Tyrosine residues of the erythropoietin receptor are dispensable for erythroid differentiation of human CD34+ progenitors.

abstract：:We have used HL-60 leukemia cells to investigate phosphatidylinositol 3-kinase (PI 3-K) during granulocytic differentiation at the nuclear level. Nuclei of HL-60 cells showed a constitutive presence of PI 3-K that increased when cells were treated with differentiating doses of ATRA. PI 3-K was also detected tightly bo...

journal_title：Biochemical and biophysical research communications

authors： Marchisio M,Bertagnolo V,Colamussi ML,Capitani S,Neri LM

更新日期：1998-12-18 00:00:00

abstract：:We investigated the endogenous mono(ADP-ribosyl)ation of the sarcoplasmic reticulum from rabbit skeletal muscle. The autoradiogram obtained after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the [adenylate-32P]NAD-treated sarcoplasmic reticulum vesicles revealed a major band corresponding to the MW 105...

journal_title：Biochemical and biophysical research communications

authors： Hara N,Mishima K,Tsuchiya M,Tanigawa Y,Shimoyama M

更新日期：1987-04-29 00:00:00

abstract：:In the mouse fibroblasts BP-A31 as well as in the human epidermoid carcinoma cells KB-3-1, both cyclin D1 mRNA and protein contents decreased rapidly during incubation with sodium butyrate. The decrease of cyclin D1 mRNA was not prevented by cycloheximide indicating that protein synthesis is not required for the inhib...

journal_title：Biochemical and biophysical research communications

authors： Lallemand F,Courilleau D,Sabbah M,Redeuilh G,Mester J

更新日期：1996-12-04 00:00:00

abstract：:A novel neurotensin-like peptide, designated neuromedin N, has been isolated from porcine spinal cord by using a bioassay for a stimulant effect on guinea pig ileum. By microsequencing, the amino acid sequence of the peptide has been determined to be Lys-Ile-Pro-Tyr-Ile-Leu, which is found to be quite homologous to th...

journal_title：Biochemical and biophysical research communications

authors： Minamino N,Kangawa K,Matsuo H

更新日期：1984-07-31 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARgamma), a member of nuclear receptors, is expressed at a high level in adipose tissue and plays an important role in adipocyte differentiation. In the present study, we identified the expression of PPARgamma in rat aortic smooth muscle cells (RASMC) using reverse t...

journal_title：Biochemical and biophysical research communications

authors： Iijima K,Yoshizumi M,Ako J,Eto M,Kim S,Hashimoto M,Sugimoto N,Liang YQ,Sudoh N,Toba K,Ouchi Y

更新日期：1998-06-18 00:00:00

abstract：:A protein extract prepared from the egg shell fiber-secreting region of the oviduct of Alligator mississippiensis was assayed for the presence of receptors for estrogen and progesterone. In the presence of [3H]-estradiol-17 beta, the extract contained an estrogen-binding activity which reached equilibrium at 25 degree...

journal_title：Biochemical and biophysical research communications

authors： Vonier PM,Guillette LJ Jr,McLachlan JA,Arnold SF

更新日期：1997-03-17 00:00:00

abstract：:The high affinity laminin receptor is differentially expressed in metastasis. We now report that there are multiple copies (6 +/- 1) of the laminin receptor gene in the murine genome of normal diploid cells as well as in cell lines derived from cancer cells. We have analyzed three distinct cDNA clones isolated from an...

journal_title：Biochemical and biophysical research communications

authors： Fernández MT,Castronovo V,Rao CN,Sobel ME

更新日期：1991-02-28 00:00:00

abstract：:A cDNA for tetrameric carbonyl reductase was cloned from a pig lung cDNA library. The cDNA had an open reading frame encoding a protein of 244 amino acid residues with a predicted monomer molecular weight of 25,985. The authenticity of the cDNA was confirmed by a perfect match of 80 amino acids determined from sequenc...

journal_title：Biochemical and biophysical research communications

authors： Nakanishi M,Deyashiki Y,Nakayama T,Sato K,Hara A

更新日期：1993-08-16 00:00:00

abstract：:Cholesterol is a critical component of lipid rafts implicated in regulating multiple signal transduction. The anthrax toxin receptor 2 (ANTXR2) is a type I membrane protein acting as the second receptor for the anthrax toxin. In this study, we first investigated the association between cholesterol and ANTXR2. We provi...

journal_title：Biochemical and biophysical research communications

authors： Zou J,Xu L,Ju Y,Zhang P,Wang Y,Zhang B

更新日期：2014-09-12 00:00:00

abstract：:Intravenous immunoglobulin (IVIG) is periodically administered to immunocompromised patients together with antimicrobial agents. The evidence that supports the effectiveness of IVIG is mostly based on data from randomized clinical trials; the underlying mechanisms are poorly understood. A recent study revealed that ki...

journal_title：Biochemical and biophysical research communications

authors： Matsuo H,Itoh H,Kitamura N,Kamikubo Y,Higuchi T,Shiga S,Ichiyama S,Kondo T,Takaori-Kondo A,Adachi S

