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mRNA but not plasmid DNA is efficiently transfected in murine J774A.1 macrophages.

Abstract：

:Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as compared to control pulsed macrophages. In contrast, introduction of DNA into J774A.1 cells caused apoptosis without expression of the gene of interest. Our results show that mRNA nucleofection is a new high-speed transfection method for macrophages.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Van De Parre TJ,Martinet W,Schrijvers DM,Herman AG,De Meyer GR

doi

10.1016/j.bbrc.2004.12.027

subject

Has Abstract

pub_date

2005-02-04 00:00:00

pages

356-60

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(04)02796-2

journal_volume

327

pub_type

杂志文章

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