Antibodies elicited by the primary response are useful as ligands for immunoaffinity chromatography.


:A suitable antibody for immunoaffinity chromatography is considered to efficiently bind the antigen in the adsorption step and to dissociate the bound antigen with mild changes in the environmental conditions. To develop an effective method for preparing such antibodies, murine antisera elicited by the primary and secondary responses against bovine beta-lactoglobulin, a model protein antigen, were examined and compared for their ability to bind the antigen under differing pH and ionic strength conditions. Antibodies from the primary response were usually found to be highly sensitive to changes in pH and ionic strength, suggesting that these antibodies may be useful as ligands for immunoaffinity chromatography.


Enomoto A,Kamata N,Nakamura K




Has Abstract


1994-06-15 00:00:00














  • Human cartilage from late stage familial osteoarthritis transcribes type II collagen mRNA encoding a cysteine in position 519.

    abstract::A single base change resulting in the substitution of Cys for Arg at position 519 of the type II collagen triple helix is a predisposing factor in the pathogenesis of a precocious-onset form of familial osteoarthritis associated with a mild chondrodysplasia. Cartilage obtained at the time of total knee replacement in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Holderbaum D,Malemud CJ,Moskowitz RW,Haqqi TM

    更新日期:1993-05-14 00:00:00

  • Homozygous deletions of the MTS1 gene are rare in non-astrocytic brain tumors.

    abstract::Several studies support a role for the multiple tumor suppressor gene (MTS1) in the malignant progression of different tumor types. In this study we have examined the status of the MTS1 gene in a variety of non-astrocytic tumors of the central nervous system. It was not possible, using multiplex PCR with primers for M...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Walker DG,Duan W,Kaye AH,Lavin MF

    更新日期:1995-06-15 00:00:00

  • Biological effects of anti-ErbB2 single chain antibodies selected for internalizing function.

    abstract::Two internalizing monovalent single chain antibody fragments (scFv), C6.5 and F5, that recognize distinct ErbB2 extracellular domain (ECD) epitopes, and their bivalent forms dbC6.5 and F5(scFv')(2), were compared to the growth-inhibiting anti-ErbB2 antibody Herceptin/trastuzumab, in either its bivalent (Her) or monova...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Neve RM,Nielsen UB,Kirpotin DB,Poul MA,Marks JD,Benz CC

    更新日期:2001-01-12 00:00:00

  • Distinct role of the intracellular C-terminus for subcellular expression, shedding and function of the murine transmembrane chemokine CX3CL1.

    abstract::The transmembrane chemokine CX3CL1 is expressed on the endothelial surface and promotes leukocyte adhesion and transmigration by receptor interaction via its extracellular chemokine domain. Since little is known about its intracellular C-terminus, we examined the consequences of C-terminal truncation on cellular distr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Andrzejewski MG,Koelsch A,Kogel T,Dreymueller D,Schwarz N,Ludwig A

    更新日期:2010-04-30 00:00:00

  • Relative spectral response as a function of sequential ligand binding.

    abstract::A unique method is presented for the determination of the critical number of ligands that must bind to a macromolecule to elicit a spectroscopic response. This method is based on analysis of ligand binding data. For example, four Ca2+ and two Mg2+ ions are necessary for mirroring the relative decrease in the intrinsic...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Deerfield DW 2nd,Hoke RA,Pedersen LG,Darden T,Hiskey RG

    更新日期:1986-12-30 00:00:00

  • Aldosterone promotes fibronectin production through a Smad2-dependent TGF-beta1 pathway in mesangial cells.

    abstract::Accumulating evidence demonstrates that aldosterone can cause extra-cellular matrix (ECM) accumulation, in addition to regulating sodium and potassium homeostasis. Increased extra-cellular matrix production by renal glomerular mesangial cells has been suggested to be involved in pathogenesis of glomerular sclerosis. T...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lai L,Chen J,Hao CM,Lin S,Gu Y

    更新日期:2006-09-15 00:00:00

  • An improved method for exchanging troponin subunits in detergent skinned rat cardiac fiber bundles.

    abstract::We describe a method for the removal of endogenous troponin (Tn) complex from bundles of detergent-treated cardiac fibers. After 70 min treatment with cTnT-cTnI most of the endogenous Tn complex was removed from fiber bundles. Complete reconstitution of the Tn complex was achieved by reconstituting with cardiac tropon...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chandra M,Kim JJ,Solaro RJ