更新日期：2015-08-14 00:00:00

abstract：:In our previous study, the novel GRN-41 peptide generated from alternative splicing of the Mozambique tilapia PGRN1 gene was identified to be a potent peptide that protected against V. vulnificus in the transgenic zebrafish model by modulating innate immune-related genes. In this study, the anti-bacterial activities o...

journal_title：Biochemical and biophysical research communications

authors： Wu SH,Chou HY,Liu PC,Wu JL,Gong HY

更新日期：2019-08-06 00:00:00

abstract：:Histamine induced a concentration-dependent increase in intracellular cyclic-AMP of the two human melanoma cell lines SK23 and DX3.LT5.1; maximal stimulation was obtained with 17.8 microM histamine which consistently produced greater than 50-fold increases in the cyclic AMP content of both cell lines. The dose-respons...

journal_title：Biochemical and biophysical research communications

authors： Whitehead RJ,Taylor DJ,Evanson JM,Hart IR,Woolley DE

更新日期：1988-02-29 00:00:00

abstract：:Calcium/calmodulin-dependent protein kinase II (CaM KII) is thought to be involved in the majority of the neuronal functions mediated by intracellular free Ca(2+), and has been implicated in long-term potentiation, learning, and memory. In this work, we have examined in detail the RNA expression pattern for the Drosop...

journal_title：Biochemical and biophysical research communications

authors： Rachidi M,Lopes C,Takamatsu Y,Ohsako S,Benichou JC,Delabar JM

更新日期：1999-07-14 00:00:00

abstract：:Cofilin, a key regulator of actin filament dynamics, binds to G- and F-actin and promotes actin filament turnover by stimulating depolymerization and severance of actin filaments. In this study, cytochalasin D (CytoD), a widely used inhibitor of actin dynamics, was found to act as an inhibitor of the G-actin-cofilin i...

journal_title：Biochemical and biophysical research communications

authors： Shoji K,Ohashi K,Sampei K,Oikawa M,Mizuno K

更新日期：2012-07-20 00:00:00

abstract：:7,8-Dihydrobiopterin is not an intermediate in the de novo biosynthesis of tetrahydrobiopterin, the cofactor required for aromatic amino acid hydroxylations. However, N-acetyl-serotonin inhibition of sepiapterin reductase, an enzyme whose previously only known function was the reduction of sepiapterin to 7,8-dihydrobi...

journal_title：Biochemical and biophysical research communications

authors： Milstien S,Kaufman S

更新日期：1983-09-30 00:00:00

abstract：:Casper/c-FLIP is a caspase-8-related molecule critically involved in regulation of death receptor-induced apoptosis. It has been shown that Casper can either promote or antagonize apoptosis and can activate the transcription factor NF-kappaB. The exact functions of Casper are controversial. To further understand how C...

journal_title：Biochemical and biophysical research communications

authors： Li Z,Zhang J,Chen D,Shu HB

更新日期：2003-10-03 00:00:00

abstract：:Glial precursor cells (GPCs) are present in the adult human central nervous system (CNS) and they can be isolated and maintained in culture for in vitro studies. This study analysed expression of mGluR3 and mGluR5 metabotropic glutamate receptor (mGluR) mRNAs in GPCs. A2B5 surface antigen positive GPCs were isolated u...

journal_title：Biochemical and biophysical research communications

authors： Luyt K,Varadi A,Halfpenny CA,Scolding NJ,Molnar E

更新日期：2004-06-18 00:00:00

abstract：:Mutation of the Patched gene has been detected in human inherited basal cell nevus syndrome (BCNS) and sporadic basal cell carcinomas (BCC), suggesting a strong relation between a Sonic hedgehog-Patched signal and cell proliferation. In the present study, we demonstrate that Sonic hedgehog is expressed in human lung s...

journal_title：Biochemical and biophysical research communications

authors： Fujita E,Khoroku Y,Urase K,Tsukahara T,Momoi MY,Kumagai H,Takemura T,Kuroki T,Momoi T

更新日期：1997-09-18 00:00:00

abstract：:Sinusoidal endothelial cells proliferate following hepatocyte regeneration in the liver after partial resection. The expressions of vascular endothelial growth factor (VEGF) and its receptors, flt-1 and KDR/flk-1, were studied by Northern blotting in isolated rat liver cells and 70% resected rat liver. VEGF was expres...

journal_title：Biochemical and biophysical research communications

authors： Mochida S,Ishikawa K,Inao M,Shibuya M,Fujiwara K

更新日期：1996-09-04 00:00:00

abstract：:Interleukin-1 alpha (IL-1alpha) regulates a wide range of important cellular processes. In this study for the first time, we report the cloning, expression, biophysical, and biological characterization of the human interleukin-1alpha. Human IL-1alpha has been expressed in Escherichia coli in high yields ( approximatel...

