Recombinant adenovirus infection suppresses hTERT expression through virus-associated RNA-mediated induction of type 1 interferon.

Abstract:

:Adenovirus vector is one of the most widely used vectors in gene therapy applications for the treatment of diverse human diseases including cancer. In this study, we showed that infection with E1E3-deleted recombinant human adenovirus serotype 5 reduced human telomerase reverse transcriptase (hTERT) mRNA levels in hepatoma cell lines. We defined the mechanisms by which the recombinant adenovirus vector reduces hTERT mRNA levels as follows: Using the virus-associated RNA I/II (VAI/II) expression construct, we demonstrated that the expression of VAI and VAII RNAs led to an increase in IFN-α2 level, and IFN-α2 induction was responsible for the decrease in hTERT mRNA levels. We showed that the effects of VA RNAs were specific for the replication-incompetent E1E3-deleted adenovirus vector, because wild-type adenovirus affected neither IFN-α2 nor hTERT mRNA levels. Moreover, we demonstrated that adenovirus vector-mediated delivery of the hTERT-targeting anti-cancer reagent could additively reduce the levels of hTERT mRNA that were specifically overexpressed in most of the cancer cells. This study showed that E1E3-deleted adenovirus vector system reduced hTERT mRNA levels through VA RNA-mediated induction of type 1 interferon; hence the recombinant adenovirus itself could have anti-cancer activity. These results indicate that recombinant adenovirus vector could be an effective means to deliver anti-cancer reagents for combating cancerous cells more effectively.

authors

Lee CH,Kim HW,Kim T,Lee SW

doi

10.1016/j.bbrc.2015.02.039

subject

Has Abstract

pub_date

2015-03-20 00:00:00

pages

830-5

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)00257-0

journal_volume

458

pub_type

杂志文章
  • Binding mode of the oxidized α-anomer of NAD+ to RSP, a Rex-family repressor.

    abstract::The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/α-NAD(+) c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.12.049

    authors: Zheng Y,Ko TP,Yang Y,Shao W,Guo RT

    更新日期:2015-01-16 00:00:00

  • Diacylglycerols enhance human neutrophil degranulation responses: relevancy to a multiple mediator hypothesis of cell function.

    abstract::At 10 microM, 1-0-oleoyl-, 1-0-palmitoyl-, and 1-0-myristoyl-2-0-acetyl-glycerol weakly stimulated neutrophils to release lysozyme, an enzyme in secondary granules, but had no such effect on the release of a primary granule enzyme, beta-glucuronidase. The glycerides (1-10 microM) had a second effect on both granule po...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(84)90380-2

    authors: O'Flaherty JT,Schmitt JD,McCall CE,Wykle RL

    更新日期:1984-08-30 00:00:00

  • The role of wogonin in controlling SOCS3 expression in neuronal cells.

    abstract::The mechanism underlying the wogonin-mediated increase in the expression of suppressor of cytokine signaling 3 (SOCS3) is unclear. Promoter deletion assay results revealed that wogonin-induced SOCS3 expression is dependent on the AP-1 consensus sequences and two STAT responsive elements (TTACAAGAA and TTCCAGGAA) in th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.07.018

    authors: Nam KW,Chae S,Song HY,Mar W,Han MD

    更新日期:2014-08-08 00:00:00

  • Ionophore-induced Cl- transport in human erythrocyte suspensions: a multinuclear magnetic resonance study.

    abstract::To investigate the effect of ionophores on Cl- distribution in human erythrocyte suspensions, we measured the membrane potential by using 19F and 31P NMR methods. Incubation of human erythrocytes with 0.005 mM of the neutral ionophores valinomycin and nonactin resulted in membrane potentials of -21.2 and -17.8 mV in t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)90678-e

    authors: Wittenkeller L,Mota de Freitas D,Ramasamy R

    更新日期:1992-04-30 00:00:00

  • Polyamine levels during Xenopus laevis oogenesis: a role in oocyte competence to meiotic resumption.

    abstract::The results presented here show that a decrease in the concentration of total polyamines, due to a decrease in putrescine and spermine, occurs during oogenesis in Xenopus laevis. The microinjection of spermine or spermidine decreases the hormonal responsiveness (maturation) of the fully-grown oocytes. This effect is s...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(89)80080-4

    authors: Osborne HB,Mulner-Lorillon O,Marot J,Belle R

    更新日期:1989-01-31 00:00:00

  • PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family.

    abstract::Ubiquitinlation of proteins is prevalent and important in both normal and pathological cellular processes. Deubiquitinating enzymes (DUBs) can remove the ubiquitin tags on substrate proteins and dynamically regulate the ubiquitination process. The PPPDE family proteins were predicted to be a novel class of deubiquitin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.04.161

    authors: Xie X,Wang X,Jiang D,Wang J,Fei R,Cong X,Wei L,Wang Y,Chen H

    更新日期:2017-06-24 00:00:00

  • The structure of SecB/OmpA as visualized by electron microscopy: The mature region of the precursor protein binds asymmetrically to SecB.