    更新日期:1999-09-16 00:00:00

  • Septic sera induces apoptosis and DNA fragmentation factor 40 activation in fibroblasts.

    abstract::Sepsis, the systemic response to infection, is the leading cause of death in the intensive care units worldwide. Septic patients can succumb through the development of early refractory hypotension or late multiple organ dysfunction. Misregulation of apoptosis during sepsis may contribute to cellular dysfunction and mu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Brabant D,Michael P,Bleiblo F,Saleh M,Narain R,Tai TC,Ramana CV,Parrillo JE,Kumar A,Kumar A

    更新日期:2011-08-26 00:00:00

  • Non-lysosomal degradation pathway for N-linked glycans and dolichol-linked oligosaccharides.

    abstract::There is growing evidence that asparagine (N)-linked glycans play pivotal roles in protein folding and intra- or intercellular trafficking of N-glycosylated proteins. During the N-glycosylation of proteins, significant amounts of free oligosaccharides (fOSs) and phosphorylated oligosaccharides (POSs) are generated at ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Suzuki T,Harada Y

    更新日期:2014-10-17 00:00:00

  • Caught in the act: in vivo molecular imaging of the transcription factor NF-kappaB after myocardial infarction.

    abstract::Nuclear factor kappaB (NF-kappaB) is a ubiquitous transcription factor activated by various stimuli implicated in heart failure progression. However, its activation in heart failure has not been well defined yet. Therefore, we investigated activation of NF-kappaB after myocardial infarction. For the first time, we per...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tillmanns J,Carlsen H,Blomhoff R,Valen G,Calvillo L,Ertl G,Bauersachs J,Frantz S

    更新日期:2006-04-14 00:00:00

  • Localization of a negative vitamin D response sequence in the human growth hormone gene.

    abstract::1,25-Dihydroxyvitamin D(3) [1,25(OH)(2) D(3)] exerts its biological effects by binding to the vitamin D receptor (VDR), which binds in turn to the vitamin D response elements located in the target gene's promoter. We have previously demonstrated that VDR binds in vitro with high affinity to the 5'-flanking sequence of...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Seoane S,Alonso M,Segura C,Pérez-Fernández R

    更新日期:2002-03-22 00:00:00

  • Binding of a high reactive heparin to human apolipoprotein E: identification of two heparin-binding domains.

    abstract::Ligand-blotting and dot-blotting procedures were used to investigate the binding of [125I]-heparin to apolipoprotein E, its thrombin fragments E22 (residues 1-191) and E12 (residues 192-299), and to nine apolipoprotein E synthetic fragments. E22 and E12 bound [125I] heparin indicating multiple heparin-binding domains....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Cardin AD,Hirose N,Blankenship DT,Jackson RL,Harmony JA,Sparrow DA,Sparrow JT

    更新日期:1986-01-29 00:00:00

  • Sodium-dependent, concentrative nucleoside transport in cultured intestinal epithelial cells.

    abstract::In a simple salts medium, monolayers of IEC-6 intestinal cells achieved concentrations of unmetabolized formycin B (an analog of inosine) about 6-fold higher than in the medium. Rates of formycin B influx were a saturable function of Na+ concentrations in the medium. Although IEC-6 cells possess sites with high affini...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jakobs ES,Paterson AR

    更新日期:1986-11-14 00:00:00

  • Identification of poly(ADP-ribose) polymerase-1 as the OXPHOS-generated ATP sensor of nuclei of animal cells.

    abstract::Our results show that in the intact normal animal cell mitochondrial ATP is directly connected to nuclear PARP-1 by way of a specific adenylate kinase enzymatic path. This mechanism is demonstrated in two models: (a) by its inhibition with a specific inhibitor of adenylate kinase, and (b) by disruption of ATP synthesi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kun E,Kirsten E,Hakam A,Bauer PI,Mendeleyev J

    更新日期:2008-02-08 00:00:00

  • Heat shock cognate protein 70 is a cell fusion-enhancing factor but not an entry factor for human T-cell lymphotropic virus type I.