journal_title：Biochemical and biophysical research communications

authors： Rajalingam D,Kacer D,Prudovsky I,Kumar TK

更新日期：2007-08-31 00:00:00

abstract：:Hexokinase 1 (HK1) purified from rat brain exhibits protein kinase activity, including autophosphorylation and phosphorylation of histone H2A. This protein kinase activity is observed only in the absence of the HK1 carbohydrate substrate, glucose. Analysis of the ATP-binding domains of the mammalian HK1 protein sequen...

journal_title：Biochemical and biophysical research communications

authors： Adams V,Griffin LD,Gelb BD,McCabe ER

更新日期：1991-06-28 00:00:00

abstract：:Using a highly sensitive RT-PCR technique that measures mRNA (cDNA)-to-DNA ratios, we are able to detect constitutive CYP1A1 mRNA in adult mouse liver as well as in the oocyte. Twelve hours after fertilization of the ovum, there is a more than 100-fold increase in constitutive CYP1A1 mRNA levels; this dramatic increas...

journal_title：Biochemical and biophysical research communications

authors： Dey A,Nebert DW

更新日期：1998-10-20 00:00:00

abstract：:Kinetic analyses were performed to understand the mechanism of hyperoxic induced inhibition of prostacyclin synthesis by human umbilical arteries. Brief exposure of arterial segments to oxygen resulted in over 30% decrease in Vmax of cyclooxygenase in treated vessels. In contrast, cyclooxygenase from hypoxic arterial ...

journal_title：Biochemical and biophysical research communications

authors： Yamaja Setty BN,Walenga RW,Stuart MJ

更新日期：1984-11-30 00:00:00

abstract：:We have designed and constructed a plasmid (pE7RZ-1) that contains a gene for a hammerhead ribozyme that specifically cleaves cottontail rabbit papillomavirus (CRPV) E7 RNA sequences in vitro. The in vitro produced transcripts of pE7RZ-1 cleave CRPV E7 target sequences in trans at 37 degrees C. Pretreatment of pE7RZ-1...

journal_title：Biochemical and biophysical research communications

authors： Wisotzkey JD,Krizenoskas A,DiAngelo S,Kreider JW

更新日期：1993-04-30 00:00:00

abstract：:The chemokine receptor CCR-7 is expressed in T, NK, and dendritic cells in a time-ordered and stimulus-dependent manner. Thorough analyses of the pharmacological profiles of the recombinant ligands for CCR-7, MIP-3beta/ELC/CK-beta 11, and SLC/Exodus-2/TCA4/6C-kine, using CCR-7-expressing HEK-293E transfectants determi...

journal_title：Biochemical and biophysical research communications

authors： Sullivan SK,McGrath DA,Grigoriadis D,Bacon KB

更新日期：1999-10-05 00:00:00

abstract：:Nischarin, a novel integrin binding protein, has been demonstrated its negative effects on cell migration and invasion. However, the biological role of Nischarin in breast cancer has not been fully elucidated yet. Our study aimed to analyze the association between Nischarin expression and clinical features of breast c...

journal_title：Biochemical and biophysical research communications

authors： Chang C,Wei W,Han D,Meng J,Zhu F,Xiao Y,Wu G,Shi X,Zhang L

更新日期：2017-03-11 00:00:00

abstract：:Long-term neuropathic pain can lead to anxiety, depression, and other issues, which seriously affect patients' quality of life. For this reason, it is important to find effective treatments. Studies have shown that glial cell-derived neurotrophic factor (GDNF) can relieve neuropathic pain. However, its mechanism of ac...

journal_title：Biochemical and biophysical research communications

authors： Pan Y,Jiang L,Wang C

更新日期：2020-02-05 00:00:00

abstract：:Plant sterols are supplemented in foods to reduce cardiovascular risk. Randomized controlled trials show 2 g of plant sterols a day reduce serum cholesterol by about 10%. This reduction in serum cholesterol levels is achieved at the expense of increased serum plant sterol levels. Findings in patients with phytosterole...

journal_title：Biochemical and biophysical research communications

authors： Weingärtner O,Baber R,Teupser D

更新日期：2014-04-11 00:00:00

abstract：:The present study was conducted to determine whether fetuin-A, a dominant serum protein plays a role in chemo-attraction and chemo-invasion of carcinoma cells in vitro. Serum is normally used as positive chemotaxis control in Boyden chamber motility assays, prompting the need to identify the factor/s in serum that con...

journal_title：Biochemical and biophysical research communications

authors： Nangami GN,Watson K,Parker-Johnson K,Okereke KO,Sakwe A,Thompson P,Frimpong N,Ochieng J

更新日期：2013-09-06 00:00:00

abstract：:The chaperonin from the acidothermophilic archaeon, Sulfolobus sp. Strain 7, is composed of two kinds of subunits designated as Scp alpha and Scp beta. In this study, we characterized the recombinant Scp alpha and Scp beta, which were separately expressed in Escherichia coli. Both of them were able to assemble to homo...

journal_title：Biochemical and biophysical research communications

authors： Yoshida T,Yohda M,Suzuki M,Yazaki K,Miura K,Endo I

更新日期：1998-01-26 00:00:00