Synthesis and biological properties of antiparallel and parallel dimers of alpha-human atrial natriuretic peptide.

Abstract:

:To obtain antiparallel and parallel dimers of alpha-human atrial natriuretic peptide (alpha-hANP), two fully protected peptides I and II having the same amino acid sequence as alpha-hANP with different protective groups at the cysteinyl residues were synthesized, the former having Acm and Npys and the latter MeBzl and Acm. Equivalent amounts of peptides I and II were mixed and subjected to HF deprotection. Next, the first disulfide bond was linked between the remaining Npys group in I and the liberated SH group in II to form a monodisulfide dimer. The second disulfide bond was formed within the newly formed dimer between the remaining Acm groups by treatment with iodine, giving an antiparallel dimer. The parallel dimer of alpha-hANP was synthesized similarly starting from the protected peptide II. These dimers could be clearly segregated on HPLC. The retention time on HPLC of the antiparallel dimer was identical with that of natural beta-hANP. Both dimers showed biological activities as high as one third to one sixth of alpha-hANP in smooth muscle spasmolytic activity, and almost the same level of natriuretic activity as alpha-hANP at a high dose (10 nmol/kg) but about one fifth the activity at a low dose (1 nmol/kg). In these assay systems, the antiparallel dimer showed a slower onset and a tendency of longer duration than alpha-hANP.

authors

Chino N,Yoshizawa-Kumagaye K,Noda Y,Watanabe TX,Kimura T,Sakakibara S

doi

10.1016/s0006-291x(86)80224-8

subject

Has Abstract

pub_date

1986-12-15 00:00:00

pages

665-72

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80224-8

journal_volume

141

pub_type

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