    abstract::Heat shock cognate protein 70 (HSC70) has been shown to bind to the peptide corresponding to amino acids 197 to 216 of human T-cell lymphotropic virus type I (HTLV-I) envelope protein, gp46, and an anti-HSC70 monoclonal antibody (mAb) inhibits HTLV-I-induced syncytium formation. These findings suggest that HSC70 is ne...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fang D,Haraguchi Y,Jinno A,Soda Y,Shimizu N,Hoshino H

    更新日期:1999-08-02 00:00:00

  • The enantioselective participation of (S)- and (R)-diaminovaleric acids in the formation of delta-aminolevulinic acid in cyanobacteria.

    abstract::Although it is recognized that 4,5-diaminovaleric acid, formed from glutamate 1-semialdehyde, functions as the intermediate in the last step of delta-aminolevulinic acid formation from glutamate, the enantioselectivity of the participating glutamate 1-semialdehyde aminotransferase for 4,5-diaminovaleric acid has remai...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Friedmann HC,Duban ME,Valasinas A,Frydman B

    更新日期:1992-05-29 00:00:00

  • Effect of oxidation of the non-catalytic β-propeller domain on the substrate specificity of prolyl oligopeptidase from Pleurotus eryngii.

    abstract::Enzymes belonging to the S9 family of prolyl oligopeptidases are of interest because of their pharmacological importance and have a non-catalytic β-propeller domain. In this study, we found that the oxidation of Met203, which lies on surface of the β-propeller domain, leads to change in the substrate specificity of er...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tokai S,Bito T,Shimizu K,Arima J

    更新日期:2017-05-27 00:00:00

  • Identification of the up- and down-regulated genes in vancomycin-resistant Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential hybridization method.

    abstract::We previously reported the first vancomycin-resistant Staphylococcus aureus (VRSA) clinical strain, Mu50, whose cell wall is remarkably thickened resulting from the activation of cell-wall synthesis. To explore the genetic basis for the vancomycin resistance, cDNA differential hybridization was performed using RNAs ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuroda M,Kuwahara-Arai K,Hiramatsu K

    更新日期:2000-03-16 00:00:00

  • Anti-HIV and anti-HBV activity and resistance profile of 2',3'-dideoxy-3'-thiacytidine (3TC) and its arylphosphoramidate derivative CF 1109.

    abstract::A novel membrane-soluble prodrug of the 5'-monophosphate derivative of 3TC containing a phenyl group and the methyl ester of L-alanine linked to the phosphorus through a phosphoramidate bond with the primary amino moiety (designated Cf 1109) was prepared. The 3TC prodrug proved less potent an inhibitor of HIV-1 and HI...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Balzarini J,Wedgwood O,Kruining J,Pelemans H,Heijtink R,De Clercq E,McGuigan C

    更新日期:1996-08-14 00:00:00

  • Next-generation sequencing-based analysis of reverse transcriptase fidelity.

    abstract::In this study, we devised a simple and rapid method to analyze fidelity of reverse transcriptase (RT) using next-generation sequencing (NGS). The method comprises a cDNA synthesis reaction from standard RNA with a primer containing a tag of 14 randomized bases and the RT to be tested, PCR using high-fidelity DNA polym...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yasukawa K,Iida K,Okano H,Hidese R,Baba M,Yanagihara I,Kojima K,Takita T,Fujiwara S

    更新日期:2017-10-14 00:00:00

  • Mature interleukin-33 is produced by calpain-mediated cleavage in vivo.

    abstract::Interleukin (IL)-33 is a novel member of the IL-1 family. IL-33 is primarily synthesized as a 30-kDa precursor (pro-IL-33). Pro-IL-33 is cleaved by caspase-1 into an 18-kDa mature form (mature IL-33) in vitro. Recombinant mature IL-33 has been known to induce T-helper type-2 (Th2)-associated cytokines and inflammatory...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hayakawa M,Hayakawa H,Matsuyama Y,Tamemoto H,Okazaki H,Tominaga S

    更新日期:2009-09-11 00:00:00

  • The role of MAP4K3 in lifespan regulation of Caenorhabditis elegans.

    abstract::The TOR pathway is a kinase signaling pathway that regulates cellular growth and proliferation in response to nutrients and growth factors. TOR signaling is also important in lifespan regulation - when this pathway is inhibited, either naturally, by genetic mutation, or by pharmacological means, lifespan is extended. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Khan MH,Hart MJ,Rea SL

    更新日期:2012-08-24 00:00:00

  • Thr420 and Ser454 of ZmCCaMK play a crucial role in brassinosteroid-induced antioxidant defense in maize.