    abstract::SecB, a molecular chaperone in Escherichia coli, binds a subset of precursor proteins that are exported across the plasma membrane via the Sec pathway. Previous studies showed that SecB bound directly to the mature region rather than to the signal sequence of the precursor protein. To determine the binding pattern of ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.02.062

    authors: Tang Y,Pan X,Tai PC,Sui SF

    更新日期:2010-03-19 00:00:00

  • Disulfide isoforms of recombinant glia maturation factor beta.

    abstract::Recombinant human glia maturation factor beta (r-hGMF-beta) is a single-chain polypeptide (141 amino acid residues) containing three cysteines, at positions 7, 86 and 95. Nascent r-hGMF-beta exists in the reduced state and has no biological activity. The protein can be activated through oxidative refolding by incubati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91209-b

    authors: Zaheer A,Lim R

    更新日期:1990-09-14 00:00:00

  • Histone H1-stimulated phosphorylase phosphatase from rabbit skeletal muscle.

    abstract::A major rabbit skeletal muscle phosphorylase phosphatase activity which is markedly stimulated by histone H1 has been resolved from inhibitor-sensitive phosphorylase phosphatase (type-1 phosphatase), glycogen synthase kinase 3-activated phosphatase, phosphatase heat-stable inhibitor proteins, and alkaline phosphatase ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91228-7

    authors: Mellgren RL,Schlender KK

    更新日期:1983-12-16 00:00:00

  • Markedly decreased binding of vincristine to tubulin in vinca alkaloid-resistant Chinese hamster cells is associated with selective overexpression of alpha and beta tubulin isoforms.

    abstract::Vinca alkaloids are among a number of cytotoxic agents which target tumor cell microtubules. Studies described herein document the basis for one form of acquired resistance to these plant alkaloids involving an alteration of tubulin in a variant (DC-3F/VCRd-5L) of DC-3F Chinese hamster cells. Our results revealed a ma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.2246

    authors: Sirotnak FM,Danenberg KD,Chen J,Fritz F,Danenberg PV

    更新日期:2000-03-05 00:00:00

  • Trim11 increases expression of dopamine beta-hydroxylase gene by interacting with Phox2b.

    abstract::The homeodomain transcription factor Phox2b is one of the key determinants involved in the development of noradrenergic (NA) neurons in both the central nervous system (CNS) and the peripheral nervous system (PNS). Using yeast two-hybrid screening, we isolated a Phox2b interacting protein, Trim11, which belongs to TRI...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.01.165

    authors: Hong SJ,Chae H,Lardaro T,Hong S,Kim KS

    更新日期:2008-04-11 00:00:00

  • Involvement of p53 in alpha4beta1 integrin-mediated resistance of B-CLL cells to fludarabine.

    abstract::We recently showed that alpha4beta1 integrin induces B-cell chronic lymphocytic leukemia (B-CLL) cell resistance to fludarabine-induced apoptosis via upregulation of Bcl-xL. We have now studied whether p53 was involved in this response. Cells from five B-CLL patients with wild-type p53 determined by DNA sequencing, or...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2003.10.054

    authors: de la Fuente MT,Casanova B,Cantero E,Hernández del Cerro M,Garcia-Marco J,Silva A,Garcia-Pardo A

    更新日期:2003-11-21 00:00:00

  • Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts.

    abstract::To explore the mechanism of glucocorticoid-induced osteoporosis, we investigated the effect of glucocorticoid on canonical Wnt signaling that emerged as a novel key pathway for promoting bone formation. Wnt3a increased the T-cell factor (Tcf)/lymphoid enhancer factor (Lef)-dependent transcriptional activity in primary...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.01.117

    authors: Ohnaka K,Tanabe M,Kawate H,Nawata H,Takayanagi R

    更新日期:2005-04-01 00:00:00

  • Deactivation of ROCK-II by Y-27632 enhances basolateral pancreatic enzyme secretion and acute pancreatitis induced by CCK analogues.

    abstract::In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. The ROCK-specific inhibitor, Y-27632, did not alter basal amylase secretion, whereas it potentiated CCK-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)00758-7

    authors: Kusama K,Nozu F,Awai T,Tanaka S,Honma I,Tsunoda Y,Mitamura K

    更新日期:2003-05-30 00:00:00

  • Prostaglandin E1 reduces the keratinocyte toxicity of sorafenib by maintaining signal transducer and activator of transcription 3 (STAT3) activity and enhancing the cAMP response element binding protein (CREB) activity.

