Abstract:
:Fluorescein-labeled antisense oligodeoxynucleotides (ODNs) corresponding to the promoters of the protooncogene c-myb (24-mer) and the oncogene c-myc (15-mer) were transported through the human skin in vitro by electroporation. Fluxes of 6.4 +/- 2.1 pM/cm2* hr and 11.5 +/- 3.5 pM/cm2* hr, respectively, were achieved during exponential pulsing [tau pulse = 1.1ms, transdermal voltage (Uskin) = 80V] every five seconds. The flux for Uskin < 70V was undetectable, rapidly increased at 80V, but plateaued at higher values. Fluorescence imaging demonstrated that transport of the ODNs is concentrated in localized transport regions (LTRs)1 approximately 30 microns in diameter.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Zewert TE,Pliquett UF,Langer R,Weaver JCdoi
10.1006/bbrc.1995.1968subject
Has Abstractpub_date
1995-07-17 00:00:00pages
286-92issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(85)71968-7journal_volume
212pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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