_title：Biochemical and biophysical research communications

authors： Doni MG,Deana R,Bertoncello S,Zoccarato F,Alexandre A

更新日期：1988-11-15 00:00:00

abstract：:The formation of inactive complexes in excess molar amounts of human cathepsins H and L with their protein inhibitors human stefin A, human stefin B and chicken cystatin at pH 5.6 has been shown by measurement of enzyme activity coupled with reverse-phase HPLC not to involve covalent cleavage of the inhibitors. Inhibi...

journal_title：Biochemical and biophysical research communications

authors： Lenarcic B,Kos J,Dolenc I,Lucovnik P,Krizaj I,Turk V

更新日期：1988-07-29 00:00:00

abstract：:Despite numerous studies on detergent-induced solubilization of membranes and on the underlying mechanisms associated with this process, very little is known regarding the selectivity of detergents for lipids during their extraction from membranes. To get insights about this phenomenon, solubilization of model bilayer...

journal_title：Biochemical and biophysical research communications

authors： Azouz M,Therrien A,Buré C,Tokarski C,Lecomte S,Lafleur M

更新日期：2020-10-15 00:00:00

abstract：:The effect of glucocorticoid hormones on the protein responsible for both nucleotide pyrophosphatase (EC 3.6.1.9) and alkaline phosphodiesterase I (EC 3.1.4.1) activities was examined in murine MOPC 315 plasmacytoma cells. Incubation of these cells with dexamethasone resulted in parallel increases in pyrophosphatase a...

journal_title：Biochemical and biophysical research communications

authors： Rebbe NF,Hickman S

更新日期：1991-03-15 00:00:00

abstract：:Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparations from rat superior...

journal_title：Biochemical and biophysical research communications

authors： Kimura K,Tsuda K,Moriwaki C,Kawabe T,Hamada M,Obana M,Baba A,Hano T,Nishio I

更新日期：2002-06-07 00:00:00

abstract：:Protein phosphatase 2Cs (PP2Cs) have been referred to act as negative modulators of the protein kinase pathways involved in different environmental stress responses and developmental processes. In Arabidopsis, PP2Cs have been extensively studied and some are known to negatively regulate abscisic acid signaling. In ric...

journal_title：Biochemical and biophysical research communications

authors： Amir Hossain M,Ahn CH,Lee IS,Jeon JS,An G,Park PB

更新日期：2018-09-10 00:00:00

abstract：:SMCX/JARID1C was recently shown to be a histone H3 lysine 4 (H3K4) demethylase. Here, we have identified an SMCX isoform that predominantly resides in the cytoplasm, but still efficiently demethylates trimethylated H3K4. SMCX requires several functional domains for its demethylase activity and is also capable of formi...

journal_title：Biochemical and biophysical research communications

authors： Kim TD,Shin S,Janknecht R

更新日期：2008-02-08 00:00:00

abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

journal_title：Biochemical and biophysical research communications

authors： Tian H,Hou X,Liu X

更新日期：2013-02-08 00:00:00

abstract：:A significant percentage of the methyltransferasome in Saccharomyces cerevisiae and higher eukaryotes is devoted to methylation of the translational machinery. Methylation of the RNA components of the translational machinery has been studied extensively and is important for structure stability, ribosome biogenesis, an...

journal_title：Biochemical and biophysical research communications

authors： Al-Hadid Q,White J,Clarke S

更新日期：2016-02-12 00:00:00

abstract：:The effect of musk xylene on contents of both cytochrome P-450IA1 and cytochrome P-450IA2 in rat liver was investigated using Western blotting analysis. Rats were treated i.p. for five consecutive days with either 50, 100 or 200 mg musk xylene/kg body weight. Musk xylene increased both total cytochrome P-450 and cytoc...

journal_title：Biochemical and biophysical research communications

authors： Iwata N,Minegishi K,Suzuki K,Ohno Y,Kawanishi T,Takahashi A

更新日期：1992-04-15 00:00:00

abstract：:Angiopoietin-2 (Ang-2) plays an important role in destabilizing vessels for angiogenesis, however its transcriptional regulation has not been determined. Here we isolated about 3.2kb of the 5' upstream of the human Ang-2 gene and further characterized the transcriptional regulation of Ang-2. We found 10 Ets binding si...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa Y,Abe M,Yamazaki T,Niizeki O,Shiiba K,Sasaki I,Sato Y

更新日期：2004-03-26 00:00:00

abstract：:The status of Ca(2+)-channels and adrenoceptors in the hind leg skeletal muscle was examined in rats 8 weeks after inducing diabetes by an intravenous injection of streptozotocin (65 mg/kg). Scatchard plot analysis of the data on specific binding of 3H-nitrendipine with crude membranes from diabetic muscle revealed an...

journal_title：Biochemical and biophysical research communications

authors： Lee SL,Dhalla NS

更新日期：1992-04-15 00:00:00

abstract：:Bovine rhodopsin was recombined with various phospholipids in which the lipid acyl chain composition was held constant at that of egg phosphatidylcholine (PC), while the identity of the headgroups was varied. The ratio of MII/MI produced in the recombinant membrane vesicles by an actinic flash was studied as a functio...

journal_title：Biochemical and biophysical research communications

authors： Gibson NJ,Brown MF

更新日期：1991-04-30 00:00:00

abstract：:The cytochrome P-450 catalyzing 26-hydroxylation of C27-steroids (cytochrome P-450(26] was purified from rabbit liver mitochondria. The specific content of the cytochrome P-450 was 13.6 nmol per mg of protein and the 26-hydroxylase activity towards 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol was 31,300 pmol/nmo...

journal_title：Biochemical and biophysical research communications

authors： Dahlbäck H

更新日期：1988-11-30 00:00:00

abstract：:The second open reading frame of avian reovirus S1 gene segment encodes a 17 kDa non-structural protein, named p17. The biological role of p17 is fully unknown so far. Using trypan blue dye exclusion and MTT assay, we demonstrated that the ectopic expression of p17 results in the reduction of viable cell number and ce...

journal_title：Biochemical and biophysical research communications

authors： Liu HJ,Lin PY,Lee JW,Hsu HY,Shih WL

更新日期：2005-10-21 00:00:00

abstract：:It has been well documented that tumor suppressor p53 is mutated in about 50% of all human tumors. p53 status might be one of the critical determinants for the chemo-sensitivity of human tumors. In the present study, we have found that p53 family member p73 as well as 14-3-3sigma is down-regulated in response to adria...

journal_title：Biochemical and biophysical research communications

authors： Sang M,Li Y,Ozaki T,Ono S,Ando K,Yamamoto H,Koda T,Geng C,Nakagawara A

更新日期：2006-08-18 00:00:00

abstract：:Human natural killer enhancing factor genes (NKEF-A and B) share extensive homology with several genes from other organisms known to be induced by oxidative stress and to protect organisms and proteins from oxidative destruction. In this study, we have identified natural killer enhancing factor as an abundant protein ...

journal_title：Biochemical and biophysical research communications

authors： Shau H,Kim A

更新日期：1994-02-28 00:00:00