    abstract::Hand-foot skin reaction (HFSR) is a common side effect of multiple tyrosine kinase inhibitors (mTKIs). HFSR can necessitate dose reductions or interruption of therapy owing to its negative effect on the quality of life. Therefore, effective use of mTKIs requires measures to prevent HFSR. We evaluated the effect of pro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.02.107

    authors: Shichiri H,Yamamoto K,Tokura M,Ishida T,Uda A,Bito T,Nishigori C,Nakagawa T,Hirano T,Yano I,Hirai M

    更新日期:2017-04-01 00:00:00

  • Vascular smooth muscle cell spreading onto fibrinogen is regulated by calpains and phospholipase C.

    abstract::Fibrinogen deposition and smooth muscle cell migration are important causes of atherosclerosis and angiogenesis. Involvement of calpains in vascular smooth muscle cell adhesion onto fibrinogen was investigated. Using calpain inhibitors, we showed that activation of calpains was required for smooth muscle cell spreadin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5859

    authors: Paulhe F,Bogyo A,Chap H,Perret B,Racaud-Sultan C

    更新日期:2001-11-09 00:00:00

  • Peptide-specific antibodies indicate species heterogeneity of a 42 kDa high-affinity inositol 1,3,4,5-tetrakisphosphate receptor protein from brain.

    abstract::The aim of the present study was to identify a high affinity InsP4 receptor in membranes from cerebellum or brain from several species. In samples obtained from sheep, beef, human and rat, a 42 kDa protein represents an Ins(1,3,4,5)P4 receptor, similar to the InsP4 receptor from pig cerebellum, which we have described...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.2251

    authors: Reiser G,Kunzelmann U,Hülser E,Stricker R,Hoppe J,Lottspeich F,Kalbacher H

    更新日期:1995-09-05 00:00:00

  • Cloning and biochemical characterization of a novel mouse ADP-dependent glucokinase.

    abstract::Glycolysis, the catabolism of glucose to pyruvate, is an iconic central metabolic pathway and often used as a paradigm for explaining the general principles of the regulation/control of cellular metabolism. The ubiquitous mammalian ATP-dependent hexokinases I-III and hexokinase IV, also termed glucokinase, initiate th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.01.103

    authors: Ronimus RS,Morgan HW

    更新日期:2004-03-12 00:00:00

  • Heparin increases mRNA levels of thrombospondin but not fibronectin in human vascular smooth muscle cells.

    abstract::The effects of heparin (180 micrograms/ml) on steady state mRNA levels for fibronectin, thrombospondin, actin and collagen types I and III were investigated in human umbilical artery smooth muscle cells. Heparin caused a 120% increase in thrombospondin mRNA levels and a 60% and 180% increase in the mRNA levels of proc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)90786-9

    authors: Lyons-Giordano B,Brinker JM,Kefalides NA

    更新日期:1989-08-15 00:00:00

  • CREB-mediated Bcl-2 expression in trichosanthin-induced Hela cell apoptosis.

    abstract::Bcl-2 plays a pivotal role in the control of cell death and is down-regulated in trichosanthin (TCS)-induced cell apoptosis. Because Bcl-2 expression is regulated by the transcription factor cyclic AMP response element-binding protein (CREB), we investigated the role of CREB activation in TCS-induced Hela cells apopto...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.08.141

    authors: Wang P,Yan H,Li JC

    更新日期:2007-11-09 00:00:00

  • Intronic microRNAs.

    abstract::MicroRNAs (miRNAs), small single-stranded regulatory RNAs capable of interfering with intracellular mRNAs that contain partial complementarity, are useful for the design of new therapies against cancer polymorphism and viral mutation. MiRNA was originally discovered in the intergenic regions of the Caenorhabditis eleg...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.10.215

    authors: Ying SY,Lin SL

    更新日期:2005-01-21 00:00:00

  • Genome-to-function characterization of novel fungal P450 monooxygenases oxidizing polycyclic aromatic hydrocarbons (PAHs).

    abstract::Fungi, particularly the white rot basidiomycetes, have an extraordinary capability to degrade and/or mineralize (to CO(2)) the recalcitrant fused-ring high molecular weight (4 aromatic-rings) polycyclic aromatic hydrocarbons (HMW PAHs). Despite over 30years of research demonstrating involvement of P450 monooxygenation...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.07.094

    authors: Syed K,Doddapaneni H,Subramanian V,Lam YW,Yadav JS

    更新日期:2010-09-03 00:00:00

  • Proprotein convertases regulate insulin-like growth factor 1-induced membrane-type 1 matrix metalloproteinase in VSMCs via endoproteolytic activation of the insulin-like growth factor-1 receptor.

