Abstract:
:Topical application of hexadecane has been shown to induce hyperproliferation and hyperkeratosis in rodent skin. The application of hexadecane to epidermis from the backs of piglets less than 1 week old resulted in a rapid biphasic-rise in the level of ornithine decarboxylase (ODC) activity. The second phase of the elevation of activity was suppressed by cycloheximide indicating that it resulted from de novo protein synthesis. The first, cycloheximide-insensitive phase presumably represents activation of existing enzyme. The activation of this latent ODC by hexadecane was independent of extracellular calcium. A similar degree of activation was observed using the bivalent-cation ionophore A23187 which augmented the hexadecane effect implicating a rise in intracellular calcium concentration as a possible cause for the activation possibly via the receptor-mediated phospholipid hydrolysis. The time-course of the ODC activation also corresponded with a rapid fall in cAMP levels indicating a possible role for cAMP in ODC regulation.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Owusu S,Cunliffe WJ,Wood EJdoi
10.1016/0006-291x(89)91383-1subject
Has Abstractpub_date
1989-06-30 00:00:00pages
1291-8issue
3eissn
0006-291Xissn
1090-2104pii
0006-291X(89)91383-1journal_volume
161pub_type
杂志文章abstract::Parathyroid hormone-related protein (PTHrP) is produced by various normal and neoplastic tissues. Even if the physiological function(s) of PTHrP is unclear, evidence suggests that the protein may participate in the local regulation of smooth muscle contractility. We show here that PTHrP is produced in endothelial cell...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1994.1107
更新日期:1994-01-28 00:00:00
abstract::In the early postnatal period, cerebellar granule cells exhibit an activity-dependent downregulation of a set of immaturation genes involved in cell growth and migration and are shifted to establishment of a mature network formation. Through the use of a granule cell culture and both pharmacological and RNA interferen...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2016.04.017
更新日期:2016-05-13 00:00:00
abstract::Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 A. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. Thes...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/S0006-291X(02)00555-7
更新日期:2002-06-21 00:00:00
abstract::To elucidate the role of endogenous endothelin on DNA synthesis in endothelial cells, we have investigated the effects of rabbit anti-ET gamma globulin on DNA synthesis in cultured human vascular endothelial cells. DNA synthesis was markedly inhibited by anti-ET gamma globulin in a concentration dependent manner in th...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(90)92354-3
更新日期:1990-04-30 00:00:00
abstract::Neurogenin-3 (ngn-3) expression is critical for endocrine development in the developing pancreas. We found that when ngn-3 was inhibited in an E11.5 pancreas, using either morpholino antisense or siRNA, it led to a significant decrease in endocrine differentiation after seven days in culture. Endocrine differentiation...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2010.05.058
更新日期:2010-06-11 00:00:00
abstract::Pancreatic islets of Langerhans were perifused with Krebs-bicarbonate solution containing glucose (5 and 10 mM). The perifusate was spiked with tetradeuterated hepoxilin A3 and was extracted and analysed by gas chromatography-mass spectrometry using NICI detection. Evidence is presented showing the presence of hepoxil...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(85)90137-8
更新日期:1985-04-30 00:00:00
abstract::The present study was carried out to investigate the effects of Interleukin-1 (IL-1) on gastric pepsin secretion in conscious pylorus-ligated rats. The intraperitoneal (ip) injection of IL-1 resulted in a dose-related inhibition of gastric pepsin output. The intracerebroventricular (icv) injection of IL-1 similarly re...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(90)90616-u
更新日期:1990-03-30 00:00:00
abstract::As an important factor leading to aging and chronic diseases, oxidative stress has become a hot research topic. Trehalose is a natural sugar widely found in many edible plants, animals and natural microorganisms, and recent studies have suggested that trehalose is an antioxidant, although its underlying molecular mech...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2019.10.088
