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Niosomal virosome derived by vesicular stomatitis virus glycoprotein as a new gene carrier.

Abstract：

:Virosomes as membranous vesicles with viral fusion protein in their membrane are versatile vehicles for cargo delivery. The vesicular stomatitis virus glycoprotein (VSV-G) is a common fusogenic protein used in virosome preparation. This glycoprotein has been used in liposomal systems so far, but in this study, we have tried to use the niosomal form instead of liposome for. Niosomes are vesicular systems composed of non-ionic surfactants. Niosomes were constructed by the thin-film hydration method. VSV-G gene in pMD2.G plasmid was expressed in the HEK293T cell line and then was reconstituted in the niosome bilayer. The formation of niosomal virosomes was confirmed with different methods such as SDS-PAGE gel, western blotting, and transmission electron microscopy (TEM). The efficiency of niosomal virosome was investigated with the pmCherry reporter gene. SDS-PAGE and western blotting proved the expression and successful insertion of protein into the bilayer. The TEM images showed the spike projection of VSV-G on the surface of niosomes. The transfection results showed high efficiency of niosomal virosomes as a novel carrier. This report has verified that niosome could be used as an efficient bilayer instead of liposome to construct virosomes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Asadikaram G,Poustforoosh A,Pardakhty A,Torkzadeh-Mahani M,Nematollahi MH

doi

10.1016/j.bbrc.2020.10.054

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

980-987

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(20)31971-9

journal_volume

534

pub_type

杂志文章

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