    abstract::The IGF-1 receptor (IGF-1R) and MT1-MMP are synthesized as larger precursor proproteins, which require endoproteolytic activation by the proprotein convertases (PCs) furin/PC5 to gain full biological activity. The aim of this study was to investigate the contribution of PCs to IGF-1R and/or MT1-MMP activation in vascu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.07.001

    authors: Stawowy P,Kallisch H,Kilimnik A,Margeta C,Seidah NG,Chrétien M,Fleck E,Graf K

    更新日期:2004-08-27 00:00:00

  • Effects of hydroxyl radical and sulfhydryl reagents on the open probability of the purified cardiac ryanodine receptor channel incorporated into planar lipid bilayers.

    abstract::We examine the effect of hydroxyl radical on the ion permeability of the ryanodine receptor, a calcium releasing channel of sarcoplasmic reticulum. The cardiac ryanodine receptor, purified from pig heart, was reconstituted to proteoliposomes and then incorporated into a planar bilayer membrane. A single channel activi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.9244

    authors: Anzai K,Ogawa K,Kuniyasu A,Ozawa T,Yamamoto H,Nakayama H

    更新日期:1998-08-28 00:00:00

  • Three-hybrid strategy reveals a peptide segment that specifically binds to the 3'-untranslated region of NF-IL6 mRNA.

    abstract::The 3'UTR of eukaryotic mRNA is an important regulation region, on which many trans factors act. In recent years, a series of 3'UTRs were shown to have tumor suppressor function, including the 3'UTR of the human nuclear factor for interleukin-6 (NF-IL6 3'UTR). To understand molecular basis for this function, we have t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3425

    authors: He YZ,Liu DG

    更新日期:2000-09-16 00:00:00

  • The synthesis, bioactivity and enzyme stability of D-Ala2, EPhe4, Leu5-enkephalins.

    abstract::We have synthesized the first enkephalin analog containing a "cyclopropyl" phenylalanine (Phe) residue. The E-configuration of this residue is apparently responsible for its low activity in the MVD and GPI muscle assays. The enkephalin is very stable to cleavage by carboxypeptidase Y. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)90976-2

    authors: Kimura H,Stammer CH,Shimohigashi Y,Ren-Lin C,Stewart J

    更新日期:1983-08-30 00:00:00

  • Computer studies on the stereostructure and quantum chemical properties of 6-pyruvoyl tetrahydropterin, the key intermediate of tetrahydrobiopterin biosynthesis.

    abstract::The optimized geometry of the conformation of atoms constituting the 6-pyruvoyl tetrahydropterin molecule, the labile key intermediate of tetrahydrobiopterin biosynthesis, was obtained by molecular orbital calculations within the MINDO/3 framework. The stereostructure of the molecule showing the preferred mode for bin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)90888-e

    authors: Katoh S,Sueoka T,Kurihara T

    更新日期:1991-04-15 00:00:00

  • Regulation of hepatic branched-chain alpha-keto acid dehydrogenase kinase in a rat model for type 2 diabetes mellitus at different stages of the disease.

    abstract::Branched-chain alpha-keto acid dehydrogenase (BCKDH) kinase (BDK) is responsible for the regulation of BCKDH complex, which is the rate-limiting enzyme in the catabolism of branched-chain amino acids (BCAAs). In the present study, we investigated the expression and activity of hepatic BDK in spontaneous type 2 diabete...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.02.004

    authors: Doisaki M,Katano Y,Nakano I,Hirooka Y,Itoh A,Ishigami M,Hayashi K,Goto H,Fujita Y,Kadota Y,Kitaura Y,Bajotto G,Kazama S,Tamura T,Tamura N,Feng GG,Ishikawa N,Shimomura Y

    更新日期:2010-03-05 00:00:00

  • Dynamic association of topoisomerase II to the mitotic chromosomes in live cells of Aspergillus nidulans.

    abstract::DNA topoisomerase II (Topo II) is an essential enzyme that catalyzes topological changes of DNA and consists of a major member of mitotic chromosomes. To investigate the dynamic localization of Topo II in nuclei, we engineered the strain of Aspergillus nidulans expressing Topo II fused with green fluorescent protein (...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.06.135

    authors: Kawagishi M,Akashi T,Kikuchi A

    更新日期:2005-08-26 00:00:00

  • Sequence homology between the coat proteins of DNA and RNA plant viruses.

    abstract::The coat proteins of the leafhopper transmitted gemini viruses, viz., maize streak virus and wheat dwarf virus that contain a single circle of DNA in their genomes are shown to bear good amino acid sequence homology with the coat protein of an RNA plant virus, the satellite tobacco necrosis virus. It is suggested that...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90573-5

    authors: Mohana Rao JK

    更新日期:1986-01-14 00:00:00