更新日期:2020-01-01 00:00:00
abstract::We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2007.05.201
更新日期:2007-08-03 00:00:00
abstract::High affinity interactions between cells and laminin are mediated, at least in part, by the 67 kDa laminin receptor (67 LR). A 37 kDa nascent polypeptide (37 LRP), predicted by a full length cDNA clone and obtained by in vitro translation of hybrid-selected laminin receptor mRNA, has been immunologically identified in...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(91)91965-f
更新日期:1991-05-31 00:00:00
abstract::Recombinant human glia maturation factor beta (r-hGMF-beta) is a single-chain polypeptide (141 amino acid residues) containing three cysteines, at positions 7, 86 and 95. Nascent r-hGMF-beta exists in the reduced state and has no biological activity. The protein can be activated through oxidative refolding by incubati...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(90)91209-b
更新日期:1990-09-14 00:00:00
abstract::The metalated forms of metallothionein are well studied (particularly Zn-MT, Cu-MT and Cd-MT), but almost nothing is known about the chemical and structural properties of apometallothioneins despite their importance in initial metalation and subsequent demetalation. Electrospray ionization mass spectrometry was used t...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2012.07.141
更新日期:2012-08-24 00:00:00
abstract::Phage displayed peptide library was used to select tumor necrosis factor alpha (TNFalpha) binding peptides. After three sequential rounds of biopanning, some linear TNFalpha-binding peptides were identified from a 12-mer peptide library. A consensus sequence (L/M)HEL(Y/F)(L/M)X(W/Y/F), where X might be variable residu...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2003.09.141
更新日期:2003-10-31 00:00:00
abstract::Transcription factor GATA-6 plays essential roles in developmental processes and tissue specific functions through regulation of gene expression. GATA-6 mRNA utilizes two Met-codons in frame as translational initiation codons. Deletion of the nucleotide sequence encoding the PEST sequence (Glu(31)-Cys(46)) between the...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2014.09.019
更新日期:2014-10-03 00:00:00
abstract::Helicobacter pylori is a potent carcinogen associated with gastric cancer malignancy. Recently, H. pylori Heat shock protein 60 (HpHSP60) has been reported to promote cancer development by inducing chronic inflammation and promoting tumor cell migration. This study demonstrates a role for HpHSP60 in angiogenesis, a ne...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2010.05.101
更新日期:2010-06-25 00:00:00
abstract::The acute phase of an inflammation induced in young rats, at various ages between 1 and 21 days after birth, by a single subcutaneous turpentine injection, is accompanied by a 35%-45% decrease of the alpha 1-foetoprotein serum concentrations. This fall is demonstrated with equilibrium dialysis binding methods, evidenc...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(83)91032-x
更新日期:1983-02-10 00:00:00
abstract::GLAST and GLT-1 are the most abundant glutamate transporters in the CNS and protect neurons from glutamate neurotoxicity. Here, we investigated the role of GLAST in spinal nociceptive processing. GLAST protein expression was not altered after treatment of rats with either formalin or zymosan. Surprisingly, knock-down ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2006.05.163
更新日期:2006-07-28 00:00:00
abstract::Two genomic clones containing the 3'-end of CYP3A7, the whole 5'-flanking region plus the first exon of CYP3A5 were isolated from two different human genomic banks and sequenced. The 5'-flanking region of CYP3A5, from nucleotide -1 to -1434, was 60% and 59% similar with the corresponding regions of CYP3A4 and CYP3A7, ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1994.2870
更新日期:1994-12-30 00:00:00
abstract::Transgenic mice were produced by the microinjection of the cDNA of human alpha-fetoprotein (AFP) under control of the enhancer/promoter of the human beta-actin gene. Among the 4 mouse lines where the transgene were stable transmitted to the progeny, 3 produced human AFP. The expression was not developmental stage-spec...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1993.1276
更新日期:1993-03-15 00:00:00