    abstract::Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play important roles in brassinosteroid (BR)-induced antioxidant defense and enhancing the tolerance of plants to drought stress. The autophosphorylation of CCaMK is a key step for the activation of CCaMK, thus promoting substrate phosphorylation. H...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu L,Han T,Liu W,Han G,Di P,Yu X,Yan J,Zhang A

    更新日期:2020-05-07 00:00:00

  • Automated quantification of FISH signals in urinary cells enables the assessment of chromosomal aberration patterns characteristic for bladder cancer.

    abstract::Targeting the centromeres of chromosomes 3, 7, 17 (CEP3, 7, 17) and the 9p21-locus (LSI9p21) for diagnosing bladder cancer (BC) is time- and cost-intensive and requires a manual investigation of the sample by a well-trained investigator thus overall limiting its use in clinical diagnostics and large-scaled epidemiolog...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Köhler CU,Martin L,Bonberg N,Behrens T,Deix T,Braun K,Noldus J,Jöckel KH,Erbel R,Sommerer F,Tannapfel A,Harth V,Käfferlein HU,Brüning T

    更新日期:2014-06-13 00:00:00

  • Enzymatic sulfation of salivary mucins: structural features of 35S-labeled oligosaccharides.

    abstract::Sulfotransferase activity catalyzing the transfer of sulfate ester group from 3'-phosphoadenosine-5'-phosphosulfate to salivary mucins was found associated with Golgi-rich membrane fraction of rat submandibular salivary glands. Alkaline borohydride reductive cleavage of the synthesized 35S-labeled glycoprotein led to ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Slomiany BL,Piotrowski J,Nishikawa H,Slomiany A

    更新日期:1988-11-30 00:00:00

  • A form of human basic fibroblast growth factor with an extended amino terminus.

    abstract::The amino acid sequence of a human placental bFGF was determined by a combination of protein and cDNA sequencing. The placental bFGF consists of 157 amino acid residues with a calculated molecular weight of 17,464 and is highly homologous to bovine pituitary bFGF. The human protein contains an amino terminal extension...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sommer A,Brewer MT,Thompson RC,Moscatelli D,Presta M,Rifkin DB

    更新日期:1987-04-29 00:00:00

  • Conserved signal peptide of Notch3 inhibits interaction with proteasome.

    abstract::The Notch3 N-terminal sequence is conserved across several mammalian species but diverges from the three other Notch proteins. We determined the significance of the N-terminal sequence using deletion mutants. The first 39 amino acids are required for Notch3 receptor expression, processing, and functional activity. In ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang Y,Jia L,Lee SJ,Wang MM

    更新日期:2007-03-30 00:00:00

  • Interrelationship between activation of matrix metalloproteinases and mitochondrial dysfunction in the development of diabetic retinopathy.

    abstract::Mitochondria dysfunction plays a significant role in the apoptosis of retinal cells. Diabetes activates retinal matrix metalloproteinases (MMP-9 and MMP-2), damages retinal mitochondria and activates the apoptotic machinery. This study is to investigate the temporal relationship between the activation of retinal MMPs ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Santos JM,Tewari S,Lin JY,Kowluru RA

    更新日期:2013-09-06 00:00:00

  • Inhibitors of protein phosphatases 1 and 2A differentially prevent intrinsic and extrinsic apoptosis pathways.

    abstract::Inhibitors of serine/threonine protein phosphatases can inhibit apoptosis. We investigated which protein phosphatases are critical for this protection using calyculin A, okadaic acid, and tautomycin. All three phosphatase inhibitors prevented anisomycin-induced apoptosis in leukemia cell models. In vitro, calyculin A ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chatfield K,Eastman A

    更新日期:2004-10-29 00:00:00

  • Different microvascular permeability responses elicited by the CXC chemokines MIP-2 and KC during leukocyte recruitment: role of LSP1.

    abstract::The CXC chemokines keratinocyte-derived chemokine (KC, CXCL1) and macrophage inflammatory protein-2 (MIP-2, CXCL2) activate G-protein coupled receptor CXCR2 and are believed to have similar inflammatory effects in mice. Their specific signaling mechanisms remain elusive. A wide variety of cellular events, mediators an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lei X,Hossain M,Qadri SM,Liu L

    更新日期:2012-07-06 00:00:00