abstract::Evidence of a complex formation is a crucial step in the structural studies of ligand-receptor interactions. Here we presented a simple and fast approach for qualitative screening of the complex formation between the chimeric extracellular domain of the nicotinic acetylcholine receptor (α7-ECD) and three-finger protei...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2020.08.021
更新日期:2020-10-29 00:00:00
abstract::We cloned a cDNA encoding a novel extracellular signal-regulated kinase (ERK) 2-binding protein, EBITEIN1, by yeast two-hybrid screening. Northern and Western blotting experiments showed that the transcript and protein were expressed in the testes. Furthermore, immunohistochemical experiments showed that EBITEIN1 exis...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2008.01.078
更新日期:2008-04-04 00:00:00
abstract::The function and intracellular localisation of the non-catalytic NH(2)-terminal region of focal adhesion kinase (FAK) are unclear. We investigated the targetting of the FAK NH(2)-terminal domain in HEK 293 and epithelial MDCK cells. Exogenous expression of a variety of GFP-fused and epitope-tagged NH(2) terminal domai...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/s0006-291x(02)02547-0
更新日期:2002-11-22 00:00:00
abstract::Chemiluminescence in visible region was detected, during peroxidase-catalyzed oxidation of chlorpromazine at pH7.5 (but not at pH4.25) or of propericiazine, both at pH7.5 and at pH4.25. Red colored intermediates, cation radicals, were produced and decayed in all enzymatic systems used. Chemiluminescence was also detec...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(85)91707-3
更新日期:1985-08-15 00:00:00
abstract::Ankylosing spondylitis (AS) is a chronic systemic inflammatory disorder of the axial skeleton and shows significant inherited susceptibility. Auto-immune responses have been traditionally considered as a putative pathogenetic event in AS. However, no consistent self-antigen has been identified to responsible for the d...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2007.03.179
更新日期:2007-06-01 00:00:00
abstract::Apoptosis is a well defined physiological process characterised by many specific features including DNA fragmentation and protease activation. Cell membrane-associated changes such as the altered exposure of phosphatidylserine and glycosylation patterns have also been described. We investigate here the change in expos...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1998.9337
更新日期:1998-09-18 00:00:00
abstract::A more precise description of the binding of N-(phosphonacetyl)-L- aspartate to the catalytic chains of aspartate carbamoyltransferase clarifies aspects of the specificity of this enzyme toward its substrates, carbamoylphosphate and L-aspartate, and suggests a catalytic role for His-134. ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(86)90514-0
更新日期:1986-04-29 00:00:00
abstract::The c-myc oncogene is implicated in normal growth and differentiation processes. Human cell lines IM9 and HepG2 stably cultured at "low" glucose concentrations (5.5 mM) show c-myc mRNA levels 3-4 times higher than cells cultured at "high" glucose concentrations (25 nM). D-fructose (a metabolizable exose) substitutes f...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(89)92719-8
更新日期:1989-12-29 00:00:00
abstract::Cisplatin is one of the most widely used anticancer agents. Cisplatin-induced cytotoxicity results from its ability to form cisplatin-DNA adducts within the cellular genome which can inhibit the transcription of genes and the replication of DNA. Cisplatin-adducts are primarily removed by the nucleotide excision repair...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1998.9366
更新日期:1998-09-29 00:00:00
abstract::Low temperatures cause cellular damage in flower buds of the sweet cherry (Prunus avium L. cv. Satohnishiki). In this study, the redox states within the cherry buds suffering freezing damage were non-destructively observed by a unique in vivo electron spin resonance (ESR) technique with a spin probe such as carbamoyl-...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2003.08.123
更新日期:2003-10-10 00:00:00
abstract::Release of soluble growth hormone binding protein (GHBP) corresponding to the extracellular domain of the GH receptor (GHR) occurs via distinct mechanisms depending on species. In human, proteolysis of full length GHR results in liberation of GHBP into the extracellular medium. A putative protease responsive for GHR c...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.2001.6261
更新日期:2002-01-18 00:00:00